Restriction map for
pF1KB8132
(Circular) (Six-base) MAPSORT of: pF1KB8132.seq Check: 151 from: 1 to: 4643
pF1KB8132 4643 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 275 1681 275
Size: 1406 3237
Acc65I G'GTAC_C
Cuts at: 1659 1659
Size: 4643
AclI AA'CG_TT
Cuts at: 4483 4483
Size: 4643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 844 1446 2468 2900 3647 3915 775
Size: 69 602 1022 432 747 268 1503
Fragments arranged by size:
1503 1022 747 602 432 268 69
AfeI AGC'GCT
Cuts at: 969 1426 969
Size: 457 4186
AflIII A'CryG_T
Cuts at: 1995 3099 4076 1995
Size: 1104 977 2562
Fragments arranged by size:
2562 1104 977
AgeI A'CCGG_T
Cuts at: 3031 3031
Size: 4643
AhdI GACnn_n'nnGTC
Cuts at: 1131 1131
Size: 4643
AlwNI CAG_nnn'CTG
Cuts at: 306 339 372 399 753 783 1948 3515 306
Size: 33 33 27 354 30 1165 1567 1434
Fragments arranged by size:
1567 1434 1165 354 33 33 30 27
ApaI G_GGCC'C
Cuts at: 296 1306 1537 296
Size: 1010 231 3402
Fragments arranged by size:
3402 1010 231
ApaLI G'TGCA_C
Cuts at: 3413 3413
Size: 4643
ApoI r'AATT_y
Cuts at: 268 1647 3996 4252 268
Size: 1379 2349 256 659
Fragments arranged by size:
2349 1379 659 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4643
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
AvaI C'yCGr_G
Cuts at: 208 1228 1252 1529 1663 208
Size: 1020 24 277 134 3188
Fragments arranged by size:
3188 1020 277 134 24
BamHI G'GATC_C
Cuts at: 1668 1668
Size: 4643
BanI G'GyrC_C
Cuts at: 645 1029 1066 1525 1659 2073 2321 2356 645
Size: 384 37 459 134 414 248 35 2932
Fragments arranged by size:
2932 459 414 384 248 134 37 35
BanII G_rGCy'C
Cuts at: 148 296 526 538 605 613 1306 1537 1657
Size: 148 230 12 67 8 693 231 120
Cuts at: 1657 2687 148
Size: 1030 2104
Fragments arranged by size:
2104 1030 693 231 230 148 120 67
12 8
BbeI G_GCGC'C
Cuts at: 1033 1529 2077 2325 1033
Size: 496 548 248 3351
Fragments arranged by size:
3351 548 496 248
BbsI GAAGACnn'nnnn_
Cuts at: 1456 1495 1456
Size: 39 4604
BbvCI CC'TCA_GC
Cuts at: 776 776
Size: 4643
BciVI GTATCCnnnnn_n'
Cuts at: 2541 3302 4643 2541
Size: 761 1341 2541
Fragments arranged by size:
2541 1341 761
BclI T'GATC_A
Cuts at: 2163 2163
Size: 4643
BglI GCCn_nnn'nGGC
Cuts at: 1256 1415 1256
Size: 159 4484
BglII A'GATC_T
Cuts at: 2158 4195 2158
Size: 2037 2606
BlpI GC'TnA_GC
Cuts at: 1755 1755
Size: 4643
Bme1580I G_kGCm'C
Cuts at: 296 1069 1198 1306 1537 2268 2361 3417 296
Size: 773 129 108 231 731 93 1056 1522
Fragments arranged by size:
1522 1056 773 731 231 129 108 93
BmrI ACTGGGnnnn_n'
Cuts at: 1216 1855 1936 2271 4415 1216
Size: 639 81 335 2144 1444
Fragments arranged by size:
2144 1444 639 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 372 1155 1278 3010 372
Size: 783 123 1732 2005
Fragments arranged by size:
2005 1732 783 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 394 409 867 1309 262
Size: 132 15 458 442 3596
Fragments arranged by size:
3596 458 442 132 15
Bpu10I CC'TnA_GC
Cuts at: 776 1013 776
Size: 237 4406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1819 2698 3190 3488 1819
Size: 879 492 298 2974
Fragments arranged by size:
2974 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 205 4347 205
Size: 4142 501
BsaAI yAC'GTr
Cuts at: 1996 1996
Size: 4643
BsaBI GATnn'nnATC
Cuts at: 6 2157 4194 6
Size: 2151 2037 455
Fragments arranged by size:
2151 2037 455
BsaHI Gr'CG_yC
Cuts at: 1030 1043 1259 1526 2074 2322 3024 4520 1030
Size: 13 216 267 548 248 702 1496 1153
Fragments arranged by size:
1496 1153 702 548 267 248 216 13
BsaWI w'CCGG_w
Cuts at: 672 833 905 1147 1832 2353 3031 3305 3452
Size: 161 72 242 685 521 678 274 147
Cuts at: 3452 3863 672
Size: 411 1452
Fragments arranged by size:
1452 685 678 521 411 274 242 161
147 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 865 895 112
Size: 30 723 30 3860
Fragments arranged by size:
3860 723 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 711 829 1000 1090 1093 711
Size: 118 171 90 3 4261
Fragments arranged by size:
4261 171 118 90 3
BseYI C'CCAG_C
Cuts at: 150 519 613 631 866 970 1518 2068 2826
Size: 369 94 18 235 104 548 550 758
Cuts at: 2826 3403 3899 150
Size: 577 496 894
Fragments arranged by size:
894 758 577 550 548 496 369 235
104 94 18
BsiEI CG_ry'CG
Cuts at: 104 1419 2185 2231 3439 104
Size: 1315 766 46 1208 1308
Fragments arranged by size:
1315 1308 1208 766 46
BsiHKAI G_wGCw'C
Cuts at: 148 605 1400 1484 1657 2435 2625 3417 148
Size: 457 795 84 173 778 190 792 1374
Fragments arranged by size:
1374 795 792 778 457 190 173 84
BsmBI CGTCTCn'nnnn_
Cuts at: 1266 1266
Size: 4643
Bsp1286I G_dGCh'C
Cuts at: 148 296 526 538 605 613 1069 1198 1306
Size: 148 230 12 67 8 456 129 108
Cuts at: 1306 1400 1484 1537 1657 2268 2361 2435 2625
Size: 94 84 53 120 611 93 74 190
Cuts at: 2625 2687 3417 148
Size: 62 730 1374
Fragments arranged by size:
1374 730 611 456 230 190 148 129
120 108 94 93 84 74 67 62
53 12 8
BspEI T'CCGG_A
Cuts at: 672 3863 672
Size: 3191 1452
BspHI T'CATG_A
Cuts at: 3819 3819
Size: 4643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1693 1907 2209 2590 1693
Size: 214 302 381 3746
Fragments arranged by size:
3746 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2935 2989 4473 4638 50
Size: 2885 54 1484 165 55
Fragments arranged by size:
2885 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2555 2555
Size: 4643
BsrFI r'CCGG_y
Cuts at: 2641 2822 3031 2641
Size: 181 209 4253
Fragments arranged by size:
4253 209 181
BssHII G'CGCG_C
Cuts at: 352 352
Size: 4643
BssSI C'ACGA_G
Cuts at: 1495 2914 3272 1495
Size: 1419 358 2866
Fragments arranged by size:
2866 1419 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2020 2020
Size: 4643
BstBI TT'CG_AA
Cuts at: 3004 3004
Size: 4643
BstXI CCAn_nnnn'nTGG
Cuts at: 625 625
Size: 4643
BstYI r'GATC_y
Cuts at: 1 162 638 921 1668 2135 2158 2493 2739
Size: 161 476 283 747 467 23 335 246
Cuts at: 2739 3751 3837 3849 4195 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 747 476 467 449 346 335 283
246 161 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 347 347
Size: 4643
BtgI C'CryG_G
Cuts at: 998 1332 1410 998
Size: 334 78 4231
Fragments arranged by size:
4231 334 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 569 2593 2774 2937 569
Size: 2024 181 163 2275
Fragments arranged by size:
2275 2024 181 163
BtsI GCAGTG_nn'
Cuts at: 1886 1886
Size: 4643
DraI TTT'AAA
Cuts at: 1642 1642
Size: 4643
DrdI GACnn_nn'nnGTC
Cuts at: 2349 3207 2349
Size: 858 3785
EaeI y'GGCC_r
Cuts at: 1416 2228 2402 2793 2820 4165 1416
Size: 812 174 391 27 1345 1894
Fragments arranged by size:
1894 1345 812 391 174 27
EagI C'GGCC_G
Cuts at: 1416 2228 1416
Size: 812 3831
EarI CTCTTCn'nnn_
Cuts at: 497 716 820 1079 2666 2876 497
Size: 219 104 259 1587 210 2264
Fragments arranged by size:
2264 1587 259 219 210 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1595 2851 3161 3307 4453 1595
Size: 1256 310 146 1146 1785
Fragments arranged by size:
1785 1256 1146 310 146
EcoICRI GAG'CTC
Cuts at: 146 603 1655 146
Size: 457 1052 3134
Fragments arranged by size:
3134 1052 457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 394 409 775 844 867 1309 1446 2468
Size: 132 15 366 69 23 442 137 1022
Cuts at: 2468 2900 3647 3915 262
Size: 432 747 268 990
Fragments arranged by size:
1022 990 747 442 432 366 268 137
132 69 23 15
EcoNI CCTnn'n_nnAGG
Cuts at: 138 478 138
Size: 340 4303
EcoO109I rG'GnC_Cy
Cuts at: 227 293 483 621 771 1101 1116 1302 1303
Size: 66 190 138 150 330 15 186 1
Cuts at: 1303 1533 1534 1782 227
Size: 230 1 248 3088
Fragments arranged by size:
3088 330 248 230 190 186 150 138
66 15 1 1
EcoRI G'AATT_C
Cuts at: 1647 1647
Size: 4643
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4643
FspI TGC'GCA
Cuts at: 2424 2424
Size: 4643
HaeII r_GCGC'y
Cuts at: 971 1033 1428 1529 2077 2325 3347 4326 971
Size: 62 395 101 548 248 1022 979 1288
Fragments arranged by size:
1288 1022 979 548 395 248 101 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 865 897 1045 1077 1117 1149 2485 2515 2517
Size: 32 148 32 40 32 1336 30 2
Cuts at: 2517 2547 865
Size: 30 2961
Fragments arranged by size:
2961 1336 148 40 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1682 1682
Size: 4643
KasI G'GCGC_C
Cuts at: 1029 1525 2073 2321 1029
Size: 496 548 248 3351
Fragments arranged by size:
3351 548 496 248
KpnI G_GTAC'C
Cuts at: 1663 1663
Size: 4643
MluI A'CGCG_T
Cuts at: 4076 4076
Size: 4643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1160 3314 3498 1160
Size: 2154 184 2305
Fragments arranged by size:
2305 2154 184
MscI TGG'CCA
Cuts at: 2404 2404
Size: 4643
MslI CAynn'nnrTG
Cuts at: 2632 2759 2632
Size: 127 4516
MspA1I CmG'CkG
Cuts at: 1000 1334 1754 2068 2428 3441 3686 1000
Size: 334 420 314 360 1013 245 1957
Fragments arranged by size:
1957 1013 420 360 334 314 245
NaeI GCC'GGC
Cuts at: 2824 2824
Size: 4643
NarI GG'CG_CC
Cuts at: 1030 1526 2074 2322 1030
Size: 496 548 248 3351
Fragments arranged by size:
3351 548 496 248
NcoI C'CATG_G
Cuts at: 1410 1410
Size: 4643
NgoMIV G'CCGG_C
Cuts at: 2822 2822
Size: 4643
NruI TCG'CGA
Cuts at: 607 607
Size: 4643
NspI r_CATG'y
Cuts at: 1696 2196 3103 1696
Size: 500 907 3236
Fragments arranged by size:
3236 907 500
PasI CC'CwG_GG
Cuts at: 428 1036 1306 428
Size: 608 270 3765
Fragments arranged by size:
3765 608 270
PciI A'CATG_T
Cuts at: 3099 3099
Size: 4643
PflMI CCAn_nnn'nTGG
Cuts at: 306 306
Size: 4643
PfoI T'CCnGG_A
Cuts at: 263 497 263
Size: 234 4409
PmeI GTTT'AAAC
Cuts at: 1642 1642
Size: 4643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 3782
Size: 32 4611
PpuMI rG'GwC_Cy
Cuts at: 227 771 1116 227
Size: 544 345 3754
Fragments arranged by size:
3754 544 345
PspOMI G'GGCC_C
Cuts at: 292 1302 1533 292
Size: 1010 231 3402
Fragments arranged by size:
3402 1010 231
PspXI vC'TCGA_Gb
Cuts at: 1252 1252
Size: 4643
PstI C_TGCA'G
Cuts at: 1690 2375 1690
Size: 685 3958
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4643
PvuII CAG'CTG
Cuts at: 2068 2428 2068
Size: 360 4283
RsrII CG'GwC_CG
Cuts at: 2838 2838
Size: 4643
SacI G_AGCT'C
Cuts at: 148 605 1657 148
Size: 457 1052 3134
Fragments arranged by size:
3134 1052 457
SacII CC_GC'GG
Cuts at: 1001 1335 1001
Size: 334 4309
SalI G'TCGA_C
Cuts at: 1680 1680
Size: 4643
SapI GCTCTTCn'nnn_
Cuts at: 497 820 1079 2666 2876 497
Size: 323 259 1587 210 2264
Fragments arranged by size:
2264 1587 323 259 210
SbfI CC_TGCA'GG
Cuts at: 1690 1690
Size: 4643
ScaI AGT'ACT
Cuts at: 4160 4160
Size: 4643
SfcI C'TryA_G
Cuts at: 33 1686 2371 3364 3555 33
Size: 1653 685 993 191 1121
Fragments arranged by size:
1653 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1031 1527 2075 2323 1031
Size: 496 548 248 3351
Fragments arranged by size:
3351 548 496 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
SmaI CCC'GGG
Cuts at: 1531 1665 1531
Size: 134 4509
SmlI C'TyrA_G
Cuts at: 1252 1798 2713 3205 3467 1252
Size: 546 915 492 262 2428
Fragments arranged by size:
2428 915 546 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4643
SphI G_CATG'C
Cuts at: 1696 2196 1696
Size: 500 4143
StuI AGG'CCT
Cuts at: 1369 1369
Size: 4643
StyI C'CwwG_G
Cuts at: 359 624 1410 1777 2131 359
Size: 265 786 367 354 2871
Fragments arranged by size:
2871 786 367 354 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 420 733 962 2226 2359 2818 3027 420
Size: 313 229 1264 133 459 209 2036
Fragments arranged by size:
2036 1264 459 313 229 209 133
TatI w'GTAC_w
Cuts at: 4158 4158
Size: 4643
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4160 4192 4160
Size: 32 4611
Tth111I GACn'n_nGTC
Cuts at: 2440 2440
Size: 4643
XbaI T'CTAG_A
Cuts at: 1674 1674
Size: 4643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1408 2138 114
Size: 1294 730 2619
Fragments arranged by size:
2619 1294 730
XhoI C'TCGA_G
Cuts at: 1252 1252
Size: 4643
XmaI C'CCGG_G
Cuts at: 1529 1663 1529
Size: 134 4509
XmnI GAAnn'nnTTC
Cuts at: 1453 1453
Size: 4643
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmI
BsrGI BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PmlI PshAI PsiI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI