Restriction map for
pF1KB8121
(Circular) (Six-base) MAPSORT of: pF1KB8121.seq Check: 2720 from: 1 to: 4073
pF1KB8121 4073 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1111 1111
Size: 4073
Acc65I G'GTAC_C
Cuts at: 1089 1089
Size: 4073
AclI AA'CG_TT
Cuts at: 3913 3913
Size: 4073
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 756 1898 2330 3077 3345 476
Size: 280 1142 432 747 268 1204
Fragments arranged by size:
1204 1142 747 432 280 268
AfeI AGC'GCT
Cuts at: 265 265
Size: 4073
AflIII A'CryG_T
Cuts at: 1425 2529 3506 1425
Size: 1104 977 1992
Fragments arranged by size:
1992 1104 977
AgeI A'CCGG_T
Cuts at: 2461 2461
Size: 4073
AleI CACnn'nnGTG
Cuts at: 629 629
Size: 4073
AlwNI CAG_nnn'CTG
Cuts at: 1378 2945 1378
Size: 1567 2506
ApaLI G'TGCA_C
Cuts at: 2843 2843
Size: 4073
ApoI r'AATT_y
Cuts at: 921 945 1077 3426 3682 921
Size: 24 132 2349 256 1312
Fragments arranged by size:
2349 1312 256 132 24
AseI AT'TA_AT
Cuts at: 20 20
Size: 4073
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
AvaI C'yCGr_G
Cuts at: 1093 1093
Size: 4073
BamHI G'GATC_C
Cuts at: 1098 1098
Size: 4073
BanI G'GyrC_C
Cuts at: 403 1089 1503 1751 1786 403
Size: 686 414 248 35 2690
Fragments arranged by size:
2690 686 414 248 35
BanII G_rGCy'C
Cuts at: 137 1087 2117 137
Size: 950 1030 2093
Fragments arranged by size:
2093 1030 950
BbeI G_GCGC'C
Cuts at: 1507 1755 1507
Size: 248 3825
BbsI GAAGACnn'nnnn_
Cuts at: 455 908 455
Size: 453 3620
BbvCI CC'TCA_GC
Cuts at: 145 145
Size: 4073
BciVI GTATCCnnnnn_n'
Cuts at: 1971 2732 4073 1971
Size: 761 1341 1971
Fragments arranged by size:
1971 1341 761
BclI T'GATC_A
Cuts at: 1593 1593
Size: 4073
BglI GCCn_nnn'nGGC
Cuts at: 974 974
Size: 4073
BglII A'GATC_T
Cuts at: 1588 3625 1588
Size: 2037 2036
BlpI GC'TnA_GC
Cuts at: 1185 1185
Size: 4073
Bme1580I G_kGCm'C
Cuts at: 408 1698 1791 2847 408
Size: 1290 93 1056 1634
Fragments arranged by size:
1634 1290 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1285 1366 1701 3845 1285
Size: 81 335 2144 1513
Fragments arranged by size:
2144 1513 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 363 252
Size: 111 3962
Bpu10I CC'TnA_GC
Cuts at: 145 145
Size: 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 2128 2620 2918 1249
Size: 879 492 298 2404
Fragments arranged by size:
2404 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3777 3777
Size: 4073
BsaAI yAC'GTr
Cuts at: 1426 1426
Size: 4073
BsaBI GATnn'nnATC
Cuts at: 6 511 1587 3624 6
Size: 505 1076 2037 455
Fragments arranged by size:
2037 1076 505 455
BsaHI Gr'CG_yC
Cuts at: 1504 1752 2454 3950 1504
Size: 248 702 1496 1627
Fragments arranged by size:
1627 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1262 1783 2461 2735 2882 3293 1262
Size: 521 678 274 147 411 2042
Fragments arranged by size:
2042 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 562 592 562
Size: 30 4043
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 213 319 213
Size: 106 3967
BseYI C'CCAG_C
Cuts at: 1498 2256 2833 3329 1498
Size: 758 577 496 2242
Fragments arranged by size:
2242 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 759 1615 1661 2869 104
Size: 655 856 46 1208 1308
Fragments arranged by size:
1308 1208 856 655 46
BsiHKAI G_wGCw'C
Cuts at: 1087 1865 2055 2847 1087
Size: 778 190 792 2313
Fragments arranged by size:
2313 792 778 190
BsmI GAATG_Cn'
Cuts at: 330 330
Size: 4073
Bsp1286I G_dGCh'C
Cuts at: 137 408 1087 1698 1791 1865 2055 2117 2847
Size: 271 679 611 93 74 190 62 730
Cuts at: 2847 137
Size: 1363
Fragments arranged by size:
1363 730 679 611 271 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3293 3293
Size: 4073
BspHI T'CATG_A
Cuts at: 515 3249 515
Size: 2734 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1123 1337 1639 2020 1123
Size: 214 302 381 3176
Fragments arranged by size:
3176 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2365 2419 3903 4068 50
Size: 2315 54 1484 165 55
Fragments arranged by size:
2315 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 220 1985 220
Size: 1765 2308
BsrFI r'CCGG_y
Cuts at: 2071 2252 2461 2071
Size: 181 209 3683
Fragments arranged by size:
3683 209 181
BssSI C'ACGA_G
Cuts at: 2344 2702 2344
Size: 358 3715
BstAPI GCAn_nnn'nTGC
Cuts at: 1450 1450
Size: 4073
BstBI TT'CG_AA
Cuts at: 2434 2434
Size: 4073
BstYI r'GATC_y
Cuts at: 1 249 1098 1565 1588 1923 2169 3181 3267
Size: 248 849 467 23 335 246 1012 86
Cuts at: 3267 3279 3625 1
Size: 12 346 449
Fragments arranged by size:
1012 849 467 449 346 335 248 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 196 196
Size: 4073
BtgI C'CryG_G
Cuts at: 581 801 581
Size: 220 3853
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2023 2204 2367 2023
Size: 181 163 3729
Fragments arranged by size:
3729 181 163
BtsI GCAGTG_nn'
Cuts at: 537 831 1059 1316 537
Size: 294 228 257 3294
Fragments arranged by size:
3294 294 257 228
DraI TTT'AAA
Cuts at: 1072 1072
Size: 4073
DraIII CAC_nnn'GTG
Cuts at: 342 342
Size: 4073
DrdI GACnn_nn'nnGTC
Cuts at: 1779 2637 1779
Size: 858 3215
EaeI y'GGCC_r
Cuts at: 621 1658 1832 2223 2250 3595 621
Size: 1037 174 391 27 1345 1099
Fragments arranged by size:
1345 1099 1037 391 174 27
EagI C'GGCC_G
Cuts at: 1658 1658
Size: 4073
EarI CTCTTCn'nnn_
Cuts at: 160 345 2096 2306 160
Size: 185 1751 210 1927
Fragments arranged by size:
1927 1751 210 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2281 2591 2737 3883 2281
Size: 310 146 1146 2471
Fragments arranged by size:
2471 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1085 1085
Size: 4073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 363 476 756 1898 2330 3077 3345 252
Size: 111 113 280 1142 432 747 268 980
Fragments arranged by size:
1142 980 747 432 280 268 113 111
EcoO109I rG'GnC_Cy
Cuts at: 1212 1212
Size: 4073
EcoRI G'AATT_C
Cuts at: 1077 1077
Size: 4073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 4073
FspI TGC'GCA
Cuts at: 1854 1854
Size: 4073
HaeII r_GCGC'y
Cuts at: 267 1507 1755 2777 3756 267
Size: 1240 248 1022 979 584
Fragments arranged by size:
1240 1022 979 584 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 1915 1945 1947 1977 562
Size: 32 1321 30 2 30 2658
Fragments arranged by size:
2658 1321 32 30 30 2
HincII GTy'rAC
Cuts at: 1112 1112
Size: 4073
KasI G'GCGC_C
Cuts at: 1503 1751 1503
Size: 248 3825
KpnI G_GTAC'C
Cuts at: 1093 1093
Size: 4073
MluI A'CGCG_T
Cuts at: 3506 3506
Size: 4073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2744 2928 2744
Size: 184 3889
MscI TGG'CCA
Cuts at: 623 1834 623
Size: 1211 2862
MslI CAynn'nnrTG
Cuts at: 629 2062 2189 629
Size: 1433 127 2513
Fragments arranged by size:
2513 1433 127
MspA1I CmG'CkG
Cuts at: 144 1184 1498 1858 2871 3116 144
Size: 1040 314 360 1013 245 1101
Fragments arranged by size:
1101 1040 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2254 2254
Size: 4073
NarI GG'CG_CC
Cuts at: 1504 1752 1504
Size: 248 3825
NdeI CA'TA_TG
Cuts at: 291 291
Size: 4073
NgoMIV G'CCGG_C
Cuts at: 2252 2252
Size: 4073
NspI r_CATG'y
Cuts at: 1126 1626 2533 1126
Size: 500 907 2666
Fragments arranged by size:
2666 907 500
PciI A'CATG_T
Cuts at: 2529 2529
Size: 4073
PmeI GTTT'AAAC
Cuts at: 1072 1072
Size: 4073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 4041
PstI C_TGCA'G
Cuts at: 1120 1805 1120
Size: 685 3388
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4073
PvuII CAG'CTG
Cuts at: 144 1498 1858 144
Size: 1354 360 2359
Fragments arranged by size:
2359 1354 360
RsrII CG'GwC_CG
Cuts at: 2268 2268
Size: 4073
SacI G_AGCT'C
Cuts at: 1087 1087
Size: 4073
SalI G'TCGA_C
Cuts at: 1110 1110
Size: 4073
SapI GCTCTTCn'nnn_
Cuts at: 160 2096 2306 160
Size: 1936 210 1927
Fragments arranged by size:
1936 1927 210
SbfI CC_TGCA'GG
Cuts at: 1120 1120
Size: 4073
ScaI AGT'ACT
Cuts at: 3590 3590
Size: 4073
SexAI A'CCwGG_T
Cuts at: 399 399
Size: 4073
SfcI C'TryA_G
Cuts at: 33 1116 1801 2794 2985 33
Size: 1083 685 993 191 1121
Fragments arranged by size:
1121 1083 993 685 191
SfoI GGC'GCC
Cuts at: 1505 1753 1505
Size: 248 3825
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
SmaI CCC'GGG
Cuts at: 1095 1095
Size: 4073
SmlI C'TyrA_G
Cuts at: 1228 2143 2635 2897 1228
Size: 915 492 262 2404
Fragments arranged by size:
2404 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4073
SphI G_CATG'C
Cuts at: 1126 1626 1126
Size: 500 3573
StyI C'CwwG_G
Cuts at: 1011 1207 1561 1011
Size: 196 354 3523
Fragments arranged by size:
3523 354 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 539 745 1656 1789 2248 2457 539
Size: 206 911 133 459 209 2155
Fragments arranged by size:
2155 911 459 209 206 133
TatI w'GTAC_w
Cuts at: 3588 3588
Size: 4073
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 341 373 3590 3622 341
Size: 32 3217 32 792
Fragments arranged by size:
3217 792 32 32
Tth111I GACn'n_nGTC
Cuts at: 1870 1870
Size: 4073
XbaI T'CTAG_A
Cuts at: 1104 1104
Size: 4073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 616 1018 1568 616
Size: 402 550 3121
Fragments arranged by size:
3121 550 402
XmaI C'CCGG_G
Cuts at: 1093 1093
Size: 4073
XmnI GAAnn'nnTTC
Cuts at: 154 699 154
Size: 545 3528
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI