Restriction map for
pF1KB8153
(Circular) (Six-base) MAPSORT of: pF1KB8153.seq Check: 3482 from: 1 to: 4352
pF1KB8153 4352 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1390 1390
Size: 4352
Acc65I G'GTAC_C
Cuts at: 1368 1368
Size: 4352
AclI AA'CG_TT
Cuts at: 4192 4192
Size: 4352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 2177 2609 3356 3624 815
Size: 1362 432 747 268 1543
Fragments arranged by size:
1543 1362 747 432 268
AflIII A'CryG_T
Cuts at: 1704 2808 3785 1704
Size: 1104 977 2271
Fragments arranged by size:
2271 1104 977
AgeI A'CCGG_T
Cuts at: 2740 2740
Size: 4352
AhdI GACnn_n'nnGTC
Cuts at: 210 210
Size: 4352
AleI CACnn'nnGTG
Cuts at: 889 889
Size: 4352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1319 1319
Size: 4352
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 803 835 803
Size: 32 4320
AlwNI CAG_nnn'CTG
Cuts at: 201 264 1657 3224 201
Size: 63 1393 1567 1329
Fragments arranged by size:
1567 1393 1329 63
ApaLI G'TGCA_C
Cuts at: 3122 3122
Size: 4352
ApoI r'AATT_y
Cuts at: 751 1356 3705 3961 751
Size: 605 2349 256 1142
Fragments arranged by size:
2349 1142 605 256
AseI AT'TA_AT
Cuts at: 20 1184 20
Size: 1164 3188
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
AvaI C'yCGr_G
Cuts at: 342 433 669 1232 1372 342
Size: 91 236 563 140 3322
Fragments arranged by size:
3322 563 236 140 91
BamHI G'GATC_C
Cuts at: 1377 1377
Size: 4352
BanI G'GyrC_C
Cuts at: 145 251 405 450 907 1228 1368 1782 2030
Size: 106 154 45 457 321 140 414 248
Cuts at: 2030 2065 145
Size: 35 2432
Fragments arranged by size:
2432 457 414 321 248 154 140 106
45 35
BanII G_rGCy'C
Cuts at: 331 1366 2396 331
Size: 1035 1030 2287
Fragments arranged by size:
2287 1035 1030
BbeI G_GCGC'C
Cuts at: 149 255 409 454 1786 2034 149
Size: 106 154 45 1332 248 2467
Fragments arranged by size:
2467 1332 248 154 106 45
BbsI GAAGACnn'nnnn_
Cuts at: 803 803
Size: 4352
BciVI GTATCCnnnnn_n'
Cuts at: 2250 3011 4352 2250
Size: 761 1341 2250
Fragments arranged by size:
2250 1341 761
BclI T'GATC_A
Cuts at: 1872 1872
Size: 4352
BglI GCCn_nnn'nGGC
Cuts at: 437 437
Size: 4352
BglII A'GATC_T
Cuts at: 1867 3904 1867
Size: 2037 2315
BlpI GC'TnA_GC
Cuts at: 416 536 1464 416
Size: 120 928 3304
Fragments arranged by size:
3304 928 120
Bme1580I G_kGCm'C
Cuts at: 910 1977 2070 3126 910
Size: 1067 93 1056 2136
Fragments arranged by size:
2136 1067 1056 93
BmgBI CAC'GTC
Cuts at: 661 661
Size: 4352
BmrI ACTGGGnnnn_n'
Cuts at: 1564 1645 1980 4124 1564
Size: 81 335 2144 1792
Fragments arranged by size:
2144 1792 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 911 1198 911
Size: 287 4065
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1528 2407 2899 3197 1528
Size: 879 492 298 2683
Fragments arranged by size:
2683 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4056 4056
Size: 4352
BsaAI yAC'GTr
Cuts at: 1705 1705
Size: 4352
BsaBI GATnn'nnATC
Cuts at: 6 1866 3903 6
Size: 1860 2037 455
Fragments arranged by size:
2037 1860 455
BsaHI Gr'CG_yC
Cuts at: 146 252 406 451 1783 2031 2733 4229 146
Size: 106 154 45 1332 248 702 1496 269
Fragments arranged by size:
1496 1332 702 269 248 154 106 45
BsaWI w'CCGG_w
Cuts at: 1040 1145 1541 2062 2740 3014 3161 3572 1040
Size: 105 396 521 678 274 147 411 1820
Fragments arranged by size:
1820 678 521 411 396 274 147 105
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 910 934 1238 910
Size: 24 304 4024
Fragments arranged by size:
4024 304 24
BseYI C'CCAG_C
Cuts at: 330 1172 1777 2535 3112 3608 330
Size: 842 605 758 577 496 1074
Fragments arranged by size:
1074 842 758 605 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 851
Size: 4352
BsiEI CG_ry'CG
Cuts at: 104 302 369 1894 1940 3148 104
Size: 198 67 1525 46 1208 1308
Fragments arranged by size:
1525 1308 1208 198 67 46
BsiHKAI G_wGCw'C
Cuts at: 1366 2144 2334 3126 1366
Size: 778 190 792 2592
Fragments arranged by size:
2592 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 331 910 1366 1977 2070 2144 2334 2396 3126
Size: 579 456 611 93 74 190 62 730
Cuts at: 3126 331
Size: 1557
Fragments arranged by size:
1557 730 611 579 456 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1040 1145 3572 1040
Size: 105 2427 1820
Fragments arranged by size:
2427 1820 105
BspHI T'CATG_A
Cuts at: 3528 3528
Size: 4352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1402 1616 1918 2299 1402
Size: 214 302 381 3455
Fragments arranged by size:
3455 381 302 214
BsrBI CCG'CTC
Cuts at: 50 540 2644 2698 4182 4347 50
Size: 490 2104 54 1484 165 55
Fragments arranged by size:
2104 1484 490 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2264 2264
Size: 4352
BsrFI r'CCGG_y
Cuts at: 248 307 355 2350 2531 2740 248
Size: 59 48 1995 181 209 1860
Fragments arranged by size:
1995 1860 209 181 59 48
BssSI C'ACGA_G
Cuts at: 2623 2981 2623
Size: 358 3994
BstAPI GCAn_nnn'nTGC
Cuts at: 264 1729 264
Size: 1465 2887
BstBI TT'CG_AA
Cuts at: 822 2713 822
Size: 1891 2461
BstEII G'GTnAC_C
Cuts at: 1192 1192
Size: 4352
BstXI CCAn_nnnn'nTGG
Cuts at: 855 891 1074 855
Size: 36 183 4133
Fragments arranged by size:
4133 183 36
BstYI r'GATC_y
Cuts at: 1 544 1377 1844 1867 2202 2448 3460 3546
Size: 543 833 467 23 335 246 1012 86
Cuts at: 3546 3558 3904 1
Size: 12 346 449
Fragments arranged by size:
1012 833 543 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 131 315 507 1073 131
Size: 184 192 566 3410
Fragments arranged by size:
3410 566 192 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 166 2302 2483 2646 166
Size: 2136 181 163 1872
Fragments arranged by size:
2136 1872 181 163
BtsI GCAGTG_nn'
Cuts at: 1595 1595
Size: 4352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 789 824 789
Size: 35 4317
DraI TTT'AAA
Cuts at: 1351 1351
Size: 4352
DrdI GACnn_nn'nnGTC
Cuts at: 2058 2916 2058
Size: 858 3494
EaeI y'GGCC_r
Cuts at: 179 299 366 1937 2111 2502 2529 3874 179
Size: 120 67 1571 174 391 27 1345 657
Fragments arranged by size:
1571 1345 657 391 174 120 67 27
EagI C'GGCC_G
Cuts at: 299 366 1937 299
Size: 67 1571 2714
Fragments arranged by size:
2714 1571 67
EarI CTCTTCn'nnn_
Cuts at: 471 911 2375 2585 471
Size: 440 1464 210 2238
Fragments arranged by size:
2238 1464 440 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2560 2870 3016 4162 2560
Size: 310 146 1146 2750
Fragments arranged by size:
2750 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1364 1364
Size: 4352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 911 1198 2177 2609 3356 3624 815
Size: 96 287 979 432 747 268 1543
Fragments arranged by size:
1543 979 747 432 287 268 96
EcoNI CCTnn'n_nnAGG
Cuts at: 235 476 235
Size: 241 4111
EcoO109I rG'GnC_Cy
Cuts at: 338 1491 338
Size: 1153 3199
EcoRI G'AATT_C
Cuts at: 1356 1356
Size: 4352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4352
FspI TGC'GCA
Cuts at: 2133 2133
Size: 4352
HaeII r_GCGC'y
Cuts at: 119 149 249 255 409 454 1786 2034 3056
Size: 30 100 6 154 45 1332 248 1022
Cuts at: 3056 4035 119
Size: 979 436
Fragments arranged by size:
1332 1022 979 436 248 154 100 45
30 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 529 561 1153 1185 2194 2224 2226
Size: 32 301 32 592 32 1009 30 2
Cuts at: 2226 2256 196
Size: 30 2292
Fragments arranged by size:
2292 1009 592 301 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1391 1391
Size: 4352
KasI G'GCGC_C
Cuts at: 145 251 405 450 1782 2030 145
Size: 106 154 45 1332 248 2467
Fragments arranged by size:
2467 1332 248 154 106 45
KpnI G_GTAC'C
Cuts at: 1372 1372
Size: 4352
MluI A'CGCG_T
Cuts at: 3785 3785
Size: 4352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 926 3023 3207 194
Size: 732 2097 184 1339
Fragments arranged by size:
2097 1339 732 184
MscI TGG'CCA
Cuts at: 181 2113 181
Size: 1932 2420
MslI CAynn'nnrTG
Cuts at: 889 2341 2468 889
Size: 1452 127 2773
Fragments arranged by size:
2773 1452 127
MspA1I CmG'CkG
Cuts at: 142 198 264 317 420 598 1176 1463 1777
Size: 56 66 53 103 178 578 287 314
Cuts at: 1777 2137 3150 3395 142
Size: 360 1013 245 1099
Fragments arranged by size:
1099 1013 578 360 314 287 245 178
103 66 56 53
NaeI GCC'GGC
Cuts at: 250 357 2533 250
Size: 107 2176 2069
Fragments arranged by size:
2176 2069 107
NarI GG'CG_CC
Cuts at: 146 252 406 451 1783 2031 146
Size: 106 154 45 1332 248 2467
Fragments arranged by size:
2467 1332 248 154 106 45
NgoMIV G'CCGG_C
Cuts at: 248 355 2531 248
Size: 107 2176 2069
Fragments arranged by size:
2176 2069 107
NspI r_CATG'y
Cuts at: 1405 1905 2812 1405
Size: 500 907 2945
Fragments arranged by size:
2945 907 500
PasI CC'CwG_GG
Cuts at: 706 903 706
Size: 197 4155
PciI A'CATG_T
Cuts at: 2808 2808
Size: 4352
PmeI GTTT'AAAC
Cuts at: 1351 1351
Size: 4352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 4320
PstI C_TGCA'G
Cuts at: 269 449 1399 2084 269
Size: 180 950 685 2537
Fragments arranged by size:
2537 950 685 180
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4352
PvuII CAG'CTG
Cuts at: 198 264 598 1176 1777 2137 198
Size: 66 334 578 601 360 2413
Fragments arranged by size:
2413 601 578 360 334 66
RsrII CG'GwC_CG
Cuts at: 2547 2547
Size: 4352
SacI G_AGCT'C
Cuts at: 1366 1366
Size: 4352
SacII CC_GC'GG
Cuts at: 318 318
Size: 4352
SalI G'TCGA_C
Cuts at: 1389 1389
Size: 4352
SapI GCTCTTCn'nnn_
Cuts at: 2375 2585 2375
Size: 210 4142
SbfI CC_TGCA'GG
Cuts at: 449 1399 449
Size: 950 3402
ScaI AGT'ACT
Cuts at: 3869 3869
Size: 4352
SexAI A'CCwGG_T
Cuts at: 621 621
Size: 4352
SfcI C'TryA_G
Cuts at: 33 265 445 549 1395 2080 3073 3264 33
Size: 232 180 104 846 685 993 191 1121
Fragments arranged by size:
1121 993 846 685 232 191 180 104
SfoI GGC'GCC
Cuts at: 147 253 407 452 1784 2032 147
Size: 106 154 45 1332 248 2467
Fragments arranged by size:
2467 1332 248 154 106 45
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
SgrAI Cr'CCGG_yG
Cuts at: 248 248
Size: 4352
SmaI CCC'GGG
Cuts at: 435 1234 1374 435
Size: 799 140 3413
Fragments arranged by size:
3413 799 140
SmlI C'TyrA_G
Cuts at: 1507 2422 2914 3176 1507
Size: 915 492 262 2683
Fragments arranged by size:
2683 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4352
SphI G_CATG'C
Cuts at: 1405 1905 1405
Size: 500 3852
StyI C'CwwG_G
Cuts at: 1486 1840 1486
Size: 354 3998
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 796 1935 2068 2527 2736 796
Size: 1139 133 459 209 2412
Fragments arranged by size:
2412 1139 459 209 133
TatI w'GTAC_w
Cuts at: 3867 3867
Size: 4352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 4320
Tth111I GACn'n_nGTC
Cuts at: 2149 2149
Size: 4352
XbaI T'CTAG_A
Cuts at: 1383 1383
Size: 4352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1284 1847 1284
Size: 563 3789
XmaI C'CCGG_G
Cuts at: 433 1232 1372 433
Size: 799 140 3413
Fragments arranged by size:
3413 799 140
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI