Restriction map for
pF1KB8187
(Circular) (Six-base) MAPSORT of: pF1KB8187.seq Check: 694 from: 1 to: 4469
pF1KB8187 4469 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 897 1173 897
Size: 276 4193
AccI GT'mk_AC
Cuts at: 1507 1507
Size: 4469
Acc65I G'GTAC_C
Cuts at: 1275 1485 1275
Size: 210 4259
AclI AA'CG_TT
Cuts at: 4309 4309
Size: 4469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1223 2294 2726 3473 3741 793
Size: 430 1071 432 747 268 1521
Fragments arranged by size:
1521 1071 747 432 430 268
AflIII A'CryG_T
Cuts at: 1229 1821 2925 3902 1229
Size: 592 1104 977 1796
Fragments arranged by size:
1796 1104 977 592
AgeI A'CCGG_T
Cuts at: 2857 2857
Size: 4469
AlwNI CAG_nnn'CTG
Cuts at: 491 1774 3341 491
Size: 1283 1567 1619
Fragments arranged by size:
1619 1567 1283
ApaI G_GGCC'C
Cuts at: 480 480
Size: 4469
ApaLI G'TGCA_C
Cuts at: 3239 3239
Size: 4469
ApoI r'AATT_y
Cuts at: 1420 1473 3822 4078 1420
Size: 53 2349 256 1811
Fragments arranged by size:
2349 1811 256 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 4469
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
AvaI C'yCGr_G
Cuts at: 1489 1489
Size: 4469
BamHI G'GATC_C
Cuts at: 931 1494 931
Size: 563 3906
BanI G'GyrC_C
Cuts at: 531 871 958 1275 1485 1899 2147 2182 531
Size: 340 87 317 210 414 248 35 2818
Fragments arranged by size:
2818 414 340 317 248 210 87 35
BanII G_rGCy'C
Cuts at: 480 1046 1483 2513 480
Size: 566 437 1030 2436
Fragments arranged by size:
2436 1030 566 437
BbeI G_GCGC'C
Cuts at: 1903 2151 1903
Size: 248 4221
BbsI GAAGACnn'nnnn_
Cuts at: 1211 1406 1211
Size: 195 4274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 129 163 948 982 129
Size: 34 785 34 3616
Fragments arranged by size:
3616 785 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2367 3128 4469 2367
Size: 761 1341 2367
Fragments arranged by size:
2367 1341 761
BclI T'GATC_A
Cuts at: 266 1989 266
Size: 1723 2746
BglI GCCn_nnn'nGGC
Cuts at: 137 239 137
Size: 102 4367
BglII A'GATC_T
Cuts at: 1385 1984 4021 1385
Size: 599 2037 1833
Fragments arranged by size:
2037 1833 599
BlpI GC'TnA_GC
Cuts at: 1221 1581 1221
Size: 360 4109
Bme1580I G_kGCm'C
Cuts at: 282 416 480 2094 2187 3243 282
Size: 134 64 1614 93 1056 1508
Fragments arranged by size:
1614 1508 1056 134 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 234 856 1681 1762 2097 4241 234
Size: 622 825 81 335 2144 462
Fragments arranged by size:
2144 825 622 462 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 261 565 732 2836 261
Size: 304 167 2104 1894
Fragments arranged by size:
2104 1894 304 167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 740 807 740
Size: 67 4402
Bpu10I CC'TnA_GC
Cuts at: 832 832
Size: 4469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 1645 2524 3016 3314 1465
Size: 180 879 492 298 2620
Fragments arranged by size:
2620 879 492 298 180
BsaI GGTCTCn'nnnn_
Cuts at: 4173 4173
Size: 4469
BsaAI yAC'GTr
Cuts at: 1822 1822
Size: 4469
BsaBI GATnn'nnATC
Cuts at: 6 265 1983 4020 6
Size: 259 1718 2037 455
Fragments arranged by size:
2037 1718 455 259
BsaHI Gr'CG_yC
Cuts at: 1900 2148 2850 4346 1900
Size: 248 702 1496 2023
Fragments arranged by size:
2023 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1658 2179 2857 3131 3278 3689 1658
Size: 521 678 274 147 411 2438
Fragments arranged by size:
2438 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 884 884
Size: 4469
BseYI C'CCAG_C
Cuts at: 1894 2652 3229 3725 1894
Size: 758 577 496 2638
Fragments arranged by size:
2638 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 4469
BsiEI CG_ry'CG
Cuts at: 104 2011 2057 3265 104
Size: 1907 46 1208 1308
Fragments arranged by size:
1907 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 860 1483 2261 2451 3243 860
Size: 623 778 190 792 2086
Fragments arranged by size:
2086 792 778 623 190
Bsp1286I G_dGCh'C
Cuts at: 282 416 480 860 1046 1483 2094 2187 2261
Size: 134 64 380 186 437 611 93 74
Cuts at: 2261 2451 2513 3243 282
Size: 190 62 730 1508
Fragments arranged by size:
1508 730 611 437 380 190 186 134
93 74 64 62
BspEI T'CCGG_A
Cuts at: 3689 3689
Size: 4469
BspHI T'CATG_A
Cuts at: 1379 3645 1379
Size: 2266 2203
BspMI ACCTGCnnnn'nnnn_
Cuts at: 897 1173 1519 1733 2035 2416 897
Size: 276 346 214 302 381 2950
Fragments arranged by size:
2950 381 346 302 276 214
BsrBI CCG'CTC
Cuts at: 50 245 558 2761 2815 4299 4464 50
Size: 195 313 2203 54 1484 165 55
Fragments arranged by size:
2203 1484 313 195 165 55 54
BsrDI GCAATG_nn'
Cuts at: 756 2381 756
Size: 1625 2844
BsrFI r'CCGG_y
Cuts at: 2467 2648 2857 2467
Size: 181 209 4079
Fragments arranged by size:
4079 209 181
BsrGI T'GTAC_A
Cuts at: 354 354
Size: 4469
BssSI C'ACGA_G
Cuts at: 2740 3098 2740
Size: 358 4111
BstAPI GCAn_nnn'nTGC
Cuts at: 1846 1846
Size: 4469
BstBI TT'CG_AA
Cuts at: 2830 2830
Size: 4469
BstXI CCAn_nnnn'nTGG
Cuts at: 1147 1147
Size: 4469
BstYI r'GATC_y
Cuts at: 1 931 1385 1494 1961 1984 2319 2565 3577
Size: 930 454 109 467 23 335 246 1012
Cuts at: 3577 3663 3675 4021 1
Size: 86 12 346 449
Fragments arranged by size:
1012 930 467 454 449 346 335 246
109 86 23 12
BtgI C'CryG_G
Cuts at: 107 300 107
Size: 193 4276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2419 2600 2763 2419
Size: 181 163 4125
Fragments arranged by size:
4125 181 163
BtsI GCAGTG_nn'
Cuts at: 398 1250 1712 398
Size: 852 462 3155
Fragments arranged by size:
3155 852 462
DraI TTT'AAA
Cuts at: 1468 1468
Size: 4469
DraIII CAC_nnn'GTG
Cuts at: 128 128
Size: 4469
DrdI GACnn_nn'nnGTC
Cuts at: 2175 3033 2175
Size: 858 3611
EaeI y'GGCC_r
Cuts at: 2054 2228 2619 2646 3991 2054
Size: 174 391 27 1345 2532
Fragments arranged by size:
2532 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2054 2054
Size: 4469
EarI CTCTTCn'nnn_
Cuts at: 793 812 2492 2702 793
Size: 19 1680 210 2560
Fragments arranged by size:
2560 1680 210 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2677 2987 3133 4279 2677
Size: 310 146 1146 2867
Fragments arranged by size:
2867 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1481 1481
Size: 4469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 740 793 807 1223 2294 2726 3473 3741 740
Size: 53 14 416 1071 432 747 268 1468
Fragments arranged by size:
1468 1071 747 432 416 268 53 14
EcoNI CCTnn'n_nnAGG
Cuts at: 190 190
Size: 4469
EcoO109I rG'GnC_Cy
Cuts at: 459 476 477 1608 459
Size: 17 1 1131 3320
Fragments arranged by size:
3320 1131 17 1
EcoRI G'AATT_C
Cuts at: 1473 1473
Size: 4469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4469
FspI TGC'GCA
Cuts at: 2250 2250
Size: 4469
HaeII r_GCGC'y
Cuts at: 1903 2151 3173 4152 1903
Size: 248 1022 979 2220
Fragments arranged by size:
2220 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1049 1081 2311 2341 2343 2373 1049
Size: 32 1230 30 2 30 3145
Fragments arranged by size:
3145 1230 32 30 30 2
HincII GTy'rAC
Cuts at: 1508 1508
Size: 4469
KasI G'GCGC_C
Cuts at: 1899 2147 1899
Size: 248 4221
KpnI G_GTAC'C
Cuts at: 1279 1489 1279
Size: 210 4259
MluI A'CGCG_T
Cuts at: 3902 3902
Size: 4469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3140 3324 3140
Size: 184 4285
MscI TGG'CCA
Cuts at: 2230 2230
Size: 4469
MslI CAynn'nnrTG
Cuts at: 407 760 851 1432 2458 2585 407
Size: 353 91 581 1026 127 2291
Fragments arranged by size:
2291 1026 581 353 127 91
MspA1I CmG'CkG
Cuts at: 740 1580 1894 2254 3267 3512 740
Size: 840 314 360 1013 245 1697
Fragments arranged by size:
1697 1013 840 360 314 245
NaeI GCC'GGC
Cuts at: 2650 2650
Size: 4469
NarI GG'CG_CC
Cuts at: 1900 2148 1900
Size: 248 4221
NcoI C'CATG_G
Cuts at: 107 107
Size: 4469
NdeI CA'TA_TG
Cuts at: 927 927
Size: 4469
NgoMIV G'CCGG_C
Cuts at: 2648 2648
Size: 4469
NspI r_CATG'y
Cuts at: 1063 1158 1233 1522 2022 2929 1063
Size: 95 75 289 500 907 2603
Fragments arranged by size:
2603 907 500 289 95 75
PciI A'CATG_T
Cuts at: 1229 2925 1229
Size: 1696 2773
PmeI GTTT'AAAC
Cuts at: 1468 1468
Size: 4469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 646 678 3608 3640 646
Size: 32 2930 32 1475
Fragments arranged by size:
2930 1475 32 32
PpuMI rG'GwC_Cy
Cuts at: 459 459
Size: 4469
PspOMI G'GGCC_C
Cuts at: 476 476
Size: 4469
PstI C_TGCA'G
Cuts at: 1193 1516 2201 1193
Size: 323 685 3461
Fragments arranged by size:
3461 685 323
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4469
PvuII CAG'CTG
Cuts at: 1894 2254 1894
Size: 360 4109
RsrII CG'GwC_CG
Cuts at: 2664 2664
Size: 4469
SacI G_AGCT'C
Cuts at: 1483 1483
Size: 4469
SalI G'TCGA_C
Cuts at: 1506 1506
Size: 4469
SapI GCTCTTCn'nnn_
Cuts at: 793 2492 2702 793
Size: 1699 210 2560
Fragments arranged by size:
2560 1699 210
SbfI CC_TGCA'GG
Cuts at: 1516 1516
Size: 4469
ScaI AGT'ACT
Cuts at: 3986 3986
Size: 4469
SexAI A'CCwGG_T
Cuts at: 461 1350 461
Size: 889 3580
SfcI C'TryA_G
Cuts at: 33 1189 1512 2197 3190 3381 33
Size: 1156 323 685 993 191 1121
Fragments arranged by size:
1156 1121 993 685 323 191
SfoI GGC'GCC
Cuts at: 1901 2149 1901
Size: 248 4221
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
SmaI CCC'GGG
Cuts at: 1491 1491
Size: 4469
SmlI C'TyrA_G
Cuts at: 1444 1624 2539 3031 3293 1444
Size: 180 915 492 262 2620
Fragments arranged by size:
2620 915 492 262 180
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4469
SphI G_CATG'C
Cuts at: 1522 2022 1522
Size: 500 3969
StuI AGG'CCT
Cuts at: 1331 1331
Size: 4469
StyI C'CwwG_G
Cuts at: 107 527 1603 1957 107
Size: 420 1076 354 2619
Fragments arranged by size:
2619 1076 420 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2052 2185 2644 2853 2052
Size: 133 459 209 3668
Fragments arranged by size:
3668 459 209 133
TatI w'GTAC_w
Cuts at: 354 371 790 945 3984 354
Size: 17 419 155 3039 839
Fragments arranged by size:
3039 839 419 155 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1301 1333 3986 4018 1301
Size: 32 2653 32 1752
Fragments arranged by size:
2653 1752 32 32
Tth111I GACn'n_nGTC
Cuts at: 757 2266 757
Size: 1509 2960
XbaI T'CTAG_A
Cuts at: 1500 1500
Size: 4469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 969 1964 969
Size: 995 3474
XmaI C'CCGG_G
Cuts at: 1489 1489
Size: 4469
XmnI GAAnn'nnTTC
Cuts at: 1331 1331
Size: 4469
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI