Restriction map for
pF1KB8167
(Circular) (Six-base) MAPSORT of: pF1KB8167.seq Check: 7956 from: 1 to: 4430
pF1KB8167 4430 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 151 1172 1468 151
Size: 1021 296 3113
Fragments arranged by size:
3113 1021 296
Acc65I G'GTAC_C
Cuts at: 1446 1446
Size: 4430
AclI AA'CG_TT
Cuts at: 4270 4270
Size: 4430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 2255 2687 3434 3702 458
Size: 1797 432 747 268 1186
Fragments arranged by size:
1797 1186 747 432 268
AfeI AGC'GCT
Cuts at: 123 995 1191 123
Size: 872 196 3362
Fragments arranged by size:
3362 872 196
AflII C'TTAA_G
Cuts at: 703 703
Size: 4430
AflIII A'CryG_T
Cuts at: 1782 2886 3863 1782
Size: 1104 977 2349
Fragments arranged by size:
2349 1104 977
AgeI A'CCGG_T
Cuts at: 2818 2818
Size: 4430
AleI CACnn'nnGTG
Cuts at: 1139 1139
Size: 4430
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 862 894 862
Size: 32 4398
AlwNI CAG_nnn'CTG
Cuts at: 325 655 1735 3302 325
Size: 330 1080 1567 1453
Fragments arranged by size:
1567 1453 1080 330
ApaLI G'TGCA_C
Cuts at: 3200 3200
Size: 4430
ApoI r'AATT_y
Cuts at: 283 1434 3783 4039 283
Size: 1151 2349 256 674
Fragments arranged by size:
2349 1151 674 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4430
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
AvaI C'yCGr_G
Cuts at: 1450 1450
Size: 4430
BamHI G'GATC_C
Cuts at: 1455 1455
Size: 4430
BanI G'GyrC_C
Cuts at: 1141 1446 1860 2108 2143 1141
Size: 305 414 248 35 3428
Fragments arranged by size:
3428 414 305 248 35
BanII G_rGCy'C
Cuts at: 1444 2474 1444
Size: 1030 3400
BbeI G_GCGC'C
Cuts at: 1864 2112 1864
Size: 248 4182
BbsI GAAGACnn'nnnn_
Cuts at: 503 705 503
Size: 202 4228
BbvCI CC'TCA_GC
Cuts at: 806 806
Size: 4430
BciVI GTATCCnnnnn_n'
Cuts at: 2328 3089 4430 2328
Size: 761 1341 2328
Fragments arranged by size:
2328 1341 761
BclI T'GATC_A
Cuts at: 1950 1950
Size: 4430
BglII A'GATC_T
Cuts at: 1945 3982 1945
Size: 2037 2393
BlpI GC'TnA_GC
Cuts at: 1542 1542
Size: 4430
Bme1580I G_kGCm'C
Cuts at: 1341 2055 2148 3204 1341
Size: 714 93 1056 2567
Fragments arranged by size:
2567 1056 714 93
BmrI ACTGGGnnnn_n'
Cuts at: 163 1642 1723 2058 4202 163
Size: 1479 81 335 2144 391
Fragments arranged by size:
2144 1479 391 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 428 474 2797 428
Size: 46 2323 2061
Fragments arranged by size:
2323 2061 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 802 1222 653
Size: 149 420 3861
Fragments arranged by size:
3861 420 149
Bpu10I CC'TnA_GC
Cuts at: 806 1263 806
Size: 457 3973
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1018 1606 2485 2977 3275 814
Size: 204 588 879 492 298 1969
Fragments arranged by size:
1969 879 588 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 4134 4134
Size: 4430
BsaAI yAC'GTr
Cuts at: 1783 1783
Size: 4430
BsaBI GATnn'nnATC
Cuts at: 6 1944 3981 6
Size: 1938 2037 455
Fragments arranged by size:
2037 1938 455
BsaHI Gr'CG_yC
Cuts at: 1861 2109 2811 4307 1861
Size: 248 702 1496 1984
Fragments arranged by size:
1984 1496 702 248
BsaWI w'CCGG_w
Cuts at: 229 1619 2140 2818 3092 3239 3650 229
Size: 1390 521 678 274 147 411 1009
Fragments arranged by size:
1390 1009 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 352 529 532 652 940 1065 286
Size: 66 177 3 120 288 125 3651
Fragments arranged by size:
3651 288 177 125 120 66 3
BseYI C'CCAG_C
Cuts at: 157 1372 1855 2613 3190 3686 157
Size: 1215 483 758 577 496 901
Fragments arranged by size:
1215 901 758 577 496 483
BsiEI CG_ry'CG
Cuts at: 104 1972 2018 3226 104
Size: 1868 46 1208 1308
Fragments arranged by size:
1868 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 576 1444 2222 2412 3204 576
Size: 868 778 190 792 1802
Fragments arranged by size:
1802 868 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 576 1341 1444 2055 2148 2222 2412 2474 3204
Size: 765 103 611 93 74 190 62 730
Cuts at: 3204 576
Size: 1802
Fragments arranged by size:
1802 765 730 611 190 103 93 74
62
BspEI T'CCGG_A
Cuts at: 229 3650 229
Size: 3421 1009
BspHI T'CATG_A
Cuts at: 128 3606 128
Size: 3478 952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1233 1480 1694 1996 2377 1233
Size: 247 214 302 381 3286
Fragments arranged by size:
3286 381 302 247 214
BsrBI CCG'CTC
Cuts at: 50 546 1395 2722 2776 4260 4425 50
Size: 496 849 1327 54 1484 165 55
Fragments arranged by size:
1484 1327 849 496 165 55 54
BsrDI GCAATG_nn'
Cuts at: 183 2342 183
Size: 2159 2271
BsrFI r'CCGG_y
Cuts at: 2428 2609 2818 2428
Size: 181 209 4040
Fragments arranged by size:
4040 209 181
BssSI C'ACGA_G
Cuts at: 2701 3059 2701
Size: 358 4072
BstAPI GCAn_nnn'nTGC
Cuts at: 895 1807 895
Size: 912 3518
BstBI TT'CG_AA
Cuts at: 720 2791 720
Size: 2071 2359
BstYI r'GATC_y
Cuts at: 1 1455 1922 1945 2280 2526 3538 3624 3636
Size: 1454 467 23 335 246 1012 86 12
Cuts at: 3636 3982 1
Size: 346 449
Fragments arranged by size:
1454 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1173 1173
Size: 4430
Bsu36I CC'TnA_GG
Cuts at: 326 326
Size: 4430
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2380 2561 2724 2380
Size: 181 163 4086
Fragments arranged by size:
4086 181 163
BtsI GCAGTG_nn'
Cuts at: 510 1673 510
Size: 1163 3267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1293 1328 1293
Size: 35 4395
DraI TTT'AAA
Cuts at: 1429 1429
Size: 4430
DraIII CAC_nnn'GTG
Cuts at: 1290 1290
Size: 4430
DrdI GACnn_nn'nnGTC
Cuts at: 1265 2136 2994 1265
Size: 871 858 2701
Fragments arranged by size:
2701 871 858
EaeI y'GGCC_r
Cuts at: 1396 2015 2189 2580 2607 3952 1396
Size: 619 174 391 27 1345 1874
Fragments arranged by size:
1874 1345 619 391 174 27
EagI C'GGCC_G
Cuts at: 2015 2015
Size: 4430
EarI CTCTTCn'nnn_
Cuts at: 395 506 587 659 836 860 1040 2453 2663
Size: 111 81 72 177 24 180 1413 210
Cuts at: 2663 395
Size: 2162
Fragments arranged by size:
2162 1413 210 180 177 111 81 72
24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2638 2948 3094 4240 2638
Size: 310 146 1146 2828
Fragments arranged by size:
2828 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1442 1442
Size: 4430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 653 802 1222 2255 2687 3434 3702 458
Size: 195 149 420 1033 432 747 268 1186
Fragments arranged by size:
1186 1033 747 432 420 268 195 149
EcoNI CCTnn'n_nnAGG
Cuts at: 1039 1039
Size: 4430
EcoO109I rG'GnC_Cy
Cuts at: 1569 1569
Size: 4430
EcoRI G'AATT_C
Cuts at: 1434 1434
Size: 4430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 729 2449 729
Size: 1720 2710
FspI TGC'GCA
Cuts at: 2211 2211
Size: 4430
HaeII r_GCGC'y
Cuts at: 125 997 1193 1864 2112 3134 4113 125
Size: 872 196 671 248 1022 979 442
Fragments arranged by size:
1022 979 872 671 442 248 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 331 363 631 663 681 713 1291 1323 1384
Size: 32 268 32 18 32 578 32 61
Cuts at: 1384 1416 2272 2302 2304 2334 331
Size: 32 856 30 2 30 2427
Fragments arranged by size:
2427 856 578 268 61 32 32 32
32 32 30 30 18 2
HincII GTy'rAC
Cuts at: 1469 1469
Size: 4430
KasI G'GCGC_C
Cuts at: 1860 2108 1860
Size: 248 4182
KpnI G_GTAC'C
Cuts at: 1450 1450
Size: 4430
MluI A'CGCG_T
Cuts at: 3863 3863
Size: 4430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3101 3285 3101
Size: 184 4246
MscI TGG'CCA
Cuts at: 2191 2191
Size: 4430
MslI CAynn'nnrTG
Cuts at: 610 1139 2419 2546 610
Size: 529 1280 127 2494
Fragments arranged by size:
2494 1280 529 127
MspA1I CmG'CkG
Cuts at: 1372 1541 1855 2215 3228 3473 1372
Size: 169 314 360 1013 245 2329
Fragments arranged by size:
2329 1013 360 314 245 169
NaeI GCC'GGC
Cuts at: 2611 2611
Size: 4430
NarI GG'CG_CC
Cuts at: 1861 2109 1861
Size: 248 4182
NgoMIV G'CCGG_C
Cuts at: 2609 2609
Size: 4430
NspI r_CATG'y
Cuts at: 1029 1483 1983 2890 1029
Size: 454 500 907 2569
Fragments arranged by size:
2569 907 500 454
PciI A'CATG_T
Cuts at: 2886 2886
Size: 4430
PmeI GTTT'AAAC
Cuts at: 1429 1429
Size: 4430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 725 757 3569 3601 725
Size: 32 2812 32 1554
Fragments arranged by size:
2812 1554 32 32
PstI C_TGCA'G
Cuts at: 1477 2162 1477
Size: 685 3745
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4430
PvuII CAG'CTG
Cuts at: 1372 1855 2215 1372
Size: 483 360 3587
Fragments arranged by size:
3587 483 360
RsrII CG'GwC_CG
Cuts at: 2625 2625
Size: 4430
SacI G_AGCT'C
Cuts at: 1444 1444
Size: 4430
SalI G'TCGA_C
Cuts at: 1467 1467
Size: 4430
SapI GCTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4220
SbfI CC_TGCA'GG
Cuts at: 1477 1477
Size: 4430
ScaI AGT'ACT
Cuts at: 3947 3947
Size: 4430
SfcI C'TryA_G
Cuts at: 33 1473 2158 3151 3342 33
Size: 1440 685 993 191 1121
Fragments arranged by size:
1440 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1862 2110 1862
Size: 248 4182
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
SmaI CCC'GGG
Cuts at: 1452 1452
Size: 4430
SmlI C'TyrA_G
Cuts at: 703 829 997 1585 2500 2992 3254 703
Size: 126 168 588 915 492 262 1879
Fragments arranged by size:
1879 915 588 492 262 168 126
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4430
SphI G_CATG'C
Cuts at: 1029 1483 1983 1029
Size: 454 500 3476
Fragments arranged by size:
3476 500 454
StyI C'CwwG_G
Cuts at: 1564 1918 1564
Size: 354 4076
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 102 746 2013 2146 2605 2814 102
Size: 644 1267 133 459 209 1718
Fragments arranged by size:
1718 1267 644 459 209 133
TatI w'GTAC_w
Cuts at: 668 821 3945 668
Size: 153 3124 1153
Fragments arranged by size:
3124 1153 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3947 3979 3947
Size: 32 4398
Tth111I GACn'n_nGTC
Cuts at: 2227 2227
Size: 4430
XbaI T'CTAG_A
Cuts at: 1461 1461
Size: 4430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 858 1925 858
Size: 1067 3363
XmaI C'CCGG_G
Cuts at: 1450 1450
Size: 4430
XmnI GAAnn'nnTTC
Cuts at: 284 284
Size: 4430
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AhdI AlfI ApaI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BtgI ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI