Restriction map for
pF1KB8133
(Circular) (Six-base) MAPSORT of: pF1KB8133.seq Check: 7203 from: 1 to: 4811
pF1KB8133 4811 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 1544 1544
Size: 4811
AccI GT'mk_AC
Cuts at: 149 1849 149
Size: 1700 3111
Acc65I G'GTAC_C
Cuts at: 419 1352 1827 419
Size: 933 475 3403
Fragments arranged by size:
3403 933 475
AclI AA'CG_TT
Cuts at: 4651 4651
Size: 4811
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1644 2636 3068 3815 4083 1644
Size: 992 432 747 268 2372
Fragments arranged by size:
2372 992 747 432 268
AflIII A'CryG_T
Cuts at: 677 2163 3267 4244 677
Size: 1486 1104 977 1244
Fragments arranged by size:
1486 1244 1104 977
AgeI A'CCGG_T
Cuts at: 3199 3199
Size: 4811
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 145 145
Size: 4811
AlwNI CAG_nnn'CTG
Cuts at: 333 369 393 1218 1680 2116 3683 333
Size: 36 24 825 462 436 1567 1461
Fragments arranged by size:
1567 1461 825 462 436 36 24
ApaI G_GGCC'C
Cuts at: 175 1247 175
Size: 1072 3739
ApaLI G'TGCA_C
Cuts at: 3581 3581
Size: 4811
ApoI r'AATT_y
Cuts at: 1815 4164 4420 1815
Size: 2349 256 2206
Fragments arranged by size:
2349 2206 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4811
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4811
AvaI C'yCGr_G
Cuts at: 573 714 1123 1831 573
Size: 141 409 708 3553
Fragments arranged by size:
3553 708 409 141
BamHI G'GATC_C
Cuts at: 1836 1836
Size: 4811
BanI G'GyrC_C
Cuts at: 345 419 1141 1352 1752 1827 2241 2489 2524
Size: 74 722 211 400 75 414 248 35
Cuts at: 2524 345
Size: 2632
Fragments arranged by size:
2632 722 414 400 248 211 75 74
35
BanII G_rGCy'C
Cuts at: 175 1247 1270 1485 1825 2855 175
Size: 1072 23 215 340 1030 2131
Fragments arranged by size:
2131 1072 1030 340 215 23
BbeI G_GCGC'C
Cuts at: 1145 2245 2493 1145
Size: 1100 248 3463
Fragments arranged by size:
3463 1100 248
BbsI GAAGACnn'nnnn_
Cuts at: 1650 1650
Size: 4811
BbvCI CC'TCA_GC
Cuts at: 400 400
Size: 4811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 867 901 1114 1148 1536 1570 867
Size: 34 213 34 388 34 4108
Fragments arranged by size:
4108 388 213 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2709 3470 4811 2709
Size: 761 1341 2709
Fragments arranged by size:
2709 1341 761
BclI T'GATC_A
Cuts at: 2331 2331
Size: 4811
BglI GCCn_nnn'nGGC
Cuts at: 1567 1567
Size: 4811
BglII A'GATC_T
Cuts at: 155 458 2326 4363 155
Size: 303 1868 2037 603
Fragments arranged by size:
2037 1868 603 303
BlpI GC'TnA_GC
Cuts at: 1923 1923
Size: 4811
Bme1580I G_kGCm'C
Cuts at: 175 290 350 1247 2436 2529 3585 175
Size: 115 60 897 1189 93 1056 1401
Fragments arranged by size:
1401 1189 1056 897 115 93 60
BmgBI CAC'GTC
Cuts at: 183 285 678 929 1299 183
Size: 102 393 251 370 3695
Fragments arranged by size:
3695 393 370 251 102
BmrI ACTGGGnnnn_n'
Cuts at: 525 534 2023 2104 2439 4583 525
Size: 9 1489 81 335 2144 753
Fragments arranged by size:
2144 1489 753 335 81 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1499 3178 1499
Size: 1679 3132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 483 540 1057 1732 483
Size: 57 517 675 3562
Fragments arranged by size:
3562 675 517 57
Bpu10I CC'TnA_GC
Cuts at: 163 400 163
Size: 237 4574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 1987 2866 3358 3656 1441
Size: 546 879 492 298 2596
Fragments arranged by size:
2596 879 546 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 694 1072 4515 694
Size: 378 3443 990
Fragments arranged by size:
3443 990 378
BsaAI yAC'GTr
Cuts at: 1362 2164 1362
Size: 802 4009
BsaBI GATnn'nnATC
Cuts at: 6 1204 2325 4362 6
Size: 1198 1121 2037 455
Fragments arranged by size:
2037 1198 1121 455
BsaHI Gr'CG_yC
Cuts at: 1142 1541 1703 2242 2490 3192 4688 1142
Size: 399 162 539 248 702 1496 1265
Fragments arranged by size:
1496 1265 702 539 399 248 162
BsaWI w'CCGG_w
Cuts at: 2000 2521 3199 3473 3620 4031 2000
Size: 521 678 274 147 411 2780
Fragments arranged by size:
2780 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 591 982 1444 334
Size: 257 391 462 3701
Fragments arranged by size:
3701 462 391 257
BseYI C'CCAG_C
Cuts at: 297 1251 2236 2994 3571 4067 297
Size: 954 985 758 577 496 1041
Fragments arranged by size:
1041 985 954 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 382 432 475 709 379
Size: 3 50 43 234 4481
Fragments arranged by size:
4481 234 50 43 3
BsiEI CG_ry'CG
Cuts at: 104 179 2353 2399 3607 104
Size: 75 2174 46 1208 1308
Fragments arranged by size:
2174 1308 1208 75 46
BsiHKAI G_wGCw'C
Cuts at: 1825 2603 2793 3585 1825
Size: 778 190 792 3051
Fragments arranged by size:
3051 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 175 290 350 1247 1270 1485 1825 2436 2529
Size: 115 60 897 23 215 340 611 93
Cuts at: 2529 2603 2793 2855 3585 175
Size: 74 190 62 730 1401
Fragments arranged by size:
1401 897 730 611 340 215 190 115
93 74 62 60 23
BspEI T'CCGG_A
Cuts at: 4031 4031
Size: 4811
BspHI T'CATG_A
Cuts at: 3987 3987
Size: 4811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 731 908 1448 1861 2075 2377 2758 731
Size: 177 540 413 214 302 381 2784
Fragments arranged by size:
2784 540 413 381 302 214 177
BsrBI CCG'CTC
Cuts at: 50 3103 3157 4641 4806 50
Size: 3053 54 1484 165 55
Fragments arranged by size:
3053 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2723 2723
Size: 4811
BsrFI r'CCGG_y
Cuts at: 913 1577 2809 2990 3199 913
Size: 664 1232 181 209 2525
Fragments arranged by size:
2525 1232 664 209 181
BssSI C'ACGA_G
Cuts at: 1633 3082 3440 1633
Size: 1449 358 3004
Fragments arranged by size:
3004 1449 358
BstAPI GCAn_nnn'nTGC
Cuts at: 369 1218 1680 2188 369
Size: 849 462 508 2992
Fragments arranged by size:
2992 849 508 462
BstBI TT'CG_AA
Cuts at: 3172 3172
Size: 4811
BstEII G'GTnAC_C
Cuts at: 916 916
Size: 4811
BstYI r'GATC_y
Cuts at: 1 155 458 827 1214 1836 2303 2326 2661
Size: 154 303 369 387 622 467 23 335
Cuts at: 2661 2907 3919 4005 4017 4363 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 622 467 449 387 369 346 335
303 246 154 86 23 12
BtgI C'CryG_G
Cuts at: 107 1235 1762 107
Size: 1128 527 3156
Fragments arranged by size:
3156 1128 527
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2761 2942 3105 2761
Size: 181 163 4467
Fragments arranged by size:
4467 181 163
BtsI GCAGTG_nn'
Cuts at: 1137 2054 1137
Size: 917 3894
DraI TTT'AAA
Cuts at: 1810 1810
Size: 4811
DrdI GACnn_nn'nnGTC
Cuts at: 145 2517 3375 145
Size: 2372 858 1581
Fragments arranged by size:
2372 1581 858
EaeI y'GGCC_r
Cuts at: 176 188 302 869 923 1088 1397 1559 1619
Size: 12 114 567 54 165 309 162 60
Cuts at: 1619 1691 2396 2570 2961 2988 4333 176
Size: 72 705 174 391 27 1345 654
Fragments arranged by size:
1345 705 654 567 391 309 174 165
162 114 72 60 54 27 12
EagI C'GGCC_G
Cuts at: 176 2396 176
Size: 2220 2591
EarI CTCTTCn'nnn_
Cuts at: 2834 3044 2834
Size: 210 4601
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1585 3019 3329 3475 4621 1585
Size: 1434 310 146 1146 1775
Fragments arranged by size:
1775 1434 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1823 1823
Size: 4811
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 483 540 1057 1644 1732 2636 3068 3815 4083
Size: 57 517 587 88 904 432 747 268
Cuts at: 4083 483
Size: 1211
Fragments arranged by size:
1211 904 747 587 517 432 268 88
57
EcoO109I rG'GnC_Cy
Cuts at: 390 1243 1950 390
Size: 853 707 3251
Fragments arranged by size:
3251 853 707
EcoRI G'AATT_C
Cuts at: 1815 1815
Size: 4811
EcoRV GAT'ATC
Cuts at: 741 741
Size: 4811
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1026 2830 1026
Size: 1804 3007
FspI TGC'GCA
Cuts at: 1493 2592 1493
Size: 1099 3712
FspAI rTGC'GCAy
Cuts at: 1493 1493
Size: 4811
HaeII r_GCGC'y
Cuts at: 1145 1725 2245 2493 3515 4494 1145
Size: 580 520 248 1022 979 1462
Fragments arranged by size:
1462 1022 979 580 520 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 730 762 775 807 1307 1339 2653 2683 2685
Size: 32 13 32 500 32 1314 30 2
Cuts at: 2685 2715 730
Size: 30 2826
Fragments arranged by size:
2826 1314 500 32 32 32 30 30
13 2
HincII GTy'rAC
Cuts at: 804 1647 1850 804
Size: 843 203 3765
Fragments arranged by size:
3765 843 203
KasI G'GCGC_C
Cuts at: 1141 2241 2489 1141
Size: 1100 248 3463
Fragments arranged by size:
3463 1100 248
KpnI G_GTAC'C
Cuts at: 423 1356 1831 423
Size: 933 475 3403
Fragments arranged by size:
3403 933 475
MluI A'CGCG_T
Cuts at: 4244 4244
Size: 4811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1043 3482 3666 1043
Size: 2439 184 2188
Fragments arranged by size:
2439 2188 184
MscI TGG'CCA
Cuts at: 1561 1621 2572 1561
Size: 60 951 3800
Fragments arranged by size:
3800 951 60
MslI CAynn'nnrTG
Cuts at: 862 1327 1637 2800 2927 862
Size: 465 310 1163 127 2746
Fragments arranged by size:
2746 1163 465 310 127
MspA1I CmG'CkG
Cuts at: 301 330 1059 1565 1922 2236 2596 3609 3854
Size: 29 729 506 357 314 360 1013 245
Cuts at: 3854 301
Size: 1258
Fragments arranged by size:
1258 1013 729 506 360 357 314 245
29
NaeI GCC'GGC
Cuts at: 2992 2992
Size: 4811
NarI GG'CG_CC
Cuts at: 1142 2242 2490 1142
Size: 1100 248 3463
Fragments arranged by size:
3463 1100 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4811
NgoMIV G'CCGG_C
Cuts at: 2990 2990
Size: 4811
NspI r_CATG'y
Cuts at: 507 1074 1864 2364 3271 507
Size: 567 790 500 907 2047
Fragments arranged by size:
2047 907 790 567 500
PasI CC'CwG_GG
Cuts at: 1104 1104
Size: 4811
PciI A'CATG_T
Cuts at: 3267 3267
Size: 4811
PflMI CCAn_nnn'nTGG
Cuts at: 311 786 311
Size: 475 4336
PfoI T'CCnGG_A
Cuts at: 779 1535 779
Size: 756 4055
PmeI GTTT'AAAC
Cuts at: 1810 1810
Size: 4811
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 3950
Size: 32 4779
PpuMI rG'GwC_Cy
Cuts at: 390 390
Size: 4811
PspOMI G'GGCC_C
Cuts at: 171 1243 171
Size: 1072 3739
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 307 339 307
Size: 32 4779
PstI C_TGCA'G
Cuts at: 880 1631 1858 2543 880
Size: 751 227 685 3148
Fragments arranged by size:
3148 751 685 227
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4811
PvuII CAG'CTG
Cuts at: 1059 2236 2596 1059
Size: 1177 360 3274
Fragments arranged by size:
3274 1177 360
RsrII CG'GwC_CG
Cuts at: 3006 3006
Size: 4811
SacI G_AGCT'C
Cuts at: 1825 1825
Size: 4811
SalI G'TCGA_C
Cuts at: 1848 1848
Size: 4811
SapI GCTCTTCn'nnn_
Cuts at: 2834 3044 2834
Size: 210 4601
SbfI CC_TGCA'GG
Cuts at: 1858 1858
Size: 4811
ScaI AGT'ACT
Cuts at: 4328 4328
Size: 4811
SexAI A'CCwGG_T
Cuts at: 269 386 269
Size: 117 4694
SfcI C'TryA_G
Cuts at: 33 876 1627 1854 2539 3532 3723 33
Size: 843 751 227 685 993 191 1121
Fragments arranged by size:
1121 993 843 751 685 227 191
SfoI GGC'GCC
Cuts at: 1143 2243 2491 1143
Size: 1100 248 3463
Fragments arranged by size:
3463 1100 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4811
SmaI CCC'GGG
Cuts at: 1833 1833
Size: 4811
SmlI C'TyrA_G
Cuts at: 1456 1966 2881 3373 3635 1456
Size: 510 915 492 262 2632
Fragments arranged by size:
2632 915 510 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4811
SphI G_CATG'C
Cuts at: 1074 1864 2364 1074
Size: 790 500 3521
Fragments arranged by size:
3521 790 500
StyI C'CwwG_G
Cuts at: 107 440 1001 1082 1945 2299 107
Size: 333 561 81 863 354 2619
Fragments arranged by size:
2619 863 561 354 333 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 311 544 1243 2394 2527 2986 3195 311
Size: 233 699 1151 133 459 209 1927
Fragments arranged by size:
1927 1151 699 459 233 209 133
TatI w'GTAC_w
Cuts at: 4326 4326
Size: 4811
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4328 4360 4328
Size: 32 4779
Tth111I GACn'n_nGTC
Cuts at: 388 1297 2608 388
Size: 909 1311 2591
Fragments arranged by size:
2591 1311 909
XbaI T'CTAG_A
Cuts at: 1842 1842
Size: 4811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1389 2306 1389
Size: 917 3894
XmaI C'CCGG_G
Cuts at: 1831 1831
Size: 4811
ZraI GAC'GTC
Cuts at: 1542 1542
Size: 4811
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BfrBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI