Restriction map for
pF1KB8207
(Circular) (Six-base) MAPSORT of: pF1KB8207.seq Check: 4659 from: 1 to: 3989
pF1KB8207 3989 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 314 314
Size: 3989
AccI GT'mk_AC
Cuts at: 1027 1027
Size: 3989
Acc65I G'GTAC_C
Cuts at: 605 1005 605
Size: 400 3589
AclI AA'CG_TT
Cuts at: 3829 3829
Size: 3989
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 388 508 928 1814 2246 2993 3261 301
Size: 87 120 420 886 432 747 268 1029
Fragments arranged by size:
1029 886 747 432 420 268 120 87
AflIII A'CryG_T
Cuts at: 659 1341 2445 3422 659
Size: 682 1104 977 1226
Fragments arranged by size:
1226 1104 977 682
AgeI A'CCGG_T
Cuts at: 2377 2377
Size: 3989
AhdI GACnn_n'nnGTC
Cuts at: 663 663
Size: 3989
AlwNI CAG_nnn'CTG
Cuts at: 366 501 1294 2861 366
Size: 135 793 1567 1494
Fragments arranged by size:
1567 1494 793 135
ApaLI G'TGCA_C
Cuts at: 2759 2759
Size: 3989
ApoI r'AATT_y
Cuts at: 349 993 3342 3598 349
Size: 644 2349 256 740
Fragments arranged by size:
2349 740 644 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3989
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
AvaI C'yCGr_G
Cuts at: 551 1009 551
Size: 458 3531
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 589 622 589
Size: 33 3956
BamHI G'GATC_C
Cuts at: 1014 1014
Size: 3989
BanI G'GyrC_C
Cuts at: 145 605 1005 1419 1667 1702 145
Size: 460 400 414 248 35 2432
Fragments arranged by size:
2432 460 414 400 248 35
BanII G_rGCy'C
Cuts at: 1003 2033 1003
Size: 1030 2959
BbeI G_GCGC'C
Cuts at: 1423 1671 1423
Size: 248 3741
BbvCI CC'TCA_GC
Cuts at: 587 587
Size: 3989
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 711 745 90
Size: 34 587 34 3334
Fragments arranged by size:
3334 587 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1887 2648 3989 1887
Size: 761 1341 1887
Fragments arranged by size:
1887 1341 761
BclI T'GATC_A
Cuts at: 1509 1509
Size: 3989
BglII A'GATC_T
Cuts at: 260 1504 3541 260
Size: 1244 2037 708
Fragments arranged by size:
2037 1244 708
BlpI GC'TnA_GC
Cuts at: 1101 1101
Size: 3989
Bme1580I G_kGCm'C
Cuts at: 866 1614 1707 2763 866
Size: 748 93 1056 2092
Fragments arranged by size:
2092 1056 748 93
BmgBI CAC'GTC
Cuts at: 650 650
Size: 3989
BmrI ACTGGGnnnn_n'
Cuts at: 1201 1282 1617 3761 1201
Size: 81 335 2144 1429
Fragments arranged by size:
2144 1429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 3989
Bpu10I CC'TnA_GC
Cuts at: 587 587
Size: 3989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 1165 2044 2536 2834 256
Size: 909 879 492 298 1411
Fragments arranged by size:
1411 909 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3693 3693
Size: 3989
BsaAI yAC'GTr
Cuts at: 1342 1342
Size: 3989
BsaBI GATnn'nnATC
Cuts at: 6 1503 3540 6
Size: 1497 2037 455
Fragments arranged by size:
2037 1497 455
BsaHI Gr'CG_yC
Cuts at: 311 769 1420 1668 2370 3866 311
Size: 458 651 248 702 1496 434
Fragments arranged by size:
1496 702 651 458 434 248
BsaWI w'CCGG_w
Cuts at: 615 1178 1699 2377 2651 2798 3209 615
Size: 563 521 678 274 147 411 1395
Fragments arranged by size:
1395 678 563 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 793 793
Size: 3989
BseYI C'CCAG_C
Cuts at: 447 700 762 1414 2172 2749 3245 447
Size: 253 62 652 758 577 496 1191
Fragments arranged by size:
1191 758 652 577 496 253 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 3989
BsiEI CG_ry'CG
Cuts at: 104 1531 1577 2785 104
Size: 1427 46 1208 1308
Fragments arranged by size:
1427 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1003 1781 1971 2763 1003
Size: 778 190 792 2229
Fragments arranged by size:
2229 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 866 1003 1614 1707 1781 1971 2033 2763 866
Size: 137 611 93 74 190 62 730 2092
Fragments arranged by size:
2092 730 611 190 137 93 74 62
BspEI T'CCGG_A
Cuts at: 3209 3209
Size: 3989
BspHI T'CATG_A
Cuts at: 186 306 3165 186
Size: 120 2859 1010
Fragments arranged by size:
2859 1010 120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 1039 1253 1555 1936 509
Size: 530 214 302 381 2562
Fragments arranged by size:
2562 530 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2281 2335 3819 3984 50
Size: 2231 54 1484 165 55
Fragments arranged by size:
2231 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 396 474 729 1901 396
Size: 78 255 1172 2484
Fragments arranged by size:
2484 1172 255 78
BsrFI r'CCGG_y
Cuts at: 670 1987 2168 2377 670
Size: 1317 181 209 2282
Fragments arranged by size:
2282 1317 209 181
BssSI C'ACGA_G
Cuts at: 2260 2618 2260
Size: 358 3631
BstAPI GCAn_nnn'nTGC
Cuts at: 1366 1366
Size: 3989
BstBI TT'CG_AA
Cuts at: 2350 2350
Size: 3989
BstEII G'GTnAC_C
Cuts at: 394 394
Size: 3989
BstYI r'GATC_y
Cuts at: 1 260 749 888 1014 1481 1504 1839 2085
Size: 259 489 139 126 467 23 335 246
Cuts at: 2085 3097 3183 3195 3541 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 489 467 449 346 335 259 246
139 126 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1939 2120 2283 1939
Size: 181 163 3645
Fragments arranged by size:
3645 181 163
BtsI GCAGTG_nn'
Cuts at: 790 1232 790
Size: 442 3547
DraI TTT'AAA
Cuts at: 988 988
Size: 3989
DraIII CAC_nnn'GTG
Cuts at: 602 602
Size: 3989
DrdI GACnn_nn'nnGTC
Cuts at: 1695 2553 1695
Size: 858 3131
EaeI y'GGCC_r
Cuts at: 692 836 1574 1748 2139 2166 3511 692
Size: 144 738 174 391 27 1345 1170
Fragments arranged by size:
1345 1170 738 391 174 144 27
EagI C'GGCC_G
Cuts at: 1574 1574
Size: 3989
EarI CTCTTCn'nnn_
Cuts at: 433 739 935 2012 2222 433
Size: 306 196 1077 210 2200
Fragments arranged by size:
2200 1077 306 210 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 237 968 2197 2507 2653 3799 237
Size: 731 1229 310 146 1146 427
Fragments arranged by size:
1229 1146 731 427 310 146
EcoICRI GAG'CTC
Cuts at: 1001 1001
Size: 3989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 388 508 928 1814 2246 2993 3261 301
Size: 87 120 420 886 432 747 268 1029
Fragments arranged by size:
1029 886 747 432 420 268 120 87
EcoNI CCTnn'n_nnAGG
Cuts at: 271 271
Size: 3989
EcoO109I rG'GnC_Cy
Cuts at: 363 483 945 1128 363
Size: 120 462 183 3224
Fragments arranged by size:
3224 462 183 120
EcoRI G'AATT_C
Cuts at: 349 993 349
Size: 644 3345
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 768 2008 768
Size: 1240 2749
FspI TGC'GCA
Cuts at: 1770 1770
Size: 3989
HaeII r_GCGC'y
Cuts at: 1423 1671 2693 3672 1423
Size: 248 1022 979 1740
Fragments arranged by size:
1740 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 183 215 649 681 1831 1861 1863 1893 183
Size: 32 434 32 1150 30 2 30 2279
Fragments arranged by size:
2279 1150 434 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1028 1028
Size: 3989
KasI G'GCGC_C
Cuts at: 1419 1667 1419
Size: 248 3741
KpnI G_GTAC'C
Cuts at: 609 1009 609
Size: 400 3589
MluI A'CGCG_T
Cuts at: 3422 3422
Size: 3989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 780 992 2660 2844 780
Size: 212 1668 184 1925
Fragments arranged by size:
1925 1668 212 184
MscI TGG'CCA
Cuts at: 694 1750 694
Size: 1056 2933
MslI CAynn'nnrTG
Cuts at: 658 1978 2105 658
Size: 1320 127 2542
Fragments arranged by size:
2542 1320 127
MspA1I CmG'CkG
Cuts at: 575 586 1100 1414 1774 2787 3032 575
Size: 11 514 314 360 1013 245 1532
Fragments arranged by size:
1532 1013 514 360 314 245 11
NaeI GCC'GGC
Cuts at: 672 2170 672
Size: 1498 2491
NarI GG'CG_CC
Cuts at: 1420 1668 1420
Size: 248 3741
NgoMIV G'CCGG_C
Cuts at: 670 2168 670
Size: 1498 2491
NspI r_CATG'y
Cuts at: 663 1042 1542 2449 663
Size: 379 500 907 2203
Fragments arranged by size:
2203 907 500 379
PciI A'CATG_T
Cuts at: 659 2445 659
Size: 1786 2203
PfoI T'CCnGG_A
Cuts at: 254 254
Size: 3989
PmeI GTTT'AAAC
Cuts at: 988 988
Size: 3989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3128 3160 3128
Size: 32 3957
PpuMI rG'GwC_Cy
Cuts at: 363 483 363
Size: 120 3869
PshAI GACnn'nnGTC
Cuts at: 974 974
Size: 3989
PspXI vC'TCGA_Gb
Cuts at: 551 551
Size: 3989
PstI C_TGCA'G
Cuts at: 899 953 1036 1721 899
Size: 54 83 685 3167
Fragments arranged by size:
3167 685 83 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3989
PvuII CAG'CTG
Cuts at: 586 1414 1774 586
Size: 828 360 2801
Fragments arranged by size:
2801 828 360
RsrII CG'GwC_CG
Cuts at: 2184 2184
Size: 3989
SacI G_AGCT'C
Cuts at: 1003 1003
Size: 3989
SalI G'TCGA_C
Cuts at: 1026 1026
Size: 3989
SapI GCTCTTCn'nnn_
Cuts at: 2012 2222 2012
Size: 210 3779
SbfI CC_TGCA'GG
Cuts at: 1036 1036
Size: 3989
ScaI AGT'ACT
Cuts at: 3506 3506
Size: 3989
SfcI C'TryA_G
Cuts at: 33 895 949 1032 1717 2710 2901 33
Size: 862 54 83 685 993 191 1121
Fragments arranged by size:
1121 993 862 685 191 83 54
SfoI GGC'GCC
Cuts at: 1421 1669 1421
Size: 248 3741
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
SmaI CCC'GGG
Cuts at: 1011 1011
Size: 3989
SmlI C'TyrA_G
Cuts at: 271 551 1144 2059 2551 2813 271
Size: 280 593 915 492 262 1447
Fragments arranged by size:
1447 915 593 492 280 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3989
SphI G_CATG'C
Cuts at: 1042 1542 1042
Size: 500 3489
StyI C'CwwG_G
Cuts at: 1123 1477 1123
Size: 354 3635
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1572 1705 2164 2373 1572
Size: 133 459 209 3188
Fragments arranged by size:
3188 459 209 133
TatI w'GTAC_w
Cuts at: 641 3504 641
Size: 2863 1126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 605 637 3506 3538 605
Size: 32 2869 32 1056
Fragments arranged by size:
2869 1056 32 32
Tth111I GACn'n_nGTC
Cuts at: 1786 1786
Size: 3989
XbaI T'CTAG_A
Cuts at: 1020 1020
Size: 3989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 846 1484 846
Size: 638 3351
XhoI C'TCGA_G
Cuts at: 551 551
Size: 3989
XmaI C'CCGG_G
Cuts at: 1009 1009
Size: 3989
ZraI GAC'GTC
Cuts at: 312 312
Size: 3989
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PshAI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BbsI BfrBI BglI BmtI BpmI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI