Restriction map for
pF1KB8186
(Circular) (Six-base) MAPSORT of: pF1KB8186.seq Check: 423 from: 1 to: 4022
pF1KB8186 4022 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1060 1060
Size: 4022
Acc65I G'GTAC_C
Cuts at: 1038 1038
Size: 4022
AclI AA'CG_TT
Cuts at: 3862 3862
Size: 4022
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1847 2279 3026 3294 1847
Size: 432 747 268 2575
Fragments arranged by size:
2575 747 432 268
AflIII A'CryG_T
Cuts at: 1374 2478 3455 1374
Size: 1104 977 1941
Fragments arranged by size:
1941 1104 977
AgeI A'CCGG_T
Cuts at: 190 2410 190
Size: 2220 1802
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 871 871
Size: 4022
AlwNI CAG_nnn'CTG
Cuts at: 879 1327 2894 879
Size: 448 1567 2007
Fragments arranged by size:
2007 1567 448
ApaLI G'TGCA_C
Cuts at: 744 2792 744
Size: 2048 1974
ApoI r'AATT_y
Cuts at: 148 295 368 920 1026 3375 3631 148
Size: 147 73 552 106 2349 256 539
Fragments arranged by size:
2349 552 539 256 147 106 73
AseI AT'TA_AT
Cuts at: 20 20
Size: 4022
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
AvaI C'yCGr_G
Cuts at: 1042 1042
Size: 4022
BamHI G'GATC_C
Cuts at: 1047 1047
Size: 4022
BanI G'GyrC_C
Cuts at: 1038 1452 1700 1735 1038
Size: 414 248 35 3325
Fragments arranged by size:
3325 414 248 35
BanII G_rGCy'C
Cuts at: 1036 2066 1036
Size: 1030 2992
BbeI G_GCGC'C
Cuts at: 1456 1704 1456
Size: 248 3774
BbsI GAAGACnn'nnnn_
Cuts at: 367 367
Size: 4022
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 240 274 533 567 240
Size: 34 259 34 3695
Fragments arranged by size:
3695 259 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1920 2681 4022 1920
Size: 761 1341 1920
Fragments arranged by size:
1920 1341 761
BclI T'GATC_A
Cuts at: 1542 1542
Size: 4022
BglI GCCn_nnn'nGGC
Cuts at: 248 248
Size: 4022
BglII A'GATC_T
Cuts at: 1537 3574 1537
Size: 2037 1985
BlpI GC'TnA_GC
Cuts at: 1134 1134
Size: 4022
Bme1580I G_kGCm'C
Cuts at: 497 748 1647 1740 2796 497
Size: 251 899 93 1056 1723
Fragments arranged by size:
1723 1056 899 251 93
BmrI ACTGGGnnnn_n'
Cuts at: 491 700 811 1234 1315 1650 3794 491
Size: 209 111 423 81 335 2144 719
Fragments arranged by size:
2144 719 423 335 209 111 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4022
Bpu10I CC'TnA_GC
Cuts at: 671 671
Size: 4022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1198 2077 2569 2867 1198
Size: 879 492 298 2353
Fragments arranged by size:
2353 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3726 3726
Size: 4022
BsaAI yAC'GTr
Cuts at: 225 1375 225
Size: 1150 2872
BsaBI GATnn'nnATC
Cuts at: 6 1536 3573 6
Size: 1530 2037 455
Fragments arranged by size:
2037 1530 455
BsaHI Gr'CG_yC
Cuts at: 1453 1701 2403 3899 1453
Size: 248 702 1496 1576
Fragments arranged by size:
1576 1496 702 248
BsaWI w'CCGG_w
Cuts at: 190 1211 1732 2410 2684 2831 3242 190
Size: 1021 521 678 274 147 411 970
Fragments arranged by size:
1021 970 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 530 551 145
Size: 385 21 3616
Fragments arranged by size:
3616 385 21
BseYI C'CCAG_C
Cuts at: 573 1447 2205 2782 3278 573
Size: 874 758 577 496 1317
Fragments arranged by size:
1317 874 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1564 1610 2818 104
Size: 1460 46 1208 1308
Fragments arranged by size:
1460 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 748 1036 1814 2004 2796 748
Size: 288 778 190 792 1974
Fragments arranged by size:
1974 792 778 288 190
BsiWI C'GTAC_G
Cuts at: 941 941
Size: 4022
Bsp1286I G_dGCh'C
Cuts at: 497 748 1036 1647 1740 1814 2004 2066 2796
Size: 251 288 611 93 74 190 62 730
Cuts at: 2796 497
Size: 1723
Fragments arranged by size:
1723 730 611 288 251 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3242 3242
Size: 4022
BspHI T'CATG_A
Cuts at: 528 3198 528
Size: 2670 1352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 878 1072 1286 1588 1969 878
Size: 194 214 302 381 2931
Fragments arranged by size:
2931 381 302 214 194
BsrBI CCG'CTC
Cuts at: 50 2314 2368 3852 4017 50
Size: 2264 54 1484 165 55
Fragments arranged by size:
2264 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1934 1934
Size: 4022
BsrFI r'CCGG_y
Cuts at: 190 2020 2201 2410 190
Size: 1830 181 209 1802
Fragments arranged by size:
1830 1802 209 181
BssSI C'ACGA_G
Cuts at: 2293 2651 2293
Size: 358 3664
BstAPI GCAn_nnn'nTGC
Cuts at: 629 879 1399 629
Size: 250 520 3252
Fragments arranged by size:
3252 520 250
BstBI TT'CG_AA
Cuts at: 2383 2383
Size: 4022
BstYI r'GATC_y
Cuts at: 1 209 353 1047 1514 1537 1872 2118 3130
Size: 208 144 694 467 23 335 246 1012
Cuts at: 3130 3216 3228 3574 1
Size: 86 12 346 449
Fragments arranged by size:
1012 694 467 449 346 335 246 208
144 86 23 12
BtgI C'CryG_G
Cuts at: 107 114 908 107
Size: 7 794 3221
Fragments arranged by size:
3221 794 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 503 1972 2153 2316 503
Size: 1469 181 163 2209
Fragments arranged by size:
2209 1469 181 163
BtsI GCAGTG_nn'
Cuts at: 1265 1265
Size: 4022
DraI TTT'AAA
Cuts at: 1021 1021
Size: 4022
DrdI GACnn_nn'nnGTC
Cuts at: 1728 2586 1728
Size: 858 3164
EaeI y'GGCC_r
Cuts at: 884 1607 1781 2172 2199 3544 884
Size: 723 174 391 27 1345 1362
Fragments arranged by size:
1362 1345 723 391 174 27
EagI C'GGCC_G
Cuts at: 1607 1607
Size: 4022
EarI CTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2230 2540 2686 3832 2230
Size: 310 146 1146 2420
Fragments arranged by size:
2420 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1034 1034
Size: 4022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1847 2279 3026 3294 1847
Size: 432 747 268 2575
Fragments arranged by size:
2575 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1161 1161
Size: 4022
EcoRI G'AATT_C
Cuts at: 1026 1026
Size: 4022
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 4022
FspI TGC'GCA
Cuts at: 1803 1803
Size: 4022
HaeII r_GCGC'y
Cuts at: 1456 1704 2726 3705 1456
Size: 248 1022 979 1773
Fragments arranged by size:
1773 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1864 1894 1896 1926 1864
Size: 30 2 30 3960
Fragments arranged by size:
3960 30 30 2
HincII GTy'rAC
Cuts at: 1061 1061
Size: 4022
HindIII A'AGCT_T
Cuts at: 562 562
Size: 4022
KasI G'GCGC_C
Cuts at: 1452 1700 1452
Size: 248 3774
KpnI G_GTAC'C
Cuts at: 1042 1042
Size: 4022
MluI A'CGCG_T
Cuts at: 3455 3455
Size: 4022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 759 2693 2877 759
Size: 1934 184 1904
Fragments arranged by size:
1934 1904 184
MscI TGG'CCA
Cuts at: 1783 1783
Size: 4022
MslI CAynn'nnrTG
Cuts at: 271 2011 2138 271
Size: 1740 127 2155
Fragments arranged by size:
2155 1740 127
MspA1I CmG'CkG
Cuts at: 116 140 1133 1447 1807 2820 3065 116
Size: 24 993 314 360 1013 245 1073
Fragments arranged by size:
1073 1013 993 360 314 245 24
NaeI GCC'GGC
Cuts at: 2203 2203
Size: 4022
NarI GG'CG_CC
Cuts at: 1453 1701 1453
Size: 248 3774
NcoI C'CATG_G
Cuts at: 107 908 107
Size: 801 3221
NdeI CA'TA_TG
Cuts at: 408 408
Size: 4022
NgoMIV G'CCGG_C
Cuts at: 2201 2201
Size: 4022
NspI r_CATG'y
Cuts at: 1075 1575 2482 1075
Size: 500 907 2615
Fragments arranged by size:
2615 907 500
PciI A'CATG_T
Cuts at: 2478 2478
Size: 4022
PfoI T'CCnGG_A
Cuts at: 431 431
Size: 4022
PmeI GTTT'AAAC
Cuts at: 1021 1021
Size: 4022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3990
PsiI TTA'TAA
Cuts at: 640 640
Size: 4022
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 943 975 943
Size: 32 3990
PstI C_TGCA'G
Cuts at: 556 830 860 875 1069 1754 556
Size: 274 30 15 194 685 2824
Fragments arranged by size:
2824 685 274 194 30 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4022
PvuII CAG'CTG
Cuts at: 140 1447 1807 140
Size: 1307 360 2355
Fragments arranged by size:
2355 1307 360
RsrII CG'GwC_CG
Cuts at: 2217 2217
Size: 4022
SacI G_AGCT'C
Cuts at: 1036 1036
Size: 4022
SacII CC_GC'GG
Cuts at: 117 117
Size: 4022
SalI G'TCGA_C
Cuts at: 1059 1059
Size: 4022
SapI GCTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
SbfI CC_TGCA'GG
Cuts at: 1069 1069
Size: 4022
ScaI AGT'ACT
Cuts at: 3539 3539
Size: 4022
SfcI C'TryA_G
Cuts at: 33 552 826 856 871 1065 1750 2743 2934
Size: 519 274 30 15 194 685 993 191
Cuts at: 2934 33
Size: 1121
Fragments arranged by size:
1121 993 685 519 274 194 191 30
15
SfoI GGC'GCC
Cuts at: 1454 1702 1454
Size: 248 3774
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
SmaI CCC'GGG
Cuts at: 1044 1044
Size: 4022
SmlI C'TyrA_G
Cuts at: 1177 2092 2584 2846 1177
Size: 915 492 262 2353
Fragments arranged by size:
2353 915 492 262
SnaBI TAC'GTA
Cuts at: 225 225
Size: 4022
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4022
SphI G_CATG'C
Cuts at: 1075 1575 1075
Size: 500 3522
SspI AAT'ATT
Cuts at: 230 230
Size: 4022
StyI C'CwwG_G
Cuts at: 107 125 692 908 1156 1510 107
Size: 18 567 216 248 354 2619
Fragments arranged by size:
2619 567 354 248 216 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 332 1605 1738 2197 2406 332
Size: 1273 133 459 209 1948
Fragments arranged by size:
1948 1273 459 209 133
TatI w'GTAC_w
Cuts at: 411 440 3537 411
Size: 29 3097 896
Fragments arranged by size:
3097 896 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3539 3571 3539
Size: 32 3990
Tth111I GACn'n_nGTC
Cuts at: 1819 1819
Size: 4022
XbaI T'CTAG_A
Cuts at: 1053 1053
Size: 4022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 906 1517 906
Size: 611 3411
XmaI C'CCGG_G
Cuts at: 1042 1042
Size: 4022
XmnI GAAnn'nnTTC
Cuts at: 148 148
Size: 4022
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PfoI PmeI PpiI PsiI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BpmI
BsaXI BsgI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PspOMI PspXI SanDI
SexAI SfiI SgrAI SrfI StuI SwaI XhoI ZraI