Restriction map for
pF1KB8120
(Circular) (Six-base) MAPSORT of: pF1KB8120.seq Check: 1128 from: 1 to: 4568
pF1KB8120 4568 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 132 132
Size: 4568
AccI GT'mk_AC
Cuts at: 1606 1606
Size: 4568
Acc65I G'GTAC_C
Cuts at: 1584 1584
Size: 4568
AclI AA'CG_TT
Cuts at: 4408 4408
Size: 4568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 384 2393 2825 3572 3840 261
Size: 123 2009 432 747 268 989
Fragments arranged by size:
2009 989 747 432 268 123
AflIII A'CryG_T
Cuts at: 552 1920 3024 4001 552
Size: 1368 1104 977 1119
Fragments arranged by size:
1368 1119 1104 977
AgeI A'CCGG_T
Cuts at: 2956 2956
Size: 4568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1295 1295
Size: 4568
AlwNI CAG_nnn'CTG
Cuts at: 431 597 985 1077 1873 3440 431
Size: 166 388 92 796 1567 1559
Fragments arranged by size:
1567 1559 796 388 166 92
ApaI G_GGCC'C
Cuts at: 417 680 1249 417
Size: 263 569 3736
Fragments arranged by size:
3736 569 263
ApaLI G'TGCA_C
Cuts at: 601 1058 3338 601
Size: 457 2280 1831
Fragments arranged by size:
2280 1831 457
ApoI r'AATT_y
Cuts at: 1572 3921 4177 1572
Size: 2349 256 1963
Fragments arranged by size:
2349 1963 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4568
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
AvaI C'yCGr_G
Cuts at: 316 1588 316
Size: 1272 3296
BamHI G'GATC_C
Cuts at: 1593 1593
Size: 4568
BanI G'GyrC_C
Cuts at: 782 892 1584 1998 2246 2281 782
Size: 110 692 414 248 35 3069
Fragments arranged by size:
3069 692 414 248 110 35
BanII G_rGCy'C
Cuts at: 417 680 832 857 1249 1582 2612 417
Size: 263 152 25 392 333 1030 2373
Fragments arranged by size:
2373 1030 392 333 263 152 25
BbeI G_GCGC'C
Cuts at: 2002 2250 2002
Size: 248 4320
BciVI GTATCCnnnnn_n'
Cuts at: 2466 3227 4568 2466
Size: 761 1341 2466
Fragments arranged by size:
2466 1341 761
BclI T'GATC_A
Cuts at: 1163 1253 2088 1163
Size: 90 835 3643
Fragments arranged by size:
3643 835 90
BfrBI ATG'CAT
Cuts at: 693 693
Size: 4568
BglI GCCn_nnn'nGGC
Cuts at: 470 958 470
Size: 488 4080
BglII A'GATC_T
Cuts at: 2083 4120 2083
Size: 2037 2531
BlpI GC'TnA_GC
Cuts at: 826 1680 826
Size: 854 3714
Bme1580I G_kGCm'C
Cuts at: 417 571 605 680 816 954 1062 1249 2193
Size: 154 34 75 136 138 108 187 944
Cuts at: 2193 2286 3342 417
Size: 93 1056 1643
Fragments arranged by size:
1643 1056 944 187 154 138 136 108
93 75 34
BmrI ACTGGGnnnn_n'
Cuts at: 1780 1861 2196 4340 1780
Size: 81 335 2144 2008
Fragments arranged by size:
2144 2008 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 242 2935 242
Size: 2693 1875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 534 607 754 1477 250
Size: 284 73 147 723 3341
Fragments arranged by size:
3341 723 284 147 73
Bpu10I CC'TnA_GC
Cuts at: 191 749 191
Size: 558 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 271 1744 2623 3115 3413 187
Size: 84 1473 879 492 298 1342
Fragments arranged by size:
1473 1342 879 492 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 458 487 4272 458
Size: 29 3785 754
Fragments arranged by size:
3785 754 29
BsaAI yAC'GTr
Cuts at: 606 1011 1921 606
Size: 405 910 3253
Fragments arranged by size:
3253 910 405
BsaBI GATnn'nnATC
Cuts at: 6 2082 4119 6
Size: 2076 2037 455
Fragments arranged by size:
2076 2037 455
BsaHI Gr'CG_yC
Cuts at: 1999 2247 2949 4445 1999
Size: 248 702 1496 2122
Fragments arranged by size:
2122 1496 702 248
BsaWI w'CCGG_w
Cuts at: 215 1358 1757 2278 2956 3230 3377 3788 215
Size: 1143 399 521 678 274 147 411 995
Fragments arranged by size:
1143 995 678 521 411 399 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 336 742 908 1231 190
Size: 146 406 166 323 3527
Fragments arranged by size:
3527 406 323 166 146
BseYI C'CCAG_C
Cuts at: 872 1410 1993 2751 3328 3824 872
Size: 538 583 758 577 496 1616
Fragments arranged by size:
1616 758 583 577 538 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 919 998 1461 199
Size: 720 79 463 3306
Fragments arranged by size:
3306 720 463 79
BsiEI CG_ry'CG
Cuts at: 104 2110 2156 3364 104
Size: 2006 46 1208 1308
Fragments arranged by size:
2006 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 520 605 857 1062 1071 1582 2360 2550 3342
Size: 85 252 205 9 511 778 190 792
Cuts at: 3342 520
Size: 1746
Fragments arranged by size:
1746 792 778 511 252 205 190 85
9
BsmI GAATG_Cn'
Cuts at: 253 253
Size: 4568
Bsp1286I G_dGCh'C
Cuts at: 417 520 571 605 680 816 832 857 954
Size: 103 51 34 75 136 16 25 97
Cuts at: 954 1062 1071 1249 1582 2193 2286 2360 2550
Size: 108 9 178 333 611 93 74 190
Cuts at: 2550 2612 3342 417
Size: 62 730 1643
Fragments arranged by size:
1643 730 611 333 190 178 136 108
103 97 93 75 74 62 51 34
25 16 9
BspEI T'CCGG_A
Cuts at: 1358 3788 1358
Size: 2430 2138
BspHI T'CATG_A
Cuts at: 332 1160 3744 332
Size: 828 2584 1156
Fragments arranged by size:
2584 1156 828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 132 1618 1832 2134 2515 132
Size: 1486 214 302 381 2185
Fragments arranged by size:
2185 1486 381 302 214
BsrBI CCG'CTC
Cuts at: 50 113 612 780 2860 2914 4398 4563 50
Size: 63 499 168 2080 54 1484 165 55
Fragments arranged by size:
2080 1484 499 168 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 399 2480 399
Size: 2081 2487
BsrFI r'CCGG_y
Cuts at: 1226 2566 2747 2956 1226
Size: 1340 181 209 2838
Fragments arranged by size:
2838 1340 209 181
BssSI C'ACGA_G
Cuts at: 1113 2839 3197 1113
Size: 1726 358 2484
Fragments arranged by size:
2484 1726 358
BstAPI GCAn_nnn'nTGC
Cuts at: 600 1945 600
Size: 1345 3223
BstBI TT'CG_AA
Cuts at: 2929 2929
Size: 4568
BstXI CCAn_nnnn'nTGG
Cuts at: 144 1203 144
Size: 1059 3509
BstYI r'GATC_y
Cuts at: 1 509 1103 1347 1593 2060 2083 2418 2664
Size: 508 594 244 246 467 23 335 246
Cuts at: 2664 3676 3762 3774 4120 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 594 508 467 449 346 335 246
246 244 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 576 576
Size: 4568
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2518 2699 2862 2518
Size: 181 163 4224
Fragments arranged by size:
4224 181 163
BtsI GCAGTG_nn'
Cuts at: 1075 1811 1075
Size: 736 3832
DraI TTT'AAA
Cuts at: 1567 1567
Size: 4568
DrdI GACnn_nn'nnGTC
Cuts at: 2274 3132 2274
Size: 858 3710
EaeI y'GGCC_r
Cuts at: 146 638 995 1319 1448 2153 2327 2718 2745
Size: 492 357 324 129 705 174 391 27
Cuts at: 2745 4090 146
Size: 1345 624
Fragments arranged by size:
1345 705 624 492 391 357 324 174
129 27
EagI C'GGCC_G
Cuts at: 2153 2153
Size: 4568
EarI CTCTTCn'nnn_
Cuts at: 1034 1513 2591 2801 1034
Size: 479 1078 210 2801
Fragments arranged by size:
2801 1078 479 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1116 2776 3086 3232 4378 1116
Size: 1660 310 146 1146 1306
Fragments arranged by size:
1660 1306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 855 1580 855
Size: 725 3843
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 261 384 534 607 754 1477 2393 2825
Size: 11 123 150 73 147 723 916 432
Cuts at: 2825 3572 3840 250
Size: 747 268 978
Fragments arranged by size:
978 916 747 723 432 268 150 147
123 73 11
EcoNI CCTnn'n_nnAGG
Cuts at: 265 575 752 1510 265
Size: 310 177 758 3323
Fragments arranged by size:
3323 758 310 177
EcoO109I rG'GnC_Cy
Cuts at: 413 757 982 1245 1246 1302 1335 1707 413
Size: 344 225 263 1 56 33 372 3274
Fragments arranged by size:
3274 372 344 263 225 56 33 1
EcoRI G'AATT_C
Cuts at: 1572 1572
Size: 4568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1536 2587 1536
Size: 1051 3517
FspI TGC'GCA
Cuts at: 1015 2349 1015
Size: 1334 3234
FspAI rTGC'GCAy
Cuts at: 1015 1015
Size: 4568
HaeII r_GCGC'y
Cuts at: 2002 2250 3272 4251 2002
Size: 248 1022 979 2319
Fragments arranged by size:
2319 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1033 1065 2410 2440 2442 2472 1033
Size: 32 1345 30 2 30 3129
Fragments arranged by size:
3129 1345 32 30 30 2
HincII GTy'rAC
Cuts at: 1607 1607
Size: 4568
KasI G'GCGC_C
Cuts at: 1998 2246 1998
Size: 248 4320
KpnI G_GTAC'C
Cuts at: 1588 1588
Size: 4568
MluI A'CGCG_T
Cuts at: 552 4001 552
Size: 3449 1119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3239 3423 3239
Size: 184 4384
MscI TGG'CCA
Cuts at: 148 640 997 1321 1450 2329 148
Size: 492 357 324 129 879 2387
Fragments arranged by size:
2387 879 492 357 324 129
MslI CAynn'nnrTG
Cuts at: 142 688 2557 2684 142
Size: 546 1869 127 2026
Fragments arranged by size:
2026 1869 546 127
MspA1I CmG'CkG
Cuts at: 597 941 1679 1993 2353 3366 3611 597
Size: 344 738 314 360 1013 245 1554
Fragments arranged by size:
1554 1013 738 360 344 314 245
NaeI GCC'GGC
Cuts at: 2749 2749
Size: 4568
NarI GG'CG_CC
Cuts at: 1999 2247 1999
Size: 248 4320
NgoMIV G'CCGG_C
Cuts at: 2747 2747
Size: 4568
NsiI A_TGCA'T
Cuts at: 695 695
Size: 4568
NspI r_CATG'y
Cuts at: 1621 2121 3028 1621
Size: 500 907 3161
Fragments arranged by size:
3161 907 500
PciI A'CATG_T
Cuts at: 3024 3024
Size: 4568
PflMI CCAn_nnn'nTGG
Cuts at: 240 240
Size: 4568
PfoI T'CCnGG_A
Cuts at: 722 1463 722
Size: 741 3827
PmeI GTTT'AAAC
Cuts at: 1567 1567
Size: 4568
PmlI CAC'GTG
Cuts at: 606 1011 606
Size: 405 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4536
PpuMI rG'GwC_Cy
Cuts at: 1335 1335
Size: 4568
PspOMI G'GGCC_C
Cuts at: 413 676 1245 413
Size: 263 569 3736
Fragments arranged by size:
3736 569 263
PstI C_TGCA'G
Cuts at: 653 959 1346 1615 2300 653
Size: 306 387 269 685 2921
Fragments arranged by size:
2921 685 387 306 269
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4568
PvuII CAG'CTG
Cuts at: 597 941 1993 2353 597
Size: 344 1052 360 2812
Fragments arranged by size:
2812 1052 360 344
RsrII CG'GwC_CG
Cuts at: 2763 2763
Size: 4568
SacI G_AGCT'C
Cuts at: 857 1582 857
Size: 725 3843
SalI G'TCGA_C
Cuts at: 1605 1605
Size: 4568
SapI GCTCTTCn'nnn_
Cuts at: 2591 2801 2591
Size: 210 4358
SbfI CC_TGCA'GG
Cuts at: 959 1346 1615 959
Size: 387 269 3912
Fragments arranged by size:
3912 387 269
ScaI AGT'ACT
Cuts at: 328 4085 328
Size: 3757 811
SexAI A'CCwGG_T
Cuts at: 520 520
Size: 4568
SfcI C'TryA_G
Cuts at: 33 368 649 955 1342 1611 2296 3289 3480
Size: 335 281 306 387 269 685 993 191
Cuts at: 3480 33
Size: 1121
Fragments arranged by size:
1121 993 685 387 335 306 281 269
191
SfoI GGC'GCC
Cuts at: 2000 2248 2000
Size: 248 4320
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
SmaI CCC'GGG
Cuts at: 1590 1590
Size: 4568
SmlI C'TyrA_G
Cuts at: 202 286 1723 2638 3130 3392 202
Size: 84 1437 915 492 262 1378
Fragments arranged by size:
1437 1378 915 492 262 84
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4568
SphI G_CATG'C
Cuts at: 1621 2121 1621
Size: 500 4068
SspI AAT'ATT
Cuts at: 309 309
Size: 4568
StuI AGG'CCT
Cuts at: 961 961
Size: 4568
StyI C'CwwG_G
Cuts at: 833 1702 2056 833
Size: 869 354 3345
Fragments arranged by size:
3345 869 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 440 780 877 886 2151 2284 2743 2952 440
Size: 340 97 9 1265 133 459 209 2056
Fragments arranged by size:
2056 1265 459 340 209 133 97 9
TatI w'GTAC_w
Cuts at: 326 4083 326
Size: 3757 811
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4085 4117 4085
Size: 32 4536
Tth111I GACn'n_nGTC
Cuts at: 2365 2365
Size: 4568
XbaI T'CTAG_A
Cuts at: 1599 1599
Size: 4568
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 385 840 2063 385
Size: 455 1223 2890
Fragments arranged by size:
2890 1223 455
XmaI C'CCGG_G
Cuts at: 1588 1588
Size: 4568
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I BtgI ClaI CspCI
DraIII EcoRV FseI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI