Restriction map for
pF1KB8210
(Circular) (Six-base) MAPSORT of: pF1KB8210.seq Check: 2095 from: 1 to: 4241
pF1KB8210 4241 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 2498 3245 3513 2066
Size: 432 747 268 2794
Fragments arranged by size:
2794 747 432 268
AflIII A'CryG_T
Cuts at: 1593 2697 3674 1593
Size: 1104 977 2160
Fragments arranged by size:
2160 1104 977
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 1546 3113 1546
Size: 1567 2674
ApaLI G'TGCA_C
Cuts at: 3011 3011
Size: 4241
ApoI r'AATT_y
Cuts at: 811 955 1075 1245 3594 3850 811
Size: 144 120 170 2349 256 1202
Fragments arranged by size:
2349 1202 256 170 144 120
AseI AT'TA_AT
Cuts at: 20 331 20
Size: 311 3930
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 1261 1261
Size: 4241
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 1257 1671 1919 1954 1257
Size: 414 248 35 3544
Fragments arranged by size:
3544 414 248 35
BanII G_rGCy'C
Cuts at: 690 1255 2285 690
Size: 565 1030 2646
Fragments arranged by size:
2646 1030 565
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BfrBI ATG'CAT
Cuts at: 219 219
Size: 4241
BglI GCCn_nnn'nGGC
Cuts at: 368 368
Size: 4241
BglII A'GATC_T
Cuts at: 1223 1756 3793 1223
Size: 533 2037 1671
Fragments arranged by size:
2037 1671 533
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 1866 1959 3015 1866
Size: 93 1056 3092
Fragments arranged by size:
3092 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1534 1869 4013 1453
Size: 81 335 2144 1681
Fragments arranged by size:
2144 1681 335 81
BmtI G_CTAG'C
Cuts at: 394 394
Size: 4241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 671 446
Size: 225 4016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2296 2788 3086 1417
Size: 879 492 298 2572
Fragments arranged by size:
2572 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3945 3945
Size: 4241
BsaAI yAC'GTr
Cuts at: 1594 1594
Size: 4241
BsaBI GATnn'nnATC
Cuts at: 6 886 1755 3792 6
Size: 880 869 2037 455
Fragments arranged by size:
2037 880 869 455
BsaHI Gr'CG_yC
Cuts at: 1672 1920 2622 4118 1672
Size: 248 702 1496 1795
Fragments arranged by size:
1795 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 366 633 1666 2424 3001 3497 366
Size: 267 1033 758 577 496 1110
Fragments arranged by size:
1110 1033 758 577 496 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4241
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3037 104
Size: 1679 46 1208 1308
Fragments arranged by size:
1679 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1255 2033 2223 3015 1255
Size: 778 190 792 2481
Fragments arranged by size:
2481 792 778 190
BsmI GAATG_Cn'
Cuts at: 1167 1167
Size: 4241
Bsp1286I G_dGCh'C
Cuts at: 690 1255 1866 1959 2033 2223 2285 3015 690
Size: 565 611 93 74 190 62 730 1916
Fragments arranged by size:
1916 730 611 565 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 993 3417 993
Size: 2424 1817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 648 889 1095 1291 1505 1807 2188 278
Size: 370 241 206 196 214 302 381 2331
Fragments arranged by size:
2331 381 370 302 241 214 206 196
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 134 1040 2153 134
Size: 906 1113 2222
Fragments arranged by size:
2222 1113 906
BsrFI r'CCGG_y
Cuts at: 795 2239 2420 2629 795
Size: 1444 181 209 2407
Fragments arranged by size:
2407 1444 209 181
BssSI C'ACGA_G
Cuts at: 2512 2870 2512
Size: 358 3883
BstAPI GCAn_nnn'nTGC
Cuts at: 805 1618 805
Size: 813 3428
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstXI CCAn_nnnn'nTGG
Cuts at: 759 759
Size: 4241
BstYI r'GATC_y
Cuts at: 1 205 875 1154 1223 1266 1733 1756 2091
Size: 204 670 279 69 43 467 23 335
Cuts at: 2091 2337 3349 3435 3447 3793 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 670 467 449 346 335 279 246
204 86 69 43 23 12
BtgI C'CryG_G
Cuts at: 587 587
Size: 4241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 220 2191 2372 2535 220
Size: 1971 181 163 1926
Fragments arranged by size:
1971 1926 181 163
BtsI GCAGTG_nn'
Cuts at: 803 1484 803
Size: 681 3560
DraI TTT'AAA
Cuts at: 248 1240 248
Size: 992 3249
DrdI GACnn_nn'nnGTC
Cuts at: 1947 2805 1947
Size: 858 3383
EaeI y'GGCC_r
Cuts at: 749 1826 2000 2391 2418 3763 749
Size: 1077 174 391 27 1345 1227
Fragments arranged by size:
1345 1227 1077 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 2759 2905 4051 2449
Size: 310 146 1146 2639
Fragments arranged by size:
2639 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 671 2066 2498 3245 3513 446
Size: 225 1395 432 747 268 1174
Fragments arranged by size:
1395 1174 747 432 268 225
EcoNI CCTnn'n_nnAGG
Cuts at: 1201 1201
Size: 4241
EcoO109I rG'GnC_Cy
Cuts at: 637 1380 637
Size: 743 3498
EcoRI G'AATT_C
Cuts at: 955 1245 955
Size: 290 3951
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 1675 1923 2945 3924 1675
Size: 248 1022 979 1992
Fragments arranged by size:
1992 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 432 514 546 975 1007 2083 2113 2115
Size: 32 82 32 429 32 1076 30 2
Cuts at: 2115 2145 400
Size: 30 2496
Fragments arranged by size:
2496 1076 429 82 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2912 3096 2912
Size: 184 4057
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 2230 2357 2230
Size: 127 4114
MspA1I CmG'CkG
Cuts at: 366 1352 1666 2026 3039 3284 366
Size: 986 314 360 1013 245 1323
Fragments arranged by size:
1323 1013 986 360 314 245
NaeI GCC'GGC
Cuts at: 797 2422 797
Size: 1625 2616
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NcoI C'CATG_G
Cuts at: 587 587
Size: 4241
NgoMIV G'CCGG_C
Cuts at: 795 2420 795
Size: 1625 2616
NheI G'CTAG_C
Cuts at: 390 390
Size: 4241
NsiI A_TGCA'T
Cuts at: 221 221
Size: 4241
NspI r_CATG'y
Cuts at: 1294 1794 2701 1294
Size: 500 907 2834
Fragments arranged by size:
2834 907 500
PasI CC'CwG_GG
Cuts at: 196 196
Size: 4241
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PflMI CCAn_nnn'nTGG
Cuts at: 202 1192 202
Size: 990 3251
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 4209
PpuMI rG'GwC_Cy
Cuts at: 637 637
Size: 4241
PsiI TTA'TAA
Cuts at: 971 971
Size: 4241
PstI C_TGCA'G
Cuts at: 1288 1973 1288
Size: 685 3556
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 366 1666 2026 366
Size: 1300 360 2581
Fragments arranged by size:
2581 1300 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 1284 1969 2962 3153 33
Size: 1251 685 993 191 1121
Fragments arranged by size:
1251 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 1396 2311 2803 3065 1396
Size: 915 492 262 2572
Fragments arranged by size:
2572 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
StyI C'CwwG_G
Cuts at: 587 1375 1729 587
Size: 788 354 3099
Fragments arranged by size:
3099 788 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1000 1824 1957 2416 2625 1000
Size: 824 133 459 209 2616
Fragments arranged by size:
2616 824 459 209 133
TatI w'GTAC_w
Cuts at: 1174 3756 1174
Size: 2582 1659
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4209
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1736 1736
Size: 4241
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BmgBI
Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI PacI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI