Restriction map for
pF1KB8123
(Circular) (Six-base) MAPSORT of: pF1KB8123.seq Check: 5904 from: 1 to: 4664
pF1KB8123 4664 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 543 543
Size: 4664
AatII G_ACGT'C
Cuts at: 688 688
Size: 4664
AccI GT'mk_AC
Cuts at: 456 962 1651 1702 456
Size: 506 689 51 3418
Fragments arranged by size:
3418 689 506 51
Acc65I G'GTAC_C
Cuts at: 1680 1680
Size: 4664
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 1418 2489 2921 3668 3936 875
Size: 543 1071 432 747 268 1603
Fragments arranged by size:
1603 1071 747 543 432 268
AflIII A'CryG_T
Cuts at: 2016 3120 4097 2016
Size: 1104 977 2583
Fragments arranged by size:
2583 1104 977
AgeI A'CCGG_T
Cuts at: 3052 3052
Size: 4664
AlwNI CAG_nnn'CTG
Cuts at: 1969 3536 1969
Size: 1567 3097
ApaLI G'TGCA_C
Cuts at: 3434 3434
Size: 4664
ApoI r'AATT_y
Cuts at: 146 1411 1459 1510 1668 4017 4273 146
Size: 1265 48 51 158 2349 256 537
Fragments arranged by size:
2349 1265 537 256 158 51 48
AseI AT'TA_AT
Cuts at: 20 440 20
Size: 420 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 983 1684 983
Size: 701 3963
BamHI G'GATC_C
Cuts at: 1689 1689
Size: 4664
BanI G'GyrC_C
Cuts at: 598 1065 1680 2094 2342 2377 598
Size: 467 615 414 248 35 2885
Fragments arranged by size:
2885 615 467 414 248 35
BanII G_rGCy'C
Cuts at: 1045 1678 2708 1045
Size: 633 1030 3001
Fragments arranged by size:
3001 1030 633
BbeI G_GCGC'C
Cuts at: 2098 2346 2098
Size: 248 4416
BbsI GAAGACnn'nnnn_
Cuts at: 297 863 297
Size: 566 4098
BciVI GTATCCnnnnn_n'
Cuts at: 1286 2562 3323 4664 1286
Size: 1276 761 1341 1286
Fragments arranged by size:
1341 1286 1276 761
BclI T'GATC_A
Cuts at: 2184 2184
Size: 4664
BfrBI ATG'CAT
Cuts at: 1312 1312
Size: 4664
BglII A'GATC_T
Cuts at: 2179 4216 2179
Size: 2037 2627
BlpI GC'TnA_GC
Cuts at: 1776 1776
Size: 4664
Bme1580I G_kGCm'C
Cuts at: 770 2289 2382 3438 770
Size: 1519 93 1056 1996
Fragments arranged by size:
1996 1519 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 839 1239 1876 1957 2292 4436 839
Size: 400 637 81 335 2144 1067
Fragments arranged by size:
2144 1067 637 400 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 4664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 674 985 1055 512
Size: 162 311 70 4121
Fragments arranged by size:
4121 311 162 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 717 1126 1840 2719 3211 3509 717
Size: 409 714 879 492 298 1872
Fragments arranged by size:
1872 879 714 492 409 298
BsaI GGTCTCn'nnnn_
Cuts at: 4368 4368
Size: 4664
BsaAI yAC'GTr
Cuts at: 2017 2017
Size: 4664
BsaBI GATnn'nnATC
Cuts at: 6 2178 4215 6
Size: 2172 2037 455
Fragments arranged by size:
2172 2037 455
BsaHI Gr'CG_yC
Cuts at: 685 2095 2343 3045 4541 685
Size: 1410 248 702 1496 808
Fragments arranged by size:
1496 1410 808 702 248
BsaWI w'CCGG_w
Cuts at: 1853 2374 3052 3326 3473 3884 1853
Size: 521 678 274 147 411 2633
Fragments arranged by size:
2633 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 485 515 591 621 1195 1225 485
Size: 30 76 30 574 30 3924
Fragments arranged by size:
3924 574 76 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 344 594 599 145
Size: 199 250 5 4210
Fragments arranged by size:
4210 250 199 5
BseYI C'CCAG_C
Cuts at: 2089 2847 3424 3920 2089
Size: 758 577 496 2833
Fragments arranged by size:
2833 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 810 1207 178
Size: 632 397 3635
Fragments arranged by size:
3635 632 397
BsiEI CG_ry'CG
Cuts at: 104 2206 2252 3460 104
Size: 2102 46 1208 1308
Fragments arranged by size:
2102 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 534 579 1678 2456 2646 3438 534
Size: 45 1099 778 190 792 1760
Fragments arranged by size:
1760 1099 792 778 190 45
BsmI GAATG_Cn'
Cuts at: 492 798 1541 492
Size: 306 743 3615
Fragments arranged by size:
3615 743 306
BsmBI CGTCTCn'nnnn_
Cuts at: 692 692
Size: 4664
Bsp1286I G_dGCh'C
Cuts at: 534 579 770 1045 1678 2289 2382 2456 2646
Size: 45 191 275 633 611 93 74 190
Cuts at: 2646 2708 3438 534
Size: 62 730 1760
Fragments arranged by size:
1760 730 633 611 275 191 190 93
74 62 45
BspEI T'CCGG_A
Cuts at: 3884 3884
Size: 4664
BspHI T'CATG_A
Cuts at: 3840 3840
Size: 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 543 1163 1714 1928 2230 2611 543
Size: 620 551 214 302 381 2596
Fragments arranged by size:
2596 620 551 381 302 214
BsrBI CCG'CTC
Cuts at: 50 385 2956 3010 4494 4659 50
Size: 335 2571 54 1484 165 55
Fragments arranged by size:
2571 1484 335 165 55 54
BsrDI GCAATG_nn'
Cuts at: 806 2576 806
Size: 1770 2894
BsrFI r'CCGG_y
Cuts at: 2662 2843 3052 2662
Size: 181 209 4274
Fragments arranged by size:
4274 209 181
BssSI C'ACGA_G
Cuts at: 1635 2935 3293 1635
Size: 1300 358 3006
Fragments arranged by size:
3006 1300 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2041 2041
Size: 4664
BstBI TT'CG_AA
Cuts at: 3025 3025
Size: 4664
BstEII G'GTnAC_C
Cuts at: 166 279 166
Size: 113 4551
BstXI CCAn_nnnn'nTGG
Cuts at: 595 595
Size: 4664
BstYI r'GATC_y
Cuts at: 1 1136 1689 2156 2179 2514 2760 3772 3858
Size: 1135 553 467 23 335 246 1012 86
Cuts at: 3858 3870 4216 1
Size: 12 346 449
Fragments arranged by size:
1135 1012 553 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1652 1652
Size: 4664
Bsu36I CC'TnA_GG
Cuts at: 1036 1036
Size: 4664
BtgI C'CryG_G
Cuts at: 107 107
Size: 4664
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 726 2614 2795 2958 726
Size: 1888 181 163 2432
Fragments arranged by size:
2432 1888 181 163
BtsI GCAGTG_nn'
Cuts at: 600 1536 1907 600
Size: 936 371 3357
Fragments arranged by size:
3357 936 371
DraI TTT'AAA
Cuts at: 524 1663 524
Size: 1139 3525
DrdI GACnn_nn'nnGTC
Cuts at: 2370 3228 2370
Size: 858 3806
EaeI y'GGCC_r
Cuts at: 679 2249 2423 2814 2841 4186 679
Size: 1570 174 391 27 1345 1157
Fragments arranged by size:
1570 1345 1157 391 174 27
EagI C'GGCC_G
Cuts at: 2249 2249
Size: 4664
EarI CTCTTCn'nnn_
Cuts at: 2687 2897 2687
Size: 210 4454
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2872 3182 3328 4474 2872
Size: 310 146 1146 3062
Fragments arranged by size:
3062 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1676 1676
Size: 4664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 674 875 985 1055 1418 2489 2921 3668
Size: 162 201 110 70 363 1071 432 747
Cuts at: 3668 3936 512
Size: 268 1240
Fragments arranged by size:
1240 1071 747 432 363 268 201 162
110 70
EcoO109I rG'GnC_Cy
Cuts at: 1206 1803 1206
Size: 597 4067
EcoRI G'AATT_C
Cuts at: 1668 1668
Size: 4664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4664
FspI TGC'GCA
Cuts at: 2445 2445
Size: 4664
HaeII r_GCGC'y
Cuts at: 2098 2346 3368 4347 2098
Size: 248 1022 979 2415
Fragments arranged by size:
2415 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 649 681 2506 2536 2538 2568 649
Size: 32 1825 30 2 30 2745
Fragments arranged by size:
2745 1825 32 30 30 2
HincII GTy'rAC
Cuts at: 867 1703 867
Size: 836 3828
KasI G'GCGC_C
Cuts at: 2094 2342 2094
Size: 248 4416
KpnI G_GTAC'C
Cuts at: 1684 1684
Size: 4664
MfeI C'AATT_G
Cuts at: 1346 1346
Size: 4664
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1487 3335 3519 1487
Size: 1848 184 2632
Fragments arranged by size:
2632 1848 184
MscI TGG'CCA
Cuts at: 2425 2425
Size: 4664
MslI CAynn'nnrTG
Cuts at: 214 442 666 811 2653 2780 214
Size: 228 224 145 1842 127 2098
Fragments arranged by size:
2098 1842 228 224 145 127
MspA1I CmG'CkG
Cuts at: 1775 2089 2449 3462 3707 1775
Size: 314 360 1013 245 2732
Fragments arranged by size:
2732 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2845 2845
Size: 4664
NarI GG'CG_CC
Cuts at: 2095 2343 2095
Size: 248 4416
NcoI C'CATG_G
Cuts at: 107 107
Size: 4664
NgoMIV G'CCGG_C
Cuts at: 2843 2843
Size: 4664
NsiI A_TGCA'T
Cuts at: 1314 1314
Size: 4664
NspI r_CATG'y
Cuts at: 575 1316 1717 2217 3124 575
Size: 741 401 500 907 2115
Fragments arranged by size:
2115 907 741 500 401
PciI A'CATG_T
Cuts at: 3120 3120
Size: 4664
PfoI T'CCnGG_A
Cuts at: 221 1001 221
Size: 780 3884
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4632
PpuMI rG'GwC_Cy
Cuts at: 1206 1206
Size: 4664
PstI C_TGCA'G
Cuts at: 161 1711 2396 161
Size: 1550 685 2429
Fragments arranged by size:
2429 1550 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 2089 2449 2089
Size: 360 4304
RsrII CG'GwC_CG
Cuts at: 2859 2859
Size: 4664
SacI G_AGCT'C
Cuts at: 1678 1678
Size: 4664
SalI G'TCGA_C
Cuts at: 1701 1701
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 2687 2897 2687
Size: 210 4454
SbfI CC_TGCA'GG
Cuts at: 1711 1711
Size: 4664
ScaI AGT'ACT
Cuts at: 4181 4181
Size: 4664
SfcI C'TryA_G
Cuts at: 33 157 424 1707 2392 3385 3576 33
Size: 124 267 1283 685 993 191 1121
Fragments arranged by size:
1283 1121 993 685 267 191 124
SfoI GGC'GCC
Cuts at: 2096 2344 2096
Size: 248 4416
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SmaI CCC'GGG
Cuts at: 985 1686 985
Size: 701 3963
SmlI C'TyrA_G
Cuts at: 696 1141 1819 2734 3226 3488 696
Size: 445 678 915 492 262 1872
Fragments arranged by size:
1872 915 678 492 445 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 1717 2217 1717
Size: 500 4164
SspI AAT'ATT
Cuts at: 918 918
Size: 4664
StyI C'CwwG_G
Cuts at: 107 371 1562 1798 2152 107
Size: 264 1191 236 354 2619
Fragments arranged by size:
2619 1191 354 264 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1178 2247 2380 2839 3048 1178
Size: 1069 133 459 209 2794
Fragments arranged by size:
2794 1069 459 209 133
TatI w'GTAC_w
Cuts at: 1013 1211 4179 1013
Size: 198 2968 1498
Fragments arranged by size:
2968 1498 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 485 517 524 556 591 623 4181 4213 485
Size: 32 7 32 35 32 3558 32 936
Fragments arranged by size:
3558 936 35 32 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 2461 2461
Size: 4664
XbaI T'CTAG_A
Cuts at: 1278 1695 1278
Size: 417 4247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 698 1079 2159 698
Size: 381 1080 3203
Fragments arranged by size:
3203 1080 381
XmaI C'CCGG_G
Cuts at: 983 1684 983
Size: 701 3963
XmnI GAAnn'nnTTC
Cuts at: 470 1108 470
Size: 638 4026
ZraI GAC'GTC
Cuts at: 686 686
Size: 4664
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsiWI BsrGI BssHII ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI