Restriction map for
pF1KB8146
(Circular) (Six-base) MAPSORT of: pF1KB8146.seq Check: 7894 from: 1 to: 4232
pF1KB8146 4232 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1270 1270
Size: 4232
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 565 811 2057 2489 3236 3504 493
Size: 72 246 1246 432 747 268 1221
Fragments arranged by size:
1246 1221 747 432 268 246 72
AfeI AGC'GCT
Cuts at: 588 588
Size: 4232
AflIII A'CryG_T
Cuts at: 1584 2688 3665 1584
Size: 1104 977 2151
Fragments arranged by size:
2151 1104 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AhdI GACnn_n'nnGTC
Cuts at: 1167 1167
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 178 777 1135 1537 3104 178
Size: 599 358 402 1567 1306
Fragments arranged by size:
1567 1306 599 402 358
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 1236 3585 3841 1236
Size: 2349 256 1627
Fragments arranged by size:
2349 1627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 230 1252 230
Size: 1022 3210
BamHI G'GATC_C
Cuts at: 264 1257 264
Size: 993 3239
BanI G'GyrC_C
Cuts at: 254 1248 1662 1910 1945 254
Size: 994 414 248 35 2541
Fragments arranged by size:
2541 994 414 248 35
BanII G_rGCy'C
Cuts at: 1246 2276 1246
Size: 1030 3202
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 415 449 415
Size: 34 4198
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BfrBI ATG'CAT
Cuts at: 679 679
Size: 4232
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 806 1344 806
Size: 538 3694
Bme1580I G_kGCm'C
Cuts at: 257 1857 1950 3006 257
Size: 1600 93 1056 1483
Fragments arranged by size:
1600 1483 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 495 1041 1444 1525 1860 4004 495
Size: 546 403 81 335 2144 723
Fragments arranged by size:
2144 723 546 403 335 81
BmtI G_CTAG'C
Cuts at: 304 304
Size: 4232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1141 2599 1141
Size: 1458 2774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 316
Size: 4232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 1408 2287 2779 3077 271
Size: 1137 879 492 298 1426
Fragments arranged by size:
1426 1137 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3936 3936
Size: 4232
BsaAI yAC'GTr
Cuts at: 1585 1585
Size: 4232
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 1663 1911 2613 4109 1663
Size: 248 702 1496 1786
Fragments arranged by size:
1786 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 473 503 473
Size: 30 4202
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 192 841 192
Size: 649 3583
BseYI C'CCAG_C
Cuts at: 171 872 1657 2415 2992 3488 171
Size: 701 785 758 577 496 915
Fragments arranged by size:
915 785 758 701 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 597 597
Size: 4232
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1246 2024 2214 3006 1246
Size: 778 190 792 2472
Fragments arranged by size:
2472 792 778 190
BsmI GAATG_Cn'
Cuts at: 681 681
Size: 4232
Bsp1286I G_dGCh'C
Cuts at: 257 1246 1857 1950 2024 2214 2276 3006 257
Size: 989 611 93 74 190 62 730 1483
Fragments arranged by size:
1483 989 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 201 1282 1496 1798 2179 201
Size: 1081 214 302 381 2254
Fragments arranged by size:
2254 1081 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2524 2578 4062 4227 50
Size: 2474 54 1484 165 55
Fragments arranged by size:
2474 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 465 2144 465
Size: 1679 2553
BsrFI r'CCGG_y
Cuts at: 2230 2411 2620 2230
Size: 181 209 3842
Fragments arranged by size:
3842 209 181
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 1609 1609
Size: 4232
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstEII G'GTnAC_C
Cuts at: 954 954
Size: 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 485 747 485
Size: 262 3970
BstYI r'GATC_y
Cuts at: 1 264 1017 1257 1724 1747 2082 2328 3340
Size: 263 753 240 467 23 335 246 1012
Cuts at: 3340 3426 3438 3784 1
Size: 86 12 346 449
Fragments arranged by size:
1012 753 467 449 346 335 263 246
240 86 23 12
BtgI C'CryG_G
Cuts at: 258 877 950 1223 258
Size: 619 73 273 3267
Fragments arranged by size:
3267 619 273 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2182 2363 2526 2182
Size: 181 163 3888
Fragments arranged by size:
3888 181 163
BtsI GCAGTG_nn'
Cuts at: 160 1475 160
Size: 1315 2917
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DraIII CAC_nnn'GTG
Cuts at: 1100 1100
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 794 1938 2796 794
Size: 1144 858 2230
Fragments arranged by size:
2230 1144 858
EaeI y'GGCC_r
Cuts at: 971 1817 1991 2382 2409 3754 971
Size: 846 174 391 27 1345 1449
Fragments arranged by size:
1449 1345 846 391 174 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 584 787 818 2255 2465 584
Size: 203 31 1437 210 2351
Fragments arranged by size:
2351 1437 210 203 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 207 537 1102 2440 2750 2896 4042 207
Size: 330 565 1338 310 146 1146 397
Fragments arranged by size:
1338 1146 565 397 330 310 146
EcoICRI GAG'CTC
Cuts at: 1244 1244
Size: 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 493 565 811 2057 2489 3236 3504 316
Size: 177 72 246 1246 432 747 268 1044
Fragments arranged by size:
1246 1044 747 432 268 246 177 72
EcoO109I rG'GnC_Cy
Cuts at: 1149 1371 1149
Size: 222 4010
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
EcoRV GAT'ATC
Cuts at: 768 768
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 244 590 1666 1914 2936 3915 244
Size: 346 1076 248 1022 979 561
Fragments arranged by size:
1076 1022 979 561 346 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 308 340 752 784 1153 1185 2074 2104 2106
Size: 32 412 32 369 32 889 30 2
Cuts at: 2106 2136 308
Size: 30 2404
Fragments arranged by size:
2404 889 412 369 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1271 1271
Size: 4232
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 396 707 2903 3087 396
Size: 311 2196 184 1541
Fragments arranged by size:
2196 1541 311 184
MscI TGG'CCA
Cuts at: 973 1993 973
Size: 1020 3212
MslI CAynn'nnrTG
Cuts at: 370 745 2221 2348 370
Size: 375 1476 127 2254
Fragments arranged by size:
2254 1476 375 127
MspA1I CmG'CkG
Cuts at: 689 1155 1343 1657 2017 3030 3275 689
Size: 466 188 314 360 1013 245 1646
Fragments arranged by size:
1646 1013 466 360 314 245 188
NaeI GCC'GGC
Cuts at: 2413 2413
Size: 4232
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NcoI C'CATG_G
Cuts at: 950 1223 950
Size: 273 3959
NgoMIV G'CCGG_C
Cuts at: 2411 2411
Size: 4232
NheI G'CTAG_C
Cuts at: 300 300
Size: 4232
NsiI A_TGCA'T
Cuts at: 681 681
Size: 4232
NspI r_CATG'y
Cuts at: 814 870 1285 1785 2692 814
Size: 56 415 500 907 2354
Fragments arranged by size:
2354 907 500 415 56
PasI CC'CwG_GG
Cuts at: 597 726 597
Size: 129 4103
PciI A'CATG_T
Cuts at: 2688 2688
Size: 4232
PflMI CCAn_nnn'nTGG
Cuts at: 178 178
Size: 4232
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 520 552 3371 3403 520
Size: 32 2819 32 1349
Fragments arranged by size:
2819 1349 32 32
PpuMI rG'GwC_Cy
Cuts at: 1149 1149
Size: 4232
PstI C_TGCA'G
Cuts at: 121 653 1160 1279 1964 121
Size: 532 507 119 685 2389
Fragments arranged by size:
2389 685 532 507 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 1657 2017 1657
Size: 360 3872
RsrII CG'GwC_CG
Cuts at: 524 2427 524
Size: 1903 2329
SacI G_AGCT'C
Cuts at: 1246 1246
Size: 4232
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SfcI C'TryA_G
Cuts at: 33 117 621 649 1156 1275 1960 2953 3144
Size: 84 504 28 507 119 685 993 191
Cuts at: 3144 33
Size: 1121
Fragments arranged by size:
1121 993 685 507 504 191 119 84
28
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 232 1254 232
Size: 1022 3210
SmlI C'TyrA_G
Cuts at: 286 1387 2302 2794 3056 286
Size: 1101 915 492 262 1462
Fragments arranged by size:
1462 1101 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 1285 1785 1285
Size: 500 3732
StyI C'CwwG_G
Cuts at: 272 378 950 1175 1223 1366 1720 272
Size: 106 572 225 48 143 354 2784
Fragments arranged by size:
2784 572 354 225 143 106 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 477 541 589 1815 1948 2407 2616 477
Size: 64 48 1226 133 459 209 2093
Fragments arranged by size:
2093 1226 459 209 133 64 48
TatI w'GTAC_w
Cuts at: 3747 3747
Size: 4232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 414 446 473 505 3749 3781 414
Size: 32 27 32 3244 32 865
Fragments arranged by size:
3244 865 32 32 32 27
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 690 1727 690
Size: 1037 3195
XmaI C'CCGG_G
Cuts at: 230 1252 230
Size: 1022 3210
XmnI GAAnn'nnTTC
Cuts at: 853 1195 853
Size: 342 3890
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BglI BmgBI Bpu10I BsiWI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
PacI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI