Restriction map for
pF1KB8117
(Circular) (Six-base) MAPSORT of: pF1KB8117.seq Check: 7885 from: 1 to: 4586
pF1KB8117 4586 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 284 1035 1069 284
Size: 751 34 3801
Fragments arranged by size:
3801 751 34
AccI GT'mk_AC
Cuts at: 1208 1624 1208
Size: 416 4170
Acc65I G'GTAC_C
Cuts at: 1602 1602
Size: 4586
AclI AA'CG_TT
Cuts at: 4426 4426
Size: 4586
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2411 2843 3590 3858 1402
Size: 1009 432 747 268 2130
Fragments arranged by size:
2130 1009 747 432 268
AflIII A'CryG_T
Cuts at: 1938 3042 4019 1938
Size: 1104 977 2505
Fragments arranged by size:
2505 1104 977
AgeI A'CCGG_T
Cuts at: 2974 2974
Size: 4586
AleI CACnn'nnGTG
Cuts at: 643 643
Size: 4586
AlwNI CAG_nnn'CTG
Cuts at: 615 1050 1298 1368 1380 1437 1891 3458 615
Size: 435 248 70 12 57 454 1567 1743
Fragments arranged by size:
1743 1567 454 435 248 70 57 12
ApaI G_GGCC'C
Cuts at: 528 528
Size: 4586
ApaLI G'TGCA_C
Cuts at: 3356 3356
Size: 4586
ApoI r'AATT_y
Cuts at: 533 1590 3939 4195 533
Size: 1057 2349 256 924
Fragments arranged by size:
2349 1057 924 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4586
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
AvaI C'yCGr_G
Cuts at: 183 367 569 921 1508 1606 183
Size: 184 202 352 587 98 3163
Fragments arranged by size:
3163 587 352 202 184 98
BamHI G'GATC_C
Cuts at: 870 1548 1611 870
Size: 678 63 3845
Fragments arranged by size:
3845 678 63
BanI G'GyrC_C
Cuts at: 354 462 1602 2016 2264 2299 354
Size: 108 1140 414 248 35 2641
Fragments arranged by size:
2641 1140 414 248 108 35
BanII G_rGCy'C
Cuts at: 528 858 1600 2630 528
Size: 330 742 1030 2484
Fragments arranged by size:
2484 1030 742 330
BbeI G_GCGC'C
Cuts at: 358 2020 2268 358
Size: 1662 248 2676
Fragments arranged by size:
2676 1662 248
BbsI GAAGACnn'nnnn_
Cuts at: 223 775 1160 223
Size: 552 385 3649
Fragments arranged by size:
3649 552 385
BciVI GTATCCnnnnn_n'
Cuts at: 2484 3245 4586 2484
Size: 761 1341 2484
Fragments arranged by size:
2484 1341 761
BclI T'GATC_A
Cuts at: 360 2106 360
Size: 1746 2840
BglI GCCn_nnn'nGGC
Cuts at: 254 994 1432 1574 254
Size: 740 438 142 3266
Fragments arranged by size:
3266 740 438 142
BglII A'GATC_T
Cuts at: 227 2101 4138 227
Size: 1874 2037 675
Fragments arranged by size:
2037 1874 675
BlpI GC'TnA_GC
Cuts at: 499 1177 1441 1698 499
Size: 678 264 257 3387
Fragments arranged by size:
3387 678 264 257
Bme1580I G_kGCm'C
Cuts at: 329 528 1139 1223 1522 2211 2304 3360 329
Size: 199 611 84 299 689 93 1056 1555
Fragments arranged by size:
1555 1056 689 611 299 199 93 84
BmgBI CAC'GTC
Cuts at: 456 678 456
Size: 222 4364
BmrI ACTGGGnnnn_n'
Cuts at: 1488 1798 1879 2214 4358 1488
Size: 310 81 335 2144 1716
Fragments arranged by size:
2144 1716 335 310 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 4586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 733 877 1126 637
Size: 96 144 249 4097
Fragments arranged by size:
4097 249 144 96
Bpu10I CC'TnA_GC
Cuts at: 628 704 628
Size: 76 4510
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1762 2641 3133 3431 1762
Size: 879 492 298 2917
Fragments arranged by size:
2917 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 895 4290 895
Size: 3395 1191
BsaAI yAC'GTr
Cuts at: 300 1939 300
Size: 1639 2947
BsaBI GATnn'nnATC
Cuts at: 6 2100 4137 6
Size: 2094 2037 455
Fragments arranged by size:
2094 2037 455
BsaHI Gr'CG_yC
Cuts at: 355 2017 2265 2967 4463 355
Size: 1662 248 702 1496 478
Fragments arranged by size:
1662 1496 702 478 248
BsaWI w'CCGG_w
Cuts at: 1775 2296 2974 3248 3395 3806 1775
Size: 521 678 274 147 411 2555
Fragments arranged by size:
2555 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 187 217 187
Size: 30 4556
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 1513 559
Size: 954 3632
BseYI C'CCAG_C
Cuts at: 249 1040 1298 1387 2011 2769 3346 3842 249
Size: 791 258 89 624 758 577 496 993
Fragments arranged by size:
993 791 758 624 577 496 258 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1151 1151
Size: 4586
BsiEI CG_ry'CG
Cuts at: 104 2128 2174 3382 104
Size: 2024 46 1208 1308
Fragments arranged by size:
2024 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 181 565 710 1178 1600 2378 2568 3360 181
Size: 384 145 468 422 778 190 792 1407
Fragments arranged by size:
1407 792 778 468 422 384 190 145
Bsp1286I G_dGCh'C
Cuts at: 181 329 528 565 710 858 1139 1178 1223
Size: 148 199 37 145 148 281 39 45
Cuts at: 1223 1522 1600 2211 2304 2378 2568 2630 3360
Size: 299 78 611 93 74 190 62 730
Cuts at: 3360 181
Size: 1407
Fragments arranged by size:
1407 730 611 299 281 199 190 148
148 145 93 78 74 62 45 39
37
BspEI T'CCGG_A
Cuts at: 3806 3806
Size: 4586
BspHI T'CATG_A
Cuts at: 3762 3762
Size: 4586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 284 1035 1069 1636 1850 2152 2533 284
Size: 751 34 567 214 302 381 2337
Fragments arranged by size:
2337 751 567 381 302 214 34
BsrBI CCG'CTC
Cuts at: 50 2878 2932 4416 4581 50
Size: 2828 54 1484 165 55
Fragments arranged by size:
2828 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2498 2498
Size: 4586
BsrFI r'CCGG_y
Cuts at: 788 975 1357 2584 2765 2974 788
Size: 187 382 1227 181 209 2400
Fragments arranged by size:
2400 1227 382 209 187 181
BssSI C'ACGA_G
Cuts at: 2857 3215 2857
Size: 358 4228
BstAPI GCAn_nnn'nTGC
Cuts at: 1050 1963 1050
Size: 913 3673
BstBI TT'CG_AA
Cuts at: 951 2947 951
Size: 1996 2590
BstYI r'GATC_y
Cuts at: 1 227 870 1548 1611 2078 2101 2436 2682
Size: 226 643 678 63 467 23 335 246
Cuts at: 2682 3694 3780 3792 4138 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 678 643 467 449 346 335 246
226 86 63 23 12
Bsu36I CC'TnA_GG
Cuts at: 1553 1553
Size: 4586
BtgI C'CryG_G
Cuts at: 107 1139 107
Size: 1032 3554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2536 2717 2880 2536
Size: 181 163 4242
Fragments arranged by size:
4242 181 163
BtsI GCAGTG_nn'
Cuts at: 249 1139 1829 249
Size: 890 690 3006
Fragments arranged by size:
3006 890 690
DraI TTT'AAA
Cuts at: 1585 1585
Size: 4586
DraIII CAC_nnn'GTG
Cuts at: 492 492
Size: 4586
DrdI GACnn_nn'nnGTC
Cuts at: 2292 3150 2292
Size: 858 3728
EaeI y'GGCC_r
Cuts at: 414 956 1359 1459 2171 2345 2736 2763 4108
Size: 542 403 100 712 174 391 27 1345
Cuts at: 4108 414
Size: 892
Fragments arranged by size:
1345 892 712 542 403 391 174 100
27
EagI C'GGCC_G
Cuts at: 2171 2171
Size: 4586
EarI CTCTTCn'nnn_
Cuts at: 1300 2609 2819 1300
Size: 1309 210 3067
Fragments arranged by size:
3067 1309 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 555 994 1075 2794 3104 3250 4396 555
Size: 439 81 1719 310 146 1146 745
Fragments arranged by size:
1719 1146 745 439 310 146 81
EcoICRI GAG'CTC
Cuts at: 1598 1598
Size: 4586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 733 877 1126 1402 2411 2843 3590 3858
Size: 96 144 249 276 1009 432 747 268
Cuts at: 3858 637
Size: 1365
Fragments arranged by size:
1365 1009 747 432 276 268 249 144
96
EcoO109I rG'GnC_Cy
Cuts at: 525 912 1017 1312 1377 1434 1725 525
Size: 387 105 295 65 57 291 3386
Fragments arranged by size:
3386 387 295 291 105 65 57
EcoRI G'AATT_C
Cuts at: 1590 1590
Size: 4586
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4586
FspI TGC'GCA
Cuts at: 2367 2367
Size: 4586
HaeII r_GCGC'y
Cuts at: 129 358 2020 2268 3290 4269 129
Size: 229 1662 248 1022 979 446
Fragments arranged by size:
1662 1022 979 446 248 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 744 776 2428 2458 2460 2490 744
Size: 32 1652 30 2 30 2840
Fragments arranged by size:
2840 1652 32 30 30 2
HincII GTy'rAC
Cuts at: 1209 1625 1209
Size: 416 4170
HindIII A'AGCT_T
Cuts at: 1234 1234
Size: 4586
KasI G'GCGC_C
Cuts at: 354 2016 2264 354
Size: 1662 248 2676
Fragments arranged by size:
2676 1662 248
KpnI G_GTAC'C
Cuts at: 1606 1606
Size: 4586
MluI A'CGCG_T
Cuts at: 4019 4019
Size: 4586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3257 3441 3257
Size: 184 4402
MscI TGG'CCA
Cuts at: 2347 2347
Size: 4586
MslI CAynn'nnrTG
Cuts at: 643 1336 2575 2702 643
Size: 693 1239 127 2527
Fragments arranged by size:
2527 1239 693 127
MspA1I CmG'CkG
Cuts at: 253 419 1295 1697 2011 2371 3384 3629 253
Size: 166 876 402 314 360 1013 245 1210
Fragments arranged by size:
1210 1013 876 402 360 314 245 166
NaeI GCC'GGC
Cuts at: 790 977 2767 790
Size: 187 1790 2609
Fragments arranged by size:
2609 1790 187
NarI GG'CG_CC
Cuts at: 355 2017 2265 355
Size: 1662 248 2676
Fragments arranged by size:
2676 1662 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4586
NgoMIV G'CCGG_C
Cuts at: 788 975 2765 788
Size: 187 1790 2609
Fragments arranged by size:
2609 1790 187
NspI r_CATG'y
Cuts at: 1639 2139 3046 1639
Size: 500 907 3179
Fragments arranged by size:
3179 907 500
PasI CC'CwG_GG
Cuts at: 741 1532 741
Size: 791 3795
PciI A'CATG_T
Cuts at: 3042 3042
Size: 4586
PflMI CCAn_nnn'nTGG
Cuts at: 492 615 492
Size: 123 4463
PfoI T'CCnGG_A
Cuts at: 893 893
Size: 4586
PmeI GTTT'AAAC
Cuts at: 1585 1585
Size: 4586
PmlI CAC'GTG
Cuts at: 300 300
Size: 4586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 3725
Size: 32 4554
PpuMI rG'GwC_Cy
Cuts at: 912 1312 1377 912
Size: 400 65 4121
Fragments arranged by size:
4121 400 65
PspOMI G'GGCC_C
Cuts at: 524 524
Size: 4586
PstI C_TGCA'G
Cuts at: 1294 1376 1633 2318 1294
Size: 82 257 685 3562
Fragments arranged by size:
3562 685 257 82
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4586
PvuII CAG'CTG
Cuts at: 253 2011 2371 253
Size: 1758 360 2468
Fragments arranged by size:
2468 1758 360
RsrII CG'GwC_CG
Cuts at: 2781 2781
Size: 4586
SacI G_AGCT'C
Cuts at: 1600 1600
Size: 4586
SalI G'TCGA_C
Cuts at: 1207 1623 1207
Size: 416 4170
SapI GCTCTTCn'nnn_
Cuts at: 2609 2819 2609
Size: 210 4376
SbfI CC_TGCA'GG
Cuts at: 1633 1633
Size: 4586
ScaI AGT'ACT
Cuts at: 4103 4103
Size: 4586
SfcI C'TryA_G
Cuts at: 33 1290 1316 1372 1629 2314 3307 3498 33
Size: 1257 26 56 257 685 993 191 1121
Fragments arranged by size:
1257 1121 993 685 257 191 56 26
SfoI GGC'GCC
Cuts at: 356 2018 2266 356
Size: 1662 248 2676
Fragments arranged by size:
2676 1662 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
SmaI CCC'GGG
Cuts at: 571 1510 1608 571
Size: 939 98 3549
Fragments arranged by size:
3549 939 98
SmlI C'TyrA_G
Cuts at: 1741 2656 3148 3410 1741
Size: 915 492 262 2917
Fragments arranged by size:
2917 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4586
SphI G_CATG'C
Cuts at: 1639 2139 1639
Size: 500 4086
SrfI GCCC'GGGC
Cuts at: 571 571
Size: 4586
StuI AGG'CCT
Cuts at: 481 1283 1413 481
Size: 802 130 3654
Fragments arranged by size:
3654 802 130
StyI C'CwwG_G
Cuts at: 107 1720 2074 107
Size: 1613 354 2619
Fragments arranged by size:
2619 1613 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 733 1290 2169 2302 2761 2970 733
Size: 557 879 133 459 209 2349
Fragments arranged by size:
2349 879 557 459 209 133
TatI w'GTAC_w
Cuts at: 747 4101 747
Size: 3354 1232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 187 219 4103 4135 187
Size: 32 3884 32 638
Fragments arranged by size:
3884 638 32 32
Tth111I GACn'n_nGTC
Cuts at: 2383 2383
Size: 4586
XbaI T'CTAG_A
Cuts at: 1617 1617
Size: 4586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1146 1539 2081 1146
Size: 393 542 3651
Fragments arranged by size:
3651 542 393
XmaI C'CCGG_G
Cuts at: 569 1508 1606 569
Size: 939 98 3549
Fragments arranged by size:
3549 939 98
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SspI SwaI XhoI
XmnI ZraI