Restriction map for
pF1KB8104
(Circular) (Six-base) MAPSORT of: pF1KB8104.seq Check: 217 from: 1 to: 4496
pF1KB8104 4496 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 455 1158 455
Size: 703 3793
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1019 1260 2321 2753 3500 3768 751
Size: 268 241 1061 432 747 268 1479
Fragments arranged by size:
1479 1061 747 432 268 268 241
AfeI AGC'GCT
Cuts at: 661 736 754 661
Size: 75 18 4403
Fragments arranged by size:
4403 75 18
AflIII A'CryG_T
Cuts at: 968 1848 2952 3929 968
Size: 880 1104 977 1535
Fragments arranged by size:
1535 1104 977 880
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 858 1392 1801 3368 858
Size: 534 409 1567 1986
Fragments arranged by size:
1986 1567 534 409
ApaI G_GGCC'C
Cuts at: 127 186 276 127
Size: 59 90 4347
Fragments arranged by size:
4347 90 59
ApaLI G'TGCA_C
Cuts at: 1315 3266 1315
Size: 1951 2545
ApoI r'AATT_y
Cuts at: 1500 3849 4105 1500
Size: 2349 256 1891
Fragments arranged by size:
2349 1891 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 118 168 269 508 1516 118
Size: 50 101 239 1008 3098
Fragments arranged by size:
3098 1008 239 101 50
BamHI G'GATC_C
Cuts at: 993 1521 993
Size: 528 3968
BanI G'GyrC_C
Cuts at: 712 1512 1926 2174 2209 712
Size: 800 414 248 35 2999
Fragments arranged by size:
2999 800 414 248 35
BanII G_rGCy'C
Cuts at: 127 186 269 276 327 374 439 1510 2540
Size: 59 83 7 51 47 65 1071 1030
Cuts at: 2540 127
Size: 2083
Fragments arranged by size:
2083 1071 1030 83 65 59 51 47
7
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbvCI CC'TCA_GC
Cuts at: 347 1216 347
Size: 869 3627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1071 1105 1156 1190 1071
Size: 34 51 34 4377
Fragments arranged by size:
4377 51 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1275 2394 3155 4496 1275
Size: 1119 761 1341 1275
Fragments arranged by size:
1341 1275 1119 761
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BglI GCCn_nnn'nGGC
Cuts at: 131 280 1480 131
Size: 149 1200 3147
Fragments arranged by size:
3147 1200 149
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 532 1608 532
Size: 1076 3420
Bme1580I G_kGCm'C
Cuts at: 127 186 276 383 689 715 1319 2121 2214
Size: 59 90 107 306 26 604 802 93
Cuts at: 2214 3270 127
Size: 1056 1353
Fragments arranged by size:
1353 1056 802 604 306 107 93 90
59 26
BmgBI CAC'GTC
Cuts at: 378 588 378
Size: 210 4286
BmrI ACTGGGnnnn_n'
Cuts at: 701 1708 1789 2124 4268 701
Size: 1007 81 335 2144 929
Fragments arranged by size:
2144 1007 929 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1062 1279 196
Size: 866 217 3413
Fragments arranged by size:
3413 866 217
Bpu10I CC'TnA_GC
Cuts at: 347 1216 347
Size: 869 3627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2551 3043 3341 1672
Size: 879 492 298 2827
Fragments arranged by size:
2827 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 486 4200 486
Size: 3714 782
BsaAI yAC'GTr
Cuts at: 1849 1849
Size: 4496
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 905 1927 2175 2877 4373 905
Size: 1022 248 702 1496 1028
Fragments arranged by size:
1496 1028 1022 702 248
BsaWI w'CCGG_w
Cuts at: 617 1685 2206 2884 3158 3305 3716 617
Size: 1068 521 678 274 147 411 1397
Fragments arranged by size:
1397 1068 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 474 486 937 940 1027 1030 1297 1429
Size: 92 12 451 3 87 3 267 132
Cuts at: 1429 382
Size: 3449
Fragments arranged by size:
3449 451 267 132 92 87 12 3
3
BseYI C'CCAG_C
Cuts at: 1443 1921 2679 3256 3752 1443
Size: 478 758 577 496 2187
Fragments arranged by size:
2187 758 577 496 478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 694 586
Size: 108 4388
BsiEI CG_ry'CG
Cuts at: 104 145 242 251 2038 2084 3292 104
Size: 41 97 9 1787 46 1208 1308
Fragments arranged by size:
1787 1308 1208 97 46 41 9
BsiHKAI G_wGCw'C
Cuts at: 890 1022 1319 1510 2288 2478 3270 890
Size: 132 297 191 778 190 792 2116
Fragments arranged by size:
2116 792 778 297 191 190 132
Bsp1286I G_dGCh'C
Cuts at: 127 186 269 276 327 374 383 439 689
Size: 59 83 7 51 47 9 56 250
Cuts at: 689 715 890 1022 1319 1510 2121 2214 2288
Size: 26 175 132 297 191 611 93 74
Cuts at: 2288 2478 2540 3270 127
Size: 190 62 730 1353
Fragments arranged by size:
1353 730 611 297 250 191 190 175
132 93 83 74 62 59 56 51
47 26 9 7
BspEI T'CCGG_A
Cuts at: 617 3716 617
Size: 3099 1397
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 455 536 851 866 1158 1546 1760 2062 2443
Size: 81 315 15 292 388 214 302 381
Cuts at: 2443 455
Size: 2508
Fragments arranged by size:
2508 388 381 315 302 292 214 81
15
BsrBI CCG'CTC
Cuts at: 50 180 408 948 2788 2842 4326 4491 50
Size: 130 228 540 1840 54 1484 165 55
Fragments arranged by size:
1840 1484 540 228 165 130 55 54
BsrDI GCAATG_nn'
Cuts at: 2408 2408
Size: 4496
BsrFI r'CCGG_y
Cuts at: 636 1336 1400 2494 2675 2884 636
Size: 700 64 1094 181 209 2248
Fragments arranged by size:
2248 1094 700 209 181 64
BsrGI T'GTAC_A
Cuts at: 971 971
Size: 4496
BssHII G'CGCG_C
Cuts at: 226 1029 226
Size: 803 3693
BssSI C'ACGA_G
Cuts at: 1285 2767 3125 1285
Size: 1482 358 2656
Fragments arranged by size:
2656 1482 358
BstAPI GCAn_nnn'nTGC
Cuts at: 858 1873 858
Size: 1015 3481
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstYI r'GATC_y
Cuts at: 1 614 993 1244 1388 1521 1988 2011 2346
Size: 613 379 251 144 133 467 23 335
Cuts at: 2346 2592 3604 3690 3702 4048 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 613 467 449 379 346 335 251
246 144 133 86 23 12
BtgI C'CryG_G
Cuts at: 257 276 319 555 623 810 1007 257
Size: 19 43 236 68 187 197 3746
Fragments arranged by size:
3746 236 197 187 68 43 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1315 2446 2627 2790 1315
Size: 1131 181 163 3021
Fragments arranged by size:
3021 1131 181 163
BtsI GCAGTG_nn'
Cuts at: 1063 1323 1739 1063
Size: 260 416 3820
Fragments arranged by size:
3820 416 260
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 273 308 273
Size: 35 4461
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DraIII CAC_nnn'GTG
Cuts at: 816 1290 816
Size: 474 4022
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 142 248 1383 1472 2081 2255 2646 2673 4018
Size: 106 1135 89 609 174 391 27 1345
Cuts at: 4018 142
Size: 620
Fragments arranged by size:
1345 1135 620 609 391 174 106 89
27
EagI C'GGCC_G
Cuts at: 142 248 2081 142
Size: 106 1833 2557
Fragments arranged by size:
2557 1833 106
EarI CTCTTCn'nnn_
Cuts at: 223 444 1181 2519 2729 223
Size: 221 737 1338 210 1990
Fragments arranged by size:
1990 1338 737 221 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1508 1508
Size: 4496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 751 1019 1062 1260 1279 2321 2753 3500
Size: 555 268 43 198 19 1042 432 747
Cuts at: 3500 3768 196
Size: 268 924
Fragments arranged by size:
1042 924 747 555 432 268 268 198
43 19
EcoNI CCTnn'n_nnAGG
Cuts at: 922 922
Size: 4496
EcoO109I rG'GnC_Cy
Cuts at: 123 158 183 303 855 1635 123
Size: 35 25 120 552 780 2984
Fragments arranged by size:
2984 780 552 120 35 25
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4496
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 663 738 756 1930 2178 3200 4179 663
Size: 75 18 1174 248 1022 979 980
Fragments arranged by size:
1174 1022 980 979 248 75 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1209 1241 2338 2368 2370 2400 1209
Size: 32 1097 30 2 30 3305
Fragments arranged by size:
3305 1097 32 30 30 2
HincII GTy'rAC
Cuts at: 1535 1535
Size: 4496
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 1385 1474 2257 1385
Size: 89 783 3624
Fragments arranged by size:
3624 783 89
MslI CAynn'nnrTG
Cuts at: 2485 2612 2485
Size: 127 4369
MspA1I CmG'CkG
Cuts at: 259 278 321 351 433 557 570 678 719
Size: 19 43 30 82 124 13 108 41
Cuts at: 719 849 1607 1921 2281 3294 3539 259
Size: 130 758 314 360 1013 245 1216
Fragments arranged by size:
1216 1013 758 360 314 245 130 124
108 82 43 41 30 19 13
NaeI GCC'GGC
Cuts at: 1338 2677 1338
Size: 1339 3157
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 623 623
Size: 4496
NgoMIV G'CCGG_C
Cuts at: 1336 2675 1336
Size: 1339 3157
NotI GC'GGCC_GC
Cuts at: 248 248
Size: 4496
NspI r_CATG'y
Cuts at: 972 1056 1549 2049 2956 972
Size: 84 493 500 907 2512
Fragments arranged by size:
2512 907 500 493 84
PasI CC'CwG_GG
Cuts at: 394 1409 394
Size: 1015 3481
PciI A'CATG_T
Cuts at: 968 2952 968
Size: 1984 2512
PflMI CCAn_nnn'nTGG
Cuts at: 816 1392 816
Size: 576 3920
PfoI T'CCnGG_A
Cuts at: 1247 1391 1247
Size: 144 4352
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PpuMI rG'GwC_Cy
Cuts at: 303 855 303
Size: 552 3944
PshAI GACnn'nnGTC
Cuts at: 590 625 590
Size: 35 4461
PspOMI G'GGCC_C
Cuts at: 123 182 272 123
Size: 59 90 4347
Fragments arranged by size:
4347 90 59
PspXI vC'TCGA_Gb
Cuts at: 508 508
Size: 4496
PstI C_TGCA'G
Cuts at: 166 452 848 854 1135 1241 1543 2228 166
Size: 286 396 6 281 106 302 685 2434
Fragments arranged by size:
2434 685 396 302 286 281 106 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 351 570 678 849 1921 2281 351
Size: 219 108 171 1072 360 2566
Fragments arranged by size:
2566 1072 360 219 171 108
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1510 1510
Size: 4496
SacII CC_GC'GG
Cuts at: 260 279 322 558 260
Size: 19 43 236 4198
Fragments arranged by size:
4198 236 43 19
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 223 444 2519 2729 223
Size: 221 2075 210 1990
Fragments arranged by size:
2075 1990 221 210
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 162 448 844 850 1131 1237 1539 2224
Size: 129 286 396 6 281 106 302 685
Cuts at: 2224 3217 3408 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 396 302 286 281 191
129 106 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 280 280
Size: 4496
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SgrAI Cr'CCGG_yG
Cuts at: 1336 1400 1336
Size: 64 4432
SmaI CCC'GGG
Cuts at: 120 170 271 1518 120
Size: 50 101 1247 3098
Fragments arranged by size:
3098 1247 101 50
SmlI C'TyrA_G
Cuts at: 508 1651 2566 3058 3320 508
Size: 1143 915 492 262 1684
Fragments arranged by size:
1684 1143 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
StyI C'CwwG_G
Cuts at: 623 935 1630 1984 623
Size: 312 695 354 3135
Fragments arranged by size:
3135 695 354 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 468 660 2079 2212 2671 2880 468
Size: 192 1419 133 459 209 2084
Fragments arranged by size:
2084 1419 459 209 192 133
TatI w'GTAC_w
Cuts at: 971 4011 971
Size: 3040 1456
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 869 901 4013 4045 869
Size: 32 3112 32 1320
Fragments arranged by size:
3112 1320 32 32
Tth111I GACn'n_nGTC
Cuts at: 2293 2293
Size: 4496
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1991 1991
Size: 4496
XhoI C'TCGA_G
Cuts at: 508 508
Size: 4496
XmaI C'CCGG_G
Cuts at: 118 168 269 1516 118
Size: 50 101 1247 3098
Fragments arranged by size:
3098 1247 101 50
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BmtI BsaXI BsiWI BsmI BsmBI
BstEII BstXI BstZ17I Bsu36I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NruI NsiI PacI
PmlI PsiI PsrI SanDI SexAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI