Restriction map for
pF1KB8155
(Circular) (Six-base) MAPSORT of: pF1KB8155.seq Check: 2294 from: 1 to: 4493
pF1KB8155 4493 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1131 1131
Size: 4493
AatII G_ACGT'C
Cuts at: 1481 1481
Size: 4493
AccI GT'mk_AC
Cuts at: 1531 1531
Size: 4493
Acc65I G'GTAC_C
Cuts at: 1509 1509
Size: 4493
AclI AA'CG_TT
Cuts at: 4333 4333
Size: 4493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1036 2318 2750 3497 3765 611
Size: 425 1282 432 747 268 1339
Fragments arranged by size:
1339 1282 747 432 425 268
AflIII A'CryG_T
Cuts at: 1845 2949 3926 1845
Size: 1104 977 2412
Fragments arranged by size:
2412 1104 977
AgeI A'CCGG_T
Cuts at: 2881 2881
Size: 4493
AhdI GACnn_n'nnGTC
Cuts at: 879 879
Size: 4493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 923 923
Size: 4493
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 770 802 770
Size: 32 4461
AlwNI CAG_nnn'CTG
Cuts at: 253 691 1798 3365 253
Size: 438 1107 1567 1381
Fragments arranged by size:
1567 1381 1107 438
ApaI G_GGCC'C
Cuts at: 337 337
Size: 4493
ApaLI G'TGCA_C
Cuts at: 1405 3263 1405
Size: 1858 2635
ApoI r'AATT_y
Cuts at: 1497 3846 4102 1497
Size: 2349 256 1888
Fragments arranged by size:
2349 1888 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4493
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4493
AvaI C'yCGr_G
Cuts at: 223 884 1513 223
Size: 661 629 3203
Fragments arranged by size:
3203 661 629
BamHI G'GATC_C
Cuts at: 1518 1518
Size: 4493
BanI G'GyrC_C
Cuts at: 172 1509 1923 2171 2206 172
Size: 1337 414 248 35 2459
Fragments arranged by size:
2459 1337 414 248 35
BanII G_rGCy'C
Cuts at: 337 739 1402 1507 2537 337
Size: 402 663 105 1030 2293
Fragments arranged by size:
2293 1030 663 402 105
BbeI G_GCGC'C
Cuts at: 176 1927 2175 176
Size: 1751 248 2494
Fragments arranged by size:
2494 1751 248
BbsI GAAGACnn'nnnn_
Cuts at: 1413 1413
Size: 4493
BbvCI CC'TCA_GC
Cuts at: 164 164
Size: 4493
BciVI GTATCCnnnnn_n'
Cuts at: 129 2391 3152 4493 129
Size: 2262 761 1341 129
Fragments arranged by size:
2262 1341 761 129
BclI T'GATC_A
Cuts at: 2013 2013
Size: 4493
BglI GCCn_nnn'nGGC
Cuts at: 488 488
Size: 4493
BglII A'GATC_T
Cuts at: 1469 2008 4045 1469
Size: 539 2037 1917
Fragments arranged by size:
2037 1917 539
BlpI GC'TnA_GC
Cuts at: 267 412 684 962 1396 1605 267
Size: 145 272 278 434 209 3155
Fragments arranged by size:
3155 434 278 272 209 145
Bme1580I G_kGCm'C
Cuts at: 337 1409 2118 2211 3267 337
Size: 1072 709 93 1056 1563
Fragments arranged by size:
1563 1072 1056 709 93
BmrI ACTGGGnnnn_n'
Cuts at: 294 873 1705 1786 2121 4265 294
Size: 579 832 81 335 2144 522
Fragments arranged by size:
2144 832 579 522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 282 2860 282
Size: 2578 1915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 1420
Size: 4493
Bpu10I CC'TnA_GC
Cuts at: 164 164
Size: 4493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 1669 2548 3040 3338 758
Size: 911 879 492 298 1913
Fragments arranged by size:
1913 911 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4197 4197
Size: 4493
BsaAI yAC'GTr
Cuts at: 1302 1846 1302
Size: 544 3949
BsaBI GATnn'nnATC
Cuts at: 6 2007 4044 6
Size: 2001 2037 455
Fragments arranged by size:
2037 2001 455
BsaHI Gr'CG_yC
Cuts at: 173 1478 1924 2172 2874 4370 173
Size: 1305 446 248 702 1496 296
Fragments arranged by size:
1496 1305 702 446 296 248
BsaWI w'CCGG_w
Cuts at: 210 231 602 1682 2203 2881 3155 3302 3713
Size: 21 371 1080 521 678 274 147 411
Cuts at: 3713 210
Size: 990
Fragments arranged by size:
1080 990 678 521 411 371 274 147
21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 625 655 811 841 373
Size: 30 222 30 156 30 4025
Fragments arranged by size:
4025 222 156 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 217
Size: 4493
BseYI C'CCAG_C
Cuts at: 336 351 1191 1918 2676 3253 3749 336
Size: 15 840 727 758 577 496 1080
Fragments arranged by size:
1080 840 758 727 577 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1151 1282 1151
Size: 131 4362
BsiEI CG_ry'CG
Cuts at: 104 1247 1251 2035 2081 3289 104
Size: 1143 4 784 46 1208 1308
Fragments arranged by size:
1308 1208 1143 784 46 4
BsiHKAI G_wGCw'C
Cuts at: 413 1402 1409 1507 2285 2475 3267 413
Size: 989 7 98 778 190 792 1639
Fragments arranged by size:
1639 989 792 778 190 98 7
Bsp1286I G_dGCh'C
Cuts at: 337 413 739 1402 1409 1507 2118 2211 2285
Size: 76 326 663 7 98 611 93 74
Cuts at: 2285 2475 2537 3267 337
Size: 190 62 730 1563
Fragments arranged by size:
1563 730 663 611 326 190 98 93
76 74 62 7
BspEI T'CCGG_A
Cuts at: 210 231 602 3713 210
Size: 21 371 3111 990
Fragments arranged by size:
3111 990 371 21
BspHI T'CATG_A
Cuts at: 1370 3669 1370
Size: 2299 2194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 505 1131 1407 1543 1757 2059 2440 505
Size: 626 276 136 214 302 381 2558
Fragments arranged by size:
2558 626 381 302 276 214 136
BsrBI CCG'CTC
Cuts at: 50 2785 2839 4323 4488 50
Size: 2735 54 1484 165 55
Fragments arranged by size:
2735 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2405 2405
Size: 4493
BsrFI r'CCGG_y
Cuts at: 539 1250 2491 2672 2881 539
Size: 711 1241 181 209 2151
Fragments arranged by size:
2151 1241 711 209 181
BssSI C'ACGA_G
Cuts at: 123 358 1069 1483 2764 3122 123
Size: 235 711 414 1281 358 1494
Fragments arranged by size:
1494 1281 711 414 358 235
BstAPI GCAn_nnn'nTGC
Cuts at: 1870 1870
Size: 4493
BstBI TT'CG_AA
Cuts at: 2854 2854
Size: 4493
BstYI r'GATC_y
Cuts at: 1 297 599 715 1469 1518 1985 2008 2343
Size: 296 302 116 754 49 467 23 335
Cuts at: 2343 2589 3601 3687 3699 4045 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 754 467 449 346 335 302 296
246 116 86 49 23 12
BtgI C'CryG_G
Cuts at: 217 456 217
Size: 239 4254
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1209 2443 2624 2787 1209
Size: 1234 181 163 2915
Fragments arranged by size:
2915 1234 181 163
BtsI GCAGTG_nn'
Cuts at: 453 541 583 820 1736 453
Size: 88 42 237 916 3210
Fragments arranged by size:
3210 916 237 88 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 346 381 346
Size: 35 4458
DraI TTT'AAA
Cuts at: 1492 1492
Size: 4493
DrdI GACnn_nn'nnGTC
Cuts at: 241 2199 3057 241
Size: 1958 858 1677
Fragments arranged by size:
1958 1677 858
EaeI y'GGCC_r
Cuts at: 926 1244 2078 2252 2643 2670 4015 926
Size: 318 834 174 391 27 1345 1404
Fragments arranged by size:
1404 1345 834 391 318 174 27
EagI C'GGCC_G
Cuts at: 1244 2078 1244
Size: 834 3659
EarI CTCTTCn'nnn_
Cuts at: 299 2516 2726 299
Size: 2217 210 2066
Fragments arranged by size:
2217 2066 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 506 2701 3011 3157 4303 506
Size: 2195 310 146 1146 696
Fragments arranged by size:
2195 1146 696 310 146
EcoICRI GAG'CTC
Cuts at: 1400 1505 1400
Size: 105 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1036 1420 2318 2750 3497 3765 611
Size: 425 384 898 432 747 268 1339
Fragments arranged by size:
1339 898 747 432 425 384 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1015 1015
Size: 4493
EcoO109I rG'GnC_Cy
Cuts at: 333 1632 333
Size: 1299 3194
EcoRI G'AATT_C
Cuts at: 1497 1497
Size: 4493
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 453 1137 2512 453
Size: 684 1375 2434
Fragments arranged by size:
2434 1375 684
FspI TGC'GCA
Cuts at: 547 1390 2274 547
Size: 843 884 2766
Fragments arranged by size:
2766 884 843
HaeII r_GCGC'y
Cuts at: 176 474 1062 1312 1927 2175 3197 4176 176
Size: 298 588 250 615 248 1022 979 493
Fragments arranged by size:
1022 979 615 588 493 298 250 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 625 657 865 897 2335 2365 2367
Size: 32 220 32 208 32 1438 30 2
Cuts at: 2367 2397 373
Size: 30 2469
Fragments arranged by size:
2469 1438 220 208 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1532 1532
Size: 4493
KasI G'GCGC_C
Cuts at: 172 1923 2171 172
Size: 1751 248 2494
Fragments arranged by size:
2494 1751 248
KpnI G_GTAC'C
Cuts at: 1513 1513
Size: 4493
MluI A'CGCG_T
Cuts at: 3926 3926
Size: 4493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 860 1445 3164 3348 860
Size: 585 1719 184 2005
Fragments arranged by size:
2005 1719 585 184
MscI TGG'CCA
Cuts at: 928 2254 928
Size: 1326 3167
MslI CAynn'nnrTG
Cuts at: 1369 2482 2609 1369
Size: 1113 127 3253
Fragments arranged by size:
3253 1113 127
MspA1I CmG'CkG
Cuts at: 416 769 1350 1604 1918 2278 3291 3536 416
Size: 353 581 254 314 360 1013 245 1373
Fragments arranged by size:
1373 1013 581 360 353 314 254 245
NaeI GCC'GGC
Cuts at: 541 2674 541
Size: 2133 2360
NarI GG'CG_CC
Cuts at: 173 1924 2172 173
Size: 1751 248 2494
Fragments arranged by size:
2494 1751 248
NgoMIV G'CCGG_C
Cuts at: 539 2672 539
Size: 2133 2360
NspI r_CATG'y
Cuts at: 1368 1546 2046 2953 1368
Size: 178 500 907 2908
Fragments arranged by size:
2908 907 500 178
PasI CC'CwG_GG
Cuts at: 864 864
Size: 4493
PciI A'CATG_T
Cuts at: 2949 2949
Size: 4493
PflMI CCAn_nnn'nTGG
Cuts at: 795 795
Size: 4493
PfoI T'CCnGG_A
Cuts at: 941 1436 941
Size: 495 3998
PmeI GTTT'AAAC
Cuts at: 1492 1492
Size: 4493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 4461
PspOMI G'GGCC_C
Cuts at: 333 333
Size: 4493
PstI C_TGCA'G
Cuts at: 277 815 986 1142 1540 2225 277
Size: 538 171 156 398 685 2545
Fragments arranged by size:
2545 685 538 398 171 156
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4493
PvuII CAG'CTG
Cuts at: 416 1350 1918 2278 416
Size: 934 568 360 2631
Fragments arranged by size:
2631 934 568 360
RsrII CG'GwC_CG
Cuts at: 2688 2688
Size: 4493
SacI G_AGCT'C
Cuts at: 1402 1507 1402
Size: 105 4388
SalI G'TCGA_C
Cuts at: 1530 1530
Size: 4493
SapI GCTCTTCn'nnn_
Cuts at: 2516 2726 2516
Size: 210 4283
SbfI CC_TGCA'GG
Cuts at: 1142 1540 1142
Size: 398 4095
ScaI AGT'ACT
Cuts at: 4010 4010
Size: 4493
SfcI C'TryA_G
Cuts at: 33 273 811 982 1107 1138 1536 2221 3214
Size: 240 538 171 125 31 398 685 993
Cuts at: 3214 3405 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 538 398 240 191 171
125 31
SfoI GGC'GCC
Cuts at: 174 1925 2173 174
Size: 1751 248 2494
Fragments arranged by size:
2494 1751 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4493
SmaI CCC'GGG
Cuts at: 225 1515 225
Size: 1290 3203
SmlI C'TyrA_G
Cuts at: 773 1648 2563 3055 3317 773
Size: 875 915 492 262 1949
Fragments arranged by size:
1949 915 875 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4493
SphI G_CATG'C
Cuts at: 1368 1546 2046 1368
Size: 178 500 3815
Fragments arranged by size:
3815 500 178
StuI AGG'CCT
Cuts at: 388 1136 388
Size: 748 3745
StyI C'CwwG_G
Cuts at: 1627 1981 1627
Size: 354 4139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 204 2076 2209 2668 2877 204
Size: 1872 133 459 209 1820
Fragments arranged by size:
1872 1820 459 209 133
TatI w'GTAC_w
Cuts at: 4008 4008
Size: 4493
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4010 4042 4010
Size: 32 4461
Tth111I GACn'n_nGTC
Cuts at: 286 2290 286
Size: 2004 2489
XbaI T'CTAG_A
Cuts at: 1524 1524
Size: 4493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 129 1188 1988 129
Size: 1059 800 2634
Fragments arranged by size:
2634 1059 800
XmaI C'CCGG_G
Cuts at: 223 1513 223
Size: 1290 3203
XmnI GAAnn'nnTTC
Cuts at: 1099 1099
Size: 4493
ZraI GAC'GTC
Cuts at: 1479 1479
Size: 4493
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI AscI AvrII BaeI BcgI BfrBI
BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI