Restriction map for
pF1KB8175
(Circular) (Six-base) MAPSORT of: pF1KB8175.seq Check: 6728 from: 1 to: 4616
pF1KB8175 4616 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 170 170
Size: 4616
AccI GT'mk_AC
Cuts at: 1654 1654
Size: 4616
Acc65I G'GTAC_C
Cuts at: 1632 1632
Size: 4616
AclI AA'CG_TT
Cuts at: 4456 4456
Size: 4616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 583 670 1493 2441 2873 3620 3888 469
Size: 114 87 823 948 432 747 268 1197
Fragments arranged by size:
1197 948 823 747 432 268 114 87
AflIII A'CryG_T
Cuts at: 1968 3072 4049 1968
Size: 1104 977 2535
Fragments arranged by size:
2535 1104 977
AgeI A'CCGG_T
Cuts at: 3004 3004
Size: 4616
AlwNI CAG_nnn'CTG
Cuts at: 1921 3488 1921
Size: 1567 3049
ApaI G_GGCC'C
Cuts at: 1364 1364
Size: 4616
ApaLI G'TGCA_C
Cuts at: 3386 3386
Size: 4616
ApoI r'AATT_y
Cuts at: 355 385 433 1620 3969 4225 355
Size: 30 48 1187 2349 256 746
Fragments arranged by size:
2349 1187 746 256 48 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4616
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
AvaI C'yCGr_G
Cuts at: 236 455 1108 1636 236
Size: 219 653 528 3216
Fragments arranged by size:
3216 653 528 219
BamHI G'GATC_C
Cuts at: 1641 1641
Size: 4616
BanI G'GyrC_C
Cuts at: 1033 1632 2046 2294 2329 1033
Size: 599 414 248 35 3320
Fragments arranged by size:
3320 599 414 248 35
BanII G_rGCy'C
Cuts at: 323 834 954 1364 1630 2660 323
Size: 511 120 410 266 1030 2279
Fragments arranged by size:
2279 1030 511 410 266 120
BbeI G_GCGC'C
Cuts at: 2050 2298 2050
Size: 248 4368
BbsI GAAGACnn'nnnn_
Cuts at: 1553 1582 1553
Size: 29 4587
BciVI GTATCCnnnnn_n'
Cuts at: 2514 3275 4616 2514
Size: 761 1341 2514
Fragments arranged by size:
2514 1341 761
BclI T'GATC_A
Cuts at: 225 2136 225
Size: 1911 2705
BglII A'GATC_T
Cuts at: 410 1085 2131 4168 410
Size: 675 1046 2037 858
Fragments arranged by size:
2037 1046 858 675
BlpI GC'TnA_GC
Cuts at: 1728 1728
Size: 4616
Bme1580I G_kGCm'C
Cuts at: 816 1036 1364 2241 2334 3390 816
Size: 220 328 877 93 1056 2042
Fragments arranged by size:
2042 1056 877 328 220 93
BmrI ACTGGGnnnn_n'
Cuts at: 1828 1909 2244 4388 1828
Size: 81 335 2144 2056
Fragments arranged by size:
2144 2056 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 4616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 848 1006 142
Size: 706 158 3752
Fragments arranged by size:
3752 706 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 532 697 1536 1792 2671 3163 3461 355
Size: 177 165 839 256 879 492 298 1510
Fragments arranged by size:
1510 879 839 492 298 256 177 165
BsaI GGTCTCn'nnnn_
Cuts at: 4320 4320
Size: 4616
BsaAI yAC'GTr
Cuts at: 1969 1969
Size: 4616
BsaBI GATnn'nnATC
Cuts at: 6 897 2130 4167 6
Size: 891 1233 2037 455
Fragments arranged by size:
2037 1233 891 455
BsaHI Gr'CG_yC
Cuts at: 167 1395 2047 2295 2997 4493 167
Size: 1228 652 248 702 1496 290
Fragments arranged by size:
1496 1228 702 652 290 248
BsaWI w'CCGG_w
Cuts at: 392 1805 2326 3004 3278 3425 3836 392
Size: 1413 521 678 274 147 411 1172
Fragments arranged by size:
1413 1172 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 331 846 880 160
Size: 171 515 34 3896
Fragments arranged by size:
3896 515 171 34
BseYI C'CCAG_C
Cuts at: 1265 2041 2799 3376 3872 1265
Size: 776 758 577 496 2009
Fragments arranged by size:
2009 776 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2158 2204 3412 104
Size: 2054 46 1208 1308
Fragments arranged by size:
2054 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 323 1630 2408 2598 3390 323
Size: 1307 778 190 792 1549
Fragments arranged by size:
1549 1307 792 778 190
BsmI GAATG_Cn'
Cuts at: 648 850 648
Size: 202 4414
Bsp1286I G_dGCh'C
Cuts at: 323 816 834 954 1036 1364 1630 2241 2334
Size: 493 18 120 82 328 266 611 93
Cuts at: 2334 2408 2598 2660 3390 323
Size: 74 190 62 730 1549
Fragments arranged by size:
1549 730 611 493 328 266 190 120
93 82 74 62 18
BspEI T'CCGG_A
Cuts at: 3836 3836
Size: 4616
BspHI T'CATG_A
Cuts at: 141 1131 3792 141
Size: 990 2661 965
Fragments arranged by size:
2661 990 965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1262 1666 1880 2182 2563 1262
Size: 404 214 302 381 3315
Fragments arranged by size:
3315 404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2908 2962 4446 4611 50
Size: 2858 54 1484 165 55
Fragments arranged by size:
2858 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1064 2528 1064
Size: 1464 3152
BsrFI r'CCGG_y
Cuts at: 2614 2795 3004 2614
Size: 181 209 4226
Fragments arranged by size:
4226 209 181
BsrGI T'GTAC_A
Cuts at: 1140 1140
Size: 4616
BssSI C'ACGA_G
Cuts at: 2887 3245 2887
Size: 358 4258
BstAPI GCAn_nnn'nTGC
Cuts at: 1993 1993
Size: 4616
BstBI TT'CG_AA
Cuts at: 2977 2977
Size: 4616
BstXI CCAn_nnnn'nTGG
Cuts at: 1265 1265
Size: 4616
BstYI r'GATC_y
Cuts at: 1 410 1085 1587 1641 2108 2131 2466 2712
Size: 409 675 502 54 467 23 335 246
Cuts at: 2712 3724 3810 3822 4168 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 675 502 467 449 409 346 335
246 86 54 23 12
Bsu36I CC'TnA_GG
Cuts at: 277 277
Size: 4616
BtgI C'CryG_G
Cuts at: 107 107
Size: 4616
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2566 2747 2910 2566
Size: 181 163 4272
Fragments arranged by size:
4272 181 163
BtsI GCAGTG_nn'
Cuts at: 816 1433 1859 816
Size: 617 426 3573
Fragments arranged by size:
3573 617 426
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 410 445 410
Size: 35 4581
DraI TTT'AAA
Cuts at: 1615 1615
Size: 4616
DrdI GACnn_nn'nnGTC
Cuts at: 2322 3180 2322
Size: 858 3758
EaeI y'GGCC_r
Cuts at: 793 2201 2375 2766 2793 4138 793
Size: 1408 174 391 27 1345 1271
Fragments arranged by size:
1408 1345 1271 391 174 27
EagI C'GGCC_G
Cuts at: 2201 2201
Size: 4616
EarI CTCTTCn'nnn_
Cuts at: 467 500 938 1163 1175 1184 1217 2639 2849
Size: 33 438 225 12 9 33 1422 210
Cuts at: 2849 467
Size: 2234
Fragments arranged by size:
2234 1422 438 225 210 33 33 12
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2824 3134 3280 4426 2824
Size: 310 146 1146 3014
Fragments arranged by size:
3014 1146 310 146
EcoICRI GAG'CTC
Cuts at: 321 1628 321
Size: 1307 3309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 469 583 670 848 1006 1493 2441 2873
Size: 327 114 87 178 158 487 948 432
Cuts at: 2873 3620 3888 142
Size: 747 268 870
Fragments arranged by size:
948 870 747 487 432 327 268 178
158 114 87
EcoO109I rG'GnC_Cy
Cuts at: 337 779 935 1360 1755 337
Size: 442 156 425 395 3198
Fragments arranged by size:
3198 442 425 395 156
EcoRI G'AATT_C
Cuts at: 355 385 1620 355
Size: 30 1235 3351
Fragments arranged by size:
3351 1235 30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 4616
FspI TGC'GCA
Cuts at: 2397 2397
Size: 4616
HaeII r_GCGC'y
Cuts at: 138 2050 2298 3320 4299 138
Size: 1912 248 1022 979 455
Fragments arranged by size:
1912 1022 979 455 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1459 1491 2458 2488 2490 2520 1459
Size: 32 967 30 2 30 3555
Fragments arranged by size:
3555 967 32 30 30 2
HincII GTy'rAC
Cuts at: 1655 1655
Size: 4616
HindIII A'AGCT_T
Cuts at: 1300 1300
Size: 4616
KasI G'GCGC_C
Cuts at: 2046 2294 2046
Size: 248 4368
KpnI G_GTAC'C
Cuts at: 1636 1636
Size: 4616
MluI A'CGCG_T
Cuts at: 4049 4049
Size: 4616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3287 3471 3287
Size: 184 4432
MscI TGG'CCA
Cuts at: 2377 2377
Size: 4616
MslI CAynn'nnrTG
Cuts at: 404 1136 1403 2605 2732 404
Size: 732 267 1202 127 2288
Fragments arranged by size:
2288 1202 732 267 127
MspA1I CmG'CkG
Cuts at: 132 1727 2041 2401 3414 3659 132
Size: 1595 314 360 1013 245 1089
Fragments arranged by size:
1595 1089 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2797 2797
Size: 4616
NarI GG'CG_CC
Cuts at: 2047 2295 2047
Size: 248 4368
NcoI C'CATG_G
Cuts at: 107 107
Size: 4616
NgoMIV G'CCGG_C
Cuts at: 2795 2795
Size: 4616
NspI r_CATG'y
Cuts at: 1669 2169 3076 1669
Size: 500 907 3209
Fragments arranged by size:
3209 907 500
PciI A'CATG_T
Cuts at: 3072 3072
Size: 4616
PfoI T'CCnGG_A
Cuts at: 206 786 206
Size: 580 4036
PmeI GTTT'AAAC
Cuts at: 1615 1615
Size: 4616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4584
PpuMI rG'GwC_Cy
Cuts at: 337 779 935 337
Size: 442 156 4018
Fragments arranged by size:
4018 442 156
PspOMI G'GGCC_C
Cuts at: 1360 1360
Size: 4616
PstI C_TGCA'G
Cuts at: 1663 2348 1663
Size: 685 3931
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4616
PvuII CAG'CTG
Cuts at: 2041 2401 2041
Size: 360 4256
RsrII CG'GwC_CG
Cuts at: 2811 2811
Size: 4616
SacI G_AGCT'C
Cuts at: 323 1630 323
Size: 1307 3309
SalI G'TCGA_C
Cuts at: 1653 1653
Size: 4616
SanDI GG'GwC_CC
Cuts at: 935 935
Size: 4616
SapI GCTCTTCn'nnn_
Cuts at: 938 1163 1184 2639 2849 938
Size: 225 21 1455 210 2705
Fragments arranged by size:
2705 1455 225 210 21
SbfI CC_TGCA'GG
Cuts at: 1663 1663
Size: 4616
ScaI AGT'ACT
Cuts at: 4133 4133
Size: 4616
SfcI C'TryA_G
Cuts at: 33 1659 2344 3337 3528 33
Size: 1626 685 993 191 1121
Fragments arranged by size:
1626 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2048 2296 2048
Size: 248 4368
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
SmaI CCC'GGG
Cuts at: 1638 1638
Size: 4616
SmlI C'TyrA_G
Cuts at: 370 455 547 676 1551 1771 2686 3178 3440
Size: 85 92 129 875 220 915 492 262
Cuts at: 3440 370
Size: 1546
Fragments arranged by size:
1546 915 875 492 262 220 129 92
85
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4616
SphI G_CATG'C
Cuts at: 1669 2169 1669
Size: 500 4116
StyI C'CwwG_G
Cuts at: 107 1750 2104 107
Size: 1643 354 2619
Fragments arranged by size:
2619 1643 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 416 2199 2332 2791 3000 416
Size: 1783 133 459 209 2032
Fragments arranged by size:
2032 1783 459 209 133
TatI w'GTAC_w
Cuts at: 551 1140 4131 551
Size: 589 2991 1036
Fragments arranged by size:
2991 1036 589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4133 4165 4133
Size: 32 4584
Tth111I GACn'n_nGTC
Cuts at: 2413 2413
Size: 4616
XbaI T'CTAG_A
Cuts at: 1074 1647 1074
Size: 573 4043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 2111
Size: 4616
XhoI C'TCGA_G
Cuts at: 455 455
Size: 4616
XmaI C'CCGG_G
Cuts at: 1636 1636
Size: 4616
ZraI GAC'GTC
Cuts at: 168 168
Size: 4616
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII BstZ17I
ClaI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI