Restriction map for
pF1KB8124
(Circular) (Six-base) MAPSORT of: pF1KB8124.seq Check: 5418 from: 1 to: 4310
pF1KB8124 4310 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 677 677
Size: 4310
AccI GT'mk_AC
Cuts at: 235 1348 235
Size: 1113 3197
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 4150 4150
Size: 4310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 830 2135 2567 3314 3582 667
Size: 163 1305 432 747 268 1395
Fragments arranged by size:
1395 1305 747 432 268 163
AflIII A'CryG_T
Cuts at: 1662 2766 3743 1662
Size: 1104 977 2229
Fragments arranged by size:
2229 1104 977
AgeI A'CCGG_T
Cuts at: 583 2698 583
Size: 2115 2195
AlwNI CAG_nnn'CTG
Cuts at: 645 1615 3182 645
Size: 970 1567 1773
Fragments arranged by size:
1773 1567 970
ApaI G_GGCC'C
Cuts at: 149 415 459 149
Size: 266 44 4000
Fragments arranged by size:
4000 266 44
ApaLI G'TGCA_C
Cuts at: 3080 3080
Size: 4310
ApoI r'AATT_y
Cuts at: 1258 1291 1314 3663 3919 1258
Size: 33 23 2349 256 1649
Fragments arranged by size:
2349 1649 256 33 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 283 307 330 451 881 1330 283
Size: 24 23 121 430 449 3263
Fragments arranged by size:
3263 449 430 121 24 23
BamHI G'GATC_C
Cuts at: 1335 1335
Size: 4310
BanI G'GyrC_C
Cuts at: 259 469 1326 1740 1988 2023 259
Size: 210 857 414 248 35 2546
Fragments arranged by size:
2546 857 414 248 210 35
BanII G_rGCy'C
Cuts at: 149 284 415 459 619 718 916 1324 2354
Size: 135 131 44 160 99 198 408 1030
Cuts at: 2354 149
Size: 2105
Fragments arranged by size:
2105 1030 408 198 160 135 131 99
44
BbeI G_GCGC'C
Cuts at: 473 1744 1992 473
Size: 1271 248 2791
Fragments arranged by size:
2791 1271 248
BbsI GAAGACnn'nnnn_
Cuts at: 980 980
Size: 4310
BciVI GTATCCnnnnn_n'
Cuts at: 1227 2208 2969 4310 1227
Size: 981 761 1341 1227
Fragments arranged by size:
1341 1227 981 761
BclI T'GATC_A
Cuts at: 1830 1830
Size: 4310
BglI GCCn_nnn'nGGC
Cuts at: 424 492 424
Size: 68 4242
BglII A'GATC_T
Cuts at: 1825 3862 1825
Size: 2037 2273
BlpI GC'TnA_GC
Cuts at: 1422 1422
Size: 4310
Bme1580I G_kGCm'C
Cuts at: 149 415 459 1935 2028 3084 149
Size: 266 44 1476 93 1056 1375
Fragments arranged by size:
1476 1375 1056 266 93 44
BmrI ACTGGGnnnn_n'
Cuts at: 1522 1603 1938 4082 1522
Size: 81 335 2144 1750
Fragments arranged by size:
2144 1750 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 633 993 2677 633
Size: 360 1684 2266
Fragments arranged by size:
2266 1684 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 415 782 1156 409
Size: 6 367 374 3563
Fragments arranged by size:
3563 374 367 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 718 1486 2365 2857 3155 646
Size: 72 768 879 492 298 1801
Fragments arranged by size:
1801 879 768 492 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 4014 4014
Size: 4310
BsaAI yAC'GTr
Cuts at: 204 549 1663 204
Size: 345 1114 2851
Fragments arranged by size:
2851 1114 345
BsaBI GATnn'nnATC
Cuts at: 6 953 1181 1824 3861 6
Size: 947 228 643 2037 455
Fragments arranged by size:
2037 947 643 455 228
BsaHI Gr'CG_yC
Cuts at: 470 1741 1989 2691 4187 470
Size: 1271 248 702 1496 593
Fragments arranged by size:
1496 1271 702 593 248
BsaWI w'CCGG_w
Cuts at: 583 1499 2020 2698 2972 3119 3530 583
Size: 916 521 678 274 147 411 1363
Fragments arranged by size:
1363 916 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 172 202 673 703 172
Size: 30 471 30 3779
Fragments arranged by size:
3779 471 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 711 763 907 1012 711
Size: 52 144 105 4009
Fragments arranged by size:
4009 144 105 52
BseYI C'CCAG_C
Cuts at: 211 302 408 571 696 1735 2493 3070 3566
Size: 91 106 163 125 1039 758 577 496
Cuts at: 3566 211
Size: 955
Fragments arranged by size:
1039 955 758 577 496 163 125 106
91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 295 232
Size: 63 4247
BsiEI CG_ry'CG
Cuts at: 104 1852 1898 3106 104
Size: 1748 46 1208 1308
Fragments arranged by size:
1748 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 619 1269 1324 2102 2292 3084 619
Size: 650 55 778 190 792 1845
Fragments arranged by size:
1845 792 778 650 190 55
Bsp1286I G_dGCh'C
Cuts at: 149 284 415 459 619 718 916 1269 1324
Size: 135 131 44 160 99 198 353 55
Cuts at: 1324 1935 2028 2102 2292 2354 3084 149
Size: 611 93 74 190 62 730 1375
Fragments arranged by size:
1375 730 611 353 198 190 160 135
131 99 93 74 62 55 44
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 677 701 1360 1574 1876 2257 677
Size: 24 659 214 302 381 2730
Fragments arranged by size:
2730 659 381 302 214 24
BsrBI CCG'CTC
Cuts at: 50 566 2602 2656 4140 4305 50
Size: 516 2036 54 1484 165 55
Fragments arranged by size:
2036 1484 516 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2222 2222
Size: 4310
BsrFI r'CCGG_y
Cuts at: 433 583 708 2308 2489 2698 433
Size: 150 125 1600 181 209 2045
Fragments arranged by size:
2045 1600 209 181 150 125
BssSI C'ACGA_G
Cuts at: 633 2581 2939 633
Size: 1948 358 2004
Fragments arranged by size:
2004 1948 358
BstAPI GCAn_nnn'nTGC
Cuts at: 266 1687 266
Size: 1421 2889
BstBI TT'CG_AA
Cuts at: 1296 2671 1296
Size: 1375 2935
BstYI r'GATC_y
Cuts at: 1 1089 1335 1802 1825 2160 2406 3418 3504
Size: 1088 246 467 23 335 246 1012 86
Cuts at: 3504 3516 3862 1
Size: 12 346 449
Fragments arranged by size:
1088 1012 467 449 346 335 246 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 968 968
Size: 4310
BtgI C'CryG_G
Cuts at: 107 473 764 107
Size: 366 291 3653
Fragments arranged by size:
3653 366 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 133 619 2260 2441 2604 133
Size: 486 1641 181 163 1839
Fragments arranged by size:
1839 1641 486 181 163
BtsI GCAGTG_nn'
Cuts at: 1553 1553
Size: 4310
DraI TTT'AAA
Cuts at: 1309 1309
Size: 4310
DraIII CAC_nnn'GTG
Cuts at: 266 266
Size: 4310
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 218 320 461 1895 2069 2460 2487 3832 218
Size: 102 141 1434 174 391 27 1345 696
Fragments arranged by size:
1434 1345 696 391 174 141 102 27
EagI C'GGCC_G
Cuts at: 1895 1895
Size: 4310
EarI CTCTTCn'nnn_
Cuts at: 607 809 2333 2543 607
Size: 202 1524 210 2374
Fragments arranged by size:
2374 1524 210 202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 442 519 760 1137 2518 2828 2974 4120
Size: 315 77 241 377 1381 310 146 1146
Cuts at: 4120 127
Size: 317
Fragments arranged by size:
1381 1146 377 317 315 310 241 146
77
EcoICRI GAG'CTC
Cuts at: 617 1322 617
Size: 705 3605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 415 667 782 830 1156 2135 2567 3314
Size: 6 252 115 48 326 979 432 747
Cuts at: 3314 3582 409
Size: 268 1137
Fragments arranged by size:
1137 979 747 432 326 268 252 115
48 6
EcoNI CCTnn'n_nnAGG
Cuts at: 463 463
Size: 4310
EcoO109I rG'GnC_Cy
Cuts at: 412 455 456 753 1449 412
Size: 43 1 297 696 3273
Fragments arranged by size:
3273 696 297 43 1
EcoRI G'AATT_C
Cuts at: 1314 1314
Size: 4310
EcoRV GAT'ATC
Cuts at: 1183 1183
Size: 4310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1101 2329 1101
Size: 1228 3082
FspI TGC'GCA
Cuts at: 2091 2091
Size: 4310
HaeII r_GCGC'y
Cuts at: 319 473 1744 1992 3014 3993 319
Size: 154 1271 248 1022 979 636
Fragments arranged by size:
1271 1022 979 636 248 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 172 204 328 360 421 453 2152 2182 2184
Size: 32 124 32 61 32 1699 30 2
Cuts at: 2184 2214 172
Size: 30 2268
Fragments arranged by size:
2268 1699 124 61 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 236 1349 236
Size: 1113 3197
KasI G'GCGC_C
Cuts at: 469 1740 1988 469
Size: 1271 248 2791
Fragments arranged by size:
2791 1271 248
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2981 3165 2981
Size: 184 4126
MscI TGG'CCA
Cuts at: 2071 2071
Size: 4310
MslI CAynn'nnrTG
Cuts at: 2299 2426 2299
Size: 127 4183
MspA1I CmG'CkG
Cuts at: 475 492 766 1421 1735 2095 3108 3353 475
Size: 17 274 655 314 360 1013 245 1432
Fragments arranged by size:
1432 1013 655 360 314 274 245 17
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 470 1741 1989 470
Size: 1271 248 2791
Fragments arranged by size:
2791 1271 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4310
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NspI r_CATG'y
Cuts at: 1363 1863 2770 1363
Size: 500 907 2903
Fragments arranged by size:
2903 907 500
PciI A'CATG_T
Cuts at: 2766 2766
Size: 4310
PfoI T'CCnGG_A
Cuts at: 359 836 359
Size: 477 3833
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PpuMI rG'GwC_Cy
Cuts at: 753 753
Size: 4310
PspOMI G'GGCC_C
Cuts at: 145 411 455 145
Size: 266 44 4000
Fragments arranged by size:
4000 266 44
PstI C_TGCA'G
Cuts at: 1357 2042 1357
Size: 685 3625
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 1735 2095 1735
Size: 360 3950
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 619 1324 619
Size: 705 3605
SacII CC_GC'GG
Cuts at: 476 767 476
Size: 291 4019
SalI G'TCGA_C
Cuts at: 234 1347 234
Size: 1113 3197
SapI GCTCTTCn'nnn_
Cuts at: 607 2333 2543 607
Size: 1726 210 2374
Fragments arranged by size:
2374 1726 210
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 3827 3827
Size: 4310
SfcI C'TryA_G
Cuts at: 33 1353 2038 3031 3222 33
Size: 1320 685 993 191 1121
Fragments arranged by size:
1320 1121 993 685 191
SfoI GGC'GCC
Cuts at: 471 1742 1990 471
Size: 1271 248 2791
Fragments arranged by size:
2791 1271 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SgrAI Cr'CCGG_yG
Cuts at: 708 708
Size: 4310
SmaI CCC'GGG
Cuts at: 285 309 453 883 1332 285
Size: 24 144 430 449 3263
Fragments arranged by size:
3263 449 430 144 24
SmlI C'TyrA_G
Cuts at: 661 733 1465 2380 2872 3134 661
Size: 72 732 915 492 262 1837
Fragments arranged by size:
1837 915 732 492 262 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 1363 1863 1363
Size: 500 3810
StuI AGG'CCT
Cuts at: 328 400 1111 328
Size: 72 711 3527
Fragments arranged by size:
3527 711 72
StyI C'CwwG_G
Cuts at: 107 1444 1798 107
Size: 1337 354 2619
Fragments arranged by size:
2619 1337 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 166 975 1893 2026 2485 2694 166
Size: 809 918 133 459 209 1782
Fragments arranged by size:
1782 918 809 459 209 133
TatI w'GTAC_w
Cuts at: 353 3825 353
Size: 3472 838
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1097 1129 3827 3859 1097
Size: 32 2698 32 1548
Fragments arranged by size:
2698 1548 32 32
Tth111I GACn'n_nGTC
Cuts at: 2107 2107
Size: 4310
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1245 1805 1245
Size: 560 3750
XmaI C'CCGG_G
Cuts at: 283 307 451 881 1330 283
Size: 24 144 430 449 3263
Fragments arranged by size:
3263 449 430 144 24
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI