Restriction map for
pF1KB8193
(Circular) (Six-base) MAPSORT of: pF1KB8193.seq Check: 4140 from: 1 to: 3470
pF1KB8193 3470 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 508 508
Size: 3470
Acc65I G'GTAC_C
Cuts at: 270 486 270
Size: 216 3254
AclI AA'CG_TT
Cuts at: 3310 3310
Size: 3470
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1295 1727 2474 2742 1295
Size: 432 747 268 2023
Fragments arranged by size:
2023 747 432 268
AflIII A'CryG_T
Cuts at: 822 1926 2903 822
Size: 1104 977 1389
Fragments arranged by size:
1389 1104 977
AgeI A'CCGG_T
Cuts at: 1858 1858
Size: 3470
AlwNI CAG_nnn'CTG
Cuts at: 373 775 2342 373
Size: 402 1567 1501
Fragments arranged by size:
1567 1501 402
ApaI G_GGCC'C
Cuts at: 202 202
Size: 3470
ApaLI G'TGCA_C
Cuts at: 2240 2240
Size: 3470
ApoI r'AATT_y
Cuts at: 474 2823 3079 474
Size: 2349 256 865
Fragments arranged by size:
2349 865 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3470
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3470
AvaI C'yCGr_G
Cuts at: 182 282 490 182
Size: 100 208 3162
Fragments arranged by size:
3162 208 100
BamHI G'GATC_C
Cuts at: 495 495
Size: 3470
BanI G'GyrC_C
Cuts at: 270 486 900 1148 1183 270
Size: 216 414 248 35 2557
Fragments arranged by size:
2557 414 248 216 35
BanII G_rGCy'C
Cuts at: 124 202 419 484 1514 124
Size: 78 217 65 1030 2080
Fragments arranged by size:
2080 1030 217 78 65
BbeI G_GCGC'C
Cuts at: 904 1152 904
Size: 248 3222
BbvCI CC'TCA_GC
Cuts at: 209 209
Size: 3470
BciVI GTATCCnnnnn_n'
Cuts at: 1368 2129 3470 1368
Size: 761 1341 1368
Fragments arranged by size:
1368 1341 761
BclI T'GATC_A
Cuts at: 990 990
Size: 3470
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3470
BglII A'GATC_T
Cuts at: 985 3022 985
Size: 2037 1433
BlpI GC'TnA_GC
Cuts at: 118 220 298 582 118
Size: 102 78 284 3006
Fragments arranged by size:
3006 284 102 78
Bme1580I G_kGCm'C
Cuts at: 202 1095 1188 2244 202
Size: 893 93 1056 1428
Fragments arranged by size:
1428 1056 893 93
BmrI ACTGGGnnnn_n'
Cuts at: 682 763 1098 3242 682
Size: 81 335 2144 910
Fragments arranged by size:
2144 910 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 308 1837 308
Size: 1529 1941
Bpu10I CC'TnA_GC
Cuts at: 209 209
Size: 3470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1525 2017 2315 646
Size: 879 492 298 1801
Fragments arranged by size:
1801 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3174 3174
Size: 3470
BsaAI yAC'GTr
Cuts at: 823 823
Size: 3470
BsaBI GATnn'nnATC
Cuts at: 6 984 3021 6
Size: 978 2037 455
Fragments arranged by size:
2037 978 455
BsaHI Gr'CG_yC
Cuts at: 901 1149 1851 3347 901
Size: 248 702 1496 1024
Fragments arranged by size:
1496 1024 702 248
BsaWI w'CCGG_w
Cuts at: 423 659 1180 1858 2132 2279 2690 423
Size: 236 521 678 274 147 411 1203
Fragments arranged by size:
1203 678 521 411 274 236 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 235 265 235
Size: 30 3440
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 226
Size: 3470
BseYI C'CCAG_C
Cuts at: 895 1653 2230 2726 895
Size: 758 577 496 1639
Fragments arranged by size:
1639 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1012 1058 2266 104
Size: 908 46 1208 1308
Fragments arranged by size:
1308 1208 908 46
BsiHKAI G_wGCw'C
Cuts at: 419 484 1262 1452 2244 419
Size: 65 778 190 792 1645
Fragments arranged by size:
1645 792 778 190 65
Bsp1286I G_dGCh'C
Cuts at: 124 202 419 484 1095 1188 1262 1452 1514
Size: 78 217 65 611 93 74 190 62
Cuts at: 1514 2244 124
Size: 730 1350
Fragments arranged by size:
1350 730 611 217 190 93 78 74
65 62
BspEI T'CCGG_A
Cuts at: 423 2690 423
Size: 2267 1203
BspHI T'CATG_A
Cuts at: 2646 2646
Size: 3470
BspMI ACCTGCnnnn'nnnn_
Cuts at: 339 353 520 734 1036 1417 339
Size: 14 167 214 302 381 2392
Fragments arranged by size:
2392 381 302 214 167 14
BsrBI CCG'CTC
Cuts at: 50 1762 1816 3300 3465 50
Size: 1712 54 1484 165 55
Fragments arranged by size:
1712 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1382 1382
Size: 3470
BsrFI r'CCGG_y
Cuts at: 1468 1649 1858 1468
Size: 181 209 3080
Fragments arranged by size:
3080 209 181
BssSI C'ACGA_G
Cuts at: 1741 2099 1741
Size: 358 3112
BstAPI GCAn_nnn'nTGC
Cuts at: 847 847
Size: 3470
BstBI TT'CG_AA
Cuts at: 1831 1831
Size: 3470
BstYI r'GATC_y
Cuts at: 1 495 962 985 1320 1566 2578 2664 2676
Size: 494 467 23 335 246 1012 86 12
Cuts at: 2676 3022 1
Size: 346 449
Fragments arranged by size:
1012 494 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 162 1420 1601 1764 162
Size: 1258 181 163 1868
Fragments arranged by size:
1868 1258 181 163
BtsI GCAGTG_nn'
Cuts at: 243 713 243
Size: 470 3000
DraI TTT'AAA
Cuts at: 469 469
Size: 3470
DrdI GACnn_nn'nnGTC
Cuts at: 1176 2034 1176
Size: 858 2612
EaeI y'GGCC_r
Cuts at: 173 359 1055 1229 1620 1647 2992 173
Size: 186 696 174 391 27 1345 651
Fragments arranged by size:
1345 696 651 391 186 174 27
EagI C'GGCC_G
Cuts at: 1055 1055
Size: 3470
EarI CTCTTCn'nnn_
Cuts at: 1493 1703 1493
Size: 210 3260
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1678 1988 2134 3280 1678
Size: 310 146 1146 1868
Fragments arranged by size:
1868 1146 310 146
EcoICRI GAG'CTC
Cuts at: 417 482 417
Size: 65 3405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1295 1727 2474 2742 1295
Size: 432 747 268 2023
Fragments arranged by size:
2023 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 198 609 198
Size: 411 3059
EcoRI G'AATT_C
Cuts at: 474 474
Size: 3470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1489 1489
Size: 3470
FspI TGC'GCA
Cuts at: 1251 1251
Size: 3470
HaeII r_GCGC'y
Cuts at: 118 904 1152 2174 3153 118
Size: 786 248 1022 979 435
Fragments arranged by size:
1022 979 786 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1312 1342 1344 1374 1312
Size: 30 2 30 3408
Fragments arranged by size:
3408 30 30 2
HincII GTy'rAC
Cuts at: 509 509
Size: 3470
KasI G'GCGC_C
Cuts at: 900 1148 900
Size: 248 3222
KpnI G_GTAC'C
Cuts at: 274 490 274
Size: 216 3254
MluI A'CGCG_T
Cuts at: 2903 2903
Size: 3470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 464 2141 2325 140
Size: 324 1677 184 1285
Fragments arranged by size:
1677 1285 324 184
MscI TGG'CCA
Cuts at: 175 1231 175
Size: 1056 2414
MslI CAynn'nnrTG
Cuts at: 1459 1586 1459
Size: 127 3343
MspA1I CmG'CkG
Cuts at: 208 219 581 895 1255 2268 2513 208
Size: 11 362 314 360 1013 245 1165
Fragments arranged by size:
1165 1013 362 360 314 245 11
NaeI GCC'GGC
Cuts at: 1651 1651
Size: 3470
NarI GG'CG_CC
Cuts at: 901 1149 901
Size: 248 3222
NgoMIV G'CCGG_C
Cuts at: 1649 1649
Size: 3470
NspI r_CATG'y
Cuts at: 523 1023 1930 523
Size: 500 907 2063
Fragments arranged by size:
2063 907 500
PciI A'CATG_T
Cuts at: 1926 1926
Size: 3470
PflMI CCAn_nnn'nTGG
Cuts at: 373 373
Size: 3470
PmeI GTTT'AAAC
Cuts at: 469 469
Size: 3470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 3438
PspOMI G'GGCC_C
Cuts at: 198 198
Size: 3470
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 232 264 232
Size: 32 3438
PstI C_TGCA'G
Cuts at: 350 517 1202 350
Size: 167 685 2618
Fragments arranged by size:
2618 685 167
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3470
PvuII CAG'CTG
Cuts at: 208 895 1255 208
Size: 687 360 2423
Fragments arranged by size:
2423 687 360
RsrII CG'GwC_CG
Cuts at: 1665 1665
Size: 3470
SacI G_AGCT'C
Cuts at: 419 484 419
Size: 65 3405
SalI G'TCGA_C
Cuts at: 507 507
Size: 3470
SapI GCTCTTCn'nnn_
Cuts at: 1493 1703 1493
Size: 210 3260
SbfI CC_TGCA'GG
Cuts at: 350 517 350
Size: 167 3303
ScaI AGT'ACT
Cuts at: 2987 2987
Size: 3470
SfcI C'TryA_G
Cuts at: 33 316 346 513 1198 2191 2382 33
Size: 283 30 167 685 993 191 1121
Fragments arranged by size:
1121 993 685 283 191 167 30
SfoI GGC'GCC
Cuts at: 902 1150 902
Size: 248 3222
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3470
SmaI CCC'GGG
Cuts at: 184 492 184
Size: 308 3162
SmlI C'TyrA_G
Cuts at: 625 1540 2032 2294 625
Size: 915 492 262 1801
Fragments arranged by size:
1801 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3470
SphI G_CATG'C
Cuts at: 523 1023 523
Size: 500 2970
StyI C'CwwG_G
Cuts at: 604 958 604
Size: 354 3116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1053 1186 1645 1854 1053
Size: 133 459 209 2669
Fragments arranged by size:
2669 459 209 133
TatI w'GTAC_w
Cuts at: 2985 2985
Size: 3470
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 235 267 2987 3019 235
Size: 32 2720 32 686
Fragments arranged by size:
2720 686 32 32
Tth111I GACn'n_nGTC
Cuts at: 1267 1267
Size: 3470
XbaI T'CTAG_A
Cuts at: 501 501
Size: 3470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 965 965
Size: 3470
XmaI C'CCGG_G
Cuts at: 182 490 182
Size: 308 3162
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI
PpiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BpmI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI