Restriction map for
pF1KB8162
(Circular) (Six-base) MAPSORT of: pF1KB8162.seq Check: 8361 from: 1 to: 4190
pF1KB8162 4190 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1228 1228
Size: 4190
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 944 2015 2447 3194 3462 944
Size: 1071 432 747 268 1672
Fragments arranged by size:
1672 1071 747 432 268
AflIII A'CryG_T
Cuts at: 348 1542 2646 3623 348
Size: 1194 1104 977 915
Fragments arranged by size:
1194 1104 977 915
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AleI CACnn'nnGTG
Cuts at: 880 880
Size: 4190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 544 544
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 1495 3062 1495
Size: 1567 2623
ApaLI G'TGCA_C
Cuts at: 226 2960 226
Size: 2734 1456
ApoI r'AATT_y
Cuts at: 1116 1194 3543 3799 1116
Size: 78 2349 256 1507
Fragments arranged by size:
2349 1507 256 78
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 137 1210 137
Size: 1073 3117
BamHI G'GATC_C
Cuts at: 1215 1215
Size: 4190
BanI G'GyrC_C
Cuts at: 1206 1620 1868 1903 1206
Size: 414 248 35 3493
Fragments arranged by size:
3493 414 248 35
BanII G_rGCy'C
Cuts at: 1021 1170 1204 2234 1021
Size: 149 34 1030 2977
Fragments arranged by size:
2977 1030 149 34
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BbsI GAAGACnn'nnnn_
Cuts at: 242 242
Size: 4190
BbvCI CC'TCA_GC
Cuts at: 251 251
Size: 4190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 142 176 142
Size: 34 4156
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 1710 1710
Size: 4190
BfrBI ATG'CAT
Cuts at: 531 748 531
Size: 217 3973
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 230 617 634 1815 1908 2964 230
Size: 387 17 1181 93 1056 1456
Fragments arranged by size:
1456 1181 1056 387 93 17
BmrI ACTGGGnnnn_n'
Cuts at: 628 1402 1483 1818 3962 628
Size: 774 81 335 2144 856
Fragments arranged by size:
2144 856 774 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 180 2557 180
Size: 2377 1813
Bpu10I CC'TnA_GC
Cuts at: 251 251
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 848 1366 2245 2737 3035 848
Size: 518 879 492 298 2003
Fragments arranged by size:
2003 879 518 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 572 3894 572
Size: 3322 868
BsaAI yAC'GTr
Cuts at: 351 1543 351
Size: 1192 2998
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 1621 1869 2571 4067 1621
Size: 248 702 1496 1744
Fragments arranged by size:
1744 1496 702 248
BsaWI w'CCGG_w
Cuts at: 414 1379 1900 2578 2852 2999 3410 414
Size: 965 521 678 274 147 411 1194
Fragments arranged by size:
1194 965 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 418 418
Size: 4190
BseYI C'CCAG_C
Cuts at: 1615 2373 2950 3446 1615
Size: 758 577 496 2359
Fragments arranged by size:
2359 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 556 256
Size: 300 3890
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 230 759 1204 1982 2172 2964 230
Size: 529 445 778 190 792 1456
Fragments arranged by size:
1456 792 778 529 445 190
BsiWI C'GTAC_G
Cuts at: 643 643
Size: 4190
BsmI GAATG_Cn'
Cuts at: 533 533
Size: 4190
Bsp1286I G_dGCh'C
Cuts at: 230 617 634 759 1021 1170 1204 1815 1908
Size: 387 17 125 262 149 34 611 93
Cuts at: 1908 1982 2172 2234 2964 230
Size: 74 190 62 730 1456
Fragments arranged by size:
1456 730 611 387 262 190 149 125
93 74 62 34 17
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 3366 3366
Size: 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 1240 1454 1756 2137 278
Size: 962 214 302 381 2331
Fragments arranged by size:
2331 962 381 302 214
BsrBI CCG'CTC
Cuts at: 50 465 788 2482 2536 4020 4185 50
Size: 415 323 1694 54 1484 165 55
Fragments arranged by size:
1694 1484 415 323 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2102 2102
Size: 4190
BsrFI r'CCGG_y
Cuts at: 551 2188 2369 2578 551
Size: 1637 181 209 2163
Fragments arranged by size:
2163 1637 209 181
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 475 1567 475
Size: 1092 3098
BstBI TT'CG_AA
Cuts at: 699 2551 699
Size: 1852 2338
BstYI r'GATC_y
Cuts at: 1 1215 1682 1705 2040 2286 3298 3384 3396
Size: 1214 467 23 335 246 1012 86 12
Cuts at: 3396 3742 1
Size: 346 449
Fragments arranged by size:
1214 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 713 107
Size: 606 3584
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2140 2321 2484 2140
Size: 181 163 3846
Fragments arranged by size:
3846 181 163
BtsI GCAGTG_nn'
Cuts at: 485 1021 1433 485
Size: 536 412 3242
Fragments arranged by size:
3242 536 412
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 1775 1949 2340 2367 3712 1775
Size: 174 391 27 1345 2253
Fragments arranged by size:
2253 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 175 2213 2423 175
Size: 2038 210 1942
Fragments arranged by size:
2038 1942 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1202 1202
Size: 4190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 944 2015 2447 3194 3462 944
Size: 1071 432 747 268 1672
Fragments arranged by size:
1672 1071 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 495 1329 495
Size: 834 3356
EcoRI G'AATT_C
Cuts at: 1116 1194 1116
Size: 78 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4190
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 950 1624 1872 2894 3873 950
Size: 674 248 1022 979 1267
Fragments arranged by size:
1267 1022 979 674 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 767 799 2032 2062 2064 2094 767
Size: 32 1233 30 2 30 2863
Fragments arranged by size:
2863 1233 32 30 30 2
HincII GTy'rAC
Cuts at: 1229 1229
Size: 4190
HindIII A'AGCT_T
Cuts at: 283 332 283
Size: 49 4141
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1035 2861 3045 1035
Size: 1826 184 2180
Fragments arranged by size:
2180 1826 184
MscI TGG'CCA
Cuts at: 1951 1951
Size: 4190
MslI CAynn'nnrTG
Cuts at: 304 880 2179 2306 304
Size: 576 1299 127 2188
Fragments arranged by size:
2188 1299 576 127
MspA1I CmG'CkG
Cuts at: 250 808 817 964 1301 1615 1975 2988 3233
Size: 558 9 147 337 314 360 1013 245
Cuts at: 3233 250
Size: 1207
Fragments arranged by size:
1207 1013 558 360 337 314 245 147
9
NaeI GCC'GGC
Cuts at: 553 2371 553
Size: 1818 2372
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NcoI C'CATG_G
Cuts at: 107 713 107
Size: 606 3584
NdeI CA'TA_TG
Cuts at: 750 750
Size: 4190
NgoMIV G'CCGG_C
Cuts at: 551 2369 551
Size: 1818 2372
NsiI A_TGCA'T
Cuts at: 533 750 533
Size: 217 3973
NspI r_CATG'y
Cuts at: 535 1243 1743 2650 535
Size: 708 500 907 2075
Fragments arranged by size:
2075 907 708 500
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PflMI CCAn_nnn'nTGG
Cuts at: 943 943
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PmlI CAC'GTG
Cuts at: 351 351
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PstI C_TGCA'G
Cuts at: 984 1038 1237 1922 984
Size: 54 199 685 3252
Fragments arranged by size:
3252 685 199 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 250 964 1615 1975 250
Size: 714 651 360 2465
Fragments arranged by size:
2465 714 651 360
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 1204 1204
Size: 4190
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SfcI C'TryA_G
Cuts at: 33 146 980 1034 1233 1918 2911 3102 33
Size: 113 834 54 199 685 993 191 1121
Fragments arranged by size:
1121 993 834 685 199 191 113 54
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 139 1212 139
Size: 1073 3117
SmlI C'TyrA_G
Cuts at: 863 1345 2260 2752 3014 863
Size: 482 915 492 262 2039
Fragments arranged by size:
2039 915 492 482 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StyI C'CwwG_G
Cuts at: 107 713 1324 1678 107
Size: 606 611 354 2619
Fragments arranged by size:
2619 611 606 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1178 1773 1906 2365 2574 1178
Size: 595 133 459 209 2794
Fragments arranged by size:
2794 595 459 209 133
TatI w'GTAC_w
Cuts at: 3705 3705
Size: 4190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4158
Tth111I GACn'n_nGTC
Cuts at: 849 1987 849
Size: 1138 3052
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 348 1685 348
Size: 1337 2853
XmaI C'CCGG_G
Cuts at: 137 1210 137
Size: 1073 3117
XmnI GAAnn'nnTTC
Cuts at: 118 118
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BglI BmgBI BmtI BpmI BsaXI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PasI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI