Restriction map for
pF1KB8192
(Circular) (Six-base) MAPSORT of: pF1KB8192.seq Check: 187 from: 1 to: 3785
pF1KB8192 3785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 823 823
Size: 3785
Acc65I G'GTAC_C
Cuts at: 801 801
Size: 3785
AclI AA'CG_TT
Cuts at: 3625 3625
Size: 3785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 1610 2042 2789 3057 425
Size: 1185 432 747 268 1153
Fragments arranged by size:
1185 1153 747 432 268
AflIII A'CryG_T
Cuts at: 647 1137 2241 3218 647
Size: 490 1104 977 1214
Fragments arranged by size:
1214 1104 977 490
AgeI A'CCGG_T
Cuts at: 2173 2173
Size: 3785
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 532 564 532
Size: 32 3753
AlwNI CAG_nnn'CTG
Cuts at: 165 1090 2657 165
Size: 925 1567 1293
Fragments arranged by size:
1567 1293 925
ApaLI G'TGCA_C
Cuts at: 2555 2555
Size: 3785
ApoI r'AATT_y
Cuts at: 193 233 789 3138 3394 193
Size: 40 556 2349 256 584
Fragments arranged by size:
2349 584 556 256 40
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 805 805
Size: 3785
BamHI G'GATC_C
Cuts at: 810 810
Size: 3785
BanI G'GyrC_C
Cuts at: 801 1215 1463 1498 801
Size: 414 248 35 3088
Fragments arranged by size:
3088 414 248 35
BanII G_rGCy'C
Cuts at: 799 1829 799
Size: 1030 2755
BbeI G_GCGC'C
Cuts at: 1219 1467 1219
Size: 248 3537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3751
BciVI GTATCCnnnnn_n'
Cuts at: 638 1683 2444 3785 638
Size: 1045 761 1341 638
Fragments arranged by size:
1341 1045 761 638
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3785
BglII A'GATC_T
Cuts at: 1300 3337 1300
Size: 2037 1748
BlpI GC'TnA_GC
Cuts at: 897 897
Size: 3785
Bme1580I G_kGCm'C
Cuts at: 1410 1503 2559 1410
Size: 93 1056 2636
Fragments arranged by size:
2636 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 538 997 1078 1413 3557 538
Size: 459 81 335 2144 766
Fragments arranged by size:
2144 766 459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 3785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 187 167
Size: 20 3765
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 741 961 1840 2332 2630 349
Size: 392 220 879 492 298 1504
Fragments arranged by size:
1504 879 492 392 298 220
BsaI GGTCTCn'nnnn_
Cuts at: 483 3489 483
Size: 3006 779
BsaAI yAC'GTr
Cuts at: 1138 1138
Size: 3785
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 1216 1464 2166 3662 1216
Size: 248 702 1496 1339
Fragments arranged by size:
1496 1339 702 248
BsaWI w'CCGG_w
Cuts at: 974 1495 2173 2447 2594 3005 974
Size: 521 678 274 147 411 1754
Fragments arranged by size:
1754 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 390 744 390
Size: 354 3431
BseYI C'CCAG_C
Cuts at: 1210 1968 2545 3041 1210
Size: 758 577 496 1954
Fragments arranged by size:
1954 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1327 1373 2581 104
Size: 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 46
BsiHKAI G_wGCw'C
Cuts at: 459 799 1577 1767 2559 459
Size: 340 778 190 792 1685
Fragments arranged by size:
1685 792 778 340 190
Bsp1286I G_dGCh'C
Cuts at: 459 799 1410 1503 1577 1767 1829 2559 459
Size: 340 611 93 74 190 62 730 1685
Fragments arranged by size:
1685 730 611 340 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3005 3005
Size: 3785
BspHI T'CATG_A
Cuts at: 240 2961 240
Size: 2721 1064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 835 1049 1351 1732 835
Size: 214 302 381 2888
Fragments arranged by size:
2888 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2077 2131 3615 3780 50
Size: 2027 54 1484 165 55
Fragments arranged by size:
2027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 1697
Size: 3785
BsrFI r'CCGG_y
Cuts at: 1783 1964 2173 1783
Size: 181 209 3395
Fragments arranged by size:
3395 209 181
BssSI C'ACGA_G
Cuts at: 2056 2414 2056
Size: 358 3427
BstAPI GCAn_nnn'nTGC
Cuts at: 445 1162 445
Size: 717 3068
BstBI TT'CG_AA
Cuts at: 2146 2146
Size: 3785
BstXI CCAn_nnnn'nTGG
Cuts at: 467 467
Size: 3785
BstYI r'GATC_y
Cuts at: 1 810 1277 1300 1635 1881 2893 2979 2991
Size: 809 467 23 335 246 1012 86 12
Cuts at: 2991 3337 1
Size: 346 449
Fragments arranged by size:
1012 809 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 631 631
Size: 3785
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1735 1916 2079 1735
Size: 181 163 3441
Fragments arranged by size:
3441 181 163
BtsI GCAGTG_nn'
Cuts at: 1028 1028
Size: 3785
DraI TTT'AAA
Cuts at: 784 784
Size: 3785
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2349 1491
Size: 858 2927
EaeI y'GGCC_r
Cuts at: 567 1370 1544 1935 1962 3307 567
Size: 803 174 391 27 1345 1045
Fragments arranged by size:
1345 1045 803 391 174 27
EagI C'GGCC_G
Cuts at: 1370 1370
Size: 3785
EarI CTCTTCn'nnn_
Cuts at: 268 1808 2018 268
Size: 1540 210 2035
Fragments arranged by size:
2035 1540 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1993 2303 2449 3595 1993
Size: 310 146 1146 2183
Fragments arranged by size:
2183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 797 797
Size: 3785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 187 425 1610 2042 2789 3057 167
Size: 20 238 1185 432 747 268 895
Fragments arranged by size:
1185 895 747 432 268 238 20
EcoO109I rG'GnC_Cy
Cuts at: 162 480 924 162
Size: 318 444 3023
Fragments arranged by size:
3023 444 318
EcoRI G'AATT_C
Cuts at: 789 789
Size: 3785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3785
FspI TGC'GCA
Cuts at: 1566 1566
Size: 3785
HaeII r_GCGC'y
Cuts at: 1219 1467 2489 3468 1219
Size: 248 1022 979 1536
Fragments arranged by size:
1536 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 528 560 727 759 1627 1657 1659 1689 528
Size: 32 167 32 868 30 2 30 2624
Fragments arranged by size:
2624 868 167 32 32 30 30 2
HincII GTy'rAC
Cuts at: 746 824 746
Size: 78 3707
KasI G'GCGC_C
Cuts at: 1215 1463 1215
Size: 248 3537
KpnI G_GTAC'C
Cuts at: 805 805
Size: 3785
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 674 2456 2640 674
Size: 1782 184 1819
Fragments arranged by size:
1819 1782 184
MscI TGG'CCA
Cuts at: 569 1546 569
Size: 977 2808
MslI CAynn'nnrTG
Cuts at: 185 652 680 1774 1901 185
Size: 467 28 1094 127 2069
Fragments arranged by size:
2069 1094 467 127 28
MspA1I CmG'CkG
Cuts at: 340 896 1210 1570 2583 2828 340
Size: 556 314 360 1013 245 1297
Fragments arranged by size:
1297 1013 556 360 314 245
NaeI GCC'GGC
Cuts at: 1966 1966
Size: 3785
NarI GG'CG_CC
Cuts at: 1216 1464 1216
Size: 248 3537
NcoI C'CATG_G
Cuts at: 631 631
Size: 3785
NgoMIV G'CCGG_C
Cuts at: 1964 1964
Size: 3785
NspI r_CATG'y
Cuts at: 651 838 1338 2245 651
Size: 187 500 907 2191
Fragments arranged by size:
2191 907 500 187
PciI A'CATG_T
Cuts at: 647 2241 647
Size: 1594 2191
PflMI CCAn_nnn'nTGG
Cuts at: 165 165
Size: 3785
PfoI T'CCnGG_A
Cuts at: 164 164
Size: 3785
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PpuMI rG'GwC_Cy
Cuts at: 162 480 162
Size: 318 3467
PstI C_TGCA'G
Cuts at: 832 1517 832
Size: 685 3100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 340 1210 1570 340
Size: 870 360 2555
Fragments arranged by size:
2555 870 360
RsrII CG'GwC_CG
Cuts at: 1980 1980
Size: 3785
SacI G_AGCT'C
Cuts at: 799 799
Size: 3785
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SanDI GG'GwC_CC
Cuts at: 480 480
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 1808 2018 1808
Size: 210 3575
SbfI CC_TGCA'GG
Cuts at: 832 832
Size: 3785
ScaI AGT'ACT
Cuts at: 3302 3302
Size: 3785
SexAI A'CCwGG_T
Cuts at: 158 158
Size: 3785
SfcI C'TryA_G
Cuts at: 33 828 1513 2506 2697 33
Size: 795 685 993 191 1121
Fragments arranged by size:
1121 993 795 685 191
SfoI GGC'GCC
Cuts at: 1217 1465 1217
Size: 248 3537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SmaI CCC'GGG
Cuts at: 807 807
Size: 3785
SmlI C'TyrA_G
Cuts at: 364 756 940 1855 2347 2609 364
Size: 392 184 915 492 262 1540
Fragments arranged by size:
1540 915 492 392 262 184
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 838 1338 838
Size: 500 3285
StyI C'CwwG_G
Cuts at: 631 919 1273 631
Size: 288 354 3143
Fragments arranged by size:
3143 354 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 1501 1960 2169 1368
Size: 133 459 209 2984
Fragments arranged by size:
2984 459 209 133
TatI w'GTAC_w
Cuts at: 3300 3300
Size: 3785
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 3753
Tth111I GACn'n_nGTC
Cuts at: 1582 1582
Size: 3785
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 629 1280 629
Size: 651 3134
XmaI C'CCGG_G
Cuts at: 805 805
Size: 3785
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI