Restriction map for
pF1KB8199
(Circular) (Six-base) MAPSORT of: pF1KB8199.seq Check: 5339 from: 1 to: 3731
pF1KB8199 3731 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 769 769
Size: 3731
Acc65I G'GTAC_C
Cuts at: 747 747
Size: 3731
AclI AA'CG_TT
Cuts at: 725 3571 725
Size: 2846 885
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 1556 1988 2735 3003 355
Size: 1201 432 747 268 1083
Fragments arranged by size:
1201 1083 747 432 268
AflIII A'CryG_T
Cuts at: 1083 2187 3164 1083
Size: 1104 977 1650
Fragments arranged by size:
1650 1104 977
AgeI A'CCGG_T
Cuts at: 2119 2119
Size: 3731
AlwNI CAG_nnn'CTG
Cuts at: 1036 2603 1036
Size: 1567 2164
ApaLI G'TGCA_C
Cuts at: 2501 2501
Size: 3731
ApoI r'AATT_y
Cuts at: 693 735 3084 3340 693
Size: 42 2349 256 1084
Fragments arranged by size:
2349 1084 256 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 3731
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
AvaI C'yCGr_G
Cuts at: 143 221 751 143
Size: 78 530 3123
Fragments arranged by size:
3123 530 78
BamHI G'GATC_C
Cuts at: 756 756
Size: 3731
BanI G'GyrC_C
Cuts at: 747 1161 1409 1444 747
Size: 414 248 35 3034
Fragments arranged by size:
3034 414 248 35
BanII G_rGCy'C
Cuts at: 745 1775 745
Size: 1030 2701
BbeI G_GCGC'C
Cuts at: 1165 1413 1165
Size: 248 3483
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3697
BciVI GTATCCnnnnn_n'
Cuts at: 1629 2390 3731 1629
Size: 761 1341 1629
Fragments arranged by size:
1629 1341 761
BclI T'GATC_A
Cuts at: 1251 1251
Size: 3731
BglI GCCn_nnn'nGGC
Cuts at: 179 179
Size: 3731
BglII A'GATC_T
Cuts at: 681 1246 3283 681
Size: 565 2037 1129
Fragments arranged by size:
2037 1129 565
BlpI GC'TnA_GC
Cuts at: 843 843
Size: 3731
Bme1580I G_kGCm'C
Cuts at: 1356 1449 2505 1356
Size: 93 1056 2582
Fragments arranged by size:
2582 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 943 1024 1359 3503 943
Size: 81 335 2144 1171
Fragments arranged by size:
2144 1171 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 3731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 674
Size: 3731
Bpu10I CC'TnA_GC
Cuts at: 131 131
Size: 3731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1786 2278 2576 907
Size: 879 492 298 2062
Fragments arranged by size:
2062 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3435 3435
Size: 3731
BsaAI yAC'GTr
Cuts at: 1084 1084
Size: 3731
BsaBI GATnn'nnATC
Cuts at: 6 1245 3282 6
Size: 1239 2037 455
Fragments arranged by size:
2037 1239 455
BsaHI Gr'CG_yC
Cuts at: 1162 1410 2112 3608 1162
Size: 248 702 1496 1285
Fragments arranged by size:
1496 1285 702 248
BsaWI w'CCGG_w
Cuts at: 920 1441 2119 2393 2540 2951 920
Size: 521 678 274 147 411 1700
Fragments arranged by size:
1700 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 550 1156 1914 2491 2987 550
Size: 606 758 577 496 1294
Fragments arranged by size:
1294 758 606 577 496
BsiEI CG_ry'CG
Cuts at: 104 1273 1319 2527 104
Size: 1169 46 1208 1308
Fragments arranged by size:
1308 1208 1169 46
BsiHKAI G_wGCw'C
Cuts at: 745 1523 1713 2505 745
Size: 778 190 792 1971
Fragments arranged by size:
1971 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 517 517
Size: 3731
Bsp1286I G_dGCh'C
Cuts at: 745 1356 1449 1523 1713 1775 2505 745
Size: 611 93 74 190 62 730 1971
Fragments arranged by size:
1971 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2951 2951
Size: 3731
BspHI T'CATG_A
Cuts at: 254 2907 254
Size: 2653 1078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 781 995 1297 1678 781
Size: 214 302 381 2834
Fragments arranged by size:
2834 381 302 214
BsrBI CCG'CTC
Cuts at: 50 130 2023 2077 3561 3726 50
Size: 80 1893 54 1484 165 55
Fragments arranged by size:
1893 1484 165 80 55 54
BsrDI GCAATG_nn'
Cuts at: 1643 1643
Size: 3731
BsrFI r'CCGG_y
Cuts at: 1729 1910 2119 1729
Size: 181 209 3341
Fragments arranged by size:
3341 209 181
BssSI C'ACGA_G
Cuts at: 2002 2360 2002
Size: 358 3373
BstAPI GCAn_nnn'nTGC
Cuts at: 646 1108 646
Size: 462 3269
BstBI TT'CG_AA
Cuts at: 2092 2092
Size: 3731
BstYI r'GATC_y
Cuts at: 1 681 756 1223 1246 1581 1827 2839 2925
Size: 680 75 467 23 335 246 1012 86
Cuts at: 2925 2937 3283 1
Size: 12 346 449
Fragments arranged by size:
1012 680 467 449 346 335 246 86
75 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1681 1862 2025 1681
Size: 181 163 3387
Fragments arranged by size:
3387 181 163
BtsI GCAGTG_nn'
Cuts at: 974 974
Size: 3731
DraI TTT'AAA
Cuts at: 730 730
Size: 3731
DrdI GACnn_nn'nnGTC
Cuts at: 1437 2295 1437
Size: 858 2873
EaeI y'GGCC_r
Cuts at: 602 1316 1490 1881 1908 3253 602
Size: 714 174 391 27 1345 1080
Fragments arranged by size:
1345 1080 714 391 174 27
EagI C'GGCC_G
Cuts at: 1316 1316
Size: 3731
EarI CTCTTCn'nnn_
Cuts at: 413 1754 1964 413
Size: 1341 210 2180
Fragments arranged by size:
2180 1341 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 230 1939 2249 2395 3541 230
Size: 1709 310 146 1146 420
Fragments arranged by size:
1709 1146 420 310 146
EcoICRI GAG'CTC
Cuts at: 743 743
Size: 3731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 674 1556 1988 2735 3003 355
Size: 319 882 432 747 268 1083
Fragments arranged by size:
1083 882 747 432 319 268
EcoO109I rG'GnC_Cy
Cuts at: 870 870
Size: 3731
EcoRI G'AATT_C
Cuts at: 735 735
Size: 3731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 661 1750 661
Size: 1089 2642
FspI TGC'GCA
Cuts at: 295 1512 295
Size: 1217 2514
FspAI rTGC'GCAy
Cuts at: 295 295
Size: 3731
HaeII r_GCGC'y
Cuts at: 1165 1413 2435 3414 1165
Size: 248 1022 979 1482
Fragments arranged by size:
1482 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 180 212 1573 1603 1605 1635 180
Size: 32 1361 30 2 30 2276
Fragments arranged by size:
2276 1361 32 30 30 2
HincII GTy'rAC
Cuts at: 770 770
Size: 3731
KasI G'GCGC_C
Cuts at: 1161 1409 1161
Size: 248 3483
KpnI G_GTAC'C
Cuts at: 751 751
Size: 3731
MluI A'CGCG_T
Cuts at: 3164 3164
Size: 3731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2402 2586 2402
Size: 184 3547
MscI TGG'CCA
Cuts at: 1492 1492
Size: 3731
MslI CAynn'nnrTG
Cuts at: 1720 1847 1720
Size: 127 3604
MspA1I CmG'CkG
Cuts at: 534 842 1156 1516 2529 2774 534
Size: 308 314 360 1013 245 1491
Fragments arranged by size:
1491 1013 360 314 308 245
NaeI GCC'GGC
Cuts at: 1912 1912
Size: 3731
NarI GG'CG_CC
Cuts at: 1162 1410 1162
Size: 248 3483
NgoMIV G'CCGG_C
Cuts at: 1910 1910
Size: 3731
NspI r_CATG'y
Cuts at: 784 1284 2191 784
Size: 500 907 2324
Fragments arranged by size:
2324 907 500
PasI CC'CwG_GG
Cuts at: 175 175
Size: 3731
PciI A'CATG_T
Cuts at: 2187 2187
Size: 3731
PmeI GTTT'AAAC
Cuts at: 730 730
Size: 3731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 2902 2870
Size: 32 3699
PstI C_TGCA'G
Cuts at: 284 778 1463 284
Size: 494 685 2552
Fragments arranged by size:
2552 685 494
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3731
PvuII CAG'CTG
Cuts at: 534 1156 1516 534
Size: 622 360 2749
Fragments arranged by size:
2749 622 360
RsrII CG'GwC_CG
Cuts at: 1926 1926
Size: 3731
SacI G_AGCT'C
Cuts at: 745 745
Size: 3731
SalI G'TCGA_C
Cuts at: 768 768
Size: 3731
SapI GCTCTTCn'nnn_
Cuts at: 1754 1964 1754
Size: 210 3521
SbfI CC_TGCA'GG
Cuts at: 778 778
Size: 3731
ScaI AGT'ACT
Cuts at: 3248 3248
Size: 3731
SfcI C'TryA_G
Cuts at: 33 280 774 1459 2452 2643 33
Size: 247 494 685 993 191 1121
Fragments arranged by size:
1121 993 685 494 247 191
SfoI GGC'GCC
Cuts at: 1163 1411 1163
Size: 248 3483
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
SmaI CCC'GGG
Cuts at: 753 753
Size: 3731
SmlI C'TyrA_G
Cuts at: 886 1801 2293 2555 886
Size: 915 492 262 2062
Fragments arranged by size:
2062 915 492 262
SpeI A'CTAG_T
Cuts at: 67 586 67
Size: 519 3212
SphI G_CATG'C
Cuts at: 784 1284 784
Size: 500 3231
StyI C'CwwG_G
Cuts at: 441 865 1219 441
Size: 424 354 2953
Fragments arranged by size:
2953 424 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1314 1447 1906 2115 1314
Size: 133 459 209 2930
Fragments arranged by size:
2930 459 209 133
TatI w'GTAC_w
Cuts at: 3246 3246
Size: 3731
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 3699
Tth111I GACn'n_nGTC
Cuts at: 192 1528 192
Size: 1336 2395
XbaI T'CTAG_A
Cuts at: 762 762
Size: 3731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 624 1226 624
Size: 602 3129
XmaI C'CCGG_G
Cuts at: 751 751
Size: 3731
XmnI GAAnn'nnTTC
Cuts at: 499 499
Size: 3731
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI
BmtI BsaXI BseRI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI