Restriction map for
pF1KB8110
(Circular) (Six-base) MAPSORT of: pF1KB8110.seq Check: 7489 from: 1 to: 3653
pF1KB8110 3653 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 691 691
Size: 3653
Acc65I G'GTAC_C
Cuts at: 669 669
Size: 3653
AclI AA'CG_TT
Cuts at: 3493 3493
Size: 3653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 1478 1910 2657 2925 279
Size: 1199 432 747 268 1007
Fragments arranged by size:
1199 1007 747 432 268
AflIII A'CryG_T
Cuts at: 1005 2109 3086 1005
Size: 1104 977 1572
Fragments arranged by size:
1572 1104 977
AgeI A'CCGG_T
Cuts at: 2041 2041
Size: 3653
AlwNI CAG_nnn'CTG
Cuts at: 248 958 2525 248
Size: 710 1567 1376
Fragments arranged by size:
1567 1376 710
ApaI G_GGCC'C
Cuts at: 618 618
Size: 3653
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 657 3006 3262 657
Size: 2349 256 1048
Fragments arranged by size:
2349 1048 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 402 598 673 402
Size: 196 75 3382
Fragments arranged by size:
3382 196 75
AvrII C'CTAG_G
Cuts at: 282 282
Size: 3653
BamHI G'GATC_C
Cuts at: 532 678 532
Size: 146 3507
BanI G'GyrC_C
Cuts at: 417 544 669 1083 1331 1366 417
Size: 127 125 414 248 35 2704
Fragments arranged by size:
2704 414 248 127 125 35
BanII G_rGCy'C
Cuts at: 599 618 667 1697 599
Size: 19 49 1030 2555
Fragments arranged by size:
2555 1030 49 19
BbeI G_GCGC'C
Cuts at: 1087 1335 1087
Size: 248 3405
BbvCI CC'TCA_GC
Cuts at: 641 641
Size: 3653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3619
BciVI GTATCCnnnnn_n'
Cuts at: 1551 2312 3653 1551
Size: 761 1341 1551
Fragments arranged by size:
1551 1341 761
BclI T'GATC_A
Cuts at: 1173 1173
Size: 3653
BglI GCCn_nnn'nGGC
Cuts at: 245 245
Size: 3653
BglII A'GATC_T
Cuts at: 1168 3205 1168
Size: 2037 1616
BlpI GC'TnA_GC
Cuts at: 499 765 499
Size: 266 3387
Bme1580I G_kGCm'C
Cuts at: 403 422 618 1278 1371 2427 403
Size: 19 196 660 93 1056 1629
Fragments arranged by size:
1629 1056 660 196 93 19
BmrI ACTGGGnnnn_n'
Cuts at: 363 865 946 1281 3425 363
Size: 502 81 335 2144 591
Fragments arranged by size:
2144 591 502 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 3653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 415
Size: 3653
Bpu10I CC'TnA_GC
Cuts at: 593 641 593
Size: 48 3605
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1708 2200 2498 829
Size: 879 492 298 1984
Fragments arranged by size:
1984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3357 3357
Size: 3653
BsaAI yAC'GTr
Cuts at: 1006 1006
Size: 3653
BsaBI GATnn'nnATC
Cuts at: 6 1167 3204 6
Size: 1161 2037 455
Fragments arranged by size:
2037 1161 455
BsaHI Gr'CG_yC
Cuts at: 1084 1332 2034 3530 1084
Size: 248 702 1496 1207
Fragments arranged by size:
1496 1207 702 248
BsaWI w'CCGG_w
Cuts at: 842 1363 2041 2315 2462 2873 842
Size: 521 678 274 147 411 1622
Fragments arranged by size:
1622 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 337 367 337
Size: 30 3623
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 427
Size: 3653
BseYI C'CCAG_C
Cuts at: 536 1078 1836 2413 2909 536
Size: 542 758 577 496 1280
Fragments arranged by size:
1280 758 577 542 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 532 436
Size: 96 3557
BsiEI CG_ry'CG
Cuts at: 104 1195 1241 2449 104
Size: 1091 46 1208 1308
Fragments arranged by size:
1308 1208 1091 46
BsiHKAI G_wGCw'C
Cuts at: 667 1445 1635 2427 667
Size: 778 190 792 1893
Fragments arranged by size:
1893 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 403 422 599 618 667 1278 1371 1445 1635
Size: 19 177 19 49 611 93 74 190
Cuts at: 1635 1697 2427 403
Size: 62 730 1629
Fragments arranged by size:
1629 730 611 190 177 93 74 62
49 19 19
BspEI T'CCGG_A
Cuts at: 2873 2873
Size: 3653
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 703 917 1219 1600 703
Size: 214 302 381 2756
Fragments arranged by size:
2756 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1945 1999 3483 3648 50
Size: 1895 54 1484 165 55
Fragments arranged by size:
1895 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1565 1565
Size: 3653
BsrFI r'CCGG_y
Cuts at: 1651 1832 2041 1651
Size: 181 209 3263
Fragments arranged by size:
3263 209 181
BssSI C'ACGA_G
Cuts at: 409 490 1924 2282 409
Size: 81 1434 358 1780
Fragments arranged by size:
1780 1434 358 81
BstAPI GCAn_nnn'nTGC
Cuts at: 1030 1030
Size: 3653
BstBI TT'CG_AA
Cuts at: 2014 2014
Size: 3653
BstXI CCAn_nnnn'nTGG
Cuts at: 150 150
Size: 3653
BstYI r'GATC_y
Cuts at: 1 446 532 678 1145 1168 1503 1749 2761
Size: 445 86 146 467 23 335 246 1012
Cuts at: 2761 2847 2859 3205 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 445 346 335 246 146
86 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1603 1784 1947 1603
Size: 181 163 3309
Fragments arranged by size:
3309 181 163
BtsI GCAGTG_nn'
Cuts at: 896 896
Size: 3653
DraI TTT'AAA
Cuts at: 652 652
Size: 3653
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2217 1359
Size: 858 2795
EaeI y'GGCC_r
Cuts at: 1238 1412 1803 1830 3175 1238
Size: 174 391 27 1345 1716
Fragments arranged by size:
1716 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 626 1676 1886 626
Size: 1050 210 2393
Fragments arranged by size:
2393 1050 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1861 2171 2317 3463 1861
Size: 310 146 1146 2051
Fragments arranged by size:
2051 1146 310 146
EcoICRI GAG'CTC
Cuts at: 665 665
Size: 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 415 1478 1910 2657 2925 279
Size: 136 1063 432 747 268 1007
Fragments arranged by size:
1063 1007 747 432 268 136
EcoO109I rG'GnC_Cy
Cuts at: 117 603 614 615 792 117
Size: 486 11 1 177 2978
Fragments arranged by size:
2978 486 177 11 1
EcoRI G'AATT_C
Cuts at: 657 657
Size: 3653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1672 1672
Size: 3653
FspI TGC'GCA
Cuts at: 1434 1434
Size: 3653
HaeII r_GCGC'y
Cuts at: 1087 1335 2357 3336 1087
Size: 248 1022 979 1404
Fragments arranged by size:
1404 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 244 276 1495 1525 1527 1557 244
Size: 32 1219 30 2 30 2340
Fragments arranged by size:
2340 1219 32 30 30 2
HincII GTy'rAC
Cuts at: 692 692
Size: 3653
KasI G'GCGC_C
Cuts at: 1083 1331 1083
Size: 248 3405
KpnI G_GTAC'C
Cuts at: 673 673
Size: 3653
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2508 2324
Size: 184 3469
MscI TGG'CCA
Cuts at: 1414 1414
Size: 3653
MslI CAynn'nnrTG
Cuts at: 1642 1769 1642
Size: 127 3526
MspA1I CmG'CkG
Cuts at: 245 764 1078 1438 2451 2696 245
Size: 519 314 360 1013 245 1202
Fragments arranged by size:
1202 1013 519 360 314 245
NaeI GCC'GGC
Cuts at: 1834 1834
Size: 3653
NarI GG'CG_CC
Cuts at: 1084 1332 1084
Size: 248 3405
NgoMIV G'CCGG_C
Cuts at: 1832 1832
Size: 3653
NspI r_CATG'y
Cuts at: 363 706 1206 2113 363
Size: 343 500 907 1903
Fragments arranged by size:
1903 907 500 343
PasI CC'CwG_GG
Cuts at: 422 610 422
Size: 188 3465
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PflMI CCAn_nnn'nTGG
Cuts at: 234 234
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 3621
PpuMI rG'GwC_Cy
Cuts at: 117 603 117
Size: 486 3167
PspOMI G'GGCC_C
Cuts at: 614 614
Size: 3653
PstI C_TGCA'G
Cuts at: 700 1385 700
Size: 685 2968
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3653
PvuII CAG'CTG
Cuts at: 1078 1438 1078
Size: 360 3293
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 667 667
Size: 3653
SalI G'TCGA_C
Cuts at: 690 690
Size: 3653
SapI GCTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
SbfI CC_TGCA'GG
Cuts at: 700 700
Size: 3653
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SfcI C'TryA_G
Cuts at: 33 300 456 696 1381 2374 2565 33
Size: 267 156 240 685 993 191 1121
Fragments arranged by size:
1121 993 685 267 240 191 156
SfoI GGC'GCC
Cuts at: 1085 1333 1085
Size: 248 3405
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 404 675 404
Size: 271 3382
SmlI C'TyrA_G
Cuts at: 808 1723 2215 2477 808
Size: 915 492 262 1984
Fragments arranged by size:
1984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 363 706 1206 363
Size: 343 500 2810
Fragments arranged by size:
2810 500 343
SrfI GCCC'GGGC
Cuts at: 404 404
Size: 3653
StuI AGG'CCT
Cuts at: 469 469
Size: 3653
StyI C'CwwG_G
Cuts at: 282 787 1141 282
Size: 505 354 2794
Fragments arranged by size:
2794 505 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 180 241 562 1236 1369 1828 2037 180
Size: 61 321 674 133 459 209 1796
Fragments arranged by size:
1796 674 459 321 209 133 61
TatI w'GTAC_w
Cuts at: 3168 3168
Size: 3653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 337 369 3170 3202 337
Size: 32 2801 32 788
Fragments arranged by size:
2801 788 32 32
Tth111I GACn'n_nGTC
Cuts at: 1450 1450
Size: 3653
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 174 336 574 1148 174
Size: 162 238 574 2679
Fragments arranged by size:
2679 574 238 162
XmaI C'CCGG_G
Cuts at: 402 673 402
Size: 271 3382
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SspI SwaI XhoI XmnI
ZraI