Restriction map for
pF1KB8125
(Circular) (Six-base) MAPSORT of: pF1KB8125.seq Check: 5989 from: 1 to: 4478
pF1KB8125 4478 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 740 740
Size: 4478
AccI GT'mk_AC
Cuts at: 675 1364 1516 675
Size: 689 152 3637
Fragments arranged by size:
3637 689 152
Acc65I G'GTAC_C
Cuts at: 1494 1494
Size: 4478
AclI AA'CG_TT
Cuts at: 4318 4318
Size: 4478
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 1418 2303 2735 3482 3750 250
Size: 1168 885 432 747 268 978
Fragments arranged by size:
1168 978 885 747 432 268
AflII C'TTAA_G
Cuts at: 972 972
Size: 4478
AflIII A'CryG_T
Cuts at: 876 1830 2934 3911 876
Size: 954 1104 977 1443
Fragments arranged by size:
1443 1104 977 954
AgeI A'CCGG_T
Cuts at: 2866 2866
Size: 4478
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1418 1450 1418
Size: 32 4446
AlwNI CAG_nnn'CTG
Cuts at: 159 1783 3350 159
Size: 1624 1567 1287
Fragments arranged by size:
1624 1567 1287
ApaLI G'TGCA_C
Cuts at: 3248 3248
Size: 4478
ApoI r'AATT_y
Cuts at: 126 162 388 667 1293 1482 3831 4087 126
Size: 36 226 279 626 189 2349 256 517
Fragments arranged by size:
2349 626 517 279 256 226 189 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 4478
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4478
AvaI C'yCGr_G
Cuts at: 117 1498 117
Size: 1381 3097
AvrII C'CTAG_G
Cuts at: 928 928
Size: 4478
BamHI G'GATC_C
Cuts at: 1503 1503
Size: 4478
BanI G'GyrC_C
Cuts at: 1494 1908 2156 2191 1494
Size: 414 248 35 3781
Fragments arranged by size:
3781 414 248 35
BanII G_rGCy'C
Cuts at: 316 1492 2522 316
Size: 1176 1030 2272
Fragments arranged by size:
2272 1176 1030
BbeI G_GCGC'C
Cuts at: 1912 2160 1912
Size: 248 4230
BbsI GAAGACnn'nnnn_
Cuts at: 1313 1313
Size: 4478
BbvCI CC'TCA_GC
Cuts at: 1469 1469
Size: 4478
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 749 783 749
Size: 34 4444
BciVI GTATCCnnnnn_n'
Cuts at: 2376 3137 4478 2376
Size: 761 1341 2376
Fragments arranged by size:
2376 1341 761
BclI T'GATC_A
Cuts at: 1257 1998 1257
Size: 741 3737
BglII A'GATC_T
Cuts at: 1993 4030 1993
Size: 2037 2441
BlpI GC'TnA_GC
Cuts at: 1590 1590
Size: 4478
Bme1580I G_kGCm'C
Cuts at: 281 2103 2196 3252 281
Size: 1822 93 1056 1507
Fragments arranged by size:
1822 1507 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 168 1690 1771 2106 4250 168
Size: 1522 81 335 2144 396
Fragments arranged by size:
2144 1522 396 335 81
BmtI G_CTAG'C
Cuts at: 1411 1411
Size: 4478
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1016 1368 2845 1016
Size: 352 1477 2649
Fragments arranged by size:
2649 1477 352
Bpu10I CC'TnA_GC
Cuts at: 315 1469 315
Size: 1154 3324
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 1654 2533 3025 3323 471
Size: 1183 879 492 298 1626
Fragments arranged by size:
1626 1183 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4182 4182
Size: 4478
BsaAI yAC'GTr
Cuts at: 1831 1831
Size: 4478
BsaBI GATnn'nnATC
Cuts at: 6 1992 4029 6
Size: 1986 2037 455
Fragments arranged by size:
2037 1986 455
BsaHI Gr'CG_yC
Cuts at: 737 1909 2157 2859 4355 737
Size: 1172 248 702 1496 860
Fragments arranged by size:
1496 1172 860 702 248
BsaWI w'CCGG_w
Cuts at: 1667 2188 2866 3140 3287 3698 1667
Size: 521 678 274 147 411 2447
Fragments arranged by size:
2447 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1055 1085 1055
Size: 30 4448
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 763 763
Size: 4478
BseYI C'CCAG_C
Cuts at: 935 1113 1903 2661 3238 3734 935
Size: 178 790 758 577 496 1679
Fragments arranged by size:
1679 790 758 577 496 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 526 265
Size: 261 4217
BsiEI CG_ry'CG
Cuts at: 104 2020 2066 3274 104
Size: 1916 46 1208 1308
Fragments arranged by size:
1916 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 316 1492 2270 2460 3252 316
Size: 1176 778 190 792 1542
Fragments arranged by size:
1542 1176 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 281 316 1492 2103 2196 2270 2460 2522 3252
Size: 35 1176 611 93 74 190 62 730
Cuts at: 3252 281
Size: 1507
Fragments arranged by size:
1507 1176 730 611 190 93 74 62
35
BspEI T'CCGG_A
Cuts at: 3698 3698
Size: 4478
BspHI T'CATG_A
Cuts at: 365 740 3654 365
Size: 375 2914 1189
Fragments arranged by size:
2914 1189 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 221 1528 1742 2044 2425 221
Size: 1307 214 302 381 2274
Fragments arranged by size:
2274 1307 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2770 2824 4308 4473 50
Size: 2720 54 1484 165 55
Fragments arranged by size:
2720 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2390 2390
Size: 4478
BsrFI r'CCGG_y
Cuts at: 2476 2657 2866 2476
Size: 181 209 4088
Fragments arranged by size:
4088 209 181
BssHII G'CGCG_C
Cuts at: 249 251 249
Size: 2 4476
BssSI C'ACGA_G
Cuts at: 2749 3107 2749
Size: 358 4120
BstAPI GCAn_nnn'nTGC
Cuts at: 276 513 1855 276
Size: 237 1342 2899
Fragments arranged by size:
2899 1342 237
BstBI TT'CG_AA
Cuts at: 2839 2839
Size: 4478
BstEII G'GTnAC_C
Cuts at: 1324 1324
Size: 4478
BstYI r'GATC_y
Cuts at: 1 329 1503 1970 1993 2328 2574 3586 3672
Size: 328 1174 467 23 335 246 1012 86
Cuts at: 3672 3684 4030 1
Size: 12 346 449
Fragments arranged by size:
1174 1012 467 449 346 335 328 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 676 676
Size: 4478
Bsu36I CC'TnA_GG
Cuts at: 488 488
Size: 4478
BtgI C'CryG_G
Cuts at: 239 1311 1454 239
Size: 1072 143 3263
Fragments arranged by size:
3263 1072 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 336 2428 2609 2772 336
Size: 2092 181 163 2042
Fragments arranged by size:
2092 2042 181 163
BtsI GCAGTG_nn'
Cuts at: 1235 1721 1235
Size: 486 3992
DraI TTT'AAA
Cuts at: 1477 1477
Size: 4478
DraIII CAC_nnn'GTG
Cuts at: 276 276
Size: 4478
DrdI GACnn_nn'nnGTC
Cuts at: 2184 3042 2184
Size: 858 3620
EaeI y'GGCC_r
Cuts at: 540 725 1314 2063 2237 2628 2655 4000 540
Size: 185 589 749 174 391 27 1345 1018
Fragments arranged by size:
1345 1018 749 589 391 185 174 27
EagI C'GGCC_G
Cuts at: 2063 2063
Size: 4478
EarI CTCTTCn'nnn_
Cuts at: 304 895 1394 2501 2711 304
Size: 591 499 1107 210 2071
Fragments arranged by size:
2071 1107 591 499 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2686 2996 3142 4288 2686
Size: 310 146 1146 2876
Fragments arranged by size:
2876 1146 310 146
EcoICRI GAG'CTC
Cuts at: 314 1490 314
Size: 1176 3302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 1418 2303 2735 3482 3750 250
Size: 1168 885 432 747 268 978
Fragments arranged by size:
1168 978 885 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 932 1449 1617 932
Size: 517 168 3793
Fragments arranged by size:
3793 517 168
EcoRI G'AATT_C
Cuts at: 126 162 667 1293 1482 126
Size: 36 505 626 189 3122
Fragments arranged by size:
3122 626 505 189 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 615 906 2497 615
Size: 291 1591 2596
Fragments arranged by size:
2596 1591 291
FspI TGC'GCA
Cuts at: 2259 2259
Size: 4478
HaeII r_GCGC'y
Cuts at: 1912 2160 3182 4161 1912
Size: 248 1022 979 2229
Fragments arranged by size:
2229 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 314 346 2320 2350 2352 2382 314
Size: 32 1974 30 2 30 2410
Fragments arranged by size:
2410 1974 32 30 30 2
HincII GTy'rAC
Cuts at: 1517 1517
Size: 4478
KasI G'GCGC_C
Cuts at: 1908 2156 1908
Size: 248 4230
KpnI G_GTAC'C
Cuts at: 1498 1498
Size: 4478
MluI A'CGCG_T
Cuts at: 3911 3911
Size: 4478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 191 966 1071 3149 3333 191
Size: 775 105 2078 184 1336
Fragments arranged by size:
2078 1336 775 184 105
MscI TGG'CCA
Cuts at: 542 1316 2239 542
Size: 774 923 2781
Fragments arranged by size:
2781 923 774
MslI CAynn'nnrTG
Cuts at: 2467 2594 2467
Size: 127 4351
MspA1I CmG'CkG
Cuts at: 1589 1903 2263 3276 3521 1589
Size: 314 360 1013 245 2546
Fragments arranged by size:
2546 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2659 2659
Size: 4478
NarI GG'CG_CC
Cuts at: 1909 2157 1909
Size: 248 4230
NcoI C'CATG_G
Cuts at: 1311 1454 1311
Size: 143 4335
NgoMIV G'CCGG_C
Cuts at: 2657 2657
Size: 4478
NheI G'CTAG_C
Cuts at: 1407 1407
Size: 4478
NspI r_CATG'y
Cuts at: 262 880 1393 1531 2031 2938 262
Size: 618 513 138 500 907 1802
Fragments arranged by size:
1802 907 618 513 500 138
PciI A'CATG_T
Cuts at: 876 2934 876
Size: 2058 2420
PmeI GTTT'AAAC
Cuts at: 1477 1477
Size: 4478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 3617
Size: 32 4446
PpuMI rG'GwC_Cy
Cuts at: 1449 1449
Size: 4478
PshAI GACnn'nnGTC
Cuts at: 224 224
Size: 4478
PstI C_TGCA'G
Cuts at: 1525 2210 1525
Size: 685 3793
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4478
PvuII CAG'CTG
Cuts at: 1903 2263 1903
Size: 360 4118
RsrII CG'GwC_CG
Cuts at: 2673 2673
Size: 4478
SacI G_AGCT'C
Cuts at: 316 1492 316
Size: 1176 3302
SalI G'TCGA_C
Cuts at: 1515 1515
Size: 4478
SanDI GG'GwC_CC
Cuts at: 1449 1449
Size: 4478
SapI GCTCTTCn'nnn_
Cuts at: 304 2501 2711 304
Size: 2197 210 2071
Fragments arranged by size:
2197 2071 210
SbfI CC_TGCA'GG
Cuts at: 1525 1525
Size: 4478
ScaI AGT'ACT
Cuts at: 3995 3995
Size: 4478
SexAI A'CCwGG_T
Cuts at: 689 689
Size: 4478
SfcI C'TryA_G
Cuts at: 33 1521 2206 3199 3390 33
Size: 1488 685 993 191 1121
Fragments arranged by size:
1488 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1910 2158 1910
Size: 248 4230
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4478
SmaI CCC'GGG
Cuts at: 1500 1500
Size: 4478
SmlI C'TyrA_G
Cuts at: 450 972 1633 2548 3040 3302 450
Size: 522 661 915 492 262 1626
Fragments arranged by size:
1626 915 661 522 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4478
SphI G_CATG'C
Cuts at: 1531 2031 1531
Size: 500 3978
SspI AAT'ATT
Cuts at: 381 564 381
Size: 183 4295
StyI C'CwwG_G
Cuts at: 857 928 1311 1454 1612 1966 857
Size: 71 383 143 158 354 3369
Fragments arranged by size:
3369 383 354 158 143 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 220 2061 2194 2653 2862 220
Size: 1841 133 459 209 1836
Fragments arranged by size:
1841 1836 459 209 133
TatI w'GTAC_w
Cuts at: 944 3993 944
Size: 3049 1429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1055 1087 3995 4027 1055
Size: 32 2908 32 1506
Fragments arranged by size:
2908 1506 32 32
Tth111I GACn'n_nGTC
Cuts at: 2275 2275
Size: 4478
XbaI T'CTAG_A
Cuts at: 1509 1509
Size: 4478
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 855 1461 1973 855
Size: 606 512 3360
Fragments arranged by size:
3360 606 512
XmaI C'CCGG_G
Cuts at: 1498 1498
Size: 4478
XmnI GAAnn'nnTTC
Cuts at: 598 598
Size: 4478
ZraI GAC'GTC
Cuts at: 738 738
Size: 4478
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PciI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AlfI ApaI AscI BaeI
BfrBI BglI BmgBI BpmI BsiWI BsmI BsmBI BsrGI
BstXI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PsiI PspOMI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI