Restriction map for
pF1KB8209
(Circular) (Six-base) MAPSORT of: pF1KB8209.seq Check: 2418 from: 1 to: 3929
pF1KB8209 3929 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 480 967 480
Size: 487 3442
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 3769 3769
Size: 3929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1754 2186 2933 3201 1754
Size: 432 747 268 2482
Fragments arranged by size:
2482 747 432 268
AfeI AGC'GCT
Cuts at: 616 616
Size: 3929
AflIII A'CryG_T
Cuts at: 327 1281 2385 3362 327
Size: 954 1104 977 894
Fragments arranged by size:
1104 977 954 894
AgeI A'CCGG_T
Cuts at: 350 2317 350
Size: 1967 1962
AlwNI CAG_nnn'CTG
Cuts at: 385 765 1234 2801 385
Size: 380 469 1567 1513
Fragments arranged by size:
1567 1513 469 380
ApaI G_GGCC'C
Cuts at: 774 774
Size: 3929
ApaLI G'TGCA_C
Cuts at: 2699 2699
Size: 3929
ApoI r'AATT_y
Cuts at: 933 3282 3538 933
Size: 2349 256 1324
Fragments arranged by size:
2349 1324 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 949 949
Size: 3929
BamHI G'GATC_C
Cuts at: 954 954
Size: 3929
BanI G'GyrC_C
Cuts at: 499 945 1359 1607 1642 499
Size: 446 414 248 35 2786
Fragments arranged by size:
2786 446 414 248 35
BanII G_rGCy'C
Cuts at: 774 810 854 876 943 1973 774
Size: 36 44 22 67 1030 2730
Fragments arranged by size:
2730 1030 67 44 36 22
BbeI G_GCGC'C
Cuts at: 1363 1611 1363
Size: 248 3681
BbsI GAAGACnn'nnnn_
Cuts at: 324 324
Size: 3929
BbvCI CC'TCA_GC
Cuts at: 344 574 344
Size: 230 3699
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 104 138 104
Size: 34 3895
BciVI GTATCCnnnnn_n'
Cuts at: 1827 2588 3929 1827
Size: 761 1341 1827
Fragments arranged by size:
1827 1341 761
BclI T'GATC_A
Cuts at: 1449 1449
Size: 3929
BglI GCCn_nnn'nGGC
Cuts at: 112 725 112
Size: 613 3316
BglII A'GATC_T
Cuts at: 1444 3481 1444
Size: 2037 1892
BlpI GC'TnA_GC
Cuts at: 1041 1041
Size: 3929
Bme1580I G_kGCm'C
Cuts at: 504 774 1554 1647 2703 504
Size: 270 780 93 1056 1730
Fragments arranged by size:
1730 1056 780 270 93
BmgBI CAC'GTC
Cuts at: 291 799 291
Size: 508 3421
BmrI ACTGGGnnnn_n'
Cuts at: 1141 1222 1557 3701 1141
Size: 81 335 2144 1369
Fragments arranged by size:
2144 1369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 3929
Bpu10I CC'TnA_GC
Cuts at: 344 574 344
Size: 230 3699
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 620 639 1105 1984 2476 2774 526
Size: 94 19 466 879 492 298 1681
Fragments arranged by size:
1681 879 492 466 298 94 19
BsaI GGTCTCn'nnnn_
Cuts at: 643 3633 643
Size: 2990 939
BsaAI yAC'GTr
Cuts at: 1282 1282
Size: 3929
BsaBI GATnn'nnATC
Cuts at: 6 1443 3480 6
Size: 1437 2037 455
Fragments arranged by size:
2037 1437 455
BsaHI Gr'CG_yC
Cuts at: 1360 1608 2310 3806 1360
Size: 248 702 1496 1483
Fragments arranged by size:
1496 1483 702 248
BsaWI w'CCGG_w
Cuts at: 350 1118 1639 2317 2591 2738 3149 350
Size: 768 521 678 274 147 411 1130
Fragments arranged by size:
1130 768 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 202 199
Size: 3 3926
BseYI C'CCAG_C
Cuts at: 743 1354 2112 2689 3185 743
Size: 611 758 577 496 1487
Fragments arranged by size:
1487 758 611 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 271
Size: 3929
BsiEI CG_ry'CG
Cuts at: 104 701 1471 1517 2725 104
Size: 597 770 46 1208 1308
Fragments arranged by size:
1308 1208 770 597 46
BsiHKAI G_wGCw'C
Cuts at: 584 943 1721 1911 2703 584
Size: 359 778 190 792 1810
Fragments arranged by size:
1810 792 778 359 190
BsmBI CGTCTCn'nnnn_
Cuts at: 354 354
Size: 3929
Bsp1286I G_dGCh'C
Cuts at: 504 584 774 810 854 876 943 1554 1647
Size: 80 190 36 44 22 67 611 93
Cuts at: 1647 1721 1911 1973 2703 504
Size: 74 190 62 730 1730
Fragments arranged by size:
1730 730 611 190 190 93 80 74
67 62 44 36 22
BspEI T'CCGG_A
Cuts at: 3149 3149
Size: 3929
BspHI T'CATG_A
Cuts at: 3105 3105
Size: 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 979 1193 1495 1876 979
Size: 214 302 381 3032
Fragments arranged by size:
3032 381 302 214
BsrBI CCG'CTC
Cuts at: 50 625 839 2221 2275 3759 3924 50
Size: 575 214 1382 54 1484 165 55
Fragments arranged by size:
1484 1382 575 214 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1841 1841
Size: 3929
BsrFI r'CCGG_y
Cuts at: 350 1927 2108 2317 350
Size: 1577 181 209 1962
Fragments arranged by size:
1962 1577 209 181
BssSI C'ACGA_G
Cuts at: 2200 2558 2200
Size: 358 3571
BstAPI GCAn_nnn'nTGC
Cuts at: 385 1306 385
Size: 921 3008
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstXI CCAn_nnnn'nTGG
Cuts at: 468 468
Size: 3929
BstYI r'GATC_y
Cuts at: 1 863 954 1421 1444 1779 2025 3037 3123
Size: 862 91 467 23 335 246 1012 86
Cuts at: 3123 3135 3481 1
Size: 12 346 449
Fragments arranged by size:
1012 862 467 449 346 335 246 91
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3929
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 2060 2223 1879
Size: 181 163 3585
Fragments arranged by size:
3585 181 163
BtsI GCAGTG_nn'
Cuts at: 1172 1172
Size: 3929
DraI TTT'AAA
Cuts at: 928 928
Size: 3929
DrdI GACnn_nn'nnGTC
Cuts at: 1635 2493 1635
Size: 858 3071
EaeI y'GGCC_r
Cuts at: 473 698 787 857 1514 1688 2079 2106 3451
Size: 225 89 70 657 174 391 27 1345
Cuts at: 3451 473
Size: 951
Fragments arranged by size:
1345 951 657 391 225 174 89 70
27
EagI C'GGCC_G
Cuts at: 698 1514 698
Size: 816 3113
EarI CTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2137 2447 2593 3739 2137
Size: 310 146 1146 2327
Fragments arranged by size:
2327 1146 310 146
EcoICRI GAG'CTC
Cuts at: 941 941
Size: 3929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1754 2186 2933 3201 1754
Size: 432 747 268 2482
Fragments arranged by size:
2482 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 708 770 901 1068 708
Size: 62 131 167 3569
Fragments arranged by size:
3569 167 131 62
EcoRI G'AATT_C
Cuts at: 933 933
Size: 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3929
FspI TGC'GCA
Cuts at: 607 1710 607
Size: 1103 2826
HaeII r_GCGC'y
Cuts at: 311 600 618 1363 1611 2633 3612 311
Size: 289 18 745 248 1022 979 628
Fragments arranged by size:
1022 979 745 628 289 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1771 1801 1803 1833 1771
Size: 30 2 30 3867
Fragments arranged by size:
3867 30 30 2
HincII GTy'rAC
Cuts at: 968 968
Size: 3929
KasI G'GCGC_C
Cuts at: 1359 1607 1359
Size: 248 3681
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MluI A'CGCG_T
Cuts at: 327 3362 327
Size: 3035 894
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 303 866 2600 2784 303
Size: 563 1734 184 1448
Fragments arranged by size:
1734 1448 563 184
MscI TGG'CCA
Cuts at: 859 1690 859
Size: 831 3098
MslI CAynn'nnrTG
Cuts at: 493 1918 2045 493
Size: 1425 127 2377
Fragments arranged by size:
2377 1425 127
MspA1I CmG'CkG
Cuts at: 153 385 1040 1354 1714 2727 2972 153
Size: 232 655 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 655 360 314 245 232
NaeI GCC'GGC
Cuts at: 2110 2110
Size: 3929
NarI GG'CG_CC
Cuts at: 1360 1608 1360
Size: 248 3681
NcoI C'CATG_G
Cuts at: 107 107
Size: 3929
NgoMIV G'CCGG_C
Cuts at: 2108 2108
Size: 3929
NspI r_CATG'y
Cuts at: 982 1482 2389 982
Size: 500 907 2522
Fragments arranged by size:
2522 907 500
PciI A'CATG_T
Cuts at: 2385 2385
Size: 3929
PfoI T'CCnGG_A
Cuts at: 730 730
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 337 369 3068 3100 337
Size: 32 2699 32 1166
Fragments arranged by size:
2699 1166 32 32
PpuMI rG'GwC_Cy
Cuts at: 708 901 708
Size: 193 3736
PspOMI G'GGCC_C
Cuts at: 770 770
Size: 3929
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 529 561 529
Size: 32 3897
PstI C_TGCA'G
Cuts at: 976 1661 976
Size: 685 3244
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 385 1354 1714 385
Size: 969 360 2600
Fragments arranged by size:
2600 969 360
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 943 943
Size: 3929
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SanDI GG'GwC_CC
Cuts at: 708 708
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
SbfI CC_TGCA'GG
Cuts at: 976 976
Size: 3929
ScaI AGT'ACT
Cuts at: 547 3446 547
Size: 2899 1030
SexAI A'CCwGG_T
Cuts at: 562 562
Size: 3929
SfcI C'TryA_G
Cuts at: 33 972 1657 2650 2841 33
Size: 939 685 993 191 1121
Fragments arranged by size:
1121 993 939 685 191
SfoI GGC'GCC
Cuts at: 1361 1609 1361
Size: 248 3681
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SmaI CCC'GGG
Cuts at: 951 951
Size: 3929
SmlI C'TyrA_G
Cuts at: 541 618 635 1084 1999 2491 2753 541
Size: 77 17 449 915 492 262 1717
Fragments arranged by size:
1717 915 492 449 262 77 17
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 982 1482 982
Size: 500 3429
StyI C'CwwG_G
Cuts at: 107 413 1063 1417 107
Size: 306 650 354 2619
Fragments arranged by size:
2619 650 354 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 423 487 667 1512 1645 2104 2313 423
Size: 64 180 845 133 459 209 2039
Fragments arranged by size:
2039 845 459 209 180 133 64
TatI w'GTAC_w
Cuts at: 545 3444 545
Size: 2899 1030
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 3897
Tth111I GACn'n_nGTC
Cuts at: 1726 1726
Size: 3929
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 728 1424 728
Size: 696 3233
XmaI C'CCGG_G
Cuts at: 949 949
Size: 3929
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmtI BpmI BsaXI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspXI SacII SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI