Restriction map for
pF1KB8196
(Circular) (Six-base) MAPSORT of: pF1KB8196.seq Check: 6627 from: 1 to: 4487
pF1KB8196 4487 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 591 591
Size: 4487
AatII G_ACGT'C
Cuts at: 229 328 229
Size: 99 4388
AccI GT'mk_AC
Cuts at: 1525 1525
Size: 4487
Acc65I G'GTAC_C
Cuts at: 1503 1503
Size: 4487
AclI AA'CG_TT
Cuts at: 4327 4327
Size: 4487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1400 2312 2744 3491 3759 1400
Size: 912 432 747 268 2128
Fragments arranged by size:
2128 912 747 432 268
AflIII A'CryG_T
Cuts at: 749 1839 2943 3920 749
Size: 1090 1104 977 1316
Fragments arranged by size:
1316 1104 1090 977
AgeI A'CCGG_T
Cuts at: 2875 2875
Size: 4487
AhdI GACnn_n'nnGTC
Cuts at: 272 338 1214 272
Size: 66 876 3545
Fragments arranged by size:
3545 876 66
AleI CACnn'nnGTG
Cuts at: 772 772
Size: 4487
AlwNI CAG_nnn'CTG
Cuts at: 278 583 1792 3359 278
Size: 305 1209 1567 1406
Fragments arranged by size:
1567 1406 1209 305
ApaI G_GGCC'C
Cuts at: 219 252 285 219
Size: 33 33 4421
Fragments arranged by size:
4421 33 33
ApaLI G'TGCA_C
Cuts at: 3257 3257
Size: 4487
ApoI r'AATT_y
Cuts at: 1152 1491 3840 4096 1152
Size: 339 2349 256 1543
Fragments arranged by size:
2349 1543 339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4487
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
AvaI C'yCGr_G
Cuts at: 245 251 311 336 344 383 1507 245
Size: 6 60 25 8 39 1124 3225
Fragments arranged by size:
3225 1124 60 39 25 8 6
BamHI G'GATC_C
Cuts at: 777 1512 777
Size: 735 3752
BanI G'GyrC_C
Cuts at: 614 1503 1917 2165 2200 614
Size: 889 414 248 35 2901
Fragments arranged by size:
2901 889 414 248 35
BanII G_rGCy'C
Cuts at: 219 252 285 683 1306 1501 2531 219
Size: 33 33 398 623 195 1030 2175
Fragments arranged by size:
2175 1030 623 398 195 33 33
BbeI G_GCGC'C
Cuts at: 1921 2169 1921
Size: 248 4239
BbsI GAAGACnn'nnnn_
Cuts at: 580 1111 580
Size: 531 3956
BbvCI CC'TCA_GC
Cuts at: 802 802
Size: 4487
BciVI GTATCCnnnnn_n'
Cuts at: 2385 3146 4487 2385
Size: 761 1341 2385
Fragments arranged by size:
2385 1341 761
BclI T'GATC_A
Cuts at: 2007 2007
Size: 4487
BglII A'GATC_T
Cuts at: 2002 4039 2002
Size: 2037 2450
BlpI GC'TnA_GC
Cuts at: 739 849 941 1599 739
Size: 110 92 658 3627
Fragments arranged by size:
3627 658 110 92
Bme1580I G_kGCm'C
Cuts at: 219 252 285 351 2112 2205 3261 219
Size: 33 33 66 1761 93 1056 1445
Fragments arranged by size:
1761 1445 1056 93 66 33 33
BmrI ACTGGGnnnn_n'
Cuts at: 266 1407 1699 1780 2115 4259 266
Size: 1141 292 81 335 2144 494
Fragments arranged by size:
2144 1141 494 335 292 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 1046 162
Size: 884 3603
Bpu10I CC'TnA_GC
Cuts at: 654 802 654
Size: 148 4339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1663 2542 3034 3332 1663
Size: 879 492 298 2818
Fragments arranged by size:
2818 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 183 950 4191 183
Size: 767 3241 479
Fragments arranged by size:
3241 767 479
BsaAI yAC'GTr
Cuts at: 752 1840 752
Size: 1088 3399
BsaBI GATnn'nnATC
Cuts at: 6 2001 4038 6
Size: 1995 2037 455
Fragments arranged by size:
2037 1995 455
BsaHI Gr'CG_yC
Cuts at: 226 325 1918 2166 2868 4364 226
Size: 99 1593 248 702 1496 349
Fragments arranged by size:
1593 1496 702 349 248 99
BsaWI w'CCGG_w
Cuts at: 1162 1676 2197 2875 3149 3296 3707 1162
Size: 514 521 678 274 147 411 1942
Fragments arranged by size:
1942 678 521 514 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 180 210 180
Size: 30 4457
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 201 1149 201
Size: 948 3539
BseYI C'CCAG_C
Cuts at: 179 438 445 1178 1219 1912 2670 3247 3743
Size: 259 7 733 41 693 758 577 496
Cuts at: 3743 179
Size: 923
Fragments arranged by size:
923 758 733 693 577 496 259 41
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 1295 1444 155
Size: 1140 149 3198
Fragments arranged by size:
3198 1140 149
BsiEI CG_ry'CG
Cuts at: 104 497 2029 2075 3283 104
Size: 393 1532 46 1208 1308
Fragments arranged by size:
1532 1308 1208 393 46
BsiHKAI G_wGCw'C
Cuts at: 1501 2279 2469 3261 1501
Size: 778 190 792 2727
Fragments arranged by size:
2727 792 778 190
BsmI GAATG_Cn'
Cuts at: 173 173
Size: 4487
Bsp1286I G_dGCh'C
Cuts at: 219 252 285 351 683 1306 1501 2112 2205
Size: 33 33 66 332 623 195 611 93
Cuts at: 2205 2279 2469 2531 3261 219
Size: 74 190 62 730 1445
Fragments arranged by size:
1445 730 623 611 332 195 190 93
74 66 62 33 33
BspEI T'CCGG_A
Cuts at: 1162 3707 1162
Size: 2545 1942
BspHI T'CATG_A
Cuts at: 3663 3663
Size: 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 591 1537 1751 2053 2434 591
Size: 946 214 302 381 2644
Fragments arranged by size:
2644 946 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2779 2833 4317 4482 50
Size: 2729 54 1484 165 55
Fragments arranged by size:
2729 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2399 2399
Size: 4487
BsrFI r'CCGG_y
Cuts at: 611 2485 2666 2875 611
Size: 1874 181 209 2223
Fragments arranged by size:
2223 1874 209 181
BssHII G'CGCG_C
Cuts at: 417 417
Size: 4487
BssSI C'ACGA_G
Cuts at: 2758 3116 2758
Size: 358 4129
BstAPI GCAn_nnn'nTGC
Cuts at: 1864 1864
Size: 4487
BstBI TT'CG_AA
Cuts at: 1401 2848 1401
Size: 1447 3040
BstEII G'GTnAC_C
Cuts at: 363 363
Size: 4487
BstXI CCAn_nnnn'nTGG
Cuts at: 960 960
Size: 4487
BstYI r'GATC_y
Cuts at: 1 602 777 1427 1512 1979 2002 2337 2583
Size: 601 175 650 85 467 23 335 246
Cuts at: 2583 3595 3681 3693 4039 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 650 601 467 449 346 335 246
175 86 85 23 12
Bsu36I CC'TnA_GG
Cuts at: 1469 1469
Size: 4487
BtgI C'CryG_G
Cuts at: 107 587 761 782 884 107
Size: 480 174 21 102 3710
Fragments arranged by size:
3710 480 174 102 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 540 777 2437 2618 2781 540
Size: 237 1660 181 163 2246
Fragments arranged by size:
2246 1660 237 181 163
BtsI GCAGTG_nn'
Cuts at: 461 901 1427 1730 461
Size: 440 526 303 3218
Fragments arranged by size:
3218 526 440 303
DraI TTT'AAA
Cuts at: 1486 1486
Size: 4487
DrdI GACnn_nn'nnGTC
Cuts at: 2193 3051 2193
Size: 858 3629
EaeI y'GGCC_r
Cuts at: 494 1349 2072 2246 2637 2664 4009 494
Size: 855 723 174 391 27 1345 972
Fragments arranged by size:
1345 972 855 723 391 174 27
EagI C'GGCC_G
Cuts at: 494 2072 494
Size: 1578 2909
EarI CTCTTCn'nnn_
Cuts at: 2510 2720 2510
Size: 210 4277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2695 3005 3151 4297 2695
Size: 310 146 1146 2885
Fragments arranged by size:
2885 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1499 1499
Size: 4487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 1046 1400 2312 2744 3491 3759 162
Size: 884 354 912 432 747 268 890
Fragments arranged by size:
912 890 884 747 432 354 268
EcoO109I rG'GnC_Cy
Cuts at: 216 266 332 340 380 487 636 1030 1175
Size: 50 66 8 40 107 149 394 145
Cuts at: 1175 1626 216
Size: 451 3077
Fragments arranged by size:
3077 451 394 149 145 107 66 50
40 8
EcoRI G'AATT_C
Cuts at: 1152 1491 1152
Size: 339 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4487
FspI TGC'GCA
Cuts at: 2268 2268
Size: 4487
HaeII r_GCGC'y
Cuts at: 439 446 1188 1921 2169 3191 4170 439
Size: 7 742 733 248 1022 979 756
Fragments arranged by size:
1022 979 756 742 733 248 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 258 290 324 356 1200 1232 2329 2359 2361
Size: 32 34 32 844 32 1097 30 2
Cuts at: 2361 2391 258
Size: 30 2354
Fragments arranged by size:
2354 1097 844 34 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1041 1526 1041
Size: 485 4002
HindIII A'AGCT_T
Cuts at: 1254 1254
Size: 4487
HpaI GTT'AAC
Cuts at: 1041 1041
Size: 4487
KasI G'GCGC_C
Cuts at: 1917 2165 1917
Size: 248 4239
KpnI G_GTAC'C
Cuts at: 1507 1507
Size: 4487
MluI A'CGCG_T
Cuts at: 3920 3920
Size: 4487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3158 3342 3158
Size: 184 4303
MscI TGG'CCA
Cuts at: 1351 2248 1351
Size: 897 3590
MslI CAynn'nnrTG
Cuts at: 772 1195 2476 2603 772
Size: 423 1281 127 2656
Fragments arranged by size:
2656 1281 423 127
MspA1I CmG'CkG
Cuts at: 772 853 1087 1182 1598 1912 2272 3285 3530
Size: 81 234 95 416 314 360 1013 245
Cuts at: 3530 772
Size: 1729
Fragments arranged by size:
1729 1013 416 360 314 245 234 95
81
NaeI GCC'GGC
Cuts at: 2668 2668
Size: 4487
NarI GG'CG_CC
Cuts at: 1918 2166 1918
Size: 248 4239
NcoI C'CATG_G
Cuts at: 107 884 107
Size: 777 3710
NgoMIV G'CCGG_C
Cuts at: 2666 2666
Size: 4487
NspI r_CATG'y
Cuts at: 320 1540 2040 2947 320
Size: 1220 500 907 1860
Fragments arranged by size:
1860 1220 907 500
PciI A'CATG_T
Cuts at: 2943 2943
Size: 4487
PflMI CCAn_nnn'nTGG
Cuts at: 278 705 278
Size: 427 4060
PfoI T'CCnGG_A
Cuts at: 605 605
Size: 4487
PmeI GTTT'AAAC
Cuts at: 1486 1486
Size: 4487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4455
PpuMI rG'GwC_Cy
Cuts at: 266 332 340 380 487 636 1030 1175 266
Size: 66 8 40 107 149 394 145 3578
Fragments arranged by size:
3578 394 149 145 107 66 40 8
PspOMI G'GGCC_C
Cuts at: 215 248 281 215
Size: 33 33 4421
Fragments arranged by size:
4421 33 33
PstI C_TGCA'G
Cuts at: 908 1534 2219 908
Size: 626 685 3176
Fragments arranged by size:
3176 685 626
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4487
PvuII CAG'CTG
Cuts at: 772 853 1087 1912 2272 772
Size: 81 234 825 360 2987
Fragments arranged by size:
2987 825 360 234 81
RsrII CG'GwC_CG
Cuts at: 2682 2682
Size: 4487
SacI G_AGCT'C
Cuts at: 1501 1501
Size: 4487
SalI G'TCGA_C
Cuts at: 1524 1524
Size: 4487
SanDI GG'GwC_CC
Cuts at: 266 332 340 1175 266
Size: 66 8 835 3578
Fragments arranged by size:
3578 835 66 8
SapI GCTCTTCn'nnn_
Cuts at: 2510 2720 2510
Size: 210 4277
SbfI CC_TGCA'GG
Cuts at: 1534 1534
Size: 4487
ScaI AGT'ACT
Cuts at: 4004 4004
Size: 4487
SfcI C'TryA_G
Cuts at: 33 904 1237 1530 2215 3208 3399 33
Size: 871 333 293 685 993 191 1121
Fragments arranged by size:
1121 993 871 685 333 293 191
SfoI GGC'GCC
Cuts at: 1919 2167 1919
Size: 248 4239
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
SmaI CCC'GGG
Cuts at: 247 338 346 385 1509 247
Size: 91 8 39 1124 3225
Fragments arranged by size:
3225 1124 91 39 8
SmlI C'TyrA_G
Cuts at: 1642 2557 3049 3311 1642
Size: 915 492 262 2818
Fragments arranged by size:
2818 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4487
SphI G_CATG'C
Cuts at: 320 1540 2040 320
Size: 1220 500 2767
Fragments arranged by size:
2767 1220 500
StuI AGG'CCT
Cuts at: 391 391
Size: 4487
StyI C'CwwG_G
Cuts at: 107 884 1621 1975 107
Size: 777 737 354 2619
Fragments arranged by size:
2619 777 737 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2070 2203 2662 2871 2070
Size: 133 459 209 3686
Fragments arranged by size:
3686 459 209 133
TatI w'GTAC_w
Cuts at: 545 1278 4002 545
Size: 733 2724 1030
Fragments arranged by size:
2724 1030 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 650 682 4004 4036 650
Size: 32 3322 32 1101
Fragments arranged by size:
3322 1101 32 32
Tth111I GACn'n_nGTC
Cuts at: 2284 2284
Size: 4487
XbaI T'CTAG_A
Cuts at: 1518 1518
Size: 4487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 699 837 1982 699
Size: 138 1145 3204
Fragments arranged by size:
3204 1145 138
XmaI C'CCGG_G
Cuts at: 245 336 344 383 1507 245
Size: 91 8 39 1124 3225
Fragments arranged by size:
3225 1124 91 39 8
XmnI GAAnn'nnTTC
Cuts at: 411 989 1397 411
Size: 578 408 3501
Fragments arranged by size:
3501 578 408
ZraI GAC'GTC
Cuts at: 227 326 227
Size: 99 4388
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI AscI AvrII BaeI BcgI
BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI