Restriction map for
pF1KB8136
(Circular) (Six-base) MAPSORT of: pF1KB8136.seq Check: 83 from: 1 to: 4412
pF1KB8136 4412 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1167 1167
Size: 4412
AccI GT'mk_AC
Cuts at: 1450 1450
Size: 4412
Acc65I G'GTAC_C
Cuts at: 1428 1428
Size: 4412
AclI AA'CG_TT
Cuts at: 4252 4252
Size: 4412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 2237 2669 3416 3684 829
Size: 1408 432 747 268 1557
Fragments arranged by size:
1557 1408 747 432 268
AfeI AGC'GCT
Cuts at: 930 930
Size: 4412
AflIII A'CryG_T
Cuts at: 392 908 1319 1764 2868 3845 392
Size: 516 411 445 1104 977 959
Fragments arranged by size:
1104 977 959 516 445 411
AgeI A'CCGG_T
Cuts at: 2800 2800
Size: 4412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1042 1042
Size: 4412
AlwNI CAG_nnn'CTG
Cuts at: 1155 1227 1252 1717 3284 1155
Size: 72 25 465 1567 2283
Fragments arranged by size:
2283 1567 465 72 25
ApaI G_GGCC'C
Cuts at: 1196 1196
Size: 4412
ApaLI G'TGCA_C
Cuts at: 572 1017 3182 572
Size: 445 2165 1802
Fragments arranged by size:
2165 1802 445
ApoI r'AATT_y
Cuts at: 275 1052 1416 3765 4021 275
Size: 777 364 2349 256 666
Fragments arranged by size:
2349 777 666 364 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4412
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
AvaI C'yCGr_G
Cuts at: 385 712 794 1432 385
Size: 327 82 638 3365
Fragments arranged by size:
3365 638 327 82
BamHI G'GATC_C
Cuts at: 1437 1437
Size: 4412
BanI G'GyrC_C
Cuts at: 1428 1842 2090 2125 1428
Size: 414 248 35 3715
Fragments arranged by size:
3715 414 248 35
BanII G_rGCy'C
Cuts at: 1196 1426 2456 1196
Size: 230 1030 3152
Fragments arranged by size:
3152 1030 230
BbeI G_GCGC'C
Cuts at: 1846 2094 1846
Size: 248 4164
BbsI GAAGACnn'nnnn_
Cuts at: 1004 1383 1004
Size: 379 4033
BbvCI CC'TCA_GC
Cuts at: 925 925
Size: 4412
BciVI GTATCCnnnnn_n'
Cuts at: 2310 3071 4412 2310
Size: 761 1341 2310
Fragments arranged by size:
2310 1341 761
BclI T'GATC_A
Cuts at: 1932 1932
Size: 4412
BglII A'GATC_T
Cuts at: 1927 3964 1927
Size: 2037 2375
BlpI GC'TnA_GC
Cuts at: 1183 1524 1183
Size: 341 4071
Bme1580I G_kGCm'C
Cuts at: 576 860 1021 1196 2037 2130 3186 576
Size: 284 161 175 841 93 1056 1802
Fragments arranged by size:
1802 1056 841 284 175 161 93
BmgBI CAC'GTC
Cuts at: 537 726 1269 537
Size: 189 543 3680
Fragments arranged by size:
3680 543 189
BmrI ACTGGGnnnn_n'
Cuts at: 560 1240 1297 1624 1705 2040 4184 560
Size: 680 57 327 81 335 2144 788
Fragments arranged by size:
2144 788 680 335 327 81 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 4412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1422 1422
Size: 4412
Bpu10I CC'TnA_GC
Cuts at: 925 925
Size: 4412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1588 2467 2959 3257 1588
Size: 879 492 298 2743
Fragments arranged by size:
2743 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 184 238 1060 4116 184
Size: 54 822 3056 480
Fragments arranged by size:
3056 822 480 54
BsaAI yAC'GTr
Cuts at: 369 395 1765 369
Size: 26 1370 3016
Fragments arranged by size:
3016 1370 26
BsaBI GATnn'nnATC
Cuts at: 6 1926 3963 6
Size: 1920 2037 455
Fragments arranged by size:
2037 1920 455
BsaHI Gr'CG_yC
Cuts at: 1843 2091 2793 4289 1843
Size: 248 702 1496 1966
Fragments arranged by size:
1966 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1601 2122 2800 3074 3221 3632 1601
Size: 521 678 274 147 411 2381
Fragments arranged by size:
2381 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1256 1286 1373 1403 1256
Size: 30 87 30 4265
Fragments arranged by size:
4265 87 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 281 220
Size: 61 4351
BseYI C'CCAG_C
Cuts at: 248 1337 1837 2595 3172 3668 248
Size: 1089 500 758 577 496 992
Fragments arranged by size:
1089 992 758 577 500 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 1099 186
Size: 913 3499
BsiEI CG_ry'CG
Cuts at: 104 1954 2000 3208 104
Size: 1850 46 1208 1308
Fragments arranged by size:
1850 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 576 896 1021 1184 1426 2204 2394 3186 576
Size: 320 125 163 242 778 190 792 1802
Fragments arranged by size:
1802 792 778 320 242 190 163 125
BsiWI C'GTAC_G
Cuts at: 911 911
Size: 4412
BsmBI CGTCTCn'nnnn_
Cuts at: 1259 1259
Size: 4412
Bsp1286I G_dGCh'C
Cuts at: 576 860 896 1021 1184 1196 1426 2037 2130
Size: 284 36 125 163 12 230 611 93
Cuts at: 2130 2204 2394 2456 3186 576
Size: 74 190 62 730 1802
Fragments arranged by size:
1802 730 611 284 230 190 163 125
93 74 62 36 12
BspEI T'CCGG_A
Cuts at: 3632 3632
Size: 4412
BspHI T'CATG_A
Cuts at: 770 1232 3588 770
Size: 462 2356 1594
Fragments arranged by size:
2356 1594 462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 760 1167 1462 1676 1978 2359 760
Size: 407 295 214 302 381 2813
Fragments arranged by size:
2813 407 381 302 295 214
BsrBI CCG'CTC
Cuts at: 50 595 2704 2758 4242 4407 50
Size: 545 2109 54 1484 165 55
Fragments arranged by size:
2109 1484 545 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2324 2324
Size: 4412
BsrFI r'CCGG_y
Cuts at: 2410 2591 2800 2410
Size: 181 209 4022
Fragments arranged by size:
4022 209 181
BssSI C'ACGA_G
Cuts at: 2683 3041 2683
Size: 358 4054
BstAPI GCAn_nnn'nTGC
Cuts at: 580 855 1789 580
Size: 275 934 3203
Fragments arranged by size:
3203 934 275
BstBI TT'CG_AA
Cuts at: 2773 2773
Size: 4412
BstXI CCAn_nnnn'nTGG
Cuts at: 621 621
Size: 4412
BstYI r'GATC_y
Cuts at: 1 1437 1904 1927 2262 2508 3520 3606 3618
Size: 1436 467 23 335 246 1012 86 12
Cuts at: 3618 3964 1
Size: 346 449
Fragments arranged by size:
1436 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1072 1072
Size: 4412
BtgI C'CryG_G
Cuts at: 107 836 107
Size: 729 3683
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 204 2362 2543 2706 204
Size: 2158 181 163 1910
Fragments arranged by size:
2158 1910 181 163
BtsI GCAGTG_nn'
Cuts at: 174 296 870 1655 174
Size: 122 574 785 2931
Fragments arranged by size:
2931 785 574 122
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 338 373 338
Size: 35 4377
DraI TTT'AAA
Cuts at: 1411 1411
Size: 4412
DraIII CAC_nnn'GTG
Cuts at: 855 855
Size: 4412
DrdI GACnn_nn'nnGTC
Cuts at: 1246 2118 2976 1246
Size: 872 858 2682
Fragments arranged by size:
2682 872 858
EaeI y'GGCC_r
Cuts at: 215 1997 2171 2562 2589 3934 215
Size: 1782 174 391 27 1345 693
Fragments arranged by size:
1782 1345 693 391 174 27
EagI C'GGCC_G
Cuts at: 1997 1997
Size: 4412
EarI CTCTTCn'nnn_
Cuts at: 146 401 571 901 2435 2645 146
Size: 255 170 330 1534 210 1913
Fragments arranged by size:
1913 1534 330 255 210 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2620 2930 3076 4222 2620
Size: 310 146 1146 2810
Fragments arranged by size:
2810 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1424 1424
Size: 4412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1422 2237 2669 3416 3684 829
Size: 593 815 432 747 268 1557
Fragments arranged by size:
1557 815 747 593 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 187 1159 187
Size: 972 3440
EcoO109I rG'GnC_Cy
Cuts at: 749 1109 1193 1551 749
Size: 360 84 358 3610
Fragments arranged by size:
3610 360 358 84
EcoRI G'AATT_C
Cuts at: 1416 1416
Size: 4412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 912 2431 912
Size: 1519 2893
FspI TGC'GCA
Cuts at: 2193 2193
Size: 4412
HaeII r_GCGC'y
Cuts at: 932 1846 2094 3116 4095 932
Size: 914 248 1022 979 1249
Fragments arranged by size:
1249 1022 979 914 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 546 578 991 1023 1256 1288 1351 1373 1383
Size: 32 413 32 233 32 63 22 10
Cuts at: 1383 1405 2254 2284 2286 2316 546
Size: 22 849 30 2 30 2642
Fragments arranged by size:
2642 849 413 233 63 32 32 32
30 30 22 22 10 2
HincII GTy'rAC
Cuts at: 1451 1451
Size: 4412
KasI G'GCGC_C
Cuts at: 1842 2090 1842
Size: 248 4164
KpnI G_GTAC'C
Cuts at: 1432 1432
Size: 4412
MluI A'CGCG_T
Cuts at: 908 3845 908
Size: 2937 1475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1013 1359 3083 3267 1013
Size: 346 1724 184 2158
Fragments arranged by size:
2158 1724 346 184
MscI TGG'CCA
Cuts at: 217 2173 217
Size: 1956 2456
MslI CAynn'nnrTG
Cuts at: 371 676 1324 2401 2528 371
Size: 305 648 1077 127 2255
Fragments arranged by size:
2255 1077 648 305 127
MspA1I CmG'CkG
Cuts at: 642 1523 1837 2197 3210 3455 642
Size: 881 314 360 1013 245 1599
Fragments arranged by size:
1599 1013 881 360 314 245
NaeI GCC'GGC
Cuts at: 2593 2593
Size: 4412
NarI GG'CG_CC
Cuts at: 1843 2091 1843
Size: 248 4164
NcoI C'CATG_G
Cuts at: 107 836 107
Size: 729 3683
NgoMIV G'CCGG_C
Cuts at: 2591 2591
Size: 4412
NspI r_CATG'y
Cuts at: 1323 1465 1965 2872 1323
Size: 142 500 907 2863
Fragments arranged by size:
2863 907 500 142
PciI A'CATG_T
Cuts at: 1319 2868 1319
Size: 1549 2863
PflMI CCAn_nnn'nTGG
Cuts at: 1214 1214
Size: 4412
PfoI T'CCnGG_A
Cuts at: 530 530
Size: 4412
PmeI GTTT'AAAC
Cuts at: 1411 1411
Size: 4412
PmlI CAC'GTG
Cuts at: 369 395 369
Size: 26 4386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4380
PpuMI rG'GwC_Cy
Cuts at: 749 1109 749
Size: 360 4052
PspOMI G'GGCC_C
Cuts at: 1192 1192
Size: 4412
PstI C_TGCA'G
Cuts at: 188 303 500 653 865 1160 1459 2144 188
Size: 115 197 153 212 295 299 685 2456
Fragments arranged by size:
2456 685 299 295 212 197 153 115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4412
PvuII CAG'CTG
Cuts at: 642 1837 2197 642
Size: 1195 360 2857
Fragments arranged by size:
2857 1195 360
RsrII CG'GwC_CG
Cuts at: 2607 2607
Size: 4412
SacI G_AGCT'C
Cuts at: 1426 1426
Size: 4412
SalI G'TCGA_C
Cuts at: 1449 1449
Size: 4412
SanDI GG'GwC_CC
Cuts at: 1109 1109
Size: 4412
SapI GCTCTTCn'nnn_
Cuts at: 146 901 2435 2645 146
Size: 755 1534 210 1913
Fragments arranged by size:
1913 1534 755 210
SbfI CC_TGCA'GG
Cuts at: 188 1160 1459 188
Size: 972 299 3141
Fragments arranged by size:
3141 972 299
ScaI AGT'ACT
Cuts at: 412 3929 412
Size: 3517 895
SfcI C'TryA_G
Cuts at: 33 184 299 496 649 861 1156 1455 2140
Size: 151 115 197 153 212 295 299 685
Cuts at: 2140 3133 3324 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 299 295 212 197 191
153 151 115
SfoI GGC'GCC
Cuts at: 1844 2092 1844
Size: 248 4164
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
SmaI CCC'GGG
Cuts at: 796 1434 796
Size: 638 3774
SmlI C'TyrA_G
Cuts at: 1567 2482 2974 3236 1567
Size: 915 492 262 2743
Fragments arranged by size:
2743 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4412
SphI G_CATG'C
Cuts at: 1465 1965 1465
Size: 500 3912
StyI C'CwwG_G
Cuts at: 107 836 1546 1900 107
Size: 729 710 354 2619
Fragments arranged by size:
2619 729 710 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1995 2128 2587 2796 1995
Size: 133 459 209 3611
Fragments arranged by size:
3611 459 209 133
TatI w'GTAC_w
Cuts at: 195 410 3927 195
Size: 215 3517 680
Fragments arranged by size:
3517 680 215
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3929 3961 3929
Size: 32 4380
Tth111I GACn'n_nGTC
Cuts at: 820 1246 1270 2209 820
Size: 426 24 939 3023
Fragments arranged by size:
3023 939 426 24
XbaI T'CTAG_A
Cuts at: 1062 1443 1062
Size: 381 4031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1907 1907
Size: 4412
XmaI C'CCGG_G
Cuts at: 794 1432 794
Size: 638 3774
XmnI GAAnn'nnTTC
Cuts at: 275 275
Size: 4412
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BaeI
BcgI BfrBI BglI BmtI BsmI BsrGI BssHII BstEII
BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI