Restriction map for
pF1KB8171
(Circular) (Six-base) MAPSORT of: pF1KB8171.seq Check: 1383 from: 1 to: 4784
pF1KB8171 4784 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 322 322
Size: 4784
AccI GT'mk_AC
Cuts at: 1822 1822
Size: 4784
Acc65I G'GTAC_C
Cuts at: 1800 1800
Size: 4784
AclI AA'CG_TT
Cuts at: 4624 4624
Size: 4784
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 523 569 606 1521 1614 1703 2609 3041
Size: 81 46 37 915 93 89 906 432
Cuts at: 3041 3788 4056 442
Size: 747 268 1170
Fragments arranged by size:
1170 915 906 747 432 268 93 89
81 46 37
AflII C'TTAA_G
Cuts at: 714 714
Size: 4784
AflIII A'CryG_T
Cuts at: 2136 3240 4217 2136
Size: 1104 977 2703
Fragments arranged by size:
2703 1104 977
AgeI A'CCGG_T
Cuts at: 3172 3172
Size: 4784
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1065 1066 1097 1098 1583 1615 1065
Size: 1 31 1 485 32 4234
Fragments arranged by size:
4234 485 32 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 170 378 681 952 1554 2089 3656 170
Size: 208 303 271 602 535 1567 1298
Fragments arranged by size:
1567 1298 602 535 303 271 208
ApaLI G'TGCA_C
Cuts at: 453 685 3554 453
Size: 232 2869 1683
Fragments arranged by size:
2869 1683 232
ApoI r'AATT_y
Cuts at: 647 750 838 1277 1788 4137 4393 647
Size: 103 88 439 511 2349 256 1038
Fragments arranged by size:
2349 1038 511 439 256 103 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 4784
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
AvaI C'yCGr_G
Cuts at: 1466 1804 1466
Size: 338 4446
BamHI G'GATC_C
Cuts at: 1809 1809
Size: 4784
BanI G'GyrC_C
Cuts at: 1198 1800 2214 2462 2497 1198
Size: 602 414 248 35 3485
Fragments arranged by size:
3485 602 414 248 35
BanII G_rGCy'C
Cuts at: 1798 2828 1798
Size: 1030 3754
BbeI G_GCGC'C
Cuts at: 1202 2218 2466 1202
Size: 1016 248 3520
Fragments arranged by size:
3520 1016 248
BbsI GAAGACnn'nnnn_
Cuts at: 1262 1303 1262
Size: 41 4743
BciVI GTATCCnnnnn_n'
Cuts at: 1199 2682 3443 4784 1199
Size: 1483 761 1341 1199
Fragments arranged by size:
1483 1341 1199 761
BclI T'GATC_A
Cuts at: 2304 2304
Size: 4784
BfrBI ATG'CAT
Cuts at: 987 987
Size: 4784
BglII A'GATC_T
Cuts at: 2299 4336 2299
Size: 2037 2747
BlpI GC'TnA_GC
Cuts at: 1896 1896
Size: 4784
Bme1580I G_kGCm'C
Cuts at: 457 689 1628 2409 2502 3558 457
Size: 232 939 781 93 1056 1683
Fragments arranged by size:
1683 1056 939 781 232 93
BmrI ACTGGGnnnn_n'
Cuts at: 160 319 813 1996 2077 2412 4556 160
Size: 159 494 1183 81 335 2144 388
Fragments arranged by size:
2144 1183 494 388 335 159 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3151 3151
Size: 4784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1196 1637 1196
Size: 441 4343
Bpu10I CC'TnA_GC
Cuts at: 1316 1316
Size: 4784
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1960 2839 3331 3629 241
Size: 1719 879 492 298 1396
Fragments arranged by size:
1719 1396 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 761 1230 4488 761
Size: 469 3258 1057
Fragments arranged by size:
3258 1057 469
BsaAI yAC'GTr
Cuts at: 2137 2137
Size: 4784
BsaBI GATnn'nnATC
Cuts at: 6 2298 4335 6
Size: 2292 2037 455
Fragments arranged by size:
2292 2037 455
BsaHI Gr'CG_yC
Cuts at: 1199 2215 2463 3165 4661 1199
Size: 1016 248 702 1496 1322
Fragments arranged by size:
1496 1322 1016 702 248
BsaWI w'CCGG_w
Cuts at: 1973 2494 3172 3446 3593 4004 1973
Size: 521 678 274 147 411 2753
Fragments arranged by size:
2753 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 793 823 1583 1613 793
Size: 30 760 30 3964
Fragments arranged by size:
3964 760 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 881 379
Size: 502 4282
BseYI C'CCAG_C
Cuts at: 720 2209 2967 3544 4040 720
Size: 1489 758 577 496 1464
Fragments arranged by size:
1489 1464 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 910 1292 391
Size: 519 382 3883
Fragments arranged by size:
3883 519 382
BsiEI CG_ry'CG
Cuts at: 104 2326 2372 3580 104
Size: 2222 46 1208 1308
Fragments arranged by size:
2222 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 457 689 1798 2576 2766 3558 457
Size: 232 1109 778 190 792 1683
Fragments arranged by size:
1683 1109 792 778 232 190
BsmI GAATG_Cn'
Cuts at: 586 987 586
Size: 401 4383
Bsp1286I G_dGCh'C
Cuts at: 457 689 1628 1798 2409 2502 2576 2766 2828
Size: 232 939 170 611 93 74 190 62
Cuts at: 2828 3558 457
Size: 730 1683
Fragments arranged by size:
1683 939 730 611 232 190 170 93
74 62
BspEI T'CCGG_A
Cuts at: 4004 4004
Size: 4784
BspHI T'CATG_A
Cuts at: 1230 3960 1230
Size: 2730 2054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 322 1834 2048 2350 2731 322
Size: 1512 214 302 381 2375
Fragments arranged by size:
2375 1512 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3076 3130 4614 4779 50
Size: 3026 54 1484 165 55
Fragments arranged by size:
3026 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 570 878 1343 2696 570
Size: 308 465 1353 2658
Fragments arranged by size:
2658 1353 465 308
BsrFI r'CCGG_y
Cuts at: 779 2782 2963 3172 779
Size: 2003 181 209 2391
Fragments arranged by size:
2391 2003 209 181
BssSI C'ACGA_G
Cuts at: 3055 3413 3055
Size: 358 4426
BstAPI GCAn_nnn'nTGC
Cuts at: 378 452 592 684 2161 378
Size: 74 140 92 1477 3001
Fragments arranged by size:
3001 1477 140 92 74
BstBI TT'CG_AA
Cuts at: 3145 3145
Size: 4784
BstEII G'GTnAC_C
Cuts at: 1580 1580
Size: 4784
BstXI CCAn_nnnn'nTGG
Cuts at: 150 150
Size: 4784
BstYI r'GATC_y
Cuts at: 1 1809 2276 2299 2634 2880 3892 3978 3990
Size: 1808 467 23 335 246 1012 86 12
Cuts at: 3990 4336 1
Size: 346 449
Fragments arranged by size:
1808 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1391 2734 2915 3078 1391
Size: 1343 181 163 3097
Fragments arranged by size:
3097 1343 181 163
BtsI GCAGTG_nn'
Cuts at: 283 898 1049 2027 283
Size: 615 151 978 3040
Fragments arranged by size:
3040 978 615 151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1326 1361 1326
Size: 35 4749
DraI TTT'AAA
Cuts at: 1783 1783
Size: 4784
DrdI GACnn_nn'nnGTC
Cuts at: 247 2490 3348 247
Size: 2243 858 1683
Fragments arranged by size:
2243 1683 858
EaeI y'GGCC_r
Cuts at: 462 1457 2369 2543 2934 2961 4306 462
Size: 995 912 174 391 27 1345 940
Fragments arranged by size:
1345 995 940 912 391 174 27
EagI C'GGCC_G
Cuts at: 2369 2369
Size: 4784
EarI CTCTTCn'nnn_
Cuts at: 2807 3017 2807
Size: 210 4574
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2992 3302 3448 4594 2992
Size: 310 146 1146 3182
Fragments arranged by size:
3182 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1796 1796
Size: 4784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 523 569 606 1196 1521 1614 1637 1703
Size: 81 46 37 590 325 93 23 66
Cuts at: 1703 2609 3041 3788 4056 442
Size: 906 432 747 268 1170
Fragments arranged by size:
1170 906 747 590 432 325 268 93
81 66 46 37 23
EcoO109I rG'GnC_Cy
Cuts at: 1065 1923 1065
Size: 858 3926
EcoRI G'AATT_C
Cuts at: 838 1277 1788 838
Size: 439 511 3834
Fragments arranged by size:
3834 511 439
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 636 843 2803 636
Size: 207 1960 2617
Fragments arranged by size:
2617 1960 207
FspI TGC'GCA
Cuts at: 2565 2565
Size: 4784
HaeII r_GCGC'y
Cuts at: 378 1202 2218 2466 3488 4467 378
Size: 824 1016 248 1022 979 695
Fragments arranged by size:
1022 1016 979 824 695 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 252 284 793 825 948 980 1598 1630 2626
Size: 32 509 32 123 32 618 32 996
Cuts at: 2626 2656 2658 2688 252
Size: 30 2 30 2348
Fragments arranged by size:
2348 996 618 509 123 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 291 1823 291
Size: 1532 3252
HindIII A'AGCT_T
Cuts at: 1127 1127
Size: 4784
KasI G'GCGC_C
Cuts at: 1198 2214 2462 1198
Size: 1016 248 3520
Fragments arranged by size:
3520 1016 248
KpnI G_GTAC'C
Cuts at: 1804 1804
Size: 4784
MluI A'CGCG_T
Cuts at: 4217 4217
Size: 4784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1347 3455 3639 1347
Size: 2108 184 2492
Fragments arranged by size:
2492 2108 184
MscI TGG'CCA
Cuts at: 464 2545 464
Size: 2081 2703
MslI CAynn'nnrTG
Cuts at: 538 1453 1513 1549 2773 2900 538
Size: 915 60 36 1224 127 2422
Fragments arranged by size:
2422 1224 915 127 60 36
MspA1I CmG'CkG
Cuts at: 139 681 764 1079 1657 1895 2209 2569 3582
Size: 542 83 315 578 238 314 360 1013
Cuts at: 3582 3827 139
Size: 245 1096
Fragments arranged by size:
1096 1013 578 542 360 315 314 245
238 83
NaeI GCC'GGC
Cuts at: 781 2965 781
Size: 2184 2600
NarI GG'CG_CC
Cuts at: 1199 2215 2463 1199
Size: 1016 248 3520
Fragments arranged by size:
3520 1016 248
NgoMIV G'CCGG_C
Cuts at: 779 2963 779
Size: 2184 2600
NsiI A_TGCA'T
Cuts at: 989 989
Size: 4784
NspI r_CATG'y
Cuts at: 1548 1837 2337 3244 1548
Size: 289 500 907 3088
Fragments arranged by size:
3088 907 500 289
PciI A'CATG_T
Cuts at: 3240 3240
Size: 4784
PflMI CCAn_nnn'nTGG
Cuts at: 1701 1701
Size: 4784
PmeI GTTT'AAAC
Cuts at: 1783 1783
Size: 4784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1583 1615 3923 3955 1583
Size: 32 2308 32 2412
Fragments arranged by size:
2412 2308 32 32
PpuMI rG'GwC_Cy
Cuts at: 1065 1065
Size: 4784
PstI C_TGCA'G
Cuts at: 1831 2516 1831
Size: 685 4099
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4784
PvuII CAG'CTG
Cuts at: 681 1079 1657 2209 2569 681
Size: 398 578 552 360 2896
Fragments arranged by size:
2896 578 552 398 360
RsrII CG'GwC_CG
Cuts at: 2979 2979
Size: 4784
SacI G_AGCT'C
Cuts at: 1798 1798
Size: 4784
SalI G'TCGA_C
Cuts at: 1821 1821
Size: 4784
SanDI GG'GwC_CC
Cuts at: 1065 1065
Size: 4784
SapI GCTCTTCn'nnn_
Cuts at: 2807 3017 2807
Size: 210 4574
SbfI CC_TGCA'GG
Cuts at: 1831 1831
Size: 4784
ScaI AGT'ACT
Cuts at: 4301 4301
Size: 4784
SfcI C'TryA_G
Cuts at: 33 1040 1827 2512 3505 3696 33
Size: 1007 787 685 993 191 1121
Fragments arranged by size:
1121 1007 993 787 685 191
SfoI GGC'GCC
Cuts at: 1200 2216 2464 1200
Size: 1016 248 3520
Fragments arranged by size:
3520 1016 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
SmaI CCC'GGG
Cuts at: 1468 1806 1468
Size: 338 4446
SmlI C'TyrA_G
Cuts at: 256 714 1939 2854 3346 3608 256
Size: 458 1225 915 492 262 1432
Fragments arranged by size:
1432 1225 915 492 458 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4784
SphI G_CATG'C
Cuts at: 1837 2337 1837
Size: 500 4284
StyI C'CwwG_G
Cuts at: 438 1397 1586 1918 2272 438
Size: 959 189 332 354 2950
Fragments arranged by size:
2950 959 354 332 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1000 1599 2367 2500 2959 3168 1000
Size: 599 768 133 459 209 2616
Fragments arranged by size:
2616 768 599 459 209 133
TatI w'GTAC_w
Cuts at: 329 914 1217 4299 329
Size: 585 303 3082 814
Fragments arranged by size:
3082 814 585 303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4301 4333 4301
Size: 32 4752
Tth111I GACn'n_nGTC
Cuts at: 1612 2581 1612
Size: 969 3815
XbaI T'CTAG_A
Cuts at: 1815 1815
Size: 4784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2279 2279
Size: 4784
XmaI C'CCGG_G
Cuts at: 1466 1804 1466
Size: 338 4446
XmnI GAAnn'nnTTC
Cuts at: 650 733 650
Size: 83 4701
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I BtgI ClaI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI