Restriction map for
pF1KB8150
(Circular) (Six-base) MAPSORT of: pF1KB8150.seq Check: 3540 from: 1 to: 4550
pF1KB8150 4550 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1212 1588 1212
Size: 376 4174
Acc65I G'GTAC_C
Cuts at: 1566 1566
Size: 4550
AclI AA'CG_TT
Cuts at: 4390 4390
Size: 4550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2375 2807 3554 3822 2375
Size: 432 747 268 3103
Fragments arranged by size:
3103 747 432 268
AflIII A'CryG_T
Cuts at: 678 1902 3006 3983 678
Size: 1224 1104 977 1245
Fragments arranged by size:
1245 1224 1104 977
AgeI A'CCGG_T
Cuts at: 2938 2938
Size: 4550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 333 333
Size: 4550
AlwNI CAG_nnn'CTG
Cuts at: 385 778 1855 3422 385
Size: 393 1077 1567 1513
Fragments arranged by size:
1567 1513 1077 393
ApaI G_GGCC'C
Cuts at: 751 751
Size: 4550
ApaLI G'TGCA_C
Cuts at: 3320 3320
Size: 4550
ApoI r'AATT_y
Cuts at: 791 814 1152 1554 3903 4159 791
Size: 23 338 402 2349 256 1182
Fragments arranged by size:
2349 1182 402 338 256 23
AseI AT'TA_AT
Cuts at: 20 1310 20
Size: 1290 3260
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
AvaI C'yCGr_G
Cuts at: 302 1570 302
Size: 1268 3282
BamHI G'GATC_C
Cuts at: 298 1575 298
Size: 1277 3273
BanI G'GyrC_C
Cuts at: 1566 1980 2228 2263 1566
Size: 414 248 35 3853
Fragments arranged by size:
3853 414 248 35
BanII G_rGCy'C
Cuts at: 751 869 1564 2594 751
Size: 118 695 1030 2707
Fragments arranged by size:
2707 1030 695 118
BbeI G_GCGC'C
Cuts at: 1984 2232 1984
Size: 248 4302
BbsI GAAGACnn'nnnn_
Cuts at: 266 362 266
Size: 96 4454
BciVI GTATCCnnnnn_n'
Cuts at: 2448 3209 4550 2448
Size: 761 1341 2448
Fragments arranged by size:
2448 1341 761
BclI T'GATC_A
Cuts at: 2070 2070
Size: 4550
BglII A'GATC_T
Cuts at: 2065 4102 2065
Size: 2037 2513
BlpI GC'TnA_GC
Cuts at: 1662 1662
Size: 4550
Bme1580I G_kGCm'C
Cuts at: 751 2175 2268 3324 751
Size: 1424 93 1056 1977
Fragments arranged by size:
1977 1424 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1438 1762 1843 2178 4322 1438
Size: 324 81 335 2144 1666
Fragments arranged by size:
2144 1666 335 324 81
BmtI G_CTAG'C
Cuts at: 1369 1369
Size: 4550
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 1360 755
Size: 605 3945
Bpu10I CC'TnA_GC
Cuts at: 184 530 184
Size: 346 4204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 913 1726 2605 3097 3395 412
Size: 501 813 879 492 298 1567
Fragments arranged by size:
1567 879 813 501 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1014 4254 1014
Size: 3240 1310
BsaAI yAC'GTr
Cuts at: 565 1903 565
Size: 1338 3212
BsaBI GATnn'nnATC
Cuts at: 6 2064 4101 6
Size: 2058 2037 455
Fragments arranged by size:
2058 2037 455
BsaHI Gr'CG_yC
Cuts at: 1981 2229 2931 4427 1981
Size: 248 702 1496 2104
Fragments arranged by size:
2104 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1739 2260 2938 3212 3359 3770 1739
Size: 521 678 274 147 411 2519
Fragments arranged by size:
2519 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 650 680 650
Size: 30 4520
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 877 1357 877
Size: 480 4070
BseYI C'CCAG_C
Cuts at: 160 1975 2733 3310 3806 160
Size: 1815 758 577 496 904
Fragments arranged by size:
1815 904 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2092 2138 3346 104
Size: 1988 46 1208 1308
Fragments arranged by size:
1988 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 711 869 1564 2342 2532 3324 711
Size: 158 695 778 190 792 1937
Fragments arranged by size:
1937 792 778 695 190 158
BsmI GAATG_Cn'
Cuts at: 1106 1106
Size: 4550
Bsp1286I G_dGCh'C
Cuts at: 711 751 869 1564 2175 2268 2342 2532 2594
Size: 40 118 695 611 93 74 190 62
Cuts at: 2594 3324 711
Size: 730 1937
Fragments arranged by size:
1937 730 695 611 190 118 93 74
62 40
BspEI T'CCGG_A
Cuts at: 3770 3770
Size: 4550
BspHI T'CATG_A
Cuts at: 3726 3726
Size: 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1600 1814 2116 2497 1600
Size: 214 302 381 3653
Fragments arranged by size:
3653 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2842 2896 4380 4545 50
Size: 2792 54 1484 165 55
Fragments arranged by size:
2792 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2462 2462
Size: 4550
BsrFI r'CCGG_y
Cuts at: 2548 2729 2938 2548
Size: 181 209 4160
Fragments arranged by size:
4160 209 181
BssSI C'ACGA_G
Cuts at: 704 2821 3179 704
Size: 2117 358 2075
Fragments arranged by size:
2117 2075 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1927 1927
Size: 4550
BstBI TT'CG_AA
Cuts at: 1423 2911 1423
Size: 1488 3062
BstYI r'GATC_y
Cuts at: 1 298 382 839 1575 2042 2065 2400 2646
Size: 297 84 457 736 467 23 335 246
Cuts at: 2646 3658 3744 3756 4102 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 736 467 457 449 346 335 297
246 86 84 23 12
Bsu36I CC'TnA_GG
Cuts at: 779 779
Size: 4550
BtgI C'CryG_G
Cuts at: 107 107
Size: 4550
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2500 2681 2844 2500
Size: 181 163 4206
Fragments arranged by size:
4206 181 163
BtsI GCAGTG_nn'
Cuts at: 515 1793 515
Size: 1278 3272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 491 526 491
Size: 35 4515
DraI TTT'AAA
Cuts at: 1549 1549
Size: 4550
DrdI GACnn_nn'nnGTC
Cuts at: 2256 3114 2256
Size: 858 3692
EaeI y'GGCC_r
Cuts at: 2135 2309 2700 2727 4072 2135
Size: 174 391 27 1345 2613
Fragments arranged by size:
2613 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2135 2135
Size: 4550
EarI CTCTTCn'nnn_
Cuts at: 800 1064 2573 2783 800
Size: 264 1509 210 2567
Fragments arranged by size:
2567 1509 264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2758 3068 3214 4360 2758
Size: 310 146 1146 2948
Fragments arranged by size:
2948 1146 310 146
EcoICRI GAG'CTC
Cuts at: 867 1562 867
Size: 695 3855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 1360 2375 2807 3554 3822 755
Size: 605 1015 432 747 268 1483
Fragments arranged by size:
1483 1015 747 605 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1166 1166
Size: 4550
EcoO109I rG'GnC_Cy
Cuts at: 747 1241 1689 747
Size: 494 448 3608
Fragments arranged by size:
3608 494 448
EcoRI G'AATT_C
Cuts at: 1554 1554
Size: 4550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 4550
FspI TGC'GCA
Cuts at: 1126 2331 1126
Size: 1205 3345
FspAI rTGC'GCAy
Cuts at: 1126 1126
Size: 4550
HaeII r_GCGC'y
Cuts at: 1984 2232 3254 4233 1984
Size: 248 1022 979 2301
Fragments arranged by size:
2301 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1228 1260 1296 1328 2392 2422 2424 2454 1228
Size: 32 36 32 1064 30 2 30 3324
Fragments arranged by size:
3324 1064 36 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1589 1589
Size: 4550
KasI G'GCGC_C
Cuts at: 1980 2228 1980
Size: 248 4302
KpnI G_GTAC'C
Cuts at: 1570 1570
Size: 4550
MfeI C'AATT_G
Cuts at: 924 924
Size: 4550
MluI A'CGCG_T
Cuts at: 3983 3983
Size: 4550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1022 3221 3405 1022
Size: 2199 184 2167
Fragments arranged by size:
2199 2167 184
MscI TGG'CCA
Cuts at: 2311 2311
Size: 4550
MslI CAynn'nnrTG
Cuts at: 2539 2666 2539
Size: 127 4423
MspA1I CmG'CkG
Cuts at: 232 370 1405 1661 1975 2335 3348 3593 232
Size: 138 1035 256 314 360 1013 245 1189
Fragments arranged by size:
1189 1035 1013 360 314 256 245 138
NaeI GCC'GGC
Cuts at: 2731 2731
Size: 4550
NarI GG'CG_CC
Cuts at: 1981 2229 1981
Size: 248 4302
NcoI C'CATG_G
Cuts at: 107 107
Size: 4550
NgoMIV G'CCGG_C
Cuts at: 2729 2729
Size: 4550
NheI G'CTAG_C
Cuts at: 1365 1365
Size: 4550
NspI r_CATG'y
Cuts at: 195 459 1603 2103 3010 195
Size: 264 1144 500 907 1735
Fragments arranged by size:
1735 1144 907 500 264
PciI A'CATG_T
Cuts at: 3006 3006
Size: 4550
PmeI GTTT'AAAC
Cuts at: 1549 1549
Size: 4550
PmlI CAC'GTG
Cuts at: 565 565
Size: 4550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 4518
PspOMI G'GGCC_C
Cuts at: 747 747
Size: 4550
PstI C_TGCA'G
Cuts at: 510 1140 1597 2282 510
Size: 630 457 685 2778
Fragments arranged by size:
2778 685 630 457
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4550
PvuII CAG'CTG
Cuts at: 232 370 1405 1975 2335 232
Size: 138 1035 570 360 2447
Fragments arranged by size:
2447 1035 570 360 138
RsrII CG'GwC_CG
Cuts at: 2745 2745
Size: 4550
SacI G_AGCT'C
Cuts at: 869 1564 869
Size: 695 3855
SalI G'TCGA_C
Cuts at: 1587 1587
Size: 4550
SapI GCTCTTCn'nnn_
Cuts at: 2573 2783 2573
Size: 210 4340
SbfI CC_TGCA'GG
Cuts at: 1597 1597
Size: 4550
ScaI AGT'ACT
Cuts at: 4067 4067
Size: 4550
SfcI C'TryA_G
Cuts at: 33 506 1136 1593 2278 3271 3462 33
Size: 473 630 457 685 993 191 1121
Fragments arranged by size:
1121 993 685 630 473 457 191
SfoI GGC'GCC
Cuts at: 1982 2230 1982
Size: 248 4302
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
SmaI CCC'GGG
Cuts at: 1572 1572
Size: 4550
SmlI C'TyrA_G
Cuts at: 391 892 1705 2620 3112 3374 391
Size: 501 813 915 492 262 1567
Fragments arranged by size:
1567 915 813 501 492 262
SpeI A'CTAG_T
Cuts at: 67 981 1354 67
Size: 914 373 3263
Fragments arranged by size:
3263 914 373
SphI G_CATG'C
Cuts at: 195 1603 2103 195
Size: 1408 500 2642
Fragments arranged by size:
2642 1408 500
StyI C'CwwG_G
Cuts at: 107 628 1004 1684 2038 107
Size: 521 376 680 354 2619
Fragments arranged by size:
2619 680 521 376 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 2133 2266 2725 2934 100
Size: 2033 133 459 209 1716
Fragments arranged by size:
2033 1716 459 209 133
TatI w'GTAC_w
Cuts at: 978 4065 978
Size: 3087 1463
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 191 223 290 322 650 682 904 936 1268
Size: 32 67 32 328 32 222 32 332
Cuts at: 1268 1300 1430 1462 4067 4099 191
Size: 32 130 32 2605 32 642
Fragments arranged by size:
2605 642 332 328 222 130 67 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2347 2347
Size: 4550
XbaI T'CTAG_A
Cuts at: 1581 1581
Size: 4550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2045 2045
Size: 4550
XmaI C'CCGG_G
Cuts at: 1570 1570
Size: 4550
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PmeI PmlI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
DraIII EcoRV FseI HindIII HpaI NdeI NotI NruI
NsiI PacI PasI PflMI PfoI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI