Restriction map for
pF1KB8127
(Circular) (Six-base) MAPSORT of: pF1KB8127.seq Check: 2854 from: 1 to: 4466
pF1KB8127 4466 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 257 257
Size: 4466
AccI GT'mk_AC
Cuts at: 1504 1504
Size: 4466
Acc65I G'GTAC_C
Cuts at: 587 1482 587
Size: 895 3571
AclI AA'CG_TT
Cuts at: 4306 4306
Size: 4466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2723 3470 3738 2291
Size: 432 747 268 3019
Fragments arranged by size:
3019 747 432 268
AflIII A'CryG_T
Cuts at: 1343 1818 2922 3899 1343
Size: 475 1104 977 1910
Fragments arranged by size:
1910 1104 977 475
AgeI A'CCGG_T
Cuts at: 2854 2854
Size: 4466
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1330 1362 1330
Size: 32 4434
AlwNI CAG_nnn'CTG
Cuts at: 1771 3338 1771
Size: 1567 2899
ApaI G_GGCC'C
Cuts at: 1165 1165
Size: 4466
ApaLI G'TGCA_C
Cuts at: 3236 3236
Size: 4466
ApoI r'AATT_y
Cuts at: 446 1470 3819 4075 446
Size: 1024 2349 256 837
Fragments arranged by size:
2349 1024 837 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4466
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
AvaI C'yCGr_G
Cuts at: 113 205 293 320 1321 1486 113
Size: 92 88 27 1001 165 3093
Fragments arranged by size:
3093 1001 165 92 88 27
BamHI G'GATC_C
Cuts at: 1124 1491 1124
Size: 367 4099
BanI G'GyrC_C
Cuts at: 587 1447 1482 1896 2144 2179 587
Size: 860 35 414 248 35 2874
Fragments arranged by size:
2874 860 414 248 35 35
BanII G_rGCy'C
Cuts at: 559 1165 1235 1400 1480 2510 559
Size: 606 70 165 80 1030 2515
Fragments arranged by size:
2515 1030 606 165 80 70
BbeI G_GCGC'C
Cuts at: 1900 2148 1900
Size: 248 4218
BbsI GAAGACnn'nnnn_
Cuts at: 461 973 461
Size: 512 3954
BbvCI CC'TCA_GC
Cuts at: 1212 1212
Size: 4466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4432
BciVI GTATCCnnnnn_n'
Cuts at: 2364 3125 4466 2364
Size: 761 1341 2364
Fragments arranged by size:
2364 1341 761
BclI T'GATC_A
Cuts at: 1986 1986
Size: 4466
BglI GCCn_nnn'nGGC
Cuts at: 146 209 146
Size: 63 4403
BglII A'GATC_T
Cuts at: 1981 4018 1981
Size: 2037 2429
BlpI GC'TnA_GC
Cuts at: 1578 1578
Size: 4466
Bme1580I G_kGCm'C
Cuts at: 241 1165 1450 2091 2184 3240 241
Size: 924 285 641 93 1056 1467
Fragments arranged by size:
1467 1056 924 641 285 93
BmgBI CAC'GTC
Cuts at: 1344 1344
Size: 4466
BmrI ACTGGGnnnn_n'
Cuts at: 426 1678 1759 2094 4238 426
Size: 1252 81 335 2144 654
Fragments arranged by size:
2144 1252 654 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1214 2833 1214
Size: 1619 2847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 589
Size: 4466
Bpu10I CC'TnA_GC
Cuts at: 1212 1212
Size: 4466
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1642 2521 3013 3311 667
Size: 975 879 492 298 1822
Fragments arranged by size:
1822 975 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1028 4170 1028
Size: 3142 1324
BsaAI yAC'GTr
Cuts at: 1819 1819
Size: 4466
BsaBI GATnn'nnATC
Cuts at: 6 1980 4017 6
Size: 1974 2037 455
Fragments arranged by size:
2037 1974 455
BsaHI Gr'CG_yC
Cuts at: 254 1897 2145 2847 4343 254
Size: 1643 248 702 1496 377
Fragments arranged by size:
1643 1496 702 377 248
BsaWI w'CCGG_w
Cuts at: 740 1655 2176 2854 3128 3275 3686 740
Size: 915 521 678 274 147 411 1520
Fragments arranged by size:
1520 915 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1330 1360 1330
Size: 30 4436
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 413 416 1251 235
Size: 178 3 835 3450
Fragments arranged by size:
3450 835 178 3
BseYI C'CCAG_C
Cuts at: 135 532 1424 1891 2649 3226 3722 135
Size: 397 892 467 758 577 496 879
Fragments arranged by size:
892 879 758 577 496 467 397
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 745 625
Size: 120 4346
BsiEI CG_ry'CG
Cuts at: 104 2008 2054 3262 104
Size: 1904 46 1208 1308
Fragments arranged by size:
1904 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 284 1400 1480 2258 2448 3240 284
Size: 1116 80 778 190 792 1510
Fragments arranged by size:
1510 1116 792 778 190 80
Bsp1286I G_dGCh'C
Cuts at: 241 284 559 1165 1235 1400 1450 1480 2091
Size: 43 275 606 70 165 50 30 611
Cuts at: 2091 2184 2258 2448 2510 3240 241
Size: 93 74 190 62 730 1467
Fragments arranged by size:
1467 730 611 606 275 190 165 93
74 70 62 50 43 30
BspEI T'CCGG_A
Cuts at: 3686 3686
Size: 4466
BspHI T'CATG_A
Cuts at: 450 914 986 1235 3642 450
Size: 464 72 249 2407 1274
Fragments arranged by size:
2407 1274 464 249 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1214 1449 1516 1730 2032 2413 1214
Size: 235 67 214 302 381 3267
Fragments arranged by size:
3267 381 302 235 214 67
BsrBI CCG'CTC
Cuts at: 50 933 2758 2812 4296 4461 50
Size: 883 1825 54 1484 165 55
Fragments arranged by size:
1825 1484 883 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2378 2378
Size: 4466
BsrFI r'CCGG_y
Cuts at: 919 2464 2645 2854 919
Size: 1545 181 209 2531
Fragments arranged by size:
2531 1545 209 181
BssSI C'ACGA_G
Cuts at: 2737 3095 2737
Size: 358 4108
BstAPI GCAn_nnn'nTGC
Cuts at: 1843 1843
Size: 4466
BstBI TT'CG_AA
Cuts at: 2827 2827
Size: 4466
BstXI CCAn_nnnn'nTGG
Cuts at: 978 978
Size: 4466
BstYI r'GATC_y
Cuts at: 1 1124 1491 1958 1981 2316 2562 3574 3660
Size: 1123 367 467 23 335 246 1012 86
Cuts at: 3660 3672 4018 1
Size: 12 346 449
Fragments arranged by size:
1123 1012 467 449 367 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 898 971 1156 898
Size: 73 185 4208
Fragments arranged by size:
4208 185 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 513 861 1044 2416 2597 2760 513
Size: 348 183 1372 181 163 2219
Fragments arranged by size:
2219 1372 348 183 181 163
BtsI GCAGTG_nn'
Cuts at: 1050 1709 1050
Size: 659 3807
DraI TTT'AAA
Cuts at: 1465 1465
Size: 4466
DrdI GACnn_nn'nnGTC
Cuts at: 2172 3030 2172
Size: 858 3608
EaeI y'GGCC_r
Cuts at: 818 953 1142 1241 1418 2051 2225 2616 2643
Size: 135 189 99 177 633 174 391 27
Cuts at: 2643 3988 818
Size: 1345 1296
Fragments arranged by size:
1345 1296 633 391 189 177 174 135
99 27
EagI C'GGCC_G
Cuts at: 2051 2051
Size: 4466
EarI CTCTTCn'nnn_
Cuts at: 2489 2699 2489
Size: 210 4256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2674 2984 3130 4276 2674
Size: 310 146 1146 2864
Fragments arranged by size:
2864 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1398 1478 1398
Size: 80 4386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 2291 2723 3470 3738 589
Size: 1702 432 747 268 1317
Fragments arranged by size:
1702 1317 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1161 1162 1605 1161
Size: 1 443 4022
Fragments arranged by size:
4022 443 1
EcoRI G'AATT_C
Cuts at: 1470 1470
Size: 4466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 273 1374 2485 273
Size: 1101 1111 2254
Fragments arranged by size:
2254 1111 1101
FspI TGC'GCA
Cuts at: 2247 2247
Size: 4466
HaeII r_GCGC'y
Cuts at: 1900 2148 3170 4149 1900
Size: 248 1022 979 2217
Fragments arranged by size:
2217 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2308 2338 2340 2370 2308
Size: 30 2 30 4404
Fragments arranged by size:
4404 30 30 2
HincII GTy'rAC
Cuts at: 1505 1505
Size: 4466
KasI G'GCGC_C
Cuts at: 1896 2144 1896
Size: 248 4218
KpnI G_GTAC'C
Cuts at: 591 1486 591
Size: 895 3571
MluI A'CGCG_T
Cuts at: 3899 3899
Size: 4466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 368 3137 3321 368
Size: 2769 184 1513
Fragments arranged by size:
2769 1513 184
MscI TGG'CCA
Cuts at: 955 1144 1243 2227 955
Size: 189 99 984 3194
Fragments arranged by size:
3194 984 189 99
MslI CAynn'nnrTG
Cuts at: 811 1177 2455 2582 811
Size: 366 1278 127 2695
Fragments arranged by size:
2695 1278 366 127
MspA1I CmG'CkG
Cuts at: 135 180 425 798 1229 1577 1891 2251 3264
Size: 45 245 373 431 348 314 360 1013
Cuts at: 3264 3509 135
Size: 245 1092
Fragments arranged by size:
1092 1013 431 373 360 348 314 245
245 45
NaeI GCC'GGC
Cuts at: 2647 2647
Size: 4466
NarI GG'CG_CC
Cuts at: 1897 2145 1897
Size: 248 4218
NgoMIV G'CCGG_C
Cuts at: 2645 2645
Size: 4466
NspI r_CATG'y
Cuts at: 1519 2019 2926 1519
Size: 500 907 3059
Fragments arranged by size:
3059 907 500
PasI CC'CwG_GG
Cuts at: 435 435
Size: 4466
PciI A'CATG_T
Cuts at: 2922 2922
Size: 4466
PflMI CCAn_nnn'nTGG
Cuts at: 951 951
Size: 4466
PfoI T'CCnGG_A
Cuts at: 566 977 1127 566
Size: 411 150 3905
Fragments arranged by size:
3905 411 150
PmeI GTTT'AAAC
Cuts at: 1465 1465
Size: 4466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1330 1362 3605 3637 1330
Size: 32 2243 32 2159
Fragments arranged by size:
2243 2159 32 32
PshAI GACnn'nnGTC
Cuts at: 973 973
Size: 4466
PspOMI G'GGCC_C
Cuts at: 1161 1161
Size: 4466
PspXI vC'TCGA_Gb
Cuts at: 1321 1321
Size: 4466
PstI C_TGCA'G
Cuts at: 134 1004 1121 1513 2198 134
Size: 870 117 392 685 2402
Fragments arranged by size:
2402 870 685 392 117
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4466
PvuII CAG'CTG
Cuts at: 135 180 798 1891 2251 135
Size: 45 618 1093 360 2350
Fragments arranged by size:
2350 1093 618 360 45
RsrII CG'GwC_CG
Cuts at: 2661 2661
Size: 4466
SacI G_AGCT'C
Cuts at: 1400 1480 1400
Size: 80 4386
SalI G'TCGA_C
Cuts at: 1503 1503
Size: 4466
SapI GCTCTTCn'nnn_
Cuts at: 2489 2699 2489
Size: 210 4256
SbfI CC_TGCA'GG
Cuts at: 1513 1513
Size: 4466
ScaI AGT'ACT
Cuts at: 3983 3983
Size: 4466
SfcI C'TryA_G
Cuts at: 33 130 938 1000 1117 1509 2194 3187 3378
Size: 97 808 62 117 392 685 993 191
Cuts at: 3378 33
Size: 1121
Fragments arranged by size:
1121 993 808 685 392 191 117 97
62
SfoI GGC'GCC
Cuts at: 1898 2146 1898
Size: 248 4218
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
SmaI CCC'GGG
Cuts at: 115 295 322 1488 115
Size: 180 27 1166 3093
Fragments arranged by size:
3093 1166 180 27
SmlI C'TyrA_G
Cuts at: 682 1321 1621 2536 3028 3290 682
Size: 639 300 915 492 262 1858
Fragments arranged by size:
1858 915 639 492 300 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4466
SphI G_CATG'C
Cuts at: 1519 2019 1519
Size: 500 3966
StyI C'CwwG_G
Cuts at: 1600 1954 1600
Size: 354 4112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 491 1114 1438 1465 2049 2182 2641 2850 491
Size: 623 324 27 584 133 459 209 2107
Fragments arranged by size:
2107 623 584 459 324 209 133 27
TatI w'GTAC_w
Cuts at: 3981 3981
Size: 4466
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3983 4015 3983
Size: 32 4434
Tth111I GACn'n_nGTC
Cuts at: 2263 2263
Size: 4466
XbaI T'CTAG_A
Cuts at: 1497 1497
Size: 4466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1014 1176 1961 1014
Size: 162 785 3519
Fragments arranged by size:
3519 785 162
XhoI C'TCGA_G
Cuts at: 1321 1321
Size: 4466
XmaI C'CCGG_G
Cuts at: 113 293 320 1486 113
Size: 180 27 1166 3093
Fragments arranged by size:
3093 1166 180 27
ZraI GAC'GTC
Cuts at: 255 255
Size: 4466
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PmlI PpuMI PsiI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI