Restriction map for
pF1KB8212
(Circular) (Six-base) MAPSORT of: pF1KB8212.seq Check: 7200 from: 1 to: 3515
pF1KB8212 3515 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 553 553
Size: 3515
Acc65I G'GTAC_C
Cuts at: 531 531
Size: 3515
AclI AA'CG_TT
Cuts at: 3355 3355
Size: 3515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 406 437 1340 1772 2519 2787 251
Size: 155 31 903 432 747 268 979
Fragments arranged by size:
979 903 747 432 268 155 31
AflIII A'CryG_T
Cuts at: 113 867 1971 2948 113
Size: 754 1104 977 680
Fragments arranged by size:
1104 977 754 680
AgeI A'CCGG_T
Cuts at: 1903 1903
Size: 3515
AlwNI CAG_nnn'CTG
Cuts at: 274 820 2387 274
Size: 546 1567 1402
Fragments arranged by size:
1567 1402 546
ApaLI G'TGCA_C
Cuts at: 2285 2285
Size: 3515
ApoI r'AATT_y
Cuts at: 370 519 2868 3124 370
Size: 149 2349 256 761
Fragments arranged by size:
2349 761 256 149
AseI AT'TA_AT
Cuts at: 20 20
Size: 3515
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
AvaI C'yCGr_G
Cuts at: 239 535 239
Size: 296 3219
BamHI G'GATC_C
Cuts at: 540 540
Size: 3515
BanI G'GyrC_C
Cuts at: 531 945 1193 1228 531
Size: 414 248 35 2818
Fragments arranged by size:
2818 414 248 35
BanII G_rGCy'C
Cuts at: 437 529 1559 437
Size: 92 1030 2393
Fragments arranged by size:
2393 1030 92
BbeI G_GCGC'C
Cuts at: 949 1197 949
Size: 248 3267
BbsI GAAGACnn'nnnn_
Cuts at: 208 208
Size: 3515
BciVI GTATCCnnnnn_n'
Cuts at: 1413 2174 3515 1413
Size: 761 1341 1413
Fragments arranged by size:
1413 1341 761
BclI T'GATC_A
Cuts at: 1035 1035
Size: 3515
BglII A'GATC_T
Cuts at: 1030 3067 1030
Size: 2037 1478
BlpI GC'TnA_GC
Cuts at: 627 627
Size: 3515
Bme1580I G_kGCm'C
Cuts at: 1140 1233 2289 1140
Size: 93 1056 2366
Fragments arranged by size:
2366 1056 93
BmgBI CAC'GTC
Cuts at: 114 114
Size: 3515
BmrI ACTGGGnnnn_n'
Cuts at: 727 808 1143 3287 727
Size: 81 335 2144 955
Fragments arranged by size:
2144 955 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1570 2062 2360 691
Size: 879 492 298 1846
Fragments arranged by size:
1846 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3219 3219
Size: 3515
BsaAI yAC'GTr
Cuts at: 868 868
Size: 3515
BsaBI GATnn'nnATC
Cuts at: 6 1029 3066 6
Size: 1023 2037 455
Fragments arranged by size:
2037 1023 455
BsaHI Gr'CG_yC
Cuts at: 215 946 1194 1896 3392 215
Size: 731 248 702 1496 338
Fragments arranged by size:
1496 731 702 338 248
BsaWI w'CCGG_w
Cuts at: 704 1225 1903 2177 2324 2735 704
Size: 521 678 274 147 411 1484
Fragments arranged by size:
1484 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 299 329 299
Size: 30 3485
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 323 323
Size: 3515
BseYI C'CCAG_C
Cuts at: 940 1698 2275 2771 940
Size: 758 577 496 1684
Fragments arranged by size:
1684 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1057 1103 2311 104
Size: 953 46 1208 1308
Fragments arranged by size:
1308 1208 953 46
BsiHKAI G_wGCw'C
Cuts at: 529 1307 1497 2289 529
Size: 778 190 792 1755
Fragments arranged by size:
1755 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 437 529 1140 1233 1307 1497 1559 2289 437
Size: 92 611 93 74 190 62 730 1663
Fragments arranged by size:
1663 730 611 190 93 92 74 62
BspEI T'CCGG_A
Cuts at: 2735 2735
Size: 3515
BspHI T'CATG_A
Cuts at: 119 2691 119
Size: 2572 943
BspMI ACCTGCnnnn'nnnn_
Cuts at: 565 779 1081 1462 565
Size: 214 302 381 2618
Fragments arranged by size:
2618 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1807 1861 3345 3510 50
Size: 1757 54 1484 165 55
Fragments arranged by size:
1757 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1427 1427
Size: 3515
BsrFI r'CCGG_y
Cuts at: 1513 1694 1903 1513
Size: 181 209 3125
Fragments arranged by size:
3125 209 181
BssSI C'ACGA_G
Cuts at: 1786 2144 1786
Size: 358 3157
BstAPI GCAn_nnn'nTGC
Cuts at: 892 892
Size: 3515
BstBI TT'CG_AA
Cuts at: 1876 1876
Size: 3515
BstEII G'GTnAC_C
Cuts at: 183 183
Size: 3515
BstXI CCAn_nnnn'nTGG
Cuts at: 354 354
Size: 3515
BstYI r'GATC_y
Cuts at: 1 540 1007 1030 1365 1611 2623 2709 2721
Size: 539 467 23 335 246 1012 86 12
Cuts at: 2721 3067 1
Size: 346 449
Fragments arranged by size:
1012 539 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 134 161 107
Size: 27 27 3461
Fragments arranged by size:
3461 27 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 321 1465 1646 1809 321
Size: 1144 181 163 2027
Fragments arranged by size:
2027 1144 181 163
BtsI GCAGTG_nn'
Cuts at: 758 758
Size: 3515
DraI TTT'AAA
Cuts at: 514 514
Size: 3515
DrdI GACnn_nn'nnGTC
Cuts at: 1221 2079 1221
Size: 858 2657
EaeI y'GGCC_r
Cuts at: 137 1100 1274 1665 1692 3037 137
Size: 963 174 391 27 1345 615
Fragments arranged by size:
1345 963 615 391 174 27
EagI C'GGCC_G
Cuts at: 1100 1100
Size: 3515
EarI CTCTTCn'nnn_
Cuts at: 355 1538 1748 355
Size: 1183 210 2122
Fragments arranged by size:
2122 1183 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 211 1723 2033 2179 3325 211
Size: 1512 310 146 1146 401
Fragments arranged by size:
1512 1146 401 310 146
EcoICRI GAG'CTC
Cuts at: 527 527
Size: 3515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 406 437 1340 1772 2519 2787 251
Size: 155 31 903 432 747 268 979
Fragments arranged by size:
979 903 747 432 268 155 31
EcoO109I rG'GnC_Cy
Cuts at: 126 468 654 126
Size: 342 186 2987
Fragments arranged by size:
2987 342 186
EcoRI G'AATT_C
Cuts at: 370 519 370
Size: 149 3366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1534 1534
Size: 3515
FspI TGC'GCA
Cuts at: 1296 1296
Size: 3515
HaeII r_GCGC'y
Cuts at: 949 1197 2219 3198 949
Size: 248 1022 979 1266
Fragments arranged by size:
1266 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1357 1387 1389 1419 1357
Size: 30 2 30 3453
Fragments arranged by size:
3453 30 30 2
HincII GTy'rAC
Cuts at: 554 554
Size: 3515
KasI G'GCGC_C
Cuts at: 945 1193 945
Size: 248 3267
KpnI G_GTAC'C
Cuts at: 535 535
Size: 3515
MluI A'CGCG_T
Cuts at: 2948 2948
Size: 3515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 207 2186 2370 207
Size: 1979 184 1352
Fragments arranged by size:
1979 1352 184
MscI TGG'CCA
Cuts at: 139 1276 139
Size: 1137 2378
MslI CAynn'nnrTG
Cuts at: 112 1504 1631 112
Size: 1392 127 1996
Fragments arranged by size:
1996 1392 127
MspA1I CmG'CkG
Cuts at: 163 626 940 1300 2313 2558 163
Size: 463 314 360 1013 245 1120
Fragments arranged by size:
1120 1013 463 360 314 245
NaeI GCC'GGC
Cuts at: 1696 1696
Size: 3515
NarI GG'CG_CC
Cuts at: 946 1194 946
Size: 248 3267
NcoI C'CATG_G
Cuts at: 107 134 107
Size: 27 3488
NgoMIV G'CCGG_C
Cuts at: 1694 1694
Size: 3515
NspI r_CATG'y
Cuts at: 568 1068 1975 568
Size: 500 907 2108
Fragments arranged by size:
2108 907 500
PciI A'CATG_T
Cuts at: 1971 1971
Size: 3515
PmeI GTTT'AAAC
Cuts at: 514 514
Size: 3515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2654 2686 2654
Size: 32 3483
PpuMI rG'GwC_Cy
Cuts at: 468 468
Size: 3515
PstI C_TGCA'G
Cuts at: 562 1247 562
Size: 685 2830
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3515
PvuII CAG'CTG
Cuts at: 940 1300 940
Size: 360 3155
RsrII CG'GwC_CG
Cuts at: 1710 1710
Size: 3515
SacI G_AGCT'C
Cuts at: 529 529
Size: 3515
SacII CC_GC'GG
Cuts at: 164 164
Size: 3515
SalI G'TCGA_C
Cuts at: 552 552
Size: 3515
SapI GCTCTTCn'nnn_
Cuts at: 1538 1748 1538
Size: 210 3305
SbfI CC_TGCA'GG
Cuts at: 562 562
Size: 3515
ScaI AGT'ACT
Cuts at: 3032 3032
Size: 3515
SfcI C'TryA_G
Cuts at: 33 558 1243 2236 2427 33
Size: 525 685 993 191 1121
Fragments arranged by size:
1121 993 685 525 191
SfoI GGC'GCC
Cuts at: 947 1195 947
Size: 248 3267
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
SmaI CCC'GGG
Cuts at: 537 537
Size: 3515
SmlI C'TyrA_G
Cuts at: 670 1585 2077 2339 670
Size: 915 492 262 1846
Fragments arranged by size:
1846 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3515
SphI G_CATG'C
Cuts at: 568 1068 568
Size: 500 3015
StuI AGG'CCT
Cuts at: 295 295
Size: 3515
StyI C'CwwG_G
Cuts at: 107 134 140 206 284 649 1003 107
Size: 27 6 66 78 365 354 2619
Fragments arranged by size:
2619 365 354 78 66 27 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1098 1231 1690 1899 1098
Size: 133 459 209 2714
Fragments arranged by size:
2714 459 209 133
TatI w'GTAC_w
Cuts at: 3030 3030
Size: 3515
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 331 3032 3064 299
Size: 32 2701 32 750
Fragments arranged by size:
2701 750 32 32
Tth111I GACn'n_nGTC
Cuts at: 1312 1312
Size: 3515
XbaI T'CTAG_A
Cuts at: 546 546
Size: 3515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1010 1010
Size: 3515
XmaI C'CCGG_G
Cuts at: 535 535
Size: 3515
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI