Restriction map for
pF1KB8154
(Circular) (Six-base) MAPSORT of: pF1KB8154.seq Check: 4240 from: 1 to: 4820
pF1KB8154 4820 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1858 1858
Size: 4820
Acc65I G'GTAC_C
Cuts at: 1836 1836
Size: 4820
AclI AA'CG_TT
Cuts at: 850 4660 850
Size: 3810 1010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 921 1005 1257 1341 2645 3077 3824 4092 921
Size: 84 252 84 1304 432 747 268 1649
Fragments arranged by size:
1649 1304 747 432 268 252 84 84
AflIII A'CryG_T
Cuts at: 2172 3276 4253 2172
Size: 1104 977 2739
Fragments arranged by size:
2739 1104 977
AgeI A'CCGG_T
Cuts at: 3208 3208
Size: 4820
AlwNI CAG_nnn'CTG
Cuts at: 2125 3692 2125
Size: 1567 3253
ApaLI G'TGCA_C
Cuts at: 1703 3590 1703
Size: 1887 2933
ApoI r'AATT_y
Cuts at: 147 192 367 803 1139 1223 1559 1643 1727
Size: 45 175 436 336 84 336 84 84
Cuts at: 1727 1824 4173 4429 147
Size: 97 2349 256 538
Fragments arranged by size:
2349 538 436 336 336 256 175 97
84 84 84 45
AseI AT'TA_AT
Cuts at: 20 532 20
Size: 512 4308
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4820
AvaI C'yCGr_G
Cuts at: 1840 1840
Size: 4820
BamHI G'GATC_C
Cuts at: 1845 1845
Size: 4820
BanI G'GyrC_C
Cuts at: 1836 2250 2498 2533 1836
Size: 414 248 35 4123
Fragments arranged by size:
4123 414 248 35
BanII G_rGCy'C
Cuts at: 299 781 1834 2864 299
Size: 482 1053 1030 2255
Fragments arranged by size:
2255 1053 1030 482
BbeI G_GCGC'C
Cuts at: 2254 2502 2254
Size: 248 4572
BbsI GAAGACnn'nnnn_
Cuts at: 473 759 473
Size: 286 4534
BciVI GTATCCnnnnn_n'
Cuts at: 603 1195 2718 3479 4820 603
Size: 592 1523 761 1341 603
Fragments arranged by size:
1523 1341 761 603 592
BclI T'GATC_A
Cuts at: 1377 2340 1377
Size: 963 3857
BfrBI ATG'CAT
Cuts at: 829 829
Size: 4820
BglII A'GATC_T
Cuts at: 2335 4372 2335
Size: 2037 2783
BlpI GC'TnA_GC
Cuts at: 1127 1932 1127
Size: 805 4015
Bme1580I G_kGCm'C
Cuts at: 1707 2445 2538 3594 1707
Size: 738 93 1056 2933
Fragments arranged by size:
2933 1056 738 93
BmrI ACTGGGnnnn_n'
Cuts at: 449 2032 2113 2448 4592 449
Size: 1583 81 335 2144 677
Fragments arranged by size:
2144 1583 677 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3187 3187
Size: 4820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 917 1001 1085 1253 1337 1421 1505 1589
Size: 84 84 84 168 84 84 84 84
Cuts at: 1589 1673 833
Size: 84 3980
Fragments arranged by size:
3980 168 84 84 84 84 84 84
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 1996 2875 3367 3665 140
Size: 1856 879 492 298 1295
Fragments arranged by size:
1856 1295 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1065 1233 4524 1065
Size: 168 3291 1361
Fragments arranged by size:
3291 1361 168
BsaAI yAC'GTr
Cuts at: 2173 2173
Size: 4820
BsaBI GATnn'nnATC
Cuts at: 6 1382 2334 4371 6
Size: 1376 952 2037 455
Fragments arranged by size:
2037 1376 952 455
BsaHI Gr'CG_yC
Cuts at: 2251 2499 3201 4697 2251
Size: 248 702 1496 2374
Fragments arranged by size:
2374 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2009 2530 3208 3482 3629 4040 2009
Size: 521 678 274 147 411 2789
Fragments arranged by size:
2789 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 236 266 236
Size: 30 4790
BseYI C'CCAG_C
Cuts at: 501 2245 3003 3580 4076 501
Size: 1744 758 577 496 1245
Fragments arranged by size:
1744 1245 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2362 2408 3616 104
Size: 2258 46 1208 1308
Fragments arranged by size:
2258 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 781 1707 1834 2612 2802 3594 781
Size: 926 127 778 190 792 2007
Fragments arranged by size:
2007 926 792 778 190 127
BsmI GAATG_Cn'
Cuts at: 951 1539 951
Size: 588 4232
Bsp1286I G_dGCh'C
Cuts at: 299 781 1707 1834 2445 2538 2612 2802 2864
Size: 482 926 127 611 93 74 190 62
Cuts at: 2864 3594 299
Size: 730 1525
Fragments arranged by size:
1525 926 730 611 482 190 127 93
74 62
BspEI T'CCGG_A
Cuts at: 4040 4040
Size: 4820
BspHI T'CATG_A
Cuts at: 1719 3996 1719
Size: 2277 2543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1870 2084 2386 2767 1870
Size: 214 302 381 3923
Fragments arranged by size:
3923 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3112 3166 4650 4815 50
Size: 3062 54 1484 165 55
Fragments arranged by size:
3062 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2732 2732
Size: 4820
BsrFI r'CCGG_y
Cuts at: 2818 2999 3208 2818
Size: 181 209 4430
Fragments arranged by size:
4430 209 181
BsrGI T'GTAC_A
Cuts at: 197 1212 197
Size: 1015 3805
BssSI C'ACGA_G
Cuts at: 3091 3449 3091
Size: 358 4462
BstAPI GCAn_nnn'nTGC
Cuts at: 1291 1543 1711 2197 1291
Size: 252 168 486 3914
Fragments arranged by size:
3914 486 252 168
BstBI TT'CG_AA
Cuts at: 1079 3181 1079
Size: 2102 2718
BstXI CCAn_nnnn'nTGG
Cuts at: 120 120
Size: 4820
BstYI r'GATC_y
Cuts at: 1 1621 1845 2312 2335 2670 2916 3928 4014
Size: 1620 224 467 23 335 246 1012 86
Cuts at: 4014 4026 4372 1
Size: 12 346 449
Fragments arranged by size:
1620 1012 467 449 346 335 246 224
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2770 2951 3114 2770
Size: 181 163 4476
Fragments arranged by size:
4476 181 163
BtsI GCAGTG_nn'
Cuts at: 2063 2063
Size: 4820
DraI TTT'AAA
Cuts at: 357 1819 357
Size: 1462 3358
DrdI GACnn_nn'nnGTC
Cuts at: 2526 3384 2526
Size: 858 3962
EaeI y'GGCC_r
Cuts at: 2405 2579 2970 2997 4342 2405
Size: 174 391 27 1345 2883
Fragments arranged by size:
2883 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2405 2405
Size: 4820
EarI CTCTTCn'nnn_
Cuts at: 797 2843 3053 797
Size: 2046 210 2564
Fragments arranged by size:
2564 2046 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3028 3338 3484 4630 3028
Size: 310 146 1146 3218
Fragments arranged by size:
3218 1146 310 146
EcoICRI GAG'CTC
Cuts at: 779 1832 779
Size: 1053 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 917 921 1001 1005 1085 1253 1257 1337
Size: 84 4 80 4 80 168 4 80
Cuts at: 1337 1341 1421 1505 1589 1673 2645 3077 3824
Size: 4 80 84 84 84 972 432 747
Cuts at: 3824 4092 833
Size: 268 1561
Fragments arranged by size:
1561 972 747 432 268 168 84 84
84 84 80 80 80 80 4 4
4 4
EcoO109I rG'GnC_Cy
Cuts at: 1959 1959
Size: 4820
EcoRI G'AATT_C
Cuts at: 803 1139 1223 1559 1643 1727 1824 803
Size: 336 84 336 84 84 97 3799
Fragments arranged by size:
3799 336 336 97 84 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 571 2839 571
Size: 2268 2552
FspI TGC'GCA
Cuts at: 2601 2601
Size: 4820
HaeII r_GCGC'y
Cuts at: 2254 2502 3524 4503 2254
Size: 248 1022 979 2571
Fragments arranged by size:
2571 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 256 288 1032 1064 2662 2692 2694 2724 256
Size: 32 744 32 1598 30 2 30 2352
Fragments arranged by size:
2352 1598 744 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1859 1859
Size: 4820
KasI G'GCGC_C
Cuts at: 2250 2498 2250
Size: 248 4572
KpnI G_GTAC'C
Cuts at: 1840 1840
Size: 4820
MluI A'CGCG_T
Cuts at: 4253 4253
Size: 4820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 3491 3675 194
Size: 3297 184 1339
Fragments arranged by size:
3297 1339 184
MscI TGG'CCA
Cuts at: 2581 2581
Size: 4820
MslI CAynn'nnrTG
Cuts at: 662 2809 2936 662
Size: 2147 127 2546
Fragments arranged by size:
2546 2147 127
MspA1I CmG'CkG
Cuts at: 501 1931 2245 2605 3618 3863 501
Size: 1430 314 360 1013 245 1458
Fragments arranged by size:
1458 1430 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3001 3001
Size: 4820
NarI GG'CG_CC
Cuts at: 2251 2499 2251
Size: 248 4572
NdeI CA'TA_TG
Cuts at: 390 390
Size: 4820
NgoMIV G'CCGG_C
Cuts at: 2999 2999
Size: 4820
NsiI A_TGCA'T
Cuts at: 831 831
Size: 4820
NspI r_CATG'y
Cuts at: 833 1873 2373 3280 833
Size: 1040 500 907 2373
Fragments arranged by size:
2373 1040 907 500
PciI A'CATG_T
Cuts at: 3276 3276
Size: 4820
PmeI GTTT'AAAC
Cuts at: 1819 1819
Size: 4820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 3959
Size: 32 4788
PstI C_TGCA'G
Cuts at: 1867 2552 1867
Size: 685 4135
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4820
PvuII CAG'CTG
Cuts at: 501 2245 2605 501
Size: 1744 360 2716
Fragments arranged by size:
2716 1744 360
RsrII CG'GwC_CG
Cuts at: 3015 3015
Size: 4820
SacI G_AGCT'C
Cuts at: 781 1834 781
Size: 1053 3767
SalI G'TCGA_C
Cuts at: 1857 1857
Size: 4820
SapI GCTCTTCn'nnn_
Cuts at: 2843 3053 2843
Size: 210 4610
SbfI CC_TGCA'GG
Cuts at: 1867 1867
Size: 4820
ScaI AGT'ACT
Cuts at: 4337 4337
Size: 4820
SfcI C'TryA_G
Cuts at: 33 222 1614 1799 1863 2548 3541 3732 33
Size: 189 1392 185 64 685 993 191 1121
Fragments arranged by size:
1392 1121 993 685 191 189 185 64
SfoI GGC'GCC
Cuts at: 2252 2500 2252
Size: 248 4572
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4820
SmaI CCC'GGG
Cuts at: 1842 1842
Size: 4820
SmlI C'TyrA_G
Cuts at: 155 1975 2890 3382 3644 155
Size: 1820 915 492 262 1331
Fragments arranged by size:
1820 1331 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4820
SphI G_CATG'C
Cuts at: 1873 2373 1873
Size: 500 4320
StuI AGG'CCT
Cuts at: 1102 1438 1541 1102
Size: 336 103 4381
Fragments arranged by size:
4381 336 103
StyI C'CwwG_G
Cuts at: 113 1954 2308 113
Size: 1841 354 2625
Fragments arranged by size:
2625 1841 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2403 2536 2995 3204 2403
Size: 133 459 209 4019
Fragments arranged by size:
4019 459 209 133
TatI w'GTAC_w
Cuts at: 197 1212 4335 197
Size: 1015 3123 682
Fragments arranged by size:
3123 1015 682
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4337 4369 4337
Size: 32 4788
Tth111I GACn'n_nGTC
Cuts at: 2617 2617
Size: 4820
XbaI T'CTAG_A
Cuts at: 1851 1851
Size: 4820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 338 2315 338
Size: 1977 2843
XmaI C'CCGG_G
Cuts at: 1840 1840
Size: 4820
XmnI GAAnn'nnTTC
Cuts at: 554 766 850 934 1270 1354 1690 554
Size: 212 84 84 336 84 336 3684
Fragments arranged by size:
3684 336 336 212 84 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmBI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI