Restriction map for
pF1KB8148
(Circular) (Six-base) MAPSORT of: pF1KB8148.seq Check: 9869 from: 1 to: 4742
pF1KB8148 4742 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1780 1780
Size: 4742
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 796 1544 1604 1634 1697 2567 2999 3746
Size: 694 748 60 30 63 870 432 747
Cuts at: 3746 4014 102
Size: 268 830
Fragments arranged by size:
870 830 748 747 694 432 268 63
60 30
AfeI AGC'GCT
Cuts at: 1091 1143 1091
Size: 52 4690
AflIII A'CryG_T
Cuts at: 2094 3198 4175 2094
Size: 1104 977 2661
Fragments arranged by size:
2661 1104 977
AgeI A'CCGG_T
Cuts at: 3130 3130
Size: 4742
AhdI GACnn_n'nnGTC
Cuts at: 357 1020 357
Size: 663 4079
AleI CACnn'nnGTG
Cuts at: 1318 1318
Size: 4742
AlwNI CAG_nnn'CTG
Cuts at: 1113 2047 3614 1113
Size: 934 1567 2241
Fragments arranged by size:
2241 1567 934
ApaLI G'TGCA_C
Cuts at: 178 430 784 3512 178
Size: 252 354 2728 1408
Fragments arranged by size:
2728 1408 354 252
ApoI r'AATT_y
Cuts at: 1746 4095 4351 1746
Size: 2349 256 2137
Fragments arranged by size:
2349 2137 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 173 733 1762 173
Size: 560 1029 3153
Fragments arranged by size:
3153 1029 560
BamHI G'GATC_C
Cuts at: 1767 1767
Size: 4742
BanI G'GyrC_C
Cuts at: 240 700 1758 2172 2420 2455 240
Size: 460 1058 414 248 35 2527
Fragments arranged by size:
2527 1058 460 414 248 35
BanII G_rGCy'C
Cuts at: 734 1354 1517 1756 2786 734
Size: 620 163 239 1030 2690
Fragments arranged by size:
2690 1030 620 239 163
BbeI G_GCGC'C
Cuts at: 244 704 2176 2424 244
Size: 460 1472 248 2562
Fragments arranged by size:
2562 1472 460 248
BbsI GAAGACnn'nnnn_
Cuts at: 242 242
Size: 4742
BbvCI CC'TCA_GC
Cuts at: 935 1496 935
Size: 561 4181
BciVI GTATCCnnnnn_n'
Cuts at: 2640 3401 4742 2640
Size: 761 1341 2640
Fragments arranged by size:
2640 1341 761
BclI T'GATC_A
Cuts at: 2262 2262
Size: 4742
BglI GCCn_nnn'nGGC
Cuts at: 709 709
Size: 4742
BglII A'GATC_T
Cuts at: 833 2257 4294 833
Size: 1424 2037 1281
Fragments arranged by size:
2037 1424 1281
BlpI GC'TnA_GC
Cuts at: 219 655 1854 219
Size: 436 1199 3107
Fragments arranged by size:
3107 1199 436
Bme1580I G_kGCm'C
Cuts at: 182 434 788 2367 2460 3516 182
Size: 252 354 1579 93 1056 1408
Fragments arranged by size:
1579 1408 1056 354 252 93
BmgBI CAC'GTC
Cuts at: 852 852
Size: 4742
BmrI ACTGGGnnnn_n'
Cuts at: 299 1954 2035 2370 4514 299
Size: 1655 81 335 2144 527
Fragments arranged by size:
2144 1655 527 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1536 3109 1536
Size: 1573 3169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 213 267 331 373 460 571 628 1114
Size: 42 54 64 42 87 111 57 486
Cuts at: 1114 1135 171
Size: 21 3778
Fragments arranged by size:
3778 486 111 87 64 57 54 42
42 21
Bpu10I CC'TnA_GC
Cuts at: 325 935 1496 325
Size: 610 561 3571
Fragments arranged by size:
3571 610 561
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 865 1027 1918 2797 3289 3587 340
Size: 525 162 891 879 492 298 1495
Fragments arranged by size:
1495 891 879 525 492 298 162
BsaI GGTCTCn'nnnn_
Cuts at: 607 1291 4446 607
Size: 684 3155 903
Fragments arranged by size:
3155 903 684
BsaAI yAC'GTr
Cuts at: 162 2095 162
Size: 1933 2809
BsaBI GATnn'nnATC
Cuts at: 6 2256 4293 6
Size: 2250 2037 455
Fragments arranged by size:
2250 2037 455
BsaHI Gr'CG_yC
Cuts at: 169 241 668 701 995 2173 2421 3123 4619
Size: 72 427 33 294 1178 248 702 1496
Cuts at: 4619 169
Size: 292
Fragments arranged by size:
1496 1178 702 427 294 292 248 72
33
BsaWI w'CCGG_w
Cuts at: 1931 2452 3130 3404 3551 3962 1931
Size: 521 678 274 147 411 2711
Fragments arranged by size:
2711 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 127
Size: 30 4712
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 270 625 661 808 1222 1309 1468 1552 1555
Size: 355 36 147 414 87 159 84 3
Cuts at: 1555 1558 1588 1696 1699 270
Size: 3 30 108 3 3313
Fragments arranged by size:
3313 414 355 159 147 108 87 84
36 30 3 3 3
BseYI C'CCAG_C
Cuts at: 1705 2167 2925 3502 3998 1705
Size: 462 758 577 496 2449
Fragments arranged by size:
2449 758 577 496 462
BsiEI CG_ry'CG
Cuts at: 104 2284 2330 3538 104
Size: 2180 46 1208 1308
Fragments arranged by size:
2180 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 182 434 734 788 1354 1756 2534 2724 3516
Size: 252 300 54 566 402 778 190 792
Cuts at: 3516 182
Size: 1408
Fragments arranged by size:
1408 792 778 566 402 300 252 190
54
BsmBI CGTCTCn'nnnn_
Cuts at: 160 160
Size: 4742
Bsp1286I G_dGCh'C
Cuts at: 182 434 734 788 1354 1517 1756 2367 2460
Size: 252 300 54 566 163 239 611 93
Cuts at: 2460 2534 2724 2786 3516 182
Size: 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 566 300 252 239 190
163 93 74 62 54
BspEI T'CCGG_A
Cuts at: 3962 3962
Size: 4742
BspHI T'CATG_A
Cuts at: 3918 3918
Size: 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 551 635 1792 2006 2308 2689 551
Size: 84 1157 214 302 381 2604
Fragments arranged by size:
2604 1157 381 302 214 84
BsrBI CCG'CTC
Cuts at: 50 1418 3034 3088 4572 4737 50
Size: 1368 1616 54 1484 165 55
Fragments arranged by size:
1616 1484 1368 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2654 2654
Size: 4742
BsrFI r'CCGG_y
Cuts at: 243 677 2740 2921 3130 243
Size: 434 2063 181 209 1855
Fragments arranged by size:
2063 1855 434 209 181
BssHII G'CGCG_C
Cuts at: 634 691 893 1002 634
Size: 57 202 109 4374
Fragments arranged by size:
4374 202 109 57
BssSI C'ACGA_G
Cuts at: 433 3013 3371 433
Size: 2580 358 1804
Fragments arranged by size:
2580 1804 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2119 2119
Size: 4742
BstBI TT'CG_AA
Cuts at: 3103 3103
Size: 4742
BstXI CCAn_nnnn'nTGG
Cuts at: 1056 1363 1056
Size: 307 4435
BstYI r'GATC_y
Cuts at: 1 533 818 833 1767 2234 2257 2592 2838
Size: 532 285 15 934 467 23 335 246
Cuts at: 2838 3850 3936 3948 4294 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 934 532 467 449 346 335 285
246 86 23 15 12
BtgI C'CryG_G
Cuts at: 839 839
Size: 4742
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2692 2873 3036 2692
Size: 181 163 4398
Fragments arranged by size:
4398 181 163
BtsI GCAGTG_nn'
Cuts at: 1985 1985
Size: 4742
DraI TTT'AAA
Cuts at: 1741 1741
Size: 4742
DrdI GACnn_nn'nnGTC
Cuts at: 2448 3306 2448
Size: 858 3884
EaeI y'GGCC_r
Cuts at: 911 1365 2327 2501 2892 2919 4264 911
Size: 454 962 174 391 27 1345 1389
Fragments arranged by size:
1389 1345 962 454 391 174 27
EagI C'GGCC_G
Cuts at: 2327 2327
Size: 4742
EarI CTCTTCn'nnn_
Cuts at: 638 785 1553 1565 1580 1601 1673 2765 2975
Size: 147 768 12 15 21 72 1092 210
Cuts at: 2975 638
Size: 2405
Fragments arranged by size:
2405 1092 768 210 147 72 21 15
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 571 861 2950 3260 3406 4552 571
Size: 290 2089 310 146 1146 761
Fragments arranged by size:
2089 1146 761 310 290 146
EcoICRI GAG'CTC
Cuts at: 732 1352 1754 732
Size: 620 402 3720
Fragments arranged by size:
3720 620 402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 171 213 267 331 373 460 571 628
Size: 69 42 54 64 42 87 111 57
Cuts at: 628 796 1114 1135 1544 1604 1634 1697 2567
Size: 168 318 21 409 60 30 63 870
Cuts at: 2567 2999 3746 4014 102
Size: 432 747 268 830
Fragments arranged by size:
870 830 747 432 409 318 268 168
111 87 69 64 63 60 57 54
42 42 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 328 328
Size: 4742
EcoO109I rG'GnC_Cy
Cuts at: 393 456 1881 393
Size: 63 1425 3254
Fragments arranged by size:
3254 1425 63
EcoRI G'AATT_C
Cuts at: 1746 1746
Size: 4742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 972 2761 972
Size: 1789 2953
FspI TGC'GCA
Cuts at: 196 622 2523 196
Size: 426 1901 2415
Fragments arranged by size:
2415 1901 426
HaeII r_GCGC'y
Cuts at: 159 244 520 586 704 718 980 1093 1145
Size: 85 276 66 118 14 262 113 52
Cuts at: 1145 2176 2424 3446 4425 159
Size: 1031 248 1022 979 476
Fragments arranged by size:
1031 1022 979 476 276 262 248 118
113 85 66 52 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 291 323 343 375 751 783 1006 1020 1038
Size: 32 20 32 376 32 223 14 18
Cuts at: 1038 1052 2584 2614 2616 2646 291
Size: 14 1532 30 2 30 2387
Fragments arranged by size:
2387 1532 376 223 32 32 32 30
30 20 18 14 14 2
HincII GTy'rAC
Cuts at: 1781 1781
Size: 4742
KasI G'GCGC_C
Cuts at: 240 700 2172 2420 240
Size: 460 1472 248 2562
Fragments arranged by size:
2562 1472 460 248
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3413 3597 3413
Size: 184 4558
MscI TGG'CCA
Cuts at: 2503 2503
Size: 4742
MslI CAynn'nnrTG
Cuts at: 1318 2731 2858 1318
Size: 1413 127 3202
Fragments arranged by size:
3202 1413 127
MspA1I CmG'CkG
Cuts at: 200 1104 1582 1853 2167 2527 3540 3785 200
Size: 904 478 271 314 360 1013 245 1157
Fragments arranged by size:
1157 1013 904 478 360 314 271 245
NaeI GCC'GGC
Cuts at: 679 2923 679
Size: 2244 2498
NarI GG'CG_CC
Cuts at: 241 701 2173 2421 241
Size: 460 1472 248 2562
Fragments arranged by size:
2562 1472 460 248
NgoMIV G'CCGG_C
Cuts at: 677 2921 677
Size: 2244 2498
NspI r_CATG'y
Cuts at: 927 1073 1795 2295 3202 927
Size: 146 722 500 907 2467
Fragments arranged by size:
2467 907 722 500 146
PciI A'CATG_T
Cuts at: 3198 3198
Size: 4742
PfoI T'CCnGG_A
Cuts at: 389 458 389
Size: 69 4673
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 928 960 3881 3913 928
Size: 32 2921 32 1757
Fragments arranged by size:
2921 1757 32 32
PpuMI rG'GwC_Cy
Cuts at: 393 456 393
Size: 63 4679
PstI C_TGCA'G
Cuts at: 632 812 1109 1789 2474 632
Size: 180 297 680 685 2900
Fragments arranged by size:
2900 685 680 297 180
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 1104 1582 2167 2527 1104
Size: 478 585 360 3319
Fragments arranged by size:
3319 585 478 360
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 734 1354 1756 734
Size: 620 402 3720
Fragments arranged by size:
3720 620 402
SalI G'TCGA_C
Cuts at: 1779 1779
Size: 4742
SapI GCTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
SbfI CC_TGCA'GG
Cuts at: 632 1789 632
Size: 1157 3585
ScaI AGT'ACT
Cuts at: 4259 4259
Size: 4742
SfcI C'TryA_G
Cuts at: 33 204 628 808 1105 1785 2470 3463 3654
Size: 171 424 180 297 680 685 993 191
Cuts at: 3654 33
Size: 1121
Fragments arranged by size:
1121 993 685 680 424 297 191 180
171
SfoI GGC'GCC
Cuts at: 242 702 2174 2422 242
Size: 460 1472 248 2562
Fragments arranged by size:
2562 1472 460 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SgrAI Cr'CCGG_yG
Cuts at: 243 243
Size: 4742
SmaI CCC'GGG
Cuts at: 175 1764 175
Size: 1589 3153
SmlI C'TyrA_G
Cuts at: 355 733 880 1042 1897 2812 3304 3566 355
Size: 378 147 162 855 915 492 262 1531
Fragments arranged by size:
1531 915 855 492 378 262 162 147
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4742
SphI G_CATG'C
Cuts at: 1073 1795 2295 1073
Size: 722 500 3520
Fragments arranged by size:
3520 722 500
StyI C'CwwG_G
Cuts at: 1010 1490 1505 1601 1876 2230 1010
Size: 480 15 96 275 354 3522
Fragments arranged by size:
3522 480 354 275 96 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 985 2325 2458 2917 3126 985
Size: 1340 133 459 209 2601
Fragments arranged by size:
2601 1340 459 209 133
TatI w'GTAC_w
Cuts at: 4257 4257
Size: 4742
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4259 4291 4259
Size: 32 4710
Tth111I GACn'n_nGTC
Cuts at: 2539 2539
Size: 4742
XbaI T'CTAG_A
Cuts at: 1773 1773
Size: 4742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2237 2237
Size: 4742
XhoI C'TCGA_G
Cuts at: 733 733
Size: 4742
XmaI C'CCGG_G
Cuts at: 173 1762 173
Size: 1589 3153
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BsgI BsiWI BsmI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XmnI ZraI