Restriction map for
pF1KB8106
(Circular) (Six-base) MAPSORT of: pF1KB8106.seq Check: 6825 from: 1 to: 3644
pF1KB8106 3644 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 519 519
Size: 3644
AccI GT'mk_AC
Cuts at: 139 311 682 139
Size: 172 371 3101
Fragments arranged by size:
3101 371 172
Acc65I G'GTAC_C
Cuts at: 660 660
Size: 3644
AclI AA'CG_TT
Cuts at: 3484 3484
Size: 3644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1469 1901 2648 2916 1469
Size: 432 747 268 2197
Fragments arranged by size:
2197 747 432 268
AflIII A'CryG_T
Cuts at: 996 2100 3077 996
Size: 1104 977 1563
Fragments arranged by size:
1563 1104 977
AgeI A'CCGG_T
Cuts at: 2032 2032
Size: 3644
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 454 486 454
Size: 32 3612
AlwNI CAG_nnn'CTG
Cuts at: 420 597 949 2516 420
Size: 177 352 1567 1548
Fragments arranged by size:
1567 1548 352 177
ApaLI G'TGCA_C
Cuts at: 2414 2414
Size: 3644
ApoI r'AATT_y
Cuts at: 648 2997 3253 648
Size: 2349 256 1039
Fragments arranged by size:
2349 1039 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3644
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
AvaI C'yCGr_G
Cuts at: 664 664
Size: 3644
BamHI G'GATC_C
Cuts at: 669 669
Size: 3644
BanI G'GyrC_C
Cuts at: 660 1074 1322 1357 660
Size: 414 248 35 2947
Fragments arranged by size:
2947 414 248 35
BanII G_rGCy'C
Cuts at: 658 1688 658
Size: 1030 2614
BbeI G_GCGC'C
Cuts at: 1078 1326 1078
Size: 248 3396
BbvCI CC'TCA_GC
Cuts at: 421 421
Size: 3644
BciVI GTATCCnnnnn_n'
Cuts at: 1542 2303 3644 1542
Size: 761 1341 1542
Fragments arranged by size:
1542 1341 761
BclI T'GATC_A
Cuts at: 1164 1164
Size: 3644
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3644
BglII A'GATC_T
Cuts at: 327 1159 3196 327
Size: 832 2037 775
Fragments arranged by size:
2037 832 775
BlpI GC'TnA_GC
Cuts at: 756 756
Size: 3644
Bme1580I G_kGCm'C
Cuts at: 1269 1362 2418 1269
Size: 93 1056 2495
Fragments arranged by size:
2495 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 209 856 937 1272 3416 209
Size: 647 81 335 2144 437
Fragments arranged by size:
2144 647 437 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2011
Size: 3644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 472
Size: 3644
Bpu10I CC'TnA_GC
Cuts at: 421 421
Size: 3644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 1699 2191 2489 820
Size: 879 492 298 1975
Fragments arranged by size:
1975 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3348 3348
Size: 3644
BsaAI yAC'GTr
Cuts at: 997 997
Size: 3644
BsaBI GATnn'nnATC
Cuts at: 6 176 1158 3195 6
Size: 170 982 2037 455
Fragments arranged by size:
2037 982 455 170
BsaHI Gr'CG_yC
Cuts at: 1075 1323 2025 3521 1075
Size: 248 702 1496 1198
Fragments arranged by size:
1496 1198 702 248
BsaWI w'CCGG_w
Cuts at: 833 1354 2032 2306 2453 2864 833
Size: 521 678 274 147 411 1613
Fragments arranged by size:
1613 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 454 484 454
Size: 30 3614
BseYI C'CCAG_C
Cuts at: 524 1069 1827 2404 2900 524
Size: 545 758 577 496 1268
Fragments arranged by size:
1268 758 577 545 496
BsiEI CG_ry'CG
Cuts at: 104 1186 1232 2440 104
Size: 1082 46 1208 1308
Fragments arranged by size:
1308 1208 1082 46
BsiHKAI G_wGCw'C
Cuts at: 447 658 1436 1626 2418 447
Size: 211 778 190 792 1673
Fragments arranged by size:
1673 792 778 211 190
Bsp1286I G_dGCh'C
Cuts at: 447 658 1269 1362 1436 1626 1688 2418 447
Size: 211 611 93 74 190 62 730 1673
Fragments arranged by size:
1673 730 611 211 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2864 2864
Size: 3644
BspHI T'CATG_A
Cuts at: 2820 2820
Size: 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 519 694 908 1210 1591 519
Size: 175 214 302 381 2572
Fragments arranged by size:
2572 381 302 214 175
BsrBI CCG'CTC
Cuts at: 50 1936 1990 3474 3639 50
Size: 1886 54 1484 165 55
Fragments arranged by size:
1886 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1556 1556
Size: 3644
BsrFI r'CCGG_y
Cuts at: 1642 1823 2032 1642
Size: 181 209 3254
Fragments arranged by size:
3254 209 181
BssSI C'ACGA_G
Cuts at: 1915 2273 1915
Size: 358 3286
BstAPI GCAn_nnn'nTGC
Cuts at: 1021 1021
Size: 3644
BstBI TT'CG_AA
Cuts at: 2005 2005
Size: 3644
BstYI r'GATC_y
Cuts at: 1 327 669 1136 1159 1494 1740 2752 2838
Size: 326 342 467 23 335 246 1012 86
Cuts at: 2838 2850 3196 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 342 335 326 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 140 312 140
Size: 172 3472
BtgI C'CryG_G
Cuts at: 107 107
Size: 3644
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1594 1775 1938 1594
Size: 181 163 3300
Fragments arranged by size:
3300 181 163
BtsI GCAGTG_nn'
Cuts at: 445 887 445
Size: 442 3202
DraI TTT'AAA
Cuts at: 643 643
Size: 3644
DraIII CAC_nnn'GTG
Cuts at: 543 543
Size: 3644
DrdI GACnn_nn'nnGTC
Cuts at: 1350 2208 1350
Size: 858 2786
EaeI y'GGCC_r
Cuts at: 1229 1403 1794 1821 3166 1229
Size: 174 391 27 1345 1707
Fragments arranged by size:
1707 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1229 1229
Size: 3644
EarI CTCTTCn'nnn_
Cuts at: 403 1667 1877 403
Size: 1264 210 2170
Fragments arranged by size:
2170 1264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1852 2162 2308 3454 1852
Size: 310 146 1146 2042
Fragments arranged by size:
2042 1146 310 146
EcoICRI GAG'CTC
Cuts at: 656 656
Size: 3644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1469 1901 2648 2916 472
Size: 997 432 747 268 1200
Fragments arranged by size:
1200 997 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 783 783
Size: 3644
EcoRI G'AATT_C
Cuts at: 648 648
Size: 3644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1663 1663
Size: 3644
FspI TGC'GCA
Cuts at: 1425 1425
Size: 3644
HaeII r_GCGC'y
Cuts at: 118 1078 1326 2348 3327 118
Size: 960 248 1022 979 435
Fragments arranged by size:
1022 979 960 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 573 605 1486 1516 1518 1548 491
Size: 32 50 32 881 30 2 30 2587
Fragments arranged by size:
2587 881 50 32 32 30 30 2
HincII GTy'rAC
Cuts at: 683 683
Size: 3644
KasI G'GCGC_C
Cuts at: 1074 1322 1074
Size: 248 3396
KpnI G_GTAC'C
Cuts at: 664 664
Size: 3644
MluI A'CGCG_T
Cuts at: 3077 3077
Size: 3644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2315 2499 2315
Size: 184 3460
MscI TGG'CCA
Cuts at: 1405 1405
Size: 3644
MslI CAynn'nnrTG
Cuts at: 1633 1760 1633
Size: 127 3517
MspA1I CmG'CkG
Cuts at: 755 1069 1429 2442 2687 755
Size: 314 360 1013 245 1712
Fragments arranged by size:
1712 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1825 1825
Size: 3644
NarI GG'CG_CC
Cuts at: 1075 1323 1075
Size: 248 3396
NcoI C'CATG_G
Cuts at: 107 107
Size: 3644
NgoMIV G'CCGG_C
Cuts at: 1823 1823
Size: 3644
NspI r_CATG'y
Cuts at: 697 1197 2104 697
Size: 500 907 2237
Fragments arranged by size:
2237 907 500
PciI A'CATG_T
Cuts at: 2100 2100
Size: 3644
PflMI CCAn_nnn'nTGG
Cuts at: 531 531
Size: 3644
PmeI GTTT'AAAC
Cuts at: 643 643
Size: 3644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 454 486 2783 2815 454
Size: 32 2297 32 1283
Fragments arranged by size:
2297 1283 32 32
PstI C_TGCA'G
Cuts at: 602 691 1376 602
Size: 89 685 2870
Fragments arranged by size:
2870 685 89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3644
PvuII CAG'CTG
Cuts at: 1069 1429 1069
Size: 360 3284
RsrII CG'GwC_CG
Cuts at: 1839 1839
Size: 3644
SacI G_AGCT'C
Cuts at: 658 658
Size: 3644
SalI G'TCGA_C
Cuts at: 681 681
Size: 3644
SapI GCTCTTCn'nnn_
Cuts at: 1667 1877 1667
Size: 210 3434
SbfI CC_TGCA'GG
Cuts at: 691 691
Size: 3644
ScaI AGT'ACT
Cuts at: 196 3161 196
Size: 2965 679
SfcI C'TryA_G
Cuts at: 33 598 687 1372 2365 2556 33
Size: 565 89 685 993 191 1121
Fragments arranged by size:
1121 993 685 565 191 89
SfoI GGC'GCC
Cuts at: 1076 1324 1076
Size: 248 3396
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
SmaI CCC'GGG
Cuts at: 666 666
Size: 3644
SmlI C'TyrA_G
Cuts at: 799 1714 2206 2468 799
Size: 915 492 262 1975
Fragments arranged by size:
1975 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3644
SphI G_CATG'C
Cuts at: 697 1197 697
Size: 500 3144
StuI AGG'CCT
Cuts at: 235 299 235
Size: 64 3580
StyI C'CwwG_G
Cuts at: 107 778 1132 107
Size: 671 354 2619
Fragments arranged by size:
2619 671 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1227 1360 1819 2028 1227
Size: 133 459 209 2843
Fragments arranged by size:
2843 459 209 133
TatI w'GTAC_w
Cuts at: 194 3159 194
Size: 2965 679
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 261 293 3161 3193 261
Size: 32 2868 32 712
Fragments arranged by size:
2868 712 32 32
Tth111I GACn'n_nGTC
Cuts at: 585 1441 585
Size: 856 2788
XbaI T'CTAG_A
Cuts at: 675 675
Size: 3644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 159 1139 159
Size: 980 2664
XmaI C'CCGG_G
Cuts at: 664 664
Size: 3644
XmnI GAAnn'nnTTC
Cuts at: 250 250
Size: 3644
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI