Restriction map for
pF1KB8159
(Circular) (Six-base) MAPSORT of: pF1KB8159.seq Check: 9550 from: 1 to: 3533
pF1KB8159 3533 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 571 571
Size: 3533
Acc65I G'GTAC_C
Cuts at: 549 549
Size: 3533
AclI AA'CG_TT
Cuts at: 3373 3373
Size: 3533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1358 1790 2537 2805 1358
Size: 432 747 268 2086
Fragments arranged by size:
2086 747 432 268
AflIII A'CryG_T
Cuts at: 885 1989 2966 885
Size: 1104 977 1452
Fragments arranged by size:
1452 1104 977
AgeI A'CCGG_T
Cuts at: 1921 1921
Size: 3533
AhdI GACnn_n'nnGTC
Cuts at: 380 380
Size: 3533
AlwNI CAG_nnn'CTG
Cuts at: 838 2405 838
Size: 1567 1966
ApaLI G'TGCA_C
Cuts at: 2303 2303
Size: 3533
ApoI r'AATT_y
Cuts at: 537 2886 3142 537
Size: 2349 256 928
Fragments arranged by size:
2349 928 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3533
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
AvaI C'yCGr_G
Cuts at: 553 553
Size: 3533
BamHI G'GATC_C
Cuts at: 558 558
Size: 3533
BanI G'GyrC_C
Cuts at: 549 963 1211 1246 549
Size: 414 248 35 2836
Fragments arranged by size:
2836 414 248 35
BanII G_rGCy'C
Cuts at: 547 1577 547
Size: 1030 2503
BbeI G_GCGC'C
Cuts at: 967 1215 967
Size: 248 3285
BciVI GTATCCnnnnn_n'
Cuts at: 1431 2192 3533 1431
Size: 761 1341 1431
Fragments arranged by size:
1431 1341 761
BclI T'GATC_A
Cuts at: 326 1053 326
Size: 727 2806
BglII A'GATC_T
Cuts at: 1048 3085 1048
Size: 2037 1496
BlpI GC'TnA_GC
Cuts at: 645 645
Size: 3533
Bme1580I G_kGCm'C
Cuts at: 1158 1251 2307 1158
Size: 93 1056 2384
Fragments arranged by size:
2384 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 745 826 1161 3305 745
Size: 81 335 2144 973
Fragments arranged by size:
2144 973 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1900 1900
Size: 3533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 709 1588 2080 2378 531
Size: 178 879 492 298 1686
Fragments arranged by size:
1686 879 492 298 178
BsaI GGTCTCn'nnnn_
Cuts at: 3237 3237
Size: 3533
BsaAI yAC'GTr
Cuts at: 129 886 129
Size: 757 2776
BsaBI GATnn'nnATC
Cuts at: 6 1047 3084 6
Size: 1041 2037 455
Fragments arranged by size:
2037 1041 455
BsaHI Gr'CG_yC
Cuts at: 188 221 964 1212 1914 3410 188
Size: 33 743 248 702 1496 311
Fragments arranged by size:
1496 743 702 311 248 33
BsaWI w'CCGG_w
Cuts at: 371 722 1243 1921 2195 2342 2753 371
Size: 351 521 678 274 147 411 1151
Fragments arranged by size:
1151 678 521 411 351 274 147
BseYI C'CCAG_C
Cuts at: 958 1716 2293 2789 958
Size: 758 577 496 1702
Fragments arranged by size:
1702 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 342 342
Size: 3533
BsiEI CG_ry'CG
Cuts at: 104 215 1075 1121 2329 104
Size: 111 860 46 1208 1308
Fragments arranged by size:
1308 1208 860 111 46
BsiHKAI G_wGCw'C
Cuts at: 547 1325 1515 2307 547
Size: 778 190 792 1773
Fragments arranged by size:
1773 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 547 1158 1251 1325 1515 1577 2307 547
Size: 611 93 74 190 62 730 1773
Fragments arranged by size:
1773 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 371 2753 371
Size: 2382 1151
BspHI T'CATG_A
Cuts at: 2709 2709
Size: 3533
BspMI ACCTGCnnnn'nnnn_
Cuts at: 583 797 1099 1480 583
Size: 214 302 381 2636
Fragments arranged by size:
2636 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1825 1879 3363 3528 50
Size: 1775 54 1484 165 55
Fragments arranged by size:
1775 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1445 1445
Size: 3533
BsrFI r'CCGG_y
Cuts at: 112 1531 1712 1921 112
Size: 1419 181 209 1724
Fragments arranged by size:
1724 1419 209 181
BssSI C'ACGA_G
Cuts at: 1804 2162 1804
Size: 358 3175
BstAPI GCAn_nnn'nTGC
Cuts at: 910 910
Size: 3533
BstBI TT'CG_AA
Cuts at: 1894 1894
Size: 3533
BstYI r'GATC_y
Cuts at: 1 558 1025 1048 1383 1629 2641 2727 2739
Size: 557 467 23 335 246 1012 86 12
Cuts at: 2739 3085 1
Size: 346 449
Fragments arranged by size:
1012 557 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 209 465 107
Size: 102 256 3175
Fragments arranged by size:
3175 256 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 161 1483 1664 1827 161
Size: 1322 181 163 1867
Fragments arranged by size:
1867 1322 181 163
BtsI GCAGTG_nn'
Cuts at: 776 776
Size: 3533
DraI TTT'AAA
Cuts at: 532 532
Size: 3533
DrdI GACnn_nn'nnGTC
Cuts at: 1239 2097 1239
Size: 858 2675
EaeI y'GGCC_r
Cuts at: 110 212 1118 1292 1683 1710 3055 110
Size: 102 906 174 391 27 1345 588
Fragments arranged by size:
1345 906 588 391 174 102 27
EagI C'GGCC_G
Cuts at: 212 1118 212
Size: 906 2627
EarI CTCTTCn'nnn_
Cuts at: 1556 1766 1556
Size: 210 3323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 488 1741 2051 2197 3343 488
Size: 1253 310 146 1146 678
Fragments arranged by size:
1253 1146 678 310 146
EcoICRI GAG'CTC
Cuts at: 545 545
Size: 3533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1358 1790 2537 2805 1358
Size: 432 747 268 2086
Fragments arranged by size:
2086 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 672 672
Size: 3533
EcoRI G'AATT_C
Cuts at: 537 537
Size: 3533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1552 1552
Size: 3533
FspI TGC'GCA
Cuts at: 1314 1314
Size: 3533
HaeII r_GCGC'y
Cuts at: 967 1215 2237 3216 967
Size: 248 1022 979 1284
Fragments arranged by size:
1284 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 366 398 422 454 1375 1405 1407 1437 366
Size: 32 24 32 921 30 2 30 2462
Fragments arranged by size:
2462 921 32 32 30 30 24 2
HincII GTy'rAC
Cuts at: 572 572
Size: 3533
KasI G'GCGC_C
Cuts at: 963 1211 963
Size: 248 3285
KpnI G_GTAC'C
Cuts at: 553 553
Size: 3533
MluI A'CGCG_T
Cuts at: 2966 2966
Size: 3533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2204 2388 2204
Size: 184 3349
MscI TGG'CCA
Cuts at: 1294 1294
Size: 3533
MslI CAynn'nnrTG
Cuts at: 1522 1649 1522
Size: 127 3406
MspA1I CmG'CkG
Cuts at: 644 958 1318 2331 2576 644
Size: 314 360 1013 245 1601
Fragments arranged by size:
1601 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1714 1714
Size: 3533
NarI GG'CG_CC
Cuts at: 964 1212 964
Size: 248 3285
NcoI C'CATG_G
Cuts at: 107 465 107
Size: 358 3175
NgoMIV G'CCGG_C
Cuts at: 1712 1712
Size: 3533
NspI r_CATG'y
Cuts at: 586 1086 1993 586
Size: 500 907 2126
Fragments arranged by size:
2126 907 500
PciI A'CATG_T
Cuts at: 1989 1989
Size: 3533
PmeI GTTT'AAAC
Cuts at: 532 532
Size: 3533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2672 2704 2672
Size: 32 3501
PstI C_TGCA'G
Cuts at: 580 1265 580
Size: 685 2848
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3533
PvuII CAG'CTG
Cuts at: 958 1318 958
Size: 360 3173
RsrII CG'GwC_CG
Cuts at: 1728 1728
Size: 3533
SacI G_AGCT'C
Cuts at: 547 547
Size: 3533
SalI G'TCGA_C
Cuts at: 570 570
Size: 3533
SapI GCTCTTCn'nnn_
Cuts at: 1556 1766 1556
Size: 210 3323
SbfI CC_TGCA'GG
Cuts at: 580 580
Size: 3533
ScaI AGT'ACT
Cuts at: 3050 3050
Size: 3533
SfcI C'TryA_G
Cuts at: 33 576 1261 2254 2445 33
Size: 543 685 993 191 1121
Fragments arranged by size:
1121 993 685 543 191
SfoI GGC'GCC
Cuts at: 965 1213 965
Size: 248 3285
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
SmaI CCC'GGG
Cuts at: 555 555
Size: 3533
SmlI C'TyrA_G
Cuts at: 510 688 1603 2095 2357 510
Size: 178 915 492 262 1686
Fragments arranged by size:
1686 915 492 262 178
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3533
SphI G_CATG'C
Cuts at: 586 1086 586
Size: 500 3033
StyI C'CwwG_G
Cuts at: 107 465 667 1021 107
Size: 358 202 354 2619
Fragments arranged by size:
2619 358 354 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1116 1249 1708 1917 1116
Size: 133 459 209 2732
Fragments arranged by size:
2732 459 209 133
TatI w'GTAC_w
Cuts at: 3048 3048
Size: 3533
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 3501
Tth111I GACn'n_nGTC
Cuts at: 1330 1330
Size: 3533
XbaI T'CTAG_A
Cuts at: 564 564
Size: 3533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1028 1028
Size: 3533
XmaI C'CCGG_G
Cuts at: 553 553
Size: 3533
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI