Restriction map for
pF1KB8147
(Circular) (Six-base) MAPSORT of: pF1KB8147.seq Check: 6177 from: 1 to: 3917
pF1KB8147 3917 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 122 156 836 955 122
Size: 34 680 119 3084
Fragments arranged by size:
3084 680 119 34
Acc65I G'GTAC_C
Cuts at: 933 933
Size: 3917
AclI AA'CG_TT
Cuts at: 3757 3757
Size: 3917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1742 2174 2921 3189 1742
Size: 432 747 268 2470
Fragments arranged by size:
2470 747 432 268
AflIII A'CryG_T
Cuts at: 1269 2373 3350 1269
Size: 1104 977 1836
Fragments arranged by size:
1836 1104 977
AgeI A'CCGG_T
Cuts at: 2305 2305
Size: 3917
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 244 276 244
Size: 32 3885
AlwNI CAG_nnn'CTG
Cuts at: 232 1222 2789 232
Size: 990 1567 1360
Fragments arranged by size:
1567 1360 990
ApaI G_GGCC'C
Cuts at: 889 889
Size: 3917
ApaLI G'TGCA_C
Cuts at: 2687 2687
Size: 3917
ApoI r'AATT_y
Cuts at: 292 424 921 3270 3526 292
Size: 132 497 2349 256 683
Fragments arranged by size:
2349 683 497 256 132
AseI AT'TA_AT
Cuts at: 20 20
Size: 3917
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
AvaI C'yCGr_G
Cuts at: 937 937
Size: 3917
BamHI G'GATC_C
Cuts at: 942 942
Size: 3917
BanI G'GyrC_C
Cuts at: 933 1347 1595 1630 933
Size: 414 248 35 3220
Fragments arranged by size:
3220 414 248 35
BanII G_rGCy'C
Cuts at: 889 931 1961 889
Size: 42 1030 2845
Fragments arranged by size:
2845 1030 42
BbeI G_GCGC'C
Cuts at: 1351 1599 1351
Size: 248 3669
BbsI GAAGACnn'nnnn_
Cuts at: 737 737
Size: 3917
BciVI GTATCCnnnnn_n'
Cuts at: 1815 2576 3917 1815
Size: 761 1341 1815
Fragments arranged by size:
1815 1341 761
BclI T'GATC_A
Cuts at: 1437 1437
Size: 3917
BglII A'GATC_T
Cuts at: 1432 3469 1432
Size: 2037 1880
BlpI GC'TnA_GC
Cuts at: 302 1029 302
Size: 727 3190
Bme1580I G_kGCm'C
Cuts at: 889 1542 1635 2691 889
Size: 653 93 1056 2115
Fragments arranged by size:
2115 1056 653 93
BmrI ACTGGGnnnn_n'
Cuts at: 1129 1210 1545 3689 1129
Size: 81 335 2144 1357
Fragments arranged by size:
2144 1357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 3917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 521
Size: 3917
Bpu10I CC'TnA_GC
Cuts at: 225 225
Size: 3917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 1972 2464 2762 1093
Size: 879 492 298 2248
Fragments arranged by size:
2248 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3621 3621
Size: 3917
BsaAI yAC'GTr
Cuts at: 1270 1270
Size: 3917
BsaBI GATnn'nnATC
Cuts at: 6 1431 3468 6
Size: 1425 2037 455
Fragments arranged by size:
2037 1425 455
BsaHI Gr'CG_yC
Cuts at: 1348 1596 2298 3794 1348
Size: 248 702 1496 1471
Fragments arranged by size:
1496 1471 702 248
BsaWI w'CCGG_w
Cuts at: 1106 1627 2305 2579 2726 3137 1106
Size: 521 678 274 147 411 1886
Fragments arranged by size:
1886 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 910 910
Size: 3917
BseYI C'CCAG_C
Cuts at: 1342 2100 2677 3173 1342
Size: 758 577 496 2086
Fragments arranged by size:
2086 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 599 599
Size: 3917
BsiEI CG_ry'CG
Cuts at: 104 1459 1505 2713 104
Size: 1355 46 1208 1308
Fragments arranged by size:
1355 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 226 931 1709 1899 2691 226
Size: 705 778 190 792 1452
Fragments arranged by size:
1452 792 778 705 190
BsmBI CGTCTCn'nnnn_
Cuts at: 668 668
Size: 3917
Bsp1286I G_dGCh'C
Cuts at: 226 889 931 1542 1635 1709 1899 1961 2691
Size: 663 42 611 93 74 190 62 730
Cuts at: 2691 226
Size: 1452
Fragments arranged by size:
1452 730 663 611 190 93 74 62
42
BspEI T'CCGG_A
Cuts at: 3137 3137
Size: 3917
BspHI T'CATG_A
Cuts at: 3093 3093
Size: 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 337 693 967 1181 1483 1864 337
Size: 356 274 214 302 381 2390
Fragments arranged by size:
2390 381 356 302 274 214
BsrBI CCG'CTC
Cuts at: 50 2209 2263 3747 3912 50
Size: 2159 54 1484 165 55
Fragments arranged by size:
2159 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 272 1829 272
Size: 1557 2360
BsrFI r'CCGG_y
Cuts at: 1915 2096 2305 1915
Size: 181 209 3527
Fragments arranged by size:
3527 209 181
BssSI C'ACGA_G
Cuts at: 313 2188 2546 313
Size: 1875 358 1684
Fragments arranged by size:
1875 1684 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1294 1294
Size: 3917
BstBI TT'CG_AA
Cuts at: 2278 2278
Size: 3917
BstYI r'GATC_y
Cuts at: 1 942 1409 1432 1767 2013 3025 3111 3123
Size: 941 467 23 335 246 1012 86 12
Cuts at: 3123 3469 1
Size: 346 449
Fragments arranged by size:
1012 941 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 123 123
Size: 3917
Bsu36I CC'TnA_GG
Cuts at: 626 626
Size: 3917
BtgI C'CryG_G
Cuts at: 107 107
Size: 3917
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1867 2048 2211 1867
Size: 181 163 3573
Fragments arranged by size:
3573 181 163
BtsI GCAGTG_nn'
Cuts at: 119 521 582 1160 119
Size: 402 61 578 2876
Fragments arranged by size:
2876 578 402 61
DraI TTT'AAA
Cuts at: 916 916
Size: 3917
DrdI GACnn_nn'nnGTC
Cuts at: 1623 2481 1623
Size: 858 3059
EaeI y'GGCC_r
Cuts at: 1502 1676 2067 2094 3439 1502
Size: 174 391 27 1345 1980
Fragments arranged by size:
1980 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1502 1502
Size: 3917
EarI CTCTTCn'nnn_
Cuts at: 899 1940 2150 899
Size: 1041 210 2666
Fragments arranged by size:
2666 1041 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2125 2435 2581 3727 2125
Size: 310 146 1146 2315
Fragments arranged by size:
2315 1146 310 146
EcoICRI GAG'CTC
Cuts at: 929 929
Size: 3917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 1742 2174 2921 3189 521
Size: 1221 432 747 268 1249
Fragments arranged by size:
1249 1221 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 878 878
Size: 3917
EcoO109I rG'GnC_Cy
Cuts at: 885 1056 885
Size: 171 3746
EcoRI G'AATT_C
Cuts at: 424 921 424
Size: 497 3420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1936 1936
Size: 3917
FspI TGC'GCA
Cuts at: 1698 1698
Size: 3917
HaeII r_GCGC'y
Cuts at: 1351 1599 2621 3600 1351
Size: 248 1022 979 1668
Fragments arranged by size:
1668 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1759 1789 1791 1821 1759
Size: 30 2 30 3855
Fragments arranged by size:
3855 30 30 2
HincII GTy'rAC
Cuts at: 157 369 708 956 157
Size: 212 339 248 3118
Fragments arranged by size:
3118 339 248 212
KasI G'GCGC_C
Cuts at: 1347 1595 1347
Size: 248 3669
KpnI G_GTAC'C
Cuts at: 937 937
Size: 3917
MluI A'CGCG_T
Cuts at: 3350 3350
Size: 3917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2588 2772 2588
Size: 184 3733
MscI TGG'CCA
Cuts at: 1678 1678
Size: 3917
MslI CAynn'nnrTG
Cuts at: 112 1906 2033 112
Size: 1794 127 1996
Fragments arranged by size:
1996 1794 127
MspA1I CmG'CkG
Cuts at: 616 688 1028 1342 1702 2715 2960 616
Size: 72 340 314 360 1013 245 1573
Fragments arranged by size:
1573 1013 360 340 314 245 72
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 3917
NarI GG'CG_CC
Cuts at: 1348 1596 1348
Size: 248 3669
NcoI C'CATG_G
Cuts at: 107 107
Size: 3917
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 3917
NspI r_CATG'y
Cuts at: 168 258 970 1470 2377 168
Size: 90 712 500 907 1708
Fragments arranged by size:
1708 907 712 500 90
PciI A'CATG_T
Cuts at: 2373 2373
Size: 3917
PmeI GTTT'AAAC
Cuts at: 916 916
Size: 3917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 109 141 3056 3088 109
Size: 32 2915 32 938
Fragments arranged by size:
2915 938 32 32
PspOMI G'GGCC_C
Cuts at: 885 885
Size: 3917
PstI C_TGCA'G
Cuts at: 194 516 964 1649 194
Size: 322 448 685 2462
Fragments arranged by size:
2462 685 448 322
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3917
PvuII CAG'CTG
Cuts at: 688 1342 1702 688
Size: 654 360 2903
Fragments arranged by size:
2903 654 360
RsrII CG'GwC_CG
Cuts at: 2112 2112
Size: 3917
SacI G_AGCT'C
Cuts at: 931 931
Size: 3917
SalI G'TCGA_C
Cuts at: 155 954 155
Size: 799 3118
SapI GCTCTTCn'nnn_
Cuts at: 1940 2150 1940
Size: 210 3707
SbfI CC_TGCA'GG
Cuts at: 964 964
Size: 3917
ScaI AGT'ACT
Cuts at: 3434 3434
Size: 3917
SfcI C'TryA_G
Cuts at: 33 190 512 960 1645 2638 2829 33
Size: 157 322 448 685 993 191 1121
Fragments arranged by size:
1121 993 685 448 322 191 157
SfoI GGC'GCC
Cuts at: 1349 1597 1349
Size: 248 3669
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
SmaI CCC'GGG
Cuts at: 939 939
Size: 3917
SmlI C'TyrA_G
Cuts at: 1072 1987 2479 2741 1072
Size: 915 492 262 2248
Fragments arranged by size:
2248 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3917
SphI G_CATG'C
Cuts at: 970 1470 970
Size: 500 3417
StyI C'CwwG_G
Cuts at: 107 1051 1405 107
Size: 944 354 2619
Fragments arranged by size:
2619 944 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 352 1500 1633 2092 2301 352
Size: 1148 133 459 209 1968
Fragments arranged by size:
1968 1148 459 209 133
TatI w'GTAC_w
Cuts at: 317 3432 317
Size: 3115 802
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 3885
Tth111I GACn'n_nGTC
Cuts at: 500 1714 500
Size: 1214 2703
XbaI T'CTAG_A
Cuts at: 948 948
Size: 3917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1412 1412
Size: 3917
XmaI C'CCGG_G
Cuts at: 937 937
Size: 3917
XmnI GAAnn'nnTTC
Cuts at: 428 448 428
Size: 20 3897
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsrGI BssHII BstEII BstXI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI