Restriction map for
pF1KB8105
(Circular) (Six-base) MAPSORT of: pF1KB8105.seq Check: 402 from: 1 to: 3464
pF1KB8105 3464 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 502 502
Size: 3464
Acc65I G'GTAC_C
Cuts at: 480 480
Size: 3464
AclI AA'CG_TT
Cuts at: 3304 3304
Size: 3464
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1289 1721 2468 2736 1289
Size: 432 747 268 2017
Fragments arranged by size:
2017 747 432 268
AflIII A'CryG_T
Cuts at: 816 1920 2897 816
Size: 1104 977 1383
Fragments arranged by size:
1383 1104 977
AgeI A'CCGG_T
Cuts at: 1852 1852
Size: 3464
AlwNI CAG_nnn'CTG
Cuts at: 769 2336 769
Size: 1567 1897
ApaLI G'TGCA_C
Cuts at: 2234 2234
Size: 3464
ApoI r'AATT_y
Cuts at: 468 2817 3073 468
Size: 2349 256 859
Fragments arranged by size:
2349 859 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3464
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3464
AvaI C'yCGr_G
Cuts at: 484 484
Size: 3464
BamHI G'GATC_C
Cuts at: 489 489
Size: 3464
BanI G'GyrC_C
Cuts at: 228 480 894 1142 1177 228
Size: 252 414 248 35 2515
Fragments arranged by size:
2515 414 252 248 35
BanII G_rGCy'C
Cuts at: 478 1508 478
Size: 1030 2434
BbeI G_GCGC'C
Cuts at: 898 1146 898
Size: 248 3216
BbsI GAAGACnn'nnnn_
Cuts at: 416 416
Size: 3464
BciVI GTATCCnnnnn_n'
Cuts at: 1362 2123 3464 1362
Size: 761 1341 1362
Fragments arranged by size:
1362 1341 761
BclI T'GATC_A
Cuts at: 984 984
Size: 3464
BglII A'GATC_T
Cuts at: 979 3016 979
Size: 2037 1427
BlpI GC'TnA_GC
Cuts at: 576 576
Size: 3464
Bme1580I G_kGCm'C
Cuts at: 263 1089 1182 2238 263
Size: 826 93 1056 1489
Fragments arranged by size:
1489 1056 826 93
BmrI ACTGGGnnnn_n'
Cuts at: 676 757 1092 3236 676
Size: 81 335 2144 904
Fragments arranged by size:
2144 904 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1831 1831
Size: 3464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1519 2011 2309 640
Size: 879 492 298 1795
Fragments arranged by size:
1795 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 312 3168 312
Size: 2856 608
BsaAI yAC'GTr
Cuts at: 817 817
Size: 3464
BsaBI GATnn'nnATC
Cuts at: 6 978 3015 6
Size: 972 2037 455
Fragments arranged by size:
2037 972 455
BsaHI Gr'CG_yC
Cuts at: 329 895 1143 1845 3341 329
Size: 566 248 702 1496 452
Fragments arranged by size:
1496 702 566 452 248
BsaWI w'CCGG_w
Cuts at: 170 183 299 653 1174 1852 2126 2273 2684
Size: 13 116 354 521 678 274 147 411
Cuts at: 2684 170
Size: 950
Fragments arranged by size:
950 678 521 411 354 274 147 116
13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 345 375 345
Size: 30 3434
BseYI C'CCAG_C
Cuts at: 889 1647 2224 2720 889
Size: 758 577 496 1633
Fragments arranged by size:
1633 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1006 1052 2260 104
Size: 902 46 1208 1308
Fragments arranged by size:
1308 1208 902 46
BsiHKAI G_wGCw'C
Cuts at: 478 1256 1446 2238 478
Size: 778 190 792 1704
Fragments arranged by size:
1704 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 263 478 1089 1182 1256 1446 1508 2238 263
Size: 215 611 93 74 190 62 730 1489
Fragments arranged by size:
1489 730 611 215 190 93 74 62
BspEI T'CCGG_A
Cuts at: 170 183 299 2684 170
Size: 13 116 2385 950
Fragments arranged by size:
2385 950 116 13
BspHI T'CATG_A
Cuts at: 402 2640 402
Size: 2238 1226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 514 728 1030 1411 514
Size: 214 302 381 2567
Fragments arranged by size:
2567 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1756 1810 3294 3459 50
Size: 1706 54 1484 165 55
Fragments arranged by size:
1706 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1376 1376
Size: 3464
BsrFI r'CCGG_y
Cuts at: 1462 1643 1852 1462
Size: 181 209 3074
Fragments arranged by size:
3074 209 181
BssSI C'ACGA_G
Cuts at: 1735 2093 1735
Size: 358 3106
BstAPI GCAn_nnn'nTGC
Cuts at: 841 841
Size: 3464
BstBI TT'CG_AA
Cuts at: 1825 1825
Size: 3464
BstYI r'GATC_y
Cuts at: 1 167 489 956 979 1314 1560 2572 2658
Size: 166 322 467 23 335 246 1012 86
Cuts at: 2658 2670 3016 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 322 246 166
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 323 323
Size: 3464
BtgI C'CryG_G
Cuts at: 377 377
Size: 3464
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1414 1595 1758 1414
Size: 181 163 3120
Fragments arranged by size:
3120 181 163
BtsI GCAGTG_nn'
Cuts at: 707 707
Size: 3464
DraI TTT'AAA
Cuts at: 463 463
Size: 3464
DrdI GACnn_nn'nnGTC
Cuts at: 1170 2028 1170
Size: 858 2606
EaeI y'GGCC_r
Cuts at: 279 1049 1223 1614 1641 2986 279
Size: 770 174 391 27 1345 757
Fragments arranged by size:
1345 770 757 391 174 27
EagI C'GGCC_G
Cuts at: 1049 1049
Size: 3464
EarI CTCTTCn'nnn_
Cuts at: 298 1487 1697 298
Size: 1189 210 2065
Fragments arranged by size:
2065 1189 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1672 1982 2128 3274 1672
Size: 310 146 1146 1862
Fragments arranged by size:
1862 1146 310 146
EcoICRI GAG'CTC
Cuts at: 476 476
Size: 3464
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1289 1721 2468 2736 1289
Size: 432 747 268 2017
Fragments arranged by size:
2017 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 192 603 192
Size: 411 3053
EcoRI G'AATT_C
Cuts at: 468 468
Size: 3464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1483 1483
Size: 3464
FspI TGC'GCA
Cuts at: 1245 1245
Size: 3464
HaeII r_GCGC'y
Cuts at: 898 1146 2168 3147 898
Size: 248 1022 979 1215
Fragments arranged by size:
1215 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 152 184 1306 1336 1338 1368 152
Size: 32 1122 30 2 30 2248
Fragments arranged by size:
2248 1122 32 30 30 2
HincII GTy'rAC
Cuts at: 348 503 348
Size: 155 3309
KasI G'GCGC_C
Cuts at: 894 1142 894
Size: 248 3216
KpnI G_GTAC'C
Cuts at: 484 484
Size: 3464
MluI A'CGCG_T
Cuts at: 2897 2897
Size: 3464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2135 2319 2135
Size: 184 3280
MscI TGG'CCA
Cuts at: 281 1225 281
Size: 944 2520
MslI CAynn'nnrTG
Cuts at: 1453 1580 1453
Size: 127 3337
MspA1I CmG'CkG
Cuts at: 575 889 1249 2262 2507 575
Size: 314 360 1013 245 1532
Fragments arranged by size:
1532 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1645 1645
Size: 3464
NarI GG'CG_CC
Cuts at: 895 1143 895
Size: 248 3216
NcoI C'CATG_G
Cuts at: 377 377
Size: 3464
NgoMIV G'CCGG_C
Cuts at: 1643 1643
Size: 3464
NspI r_CATG'y
Cuts at: 517 1017 1924 517
Size: 500 907 2057
Fragments arranged by size:
2057 907 500
PciI A'CATG_T
Cuts at: 1920 1920
Size: 3464
PmeI GTTT'AAAC
Cuts at: 463 463
Size: 3464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2603 2635 2603
Size: 32 3432
PpuMI rG'GwC_Cy
Cuts at: 192 192
Size: 3464
PstI C_TGCA'G
Cuts at: 511 1196 511
Size: 685 2779
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3464
PvuII CAG'CTG
Cuts at: 889 1249 889
Size: 360 3104
RsrII CG'GwC_CG
Cuts at: 1659 1659
Size: 3464
SacI G_AGCT'C
Cuts at: 478 478
Size: 3464
SalI G'TCGA_C
Cuts at: 501 501
Size: 3464
SanDI GG'GwC_CC
Cuts at: 192 192
Size: 3464
SapI GCTCTTCn'nnn_
Cuts at: 1487 1697 1487
Size: 210 3254
SbfI CC_TGCA'GG
Cuts at: 511 511
Size: 3464
ScaI AGT'ACT
Cuts at: 2981 2981
Size: 3464
SfcI C'TryA_G
Cuts at: 33 432 507 1192 2185 2376 33
Size: 399 75 685 993 191 1121
Fragments arranged by size:
1121 993 685 399 191 75
SfoI GGC'GCC
Cuts at: 896 1144 896
Size: 248 3216
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3464
SmaI CCC'GGG
Cuts at: 486 486
Size: 3464
SmlI C'TyrA_G
Cuts at: 619 1534 2026 2288 619
Size: 915 492 262 1795
Fragments arranged by size:
1795 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3464
SphI G_CATG'C
Cuts at: 517 1017 517
Size: 500 2964
StyI C'CwwG_G
Cuts at: 377 598 952 377
Size: 221 354 2889
Fragments arranged by size:
2889 354 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1047 1180 1639 1848 1047
Size: 133 459 209 2663
Fragments arranged by size:
2663 459 209 133
TatI w'GTAC_w
Cuts at: 119 2979 119
Size: 2860 604
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 345 377 2981 3013 345
Size: 32 2604 32 796
Fragments arranged by size:
2604 796 32 32
Tth111I GACn'n_nGTC
Cuts at: 190 1261 190
Size: 1071 2393
XbaI T'CTAG_A
Cuts at: 495 495
Size: 3464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 375 959 375
Size: 584 2880
XmaI C'CCGG_G
Cuts at: 484 484
Size: 3464
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI