Restriction map for
pF1KB8195
(Circular) (Six-base) MAPSORT of: pF1KB8195.seq Check: 7869 from: 1 to: 4052
pF1KB8195 4052 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1090 1090
Size: 4052
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1877 2309 3056 3324 739
Size: 1138 432 747 268 1467
Fragments arranged by size:
1467 1138 747 432 268
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 334 334
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 150 222 279 330 1357 2924 150
Size: 72 57 51 1027 1567 1278
Fragments arranged by size:
1567 1278 1027 72 57 51
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 1056 3405 3661 1056
Size: 2349 256 1447
Fragments arranged by size:
2349 1447 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 892 1068 1482 1730 1765 892
Size: 176 414 248 35 3179
Fragments arranged by size:
3179 414 248 176 35
BanII G_rGCy'C
Cuts at: 340 467 1066 2096 340
Size: 127 599 1030 2296
Fragments arranged by size:
2296 1030 599 127
BbeI G_GCGC'C
Cuts at: 1486 1734 1486
Size: 248 3804
BbsI GAAGACnn'nnnn_
Cuts at: 257 257
Size: 4052
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 1677 1770 2826 1677
Size: 93 1056 2903
Fragments arranged by size:
2903 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 384 2419 384
Size: 2035 2017
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 600 685 244
Size: 356 85 3611
Fragments arranged by size:
3611 356 85
Bpu10I CC'TnA_GC
Cuts at: 191 991 191
Size: 800 3252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1228 2107 2599 2897 688
Size: 540 879 492 298 1843
Fragments arranged by size:
1843 879 540 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3756 3756
Size: 4052
BsaAI yAC'GTr
Cuts at: 633 1405 633
Size: 772 3280
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 1015 1483 1731 2433 3929 1015
Size: 468 248 702 1496 1138
Fragments arranged by size:
1496 1138 702 468 248
BsaWI w'CCGG_w
Cuts at: 1004 1241 1762 2440 2714 2861 3272 1004
Size: 237 521 678 274 147 411 1784
Fragments arranged by size:
1784 678 521 411 274 237 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 217
Size: 30 4022
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 348 376 348
Size: 28 4024
BseYI C'CCAG_C
Cuts at: 471 736 950 1018 1477 2235 2812 3308 471
Size: 265 214 68 459 758 577 496 1215
Fragments arranged by size:
1215 758 577 496 459 265 214 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 562
Size: 4052
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 467 1066 1844 2034 2826 467
Size: 599 778 190 792 1693
Fragments arranged by size:
1693 792 778 599 190
Bsp1286I G_dGCh'C
Cuts at: 340 467 1066 1677 1770 1844 2034 2096 2826
Size: 127 599 611 93 74 190 62 730
Cuts at: 2826 340
Size: 1566
Fragments arranged by size:
1566 730 611 599 190 127 93 74
62
BspEI T'CCGG_A
Cuts at: 1004 3272 1004
Size: 2268 1784
BspHI T'CATG_A
Cuts at: 797 3228 797
Size: 2431 1621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1102 1316 1618 1999 1102
Size: 214 302 381 3155
Fragments arranged by size:
3155 381 302 214
BsrBI CCG'CTC
Cuts at: 50 555 2344 2398 3882 4047 50
Size: 505 1789 54 1484 165 55
Fragments arranged by size:
1789 1484 505 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1964 1964
Size: 4052
BsrFI r'CCGG_y
Cuts at: 772 2050 2231 2440 772
Size: 1278 181 209 2384
Fragments arranged by size:
2384 1278 209 181
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 811 2413 811
Size: 1602 2450
BstYI r'GATC_y
Cuts at: 1 1001 1077 1544 1567 1902 2148 3160 3246
Size: 1000 76 467 23 335 246 1012 86
Cuts at: 3246 3258 3604 1
Size: 12 346 449
Fragments arranged by size:
1012 1000 467 449 346 335 246 86
76 23 12
Bsu36I CC'TnA_GG
Cuts at: 331 331
Size: 4052
BtgI C'CryG_G
Cuts at: 107 305 641 743 107
Size: 198 336 102 3416
Fragments arranged by size:
3416 336 198 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 753 2002 2183 2346 753
Size: 1249 181 163 2459
Fragments arranged by size:
2459 1249 181 163
BtsI GCAGTG_nn'
Cuts at: 1295 1295
Size: 4052
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 693 1758 2616 693
Size: 1065 858 2129
Fragments arranged by size:
2129 1065 858
EaeI y'GGCC_r
Cuts at: 917 1637 1811 2202 2229 3574 917
Size: 720 174 391 27 1345 1395
Fragments arranged by size:
1395 1345 720 391 174 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 465 1064 465
Size: 599 3453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 600 685 739 1877 2309 3056 3324 244
Size: 356 85 54 1138 432 747 268 972
Fragments arranged by size:
1138 972 747 432 356 268 85 54
EcoNI CCTnn'n_nnAGG
Cuts at: 594 928 594
Size: 334 3718
EcoO109I rG'GnC_Cy
Cuts at: 174 327 732 1191 174
Size: 153 405 459 3035
Fragments arranged by size:
3035 459 405 153
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 4052
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 126 387 613 1025 1486 1734 2756 3735 126
Size: 261 226 412 461 248 1022 979 443
Fragments arranged by size:
1022 979 461 443 412 261 248 226
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 993 1025 1894 1924 1926 1956 993
Size: 32 869 30 2 30 3089
Fragments arranged by size:
3089 869 32 30 30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
KasI G'GCGC_C
Cuts at: 1482 1730 1482
Size: 248 3804
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 722 991 2723 2907 722
Size: 269 1732 184 1867
Fragments arranged by size:
1867 1732 269 184
MscI TGG'CCA
Cuts at: 919 1813 919
Size: 894 3158
MslI CAynn'nnrTG
Cuts at: 802 2041 2168 802
Size: 1239 127 2686
Fragments arranged by size:
2686 1239 127
MspA1I CmG'CkG
Cuts at: 1163 1477 1837 2850 3095 1163
Size: 314 360 1013 245 2120
Fragments arranged by size:
2120 1013 360 314 245
NaeI GCC'GGC
Cuts at: 774 2233 774
Size: 1459 2593
NarI GG'CG_CC
Cuts at: 1483 1731 1483
Size: 248 3804
NcoI C'CATG_G
Cuts at: 107 107
Size: 4052
NgoMIV G'CCGG_C
Cuts at: 772 2231 772
Size: 1459 2593
NspI r_CATG'y
Cuts at: 1105 1605 2512 1105
Size: 500 907 2645
Fragments arranged by size:
2645 907 500
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PflMI CCAn_nnn'nTGG
Cuts at: 222 222
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PmlI CAC'GTG
Cuts at: 633 633
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PpuMI rG'GwC_Cy
Cuts at: 327 327
Size: 4052
PstI C_TGCA'G
Cuts at: 1099 1784 1099
Size: 685 3367
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 1477 1837 1477
Size: 360 3692
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 467 1066 467
Size: 599 3453
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 510 579 1095 1780 2773 2964 33
Size: 477 69 516 685 993 191 1121
Fragments arranged by size:
1121 993 685 516 477 191 69
SfoI GGC'GCC
Cuts at: 1484 1732 1484
Size: 248 3804
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 703 1207 2122 2614 2876 703
Size: 504 915 492 262 1879
Fragments arranged by size:
1879 915 504 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StuI AGG'CCT
Cuts at: 589 589
Size: 4052
StyI C'CwwG_G
Cuts at: 107 878 1186 1540 107
Size: 771 308 354 2619
Fragments arranged by size:
2619 771 354 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1635 1768 2227 2436 1635
Size: 133 459 209 3251
Fragments arranged by size:
3251 459 209 133
TatI w'GTAC_w
Cuts at: 3567 3567
Size: 4052
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 219 750 1547 219
Size: 531 797 2724
Fragments arranged by size:
2724 797 531
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI