Restriction map for
pF1KB8194
(Circular) (Six-base) MAPSORT of: pF1KB8194.seq Check: 6917 from: 1 to: 4754
pF1KB8194 4754 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 437 1792 437
Size: 1355 3399
Acc65I G'GTAC_C
Cuts at: 1770 1770
Size: 4754
AclI AA'CG_TT
Cuts at: 625 4594 625
Size: 3969 785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2579 3011 3758 4026 2579
Size: 432 747 268 3307
Fragments arranged by size:
3307 747 432 268
AflIII A'CryG_T
Cuts at: 809 2106 3210 4187 809
Size: 1297 1104 977 1376
Fragments arranged by size:
1376 1297 1104 977
AgeI A'CCGG_T
Cuts at: 3142 3142
Size: 4754
AlwNI CAG_nnn'CTG
Cuts at: 2059 3626 2059
Size: 1567 3187
ApaLI G'TGCA_C
Cuts at: 3524 3524
Size: 4754
ApoI r'AATT_y
Cuts at: 669 1337 1579 1758 4107 4363 669
Size: 668 242 179 2349 256 1060
Fragments arranged by size:
2349 1060 668 256 242 179
AseI AT'TA_AT
Cuts at: 20 20
Size: 4754
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4754
AvaI C'yCGr_G
Cuts at: 1187 1774 1187
Size: 587 4167
BamHI G'GATC_C
Cuts at: 1160 1569 1779 1160
Size: 409 210 4135
Fragments arranged by size:
4135 409 210
BanI G'GyrC_C
Cuts at: 1681 1770 2184 2432 2467 1681
Size: 89 414 248 35 3968
Fragments arranged by size:
3968 414 248 89 35
BanII G_rGCy'C
Cuts at: 1768 2798 1768
Size: 1030 3724
BbeI G_GCGC'C
Cuts at: 2188 2436 2188
Size: 248 4506
BbsI GAAGACnn'nnnn_
Cuts at: 1433 1433
Size: 4754
BciVI GTATCCnnnnn_n'
Cuts at: 2652 3413 4754 2652
Size: 761 1341 2652
Fragments arranged by size:
2652 1341 761
BclI T'GATC_A
Cuts at: 2274 2274
Size: 4754
BglII A'GATC_T
Cuts at: 129 2269 4306 129
Size: 2140 2037 577
Fragments arranged by size:
2140 2037 577
BlpI GC'TnA_GC
Cuts at: 143 1866 143
Size: 1723 3031
Bme1580I G_kGCm'C
Cuts at: 2379 2472 3528 2379
Size: 93 1056 3605
Fragments arranged by size:
3605 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 474 1966 2047 2382 4526 474
Size: 1492 81 335 2144 702
Fragments arranged by size:
2144 1492 702 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 522 1145 3121 522
Size: 623 1976 2155
Fragments arranged by size:
2155 1976 623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 856
Size: 4754
Bpu10I CC'TnA_GC
Cuts at: 1233 1233
Size: 4754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1930 2809 3301 3599 1930
Size: 879 492 298 3085
Fragments arranged by size:
3085 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4458 4458
Size: 4754
BsaAI yAC'GTr
Cuts at: 2107 2107
Size: 4754
BsaBI GATnn'nnATC
Cuts at: 6 2268 4305 6
Size: 2262 2037 455
Fragments arranged by size:
2262 2037 455
BsaHI Gr'CG_yC
Cuts at: 2185 2433 3135 4631 2185
Size: 248 702 1496 2308
Fragments arranged by size:
2308 1496 702 248
BsaWI w'CCGG_w
Cuts at: 937 1943 2464 3142 3416 3563 3974 937
Size: 1006 521 678 274 147 411 1717
Fragments arranged by size:
1717 1006 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 293 293
Size: 4754
BseYI C'CCAG_C
Cuts at: 266 1403 2179 2937 3514 4010 266
Size: 1137 776 758 577 496 1010
Fragments arranged by size:
1137 1010 776 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 810 868 810
Size: 58 4696
BsiEI CG_ry'CG
Cuts at: 104 2296 2342 3550 104
Size: 2192 46 1208 1308
Fragments arranged by size:
2192 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1768 2546 2736 3528 1768
Size: 778 190 792 2994
Fragments arranged by size:
2994 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1768 2379 2472 2546 2736 2798 3528 1768
Size: 611 93 74 190 62 730 2994
Fragments arranged by size:
2994 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3974 3974
Size: 4754
BspHI T'CATG_A
Cuts at: 3930 3930
Size: 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 980 1804 2018 2320 2701 980
Size: 824 214 302 381 3033
Fragments arranged by size:
3033 824 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3046 3100 4584 4749 50
Size: 2996 54 1484 165 55
Fragments arranged by size:
2996 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 287 2666 287
Size: 2379 2375
BsrFI r'CCGG_y
Cuts at: 2752 2933 3142 2752
Size: 181 209 4364
Fragments arranged by size:
4364 209 181
BssSI C'ACGA_G
Cuts at: 196 1171 3025 3383 196
Size: 975 1854 358 1567
Fragments arranged by size:
1854 1567 975 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2131 2131
Size: 4754
BstBI TT'CG_AA
Cuts at: 666 1496 3115 666
Size: 830 1619 2305
Fragments arranged by size:
2305 1619 830
BstEII G'GTnAC_C
Cuts at: 1249 1249
Size: 4754
BstYI r'GATC_y
Cuts at: 1 129 1160 1569 1779 2246 2269 2604 2850
Size: 128 1031 409 210 467 23 335 246
Cuts at: 2850 3862 3948 3960 4306 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1031 1012 467 449 409 346 335 246
210 128 86 23 12
BstZ17I GTA'TAC
Cuts at: 438 438
Size: 4754
BtgI C'CryG_G
Cuts at: 1180 1180
Size: 4754
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2704 2885 3048 2704
Size: 181 163 4410
Fragments arranged by size:
4410 181 163
BtsI GCAGTG_nn'
Cuts at: 911 1997 911
Size: 1086 3668
ClaI AT'CG_AT
Cuts at: 215 215
Size: 4754
DraI TTT'AAA
Cuts at: 945 1753 945
Size: 808 3946
DraIII CAC_nnn'GTG
Cuts at: 749 897 749
Size: 148 4606
DrdI GACnn_nn'nnGTC
Cuts at: 2460 3318 2460
Size: 858 3896
EaeI y'GGCC_r
Cuts at: 926 1354 2339 2513 2904 2931 4276 926
Size: 428 985 174 391 27 1345 1404
Fragments arranged by size:
1404 1345 985 428 391 174 27
EagI C'GGCC_G
Cuts at: 2339 2339
Size: 4754
EarI CTCTTCn'nnn_
Cuts at: 2777 2987 2777
Size: 210 4544
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2962 3272 3418 4564 2962
Size: 310 146 1146 3152
Fragments arranged by size:
3152 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1766 1766
Size: 4754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 2579 3011 3758 4026 856
Size: 1723 432 747 268 1584
Fragments arranged by size:
1723 1584 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1893 1893
Size: 4754
EcoRI G'AATT_C
Cuts at: 1758 1758
Size: 4754
EcoRV GAT'ATC
Cuts at: 285 1670 285
Size: 1385 3369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 4754
FspI TGC'GCA
Cuts at: 2535 2535
Size: 4754
HaeII r_GCGC'y
Cuts at: 2188 2436 3458 4437 2188
Size: 248 1022 979 2505
Fragments arranged by size:
2505 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 670 702 2596 2626 2628 2658 670
Size: 32 1894 30 2 30 2766
Fragments arranged by size:
2766 1894 32 30 30 2
HincII GTy'rAC
Cuts at: 815 1793 815
Size: 978 3776
KasI G'GCGC_C
Cuts at: 2184 2432 2184
Size: 248 4506
KpnI G_GTAC'C
Cuts at: 1774 1774
Size: 4754
MluI A'CGCG_T
Cuts at: 4187 4187
Size: 4754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 725 1305 3425 3609 725
Size: 580 2120 184 1870
Fragments arranged by size:
2120 1870 580 184
MscI TGG'CCA
Cuts at: 928 2515 928
Size: 1587 3167
MslI CAynn'nnrTG
Cuts at: 803 1538 2743 2870 803
Size: 735 1205 127 2687
Fragments arranged by size:
2687 1205 735 127
MspA1I CmG'CkG
Cuts at: 244 1865 2179 2539 3552 3797 244
Size: 1621 314 360 1013 245 1201
Fragments arranged by size:
1621 1201 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2935 2935
Size: 4754
NarI GG'CG_CC
Cuts at: 2185 2433 2185
Size: 248 4506
NcoI C'CATG_G
Cuts at: 1180 1180
Size: 4754
NdeI CA'TA_TG
Cuts at: 959 959
Size: 4754
NgoMIV G'CCGG_C
Cuts at: 2933 2933
Size: 4754
NspI r_CATG'y
Cuts at: 813 1537 1605 1807 2307 3214 813
Size: 724 68 202 500 907 2353
Fragments arranged by size:
2353 907 724 500 202 68
PciI A'CATG_T
Cuts at: 809 3210 809
Size: 2401 2353
PmeI GTTT'AAAC
Cuts at: 1753 1753
Size: 4754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 3893
Size: 32 4722
PspXI vC'TCGA_Gb
Cuts at: 1187 1187
Size: 4754
PstI C_TGCA'G
Cuts at: 1801 2486 1801
Size: 685 4069
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4754
PvuII CAG'CTG
Cuts at: 244 2179 2539 244
Size: 1935 360 2459
Fragments arranged by size:
2459 1935 360
RsrII CG'GwC_CG
Cuts at: 2949 2949
Size: 4754
SacI G_AGCT'C
Cuts at: 1768 1768
Size: 4754
SalI G'TCGA_C
Cuts at: 1791 1791
Size: 4754
SapI GCTCTTCn'nnn_
Cuts at: 2777 2987 2777
Size: 210 4544
SbfI CC_TGCA'GG
Cuts at: 1801 1801
Size: 4754
ScaI AGT'ACT
Cuts at: 4271 4271
Size: 4754
SexAI A'CCwGG_T
Cuts at: 1139 1361 1139
Size: 222 4532
SfcI C'TryA_G
Cuts at: 33 161 544 1710 1797 2482 3475 3666 33
Size: 128 383 1166 87 685 993 191 1121
Fragments arranged by size:
1166 1121 993 685 383 191 128 87
SfoI GGC'GCC
Cuts at: 2186 2434 2186
Size: 248 4506
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4754
SmaI CCC'GGG
Cuts at: 1776 1776
Size: 4754
SmlI C'TyrA_G
Cuts at: 1187 1909 2824 3316 3578 1187
Size: 722 915 492 262 2363
Fragments arranged by size:
2363 915 722 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4754
SphI G_CATG'C
Cuts at: 1605 1807 2307 1605
Size: 202 500 4052
Fragments arranged by size:
4052 500 202
SspI AAT'ATT
Cuts at: 708 708
Size: 4754
StyI C'CwwG_G
Cuts at: 1180 1888 2242 1180
Size: 708 354 3692
Fragments arranged by size:
3692 708 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 493 607 1492 2337 2470 2929 3138 493
Size: 114 885 845 133 459 209 2109
Fragments arranged by size:
2109 885 845 459 209 133 114
TatI w'GTAC_w
Cuts at: 1200 4269 1200
Size: 3069 1685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4271 4303 4271
Size: 32 4722
Tth111I GACn'n_nGTC
Cuts at: 2551 2551
Size: 4754
XbaI T'CTAG_A
Cuts at: 1785 1785
Size: 4754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 487 2249 487
Size: 1762 2992
XhoI C'TCGA_G
Cuts at: 1187 1187
Size: 4754
XmaI C'CCGG_G
Cuts at: 1774 1774
Size: 4754
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstXI Bsu36I CspCI EcoNI FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XmnI
ZraI