Restriction map for
pF1KB8216
(Circular) (Six-base) MAPSORT of: pF1KB8216.seq Check: 2167 from: 1 to: 3758
pF1KB8216 3758 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 440 796 440
Size: 356 3402
Acc65I G'GTAC_C
Cuts at: 774 774
Size: 3758
AclI AA'CG_TT
Cuts at: 3598 3598
Size: 3758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 724 1583 2015 2762 3030 440
Size: 284 859 432 747 268 1168
Fragments arranged by size:
1168 859 747 432 284 268
AflIII A'CryG_T
Cuts at: 1110 2214 3191 1110
Size: 1104 977 1677
Fragments arranged by size:
1677 1104 977
AgeI A'CCGG_T
Cuts at: 2146 2146
Size: 3758
AlwNI CAG_nnn'CTG
Cuts at: 181 193 277 526 1063 2630 181
Size: 12 84 249 537 1567 1309
Fragments arranged by size:
1567 1309 537 249 84 12
ApaLI G'TGCA_C
Cuts at: 2528 2528
Size: 3758
ApoI r'AATT_y
Cuts at: 532 762 3111 3367 532
Size: 230 2349 256 923
Fragments arranged by size:
2349 923 256 230
AseI AT'TA_AT
Cuts at: 20 20
Size: 3758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
AvaI C'yCGr_G
Cuts at: 778 778
Size: 3758
BamHI G'GATC_C
Cuts at: 783 783
Size: 3758
BanI G'GyrC_C
Cuts at: 774 1188 1436 1471 774
Size: 414 248 35 3061
Fragments arranged by size:
3061 414 248 35
BanII G_rGCy'C
Cuts at: 451 702 772 1802 451
Size: 251 70 1030 2407
Fragments arranged by size:
2407 1030 251 70
BbeI G_GCGC'C
Cuts at: 1192 1440 1192
Size: 248 3510
BbsI GAAGACnn'nnnn_
Cuts at: 352 352
Size: 3758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 659 693 90
Size: 34 535 34 3155
Fragments arranged by size:
3155 535 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1656 2417 3758 1656
Size: 761 1341 1656
Fragments arranged by size:
1656 1341 761
BclI T'GATC_A
Cuts at: 1278 1278
Size: 3758
BglI GCCn_nnn'nGGC
Cuts at: 265 265
Size: 3758
BglII A'GATC_T
Cuts at: 1273 3310 1273
Size: 2037 1721
BlpI GC'TnA_GC
Cuts at: 870 870
Size: 3758
Bme1580I G_kGCm'C
Cuts at: 1383 1476 2532 1383
Size: 93 1056 2609
Fragments arranged by size:
2609 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 364 970 1051 1386 3530 364
Size: 606 81 335 2144 592
Fragments arranged by size:
2144 606 592 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 3758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 1813 2305 2603 934
Size: 879 492 298 2089
Fragments arranged by size:
2089 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3462 3462
Size: 3758
BsaAI yAC'GTr
Cuts at: 1111 1111
Size: 3758
BsaBI GATnn'nnATC
Cuts at: 6 1272 3309 6
Size: 1266 2037 455
Fragments arranged by size:
2037 1266 455
BsaHI Gr'CG_yC
Cuts at: 1189 1437 2139 3635 1189
Size: 248 702 1496 1312
Fragments arranged by size:
1496 1312 702 248
BsaWI w'CCGG_w
Cuts at: 395 947 1468 2146 2420 2567 2978 395
Size: 552 521 678 274 147 411 1175
Fragments arranged by size:
1175 678 552 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 567 597 567
Size: 30 3728
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 613 613
Size: 3758
BseYI C'CCAG_C
Cuts at: 260 503 1183 1941 2518 3014 260
Size: 243 680 758 577 496 1004
Fragments arranged by size:
1004 758 680 577 496 243
BsiEI CG_ry'CG
Cuts at: 104 1300 1346 2554 104
Size: 1196 46 1208 1308
Fragments arranged by size:
1308 1208 1196 46
BsiHKAI G_wGCw'C
Cuts at: 772 1550 1740 2532 772
Size: 778 190 792 1998
Fragments arranged by size:
1998 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 451 702 772 1383 1476 1550 1740 1802 2532
Size: 251 70 611 93 74 190 62 730
Cuts at: 2532 451
Size: 1677
Fragments arranged by size:
1677 730 611 251 190 93 74 70
62
BspEI T'CCGG_A
Cuts at: 395 2978 395
Size: 2583 1175
BspHI T'CATG_A
Cuts at: 2934 2934
Size: 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 357 808 1022 1324 1705 357
Size: 451 214 302 381 2410
Fragments arranged by size:
2410 451 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2050 2104 3588 3753 50
Size: 2000 54 1484 165 55
Fragments arranged by size:
2000 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1670 1670
Size: 3758
BsrFI r'CCGG_y
Cuts at: 1756 1937 2146 1756
Size: 181 209 3368
Fragments arranged by size:
3368 209 181
BsrGI T'GTAC_A
Cuts at: 591 591
Size: 3758
BssSI C'ACGA_G
Cuts at: 2029 2387 2029
Size: 358 3400
BstAPI GCAn_nnn'nTGC
Cuts at: 153 1135 153
Size: 982 2776
BstBI TT'CG_AA
Cuts at: 2119 2119
Size: 3758
BstYI r'GATC_y
Cuts at: 1 783 1250 1273 1608 1854 2866 2952 2964
Size: 782 467 23 335 246 1012 86 12
Cuts at: 2964 3310 1
Size: 346 449
Fragments arranged by size:
1012 782 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1708 1889 2052 1708
Size: 181 163 3414
Fragments arranged by size:
3414 181 163
BtsI GCAGTG_nn'
Cuts at: 1001 1001
Size: 3758
ClaI AT'CG_AT
Cuts at: 284 284
Size: 3758
DraI TTT'AAA
Cuts at: 757 757
Size: 3758
DrdI GACnn_nn'nnGTC
Cuts at: 1464 2322 1464
Size: 858 2900
EaeI y'GGCC_r
Cuts at: 1343 1517 1908 1935 3280 1343
Size: 174 391 27 1345 1821
Fragments arranged by size:
1821 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1343 1343
Size: 3758
EarI CTCTTCn'nnn_
Cuts at: 602 608 1781 1991 602
Size: 6 1173 210 2369
Fragments arranged by size:
2369 1173 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1966 2276 2422 3568 1966
Size: 310 146 1146 2156
Fragments arranged by size:
2156 1146 310 146
EcoICRI GAG'CTC
Cuts at: 770 770
Size: 3758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 724 1583 2015 2762 3030 440
Size: 284 859 432 747 268 1168
Fragments arranged by size:
1168 859 747 432 284 268
EcoO109I rG'GnC_Cy
Cuts at: 897 897
Size: 3758
EcoRI G'AATT_C
Cuts at: 762 762
Size: 3758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1777 1777
Size: 3758
FspI TGC'GCA
Cuts at: 1539 1539
Size: 3758
HaeII r_GCGC'y
Cuts at: 1192 1440 2462 3441 1192
Size: 248 1022 979 1509
Fragments arranged by size:
1509 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 377 409 1600 1630 1632 1662 163
Size: 32 182 32 1191 30 2 30 2259
Fragments arranged by size:
2259 1191 182 32 32 30 30 2
HincII GTy'rAC
Cuts at: 797 797
Size: 3758
KasI G'GCGC_C
Cuts at: 1188 1436 1188
Size: 248 3510
KpnI G_GTAC'C
Cuts at: 778 778
Size: 3758
MluI A'CGCG_T
Cuts at: 3191 3191
Size: 3758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 298 610 2429 2613 298
Size: 312 1819 184 1443
Fragments arranged by size:
1819 1443 312 184
MscI TGG'CCA
Cuts at: 1519 1519
Size: 3758
MslI CAynn'nnrTG
Cuts at: 1747 1874 1747
Size: 127 3631
MspA1I CmG'CkG
Cuts at: 264 277 523 869 1183 1543 2556 2801 264
Size: 13 246 346 314 360 1013 245 1221
Fragments arranged by size:
1221 1013 360 346 314 246 245 13
NaeI GCC'GGC
Cuts at: 1939 1939
Size: 3758
NarI GG'CG_CC
Cuts at: 1189 1437 1189
Size: 248 3510
NgoMIV G'CCGG_C
Cuts at: 1937 1937
Size: 3758
NspI r_CATG'y
Cuts at: 811 1311 2218 811
Size: 500 907 2351
Fragments arranged by size:
2351 907 500
PciI A'CATG_T
Cuts at: 2214 2214
Size: 3758
PmeI GTTT'AAAC
Cuts at: 757 757
Size: 3758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 3726
PstI C_TGCA'G
Cuts at: 805 1490 805
Size: 685 3073
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3758
PvuII CAG'CTG
Cuts at: 264 277 523 1183 1543 264
Size: 13 246 660 360 2479
Fragments arranged by size:
2479 660 360 246 13
RsrII CG'GwC_CG
Cuts at: 1953 1953
Size: 3758
SacI G_AGCT'C
Cuts at: 772 772
Size: 3758
SalI G'TCGA_C
Cuts at: 795 795
Size: 3758
SapI GCTCTTCn'nnn_
Cuts at: 1781 1991 1781
Size: 210 3548
SbfI CC_TGCA'GG
Cuts at: 805 805
Size: 3758
ScaI AGT'ACT
Cuts at: 3275 3275
Size: 3758
SfcI C'TryA_G
Cuts at: 33 140 801 1486 2479 2670 33
Size: 107 661 685 993 191 1121
Fragments arranged by size:
1121 993 685 661 191 107
SfoI GGC'GCC
Cuts at: 1190 1438 1190
Size: 248 3510
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
SmaI CCC'GGG
Cuts at: 780 780
Size: 3758
SmlI C'TyrA_G
Cuts at: 913 1828 2320 2582 913
Size: 915 492 262 2089
Fragments arranged by size:
2089 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3758
SphI G_CATG'C
Cuts at: 811 1311 811
Size: 500 3258
StyI C'CwwG_G
Cuts at: 892 1246 892
Size: 354 3404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 253 1341 1474 1933 2142 253
Size: 1088 133 459 209 1869
Fragments arranged by size:
1869 1088 459 209 133
TatI w'GTAC_w
Cuts at: 591 3273 591
Size: 2682 1076
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 567 599 3275 3307 567
Size: 32 2676 32 1018
Fragments arranged by size:
2676 1018 32 32
Tth111I GACn'n_nGTC
Cuts at: 177 1555 177
Size: 1378 2380
XbaI T'CTAG_A
Cuts at: 789 789
Size: 3758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1253 1253
Size: 3758
XmaI C'CCGG_G
Cuts at: 778 778
Size: 3758
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
BpmI Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI