Restriction map for
pF1KB8122
(Circular) (Six-base) MAPSORT of: pF1KB8122.seq Check: 8289 from: 1 to: 3755
pF1KB8122 3755 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 793 793
Size: 3755
Acc65I G'GTAC_C
Cuts at: 771 771
Size: 3755
AclI AA'CG_TT
Cuts at: 465 3595 465
Size: 3130 625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 1580 2012 2759 3027 186
Size: 1394 432 747 268 914
Fragments arranged by size:
1394 914 747 432 268
AflIII A'CryG_T
Cuts at: 1107 2211 3188 1107
Size: 1104 977 1674
Fragments arranged by size:
1674 1104 977
AgeI A'CCGG_T
Cuts at: 2143 2143
Size: 3755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 3755
AlwNI CAG_nnn'CTG
Cuts at: 1060 2627 1060
Size: 1567 2188
ApaLI G'TGCA_C
Cuts at: 2525 2525
Size: 3755
ApoI r'AATT_y
Cuts at: 759 3108 3364 759
Size: 2349 256 1150
Fragments arranged by size:
2349 1150 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
AvaI C'yCGr_G
Cuts at: 775 775
Size: 3755
BamHI G'GATC_C
Cuts at: 780 780
Size: 3755
BanI G'GyrC_C
Cuts at: 609 771 1185 1433 1468 609
Size: 162 414 248 35 2896
Fragments arranged by size:
2896 414 248 162 35
BanII G_rGCy'C
Cuts at: 176 697 769 1799 176
Size: 521 72 1030 2132
Fragments arranged by size:
2132 1030 521 72
BbeI G_GCGC'C
Cuts at: 1189 1437 1189
Size: 248 3507
BciVI GTATCCnnnnn_n'
Cuts at: 1653 2414 3755 1653
Size: 761 1341 1653
Fragments arranged by size:
1653 1341 761
BclI T'GATC_A
Cuts at: 1275 1275
Size: 3755
BfrBI ATG'CAT
Cuts at: 285 285
Size: 3755
BglII A'GATC_T
Cuts at: 1270 3307 1270
Size: 2037 1718
BlpI GC'TnA_GC
Cuts at: 867 867
Size: 3755
Bme1580I G_kGCm'C
Cuts at: 614 1380 1473 2529 614
Size: 766 93 1056 1840
Fragments arranged by size:
1840 1056 766 93
BmrI ACTGGGnnnn_n'
Cuts at: 403 967 1048 1383 3527 403
Size: 564 81 335 2144 631
Fragments arranged by size:
2144 631 564 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 3755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 340 592 235
Size: 105 252 3398
Fragments arranged by size:
3398 252 105
Bpu10I CC'TnA_GC
Cuts at: 435 435
Size: 3755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1810 2302 2600 931
Size: 879 492 298 2086
Fragments arranged by size:
2086 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 384 3459 384
Size: 3075 680
BsaAI yAC'GTr
Cuts at: 1108 1108
Size: 3755
BsaBI GATnn'nnATC
Cuts at: 6 1269 3306 6
Size: 1263 2037 455
Fragments arranged by size:
2037 1263 455
BsaHI Gr'CG_yC
Cuts at: 1186 1434 2136 3632 1186
Size: 248 702 1496 1309
Fragments arranged by size:
1496 1309 702 248
BsaWI w'CCGG_w
Cuts at: 944 1465 2143 2417 2564 2975 944
Size: 521 678 274 147 411 1724
Fragments arranged by size:
1724 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 292 508 511 514 532 535 589 292
Size: 216 3 3 18 3 54 3458
Fragments arranged by size:
3458 216 54 18 3 3 3
BseYI C'CCAG_C
Cuts at: 209 306 1180 1938 2515 3011 209
Size: 97 874 758 577 496 953
Fragments arranged by size:
953 874 758 577 496 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 415
Size: 3755
BsiEI CG_ry'CG
Cuts at: 104 155 1297 1343 2551 104
Size: 51 1142 46 1208 1308
Fragments arranged by size:
1308 1208 1142 51 46
BsiHKAI G_wGCw'C
Cuts at: 161 176 769 1547 1737 2529 161
Size: 15 593 778 190 792 1387
Fragments arranged by size:
1387 792 778 593 190 15
Bsp1286I G_dGCh'C
Cuts at: 161 176 614 697 769 1380 1473 1547 1737
Size: 15 438 83 72 611 93 74 190
Cuts at: 1737 1799 2529 161
Size: 62 730 1387
Fragments arranged by size:
1387 730 611 438 190 93 83 74
72 62 15
BspEI T'CCGG_A
Cuts at: 2975 2975
Size: 3755
BspHI T'CATG_A
Cuts at: 2931 2931
Size: 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 805 1019 1321 1702 805
Size: 214 302 381 2858
Fragments arranged by size:
2858 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2047 2101 3585 3750 50
Size: 1997 54 1484 165 55
Fragments arranged by size:
1997 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 344 1667 344
Size: 1323 2432
BsrFI r'CCGG_y
Cuts at: 336 1753 1934 2143 336
Size: 1417 181 209 1948
Fragments arranged by size:
1948 1417 209 181
BssSI C'ACGA_G
Cuts at: 2026 2384 2026
Size: 358 3397
BstAPI GCAn_nnn'nTGC
Cuts at: 1132 1132
Size: 3755
BstBI TT'CG_AA
Cuts at: 2116 2116
Size: 3755
BstEII G'GTnAC_C
Cuts at: 339 339
Size: 3755
BstYI r'GATC_y
Cuts at: 1 296 780 1247 1270 1605 1851 2863 2949
Size: 295 484 467 23 335 246 1012 86
Cuts at: 2949 2961 3307 1
Size: 12 346 449
Fragments arranged by size:
1012 484 467 449 346 335 295 246
86 23 12
BtgI C'CryG_G
Cuts at: 374 383 374
Size: 9 3746
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 271 1705 1886 2049 271
Size: 1434 181 163 1977
Fragments arranged by size:
1977 1434 181 163
BtsI GCAGTG_nn'
Cuts at: 152 998 152
Size: 846 2909
DraI TTT'AAA
Cuts at: 754 754
Size: 3755
DrdI GACnn_nn'nnGTC
Cuts at: 1461 2319 1461
Size: 858 2897
EaeI y'GGCC_r
Cuts at: 456 1340 1514 1905 1932 3277 456
Size: 884 174 391 27 1345 934
Fragments arranged by size:
1345 934 884 391 174 27
EagI C'GGCC_G
Cuts at: 1340 1340
Size: 3755
EarI CTCTTCn'nnn_
Cuts at: 521 527 656 1778 1988 521
Size: 6 129 1122 210 2288
Fragments arranged by size:
2288 1122 210 129 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 204 1963 2273 2419 3565 204
Size: 1759 310 146 1146 394
Fragments arranged by size:
1759 1146 394 310 146
EcoICRI GAG'CTC
Cuts at: 174 767 174
Size: 593 3162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 235 340 592 1580 2012 2759 3027 186
Size: 49 105 252 988 432 747 268 914
Fragments arranged by size:
988 914 747 432 268 252 105 49
EcoO109I rG'GnC_Cy
Cuts at: 439 894 439
Size: 455 3300
EcoRI G'AATT_C
Cuts at: 759 759
Size: 3755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1774 1774
Size: 3755
FspI TGC'GCA
Cuts at: 1536 1536
Size: 3755
HaeII r_GCGC'y
Cuts at: 125 1189 1437 2459 3438 125
Size: 1064 248 1022 979 442
Fragments arranged by size:
1064 1022 979 442 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1597 1627 1629 1659 1597
Size: 30 2 30 3693
Fragments arranged by size:
3693 30 30 2
HincII GTy'rAC
Cuts at: 794 794
Size: 3755
KasI G'GCGC_C
Cuts at: 1185 1433 1185
Size: 248 3507
KpnI G_GTAC'C
Cuts at: 775 775
Size: 3755
MluI A'CGCG_T
Cuts at: 3188 3188
Size: 3755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2610 2426
Size: 184 3571
MscI TGG'CCA
Cuts at: 458 1516 458
Size: 1058 2697
MslI CAynn'nnrTG
Cuts at: 1744 1871 1744
Size: 127 3628
MspA1I CmG'CkG
Cuts at: 213 866 1180 1540 2553 2798 213
Size: 653 314 360 1013 245 1170
Fragments arranged by size:
1170 1013 653 360 314 245
NaeI GCC'GGC
Cuts at: 1936 1936
Size: 3755
NarI GG'CG_CC
Cuts at: 1186 1434 1186
Size: 248 3507
NcoI C'CATG_G
Cuts at: 374 383 374
Size: 9 3746
NgoMIV G'CCGG_C
Cuts at: 1934 1934
Size: 3755
NsiI A_TGCA'T
Cuts at: 287 287
Size: 3755
NspI r_CATG'y
Cuts at: 808 1308 2215 808
Size: 500 907 2348
Fragments arranged by size:
2348 907 500
PciI A'CATG_T
Cuts at: 2211 2211
Size: 3755
PmeI GTTT'AAAC
Cuts at: 754 754
Size: 3755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 3723
PpuMI rG'GwC_Cy
Cuts at: 439 439
Size: 3755
PstI C_TGCA'G
Cuts at: 235 802 1487 235
Size: 567 685 2503
Fragments arranged by size:
2503 685 567
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3755
PvuII CAG'CTG
Cuts at: 213 1180 1540 213
Size: 967 360 2428
Fragments arranged by size:
2428 967 360
RsrII CG'GwC_CG
Cuts at: 1950 1950
Size: 3755
SacI G_AGCT'C
Cuts at: 176 769 176
Size: 593 3162
SalI G'TCGA_C
Cuts at: 792 792
Size: 3755
SapI GCTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
SbfI CC_TGCA'GG
Cuts at: 802 802
Size: 3755
ScaI AGT'ACT
Cuts at: 3272 3272
Size: 3755
SfcI C'TryA_G
Cuts at: 33 231 798 1483 2476 2667 33
Size: 198 567 685 993 191 1121
Fragments arranged by size:
1121 993 685 567 198 191
SfoI GGC'GCC
Cuts at: 1187 1435 1187
Size: 248 3507
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
SmaI CCC'GGG
Cuts at: 777 777
Size: 3755
SmlI C'TyrA_G
Cuts at: 910 1825 2317 2579 910
Size: 915 492 262 2086
Fragments arranged by size:
2086 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3755
SphI G_CATG'C
Cuts at: 808 1308 808
Size: 500 3255
StuI AGG'CCT
Cuts at: 361 361
Size: 3755
StyI C'CwwG_G
Cuts at: 374 383 889 1243 374
Size: 9 506 354 2886
Fragments arranged by size:
2886 506 354 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 1471 1930 2139 1338
Size: 133 459 209 2954
Fragments arranged by size:
2954 459 209 133
TatI w'GTAC_w
Cuts at: 3270 3270
Size: 3755
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 3723
Tth111I GACn'n_nGTC
Cuts at: 1552 1552
Size: 3755
XbaI T'CTAG_A
Cuts at: 786 786
Size: 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 381 427 1250 381
Size: 46 823 2886
Fragments arranged by size:
2886 823 46
XmaI C'CCGG_G
Cuts at: 775 775
Size: 3755
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI