Restriction map for
pF1KB8205
(Circular) (Six-base) MAPSORT of: pF1KB8205.seq Check: 1132 from: 1 to: 4166
pF1KB8205 4166 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1204 1204
Size: 4166
Acc65I G'GTAC_C
Cuts at: 602 968 1182 602
Size: 366 214 3586
Fragments arranged by size:
3586 366 214
AclI AA'CG_TT
Cuts at: 4006 4006
Size: 4166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1991 2423 3170 3438 1991
Size: 432 747 268 2719
Fragments arranged by size:
2719 747 432 268
AflIII A'CryG_T
Cuts at: 1518 2622 3599 1518
Size: 1104 977 2085
Fragments arranged by size:
2085 1104 977
AgeI A'CCGG_T
Cuts at: 2554 2554
Size: 4166
AleI CACnn'nnGTG
Cuts at: 550 550
Size: 4166
AlwNI CAG_nnn'CTG
Cuts at: 948 1108 1471 3038 948
Size: 160 363 1567 2076
Fragments arranged by size:
2076 1567 363 160
ApaI G_GGCC'C
Cuts at: 412 858 412
Size: 446 3720
ApaLI G'TGCA_C
Cuts at: 2936 2936
Size: 4166
ApoI r'AATT_y
Cuts at: 1170 3519 3775 1170
Size: 2349 256 1561
Fragments arranged by size:
2349 1561 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
AvaI C'yCGr_G
Cuts at: 463 1186 463
Size: 723 3443
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 586 619 586
Size: 33 4133
BamHI G'GATC_C
Cuts at: 672 701 1191 672
Size: 29 490 3647
Fragments arranged by size:
3647 490 29
BanI G'GyrC_C
Cuts at: 111 227 248 256 552 602 617 863 968
Size: 116 21 8 296 50 15 246 105
Cuts at: 968 1182 1596 1844 1879 111
Size: 214 414 248 35 2398
Fragments arranged by size:
2398 414 296 248 246 214 116 105
50 35 21 15 8
BanII G_rGCy'C
Cuts at: 170 412 449 858 1180 2210 170
Size: 242 37 409 322 1030 2126
Fragments arranged by size:
2126 1030 409 322 242 37
BbeI G_GCGC'C
Cuts at: 115 621 1600 1848 115
Size: 506 979 248 2433
Fragments arranged by size:
2433 979 506 248
BciVI GTATCCnnnnn_n'
Cuts at: 2064 2825 4166 2064
Size: 761 1341 2064
Fragments arranged by size:
2064 1341 761
BclI T'GATC_A
Cuts at: 1686 1686
Size: 4166
BglI GCCn_nnn'nGGC
Cuts at: 361 361
Size: 4166
BglII A'GATC_T
Cuts at: 1681 3718 1681
Size: 2037 2129
BlpI GC'TnA_GC
Cuts at: 1278 1278
Size: 4166
Bme1580I G_kGCm'C
Cuts at: 230 334 412 563 858 1791 1884 2940 230
Size: 104 78 151 295 933 93 1056 1456
Fragments arranged by size:
1456 1056 933 295 151 104 93 78
BmrI ACTGGGnnnn_n'
Cuts at: 919 1037 1378 1459 1794 3938 919
Size: 118 341 81 335 2144 1147
Fragments arranged by size:
2144 1147 341 335 118 81
BmtI G_CTAG'C
Cuts at: 472 472
Size: 4166
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 901 1006 526
Size: 375 105 3686
Fragments arranged by size:
3686 375 105
Bpu10I CC'TnA_GC
Cuts at: 244 244
Size: 4166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 2221 2713 3011 1342
Size: 879 492 298 2497
Fragments arranged by size:
2497 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1015 3870 1015
Size: 2855 1311
BsaAI yAC'GTr
Cuts at: 1519 1519
Size: 4166
BsaBI GATnn'nnATC
Cuts at: 6 1680 3717 6
Size: 1674 2037 455
Fragments arranged by size:
2037 1674 455
BsaHI Gr'CG_yC
Cuts at: 112 206 618 1597 1845 2547 4043 112
Size: 94 412 979 248 702 1496 235
Fragments arranged by size:
1496 979 702 412 248 235 94
BsaWI w'CCGG_w
Cuts at: 698 1355 1876 2554 2828 2975 3386 698
Size: 657 521 678 274 147 411 1478
Fragments arranged by size:
1478 678 657 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 205 523 1031 1126 166
Size: 39 318 508 95 3206
Fragments arranged by size:
3206 508 318 95 39
BseYI C'CCAG_C
Cuts at: 1591 2349 2926 3422 1591
Size: 758 577 496 2335
Fragments arranged by size:
2335 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1708 1754 2962 104
Size: 1604 46 1208 1308
Fragments arranged by size:
1604 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 887 956 1180 1958 2148 2940 887
Size: 69 224 778 190 792 2113
Fragments arranged by size:
2113 792 778 224 190 69
BsmI GAATG_Cn'
Cuts at: 378 378
Size: 4166
Bsp1286I G_dGCh'C
Cuts at: 170 230 334 412 449 563 858 887 956
Size: 60 104 78 37 114 295 29 69
Cuts at: 956 1180 1791 1884 1958 2148 2210 2940 170
Size: 224 611 93 74 190 62 730 1396
Fragments arranged by size:
1396 730 611 295 224 190 114 104
93 78 74 69 62 60 37 29
BspEI T'CCGG_A
Cuts at: 3386 3386
Size: 4166
BspHI T'CATG_A
Cuts at: 3342 3342
Size: 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 980 1216 1430 1732 2113 980
Size: 236 214 302 381 3033
Fragments arranged by size:
3033 381 302 236 214
BsrBI CCG'CTC
Cuts at: 50 2458 2512 3996 4161 50
Size: 2408 54 1484 165 55
Fragments arranged by size:
2408 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 717 2078 717
Size: 1361 2805
BsrFI r'CCGG_y
Cuts at: 114 2164 2345 2554 114
Size: 2050 181 209 1726
Fragments arranged by size:
2050 1726 209 181
BssSI C'ACGA_G
Cuts at: 2437 2795 2437
Size: 358 3808
BstAPI GCAn_nnn'nTGC
Cuts at: 1543 1543
Size: 4166
BstBI TT'CG_AA
Cuts at: 2527 2527
Size: 4166
BstEII G'GTnAC_C
Cuts at: 679 679
Size: 4166
BstXI CCAn_nnnn'nTGG
Cuts at: 579 579
Size: 4166
BstYI r'GATC_y
Cuts at: 1 672 701 1146 1191 1658 1681 2016 2262
Size: 671 29 445 45 467 23 335 246
Cuts at: 2262 3274 3360 3372 3718 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 671 467 449 445 346 335 246
86 45 29 23 12
Bsu36I CC'TnA_GG
Cuts at: 1109 1109
Size: 4166
BtgI C'CryG_G
Cuts at: 107 621 107
Size: 514 3652
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2116 2297 2460 2116
Size: 181 163 3822
Fragments arranged by size:
3822 181 163
BtsI GCAGTG_nn'
Cuts at: 606 1409 606
Size: 803 3363
DraI TTT'AAA
Cuts at: 1165 1165
Size: 4166
DrdI GACnn_nn'nnGTC
Cuts at: 1872 2730 1872
Size: 858 3308
EaeI y'GGCC_r
Cuts at: 1751 1925 2316 2343 3688 1751
Size: 174 391 27 1345 2229
Fragments arranged by size:
2229 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1751 1751
Size: 4166
EarI CTCTTCn'nnn_
Cuts at: 2189 2399 2189
Size: 210 3956
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 142 2374 2684 2830 3976 142
Size: 2232 310 146 1146 332
Fragments arranged by size:
2232 1146 332 310 146
EcoICRI GAG'CTC
Cuts at: 1178 1178
Size: 4166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 901 1006 1991 2423 3170 3438 526
Size: 375 105 985 432 747 268 1254
Fragments arranged by size:
1254 985 747 432 375 268 105
EcoNI CCTnn'n_nnAGG
Cuts at: 295 940 295
Size: 645 3521
EcoO109I rG'GnC_Cy
Cuts at: 402 513 741 1116 1305 402
Size: 111 228 375 189 3263
Fragments arranged by size:
3263 375 228 189 111
EcoRI G'AATT_C
Cuts at: 1170 1170
Size: 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 4166
FspI TGC'GCA
Cuts at: 1947 1947
Size: 4166
HaeII r_GCGC'y
Cuts at: 115 196 505 621 1600 1848 2870 3849 115
Size: 81 309 116 979 248 1022 979 432
Fragments arranged by size:
1022 979 979 432 309 248 116 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1135 1167 2008 2038 2040 2070 1135
Size: 32 841 30 2 30 3231
Fragments arranged by size:
3231 841 32 30 30 2
HincII GTy'rAC
Cuts at: 1205 1205
Size: 4166
KasI G'GCGC_C
Cuts at: 111 617 1596 1844 111
Size: 506 979 248 2433
Fragments arranged by size:
2433 979 506 248
KpnI G_GTAC'C
Cuts at: 606 972 1186 606
Size: 366 214 3586
Fragments arranged by size:
3586 366 214
MluI A'CGCG_T
Cuts at: 3599 3599
Size: 4166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2837 3021 2837
Size: 184 3982
MscI TGG'CCA
Cuts at: 1927 1927
Size: 4166
MslI CAynn'nnrTG
Cuts at: 550 2155 2282 550
Size: 1605 127 2434
Fragments arranged by size:
2434 1605 127
MspA1I CmG'CkG
Cuts at: 313 414 1277 1591 1951 2964 3209 313
Size: 101 863 314 360 1013 245 1270
Fragments arranged by size:
1270 1013 863 360 314 245 101
NaeI GCC'GGC
Cuts at: 2347 2347
Size: 4166
NarI GG'CG_CC
Cuts at: 112 618 1597 1845 112
Size: 506 979 248 2433
Fragments arranged by size:
2433 979 506 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4166
NgoMIV G'CCGG_C
Cuts at: 2345 2345
Size: 4166
NheI G'CTAG_C
Cuts at: 468 468
Size: 4166
NspI r_CATG'y
Cuts at: 921 1219 1719 2626 921
Size: 298 500 907 2461
Fragments arranged by size:
2461 907 500 298
PasI CC'CwG_GG
Cuts at: 1026 1026
Size: 4166
PciI A'CATG_T
Cuts at: 2622 2622
Size: 4166
PmeI GTTT'AAAC
Cuts at: 1165 1165
Size: 4166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 4134
PpuMI rG'GwC_Cy
Cuts at: 402 741 402
Size: 339 3827
PspOMI G'GGCC_C
Cuts at: 408 854 408
Size: 446 3720
PstI C_TGCA'G
Cuts at: 143 944 1099 1213 1898 143
Size: 801 155 114 685 2411
Fragments arranged by size:
2411 801 685 155 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4166
PvuII CAG'CTG
Cuts at: 1591 1951 1591
Size: 360 3806
RsrII CG'GwC_CG
Cuts at: 2361 2361
Size: 4166
SacI G_AGCT'C
Cuts at: 1180 1180
Size: 4166
SalI G'TCGA_C
Cuts at: 1203 1203
Size: 4166
SanDI GG'GwC_CC
Cuts at: 741 741
Size: 4166
SapI GCTCTTCn'nnn_
Cuts at: 2189 2399 2189
Size: 210 3956
SbfI CC_TGCA'GG
Cuts at: 1099 1213 1099
Size: 114 4052
ScaI AGT'ACT
Cuts at: 3683 3683
Size: 4166
SfcI C'TryA_G
Cuts at: 33 139 219 940 1078 1095 1209 1894 2887
Size: 106 80 721 138 17 114 685 993
Cuts at: 2887 3078 33
Size: 191 1121
Fragments arranged by size:
1121 993 721 685 191 138 114 106
80 17
SfoI GGC'GCC
Cuts at: 113 619 1598 1846 113
Size: 506 979 248 2433
Fragments arranged by size:
2433 979 506 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
SgrAI Cr'CCGG_yG
Cuts at: 114 114
Size: 4166
SmaI CCC'GGG
Cuts at: 1188 1188
Size: 4166
SmlI C'TyrA_G
Cuts at: 1321 2236 2728 2990 1321
Size: 915 492 262 2497
Fragments arranged by size:
2497 915 492 262
SpeI A'CTAG_T
Cuts at: 67 339 67
Size: 272 3894
SphI G_CATG'C
Cuts at: 921 1219 1719 921
Size: 298 500 3368
Fragments arranged by size:
3368 500 298
StuI AGG'CCT
Cuts at: 722 722
Size: 4166
StyI C'CwwG_G
Cuts at: 107 252 527 858 957 1139 1300 1654 107
Size: 145 275 331 99 182 161 354 2619
Fragments arranged by size:
2619 354 331 275 182 161 145 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 1749 1882 2341 2550 334
Size: 1415 133 459 209 1950
Fragments arranged by size:
1950 1415 459 209 133
TatI w'GTAC_w
Cuts at: 3681 3681
Size: 4166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 4134
Tth111I GACn'n_nGTC
Cuts at: 1963 1963
Size: 4166
XbaI T'CTAG_A
Cuts at: 1197 1197
Size: 4166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 1661 579
Size: 1082 3084
XmaI C'CCGG_G
Cuts at: 1186 1186
Size: 4166
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BbsI BbvCI BcgI BfrBI BmgBI BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI