Restriction map for
pF1KB8103
(Circular) (Six-base) MAPSORT of: pF1KB8103.seq Check: 1623 from: 1 to: 3347
pF1KB8103 3347 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 385 385
Size: 3347
Acc65I G'GTAC_C
Cuts at: 363 363
Size: 3347
AclI AA'CG_TT
Cuts at: 3187 3187
Size: 3347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1172 1604 2351 2619 1172
Size: 432 747 268 1900
Fragments arranged by size:
1900 747 432 268
AflIII A'CryG_T
Cuts at: 699 1803 2780 699
Size: 1104 977 1266
Fragments arranged by size:
1266 1104 977
AgeI A'CCGG_T
Cuts at: 1735 1735
Size: 3347
AlwNI CAG_nnn'CTG
Cuts at: 652 2219 652
Size: 1567 1780
ApaLI G'TGCA_C
Cuts at: 2117 2117
Size: 3347
ApoI r'AATT_y
Cuts at: 351 2700 2956 351
Size: 2349 256 742
Fragments arranged by size:
2349 742 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3347
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3347
AvaI C'yCGr_G
Cuts at: 367 367
Size: 3347
BamHI G'GATC_C
Cuts at: 372 372
Size: 3347
BanI G'GyrC_C
Cuts at: 217 327 363 777 1025 1060 217
Size: 110 36 414 248 35 2504
Fragments arranged by size:
2504 414 248 110 36 35
BanII G_rGCy'C
Cuts at: 148 361 1391 148
Size: 213 1030 2104
Fragments arranged by size:
2104 1030 213
BbeI G_GCGC'C
Cuts at: 221 781 1029 221
Size: 560 248 2539
Fragments arranged by size:
2539 560 248
BciVI GTATCCnnnnn_n'
Cuts at: 1245 2006 3347 1245
Size: 761 1341 1245
Fragments arranged by size:
1341 1245 761
BclI T'GATC_A
Cuts at: 867 867
Size: 3347
BglI GCCn_nnn'nGGC
Cuts at: 152 257 152
Size: 105 3242
BglII A'GATC_T
Cuts at: 176 862 2899 176
Size: 686 2037 624
Fragments arranged by size:
2037 686 624
BlpI GC'TnA_GC
Cuts at: 142 459 142
Size: 317 3030
Bme1580I G_kGCm'C
Cuts at: 972 1065 2121 972
Size: 93 1056 2198
Fragments arranged by size:
2198 1056 93
BmgBI CAC'GTC
Cuts at: 168 303 168
Size: 135 3212
BmrI ACTGGGnnnn_n'
Cuts at: 559 640 975 3119 559
Size: 81 335 2144 787
Fragments arranged by size:
2144 787 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1714 1714
Size: 3347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 1402 1894 2192 523
Size: 879 492 298 1678
Fragments arranged by size:
1678 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3051 3051
Size: 3347
BsaAI yAC'GTr
Cuts at: 700 700
Size: 3347
BsaBI GATnn'nnATC
Cuts at: 6 861 2898 6
Size: 855 2037 455
Fragments arranged by size:
2037 855 455
BsaHI Gr'CG_yC
Cuts at: 218 778 1026 1728 3224 218
Size: 560 248 702 1496 341
Fragments arranged by size:
1496 702 560 341 248
BsaWI w'CCGG_w
Cuts at: 536 1057 1735 2009 2156 2567 536
Size: 521 678 274 147 411 1316
Fragments arranged by size:
1316 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 246 772 1530 2107 2603 246
Size: 526 758 577 496 990
Fragments arranged by size:
990 758 577 526 496
BsiEI CG_ry'CG
Cuts at: 104 238 261 889 935 2143 104
Size: 134 23 628 46 1208 1308
Fragments arranged by size:
1308 1208 628 134 46 23
BsiHKAI G_wGCw'C
Cuts at: 361 1139 1329 2121 361
Size: 778 190 792 1587
Fragments arranged by size:
1587 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 148 361 972 1065 1139 1329 1391 2121 148
Size: 213 611 93 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 213 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2567 2567
Size: 3347
BspHI T'CATG_A
Cuts at: 2523 2523
Size: 3347
BspMI ACCTGCnnnn'nnnn_
Cuts at: 397 611 913 1294 397
Size: 214 302 381 2450
Fragments arranged by size:
2450 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1639 1693 3177 3342 50
Size: 1589 54 1484 165 55
Fragments arranged by size:
1589 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1259 1259
Size: 3347
BsrFI r'CCGG_y
Cuts at: 1345 1526 1735 1345
Size: 181 209 2957
Fragments arranged by size:
2957 209 181
BssSI C'ACGA_G
Cuts at: 1618 1976 1618
Size: 358 2989
BstAPI GCAn_nnn'nTGC
Cuts at: 724 724
Size: 3347
BstBI TT'CG_AA
Cuts at: 1708 1708
Size: 3347
BstYI r'GATC_y
Cuts at: 1 176 372 839 862 1197 1443 2455 2541
Size: 175 196 467 23 335 246 1012 86
Cuts at: 2541 2553 2899 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 196 175
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1297 1478 1641 1297
Size: 181 163 3003
Fragments arranged by size:
3003 181 163
BtsI GCAGTG_nn'
Cuts at: 590 590
Size: 3347
DraI TTT'AAA
Cuts at: 346 346
Size: 3347
DrdI GACnn_nn'nnGTC
Cuts at: 1053 1911 1053
Size: 858 2489
EaeI y'GGCC_r
Cuts at: 235 258 932 1106 1497 1524 2869 235
Size: 23 674 174 391 27 1345 713
Fragments arranged by size:
1345 713 674 391 174 27 23
EagI C'GGCC_G
Cuts at: 235 258 932 235
Size: 23 674 2650
Fragments arranged by size:
2650 674 23
EarI CTCTTCn'nnn_
Cuts at: 1370 1580 1370
Size: 210 3137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1555 1865 2011 3157 1555
Size: 310 146 1146 1745
Fragments arranged by size:
1745 1146 310 146
EcoICRI GAG'CTC
Cuts at: 359 359
Size: 3347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1172 1604 2351 2619 1172
Size: 432 747 268 1900
Fragments arranged by size:
1900 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 486 486
Size: 3347
EcoRI G'AATT_C
Cuts at: 351 351
Size: 3347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1366 1366
Size: 3347
FspI TGC'GCA
Cuts at: 1128 1128
Size: 3347
HaeII r_GCGC'y
Cuts at: 221 781 1029 2051 3030 221
Size: 560 248 1022 979 538
Fragments arranged by size:
1022 979 560 538 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1189 1219 1221 1251 1189
Size: 30 2 30 3285
Fragments arranged by size:
3285 30 30 2
HincII GTy'rAC
Cuts at: 386 386
Size: 3347
KasI G'GCGC_C
Cuts at: 217 777 1025 217
Size: 560 248 2539
Fragments arranged by size:
2539 560 248
KpnI G_GTAC'C
Cuts at: 367 367
Size: 3347
MluI A'CGCG_T
Cuts at: 2780 2780
Size: 3347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 2018 2202 92
Size: 1926 184 1237
Fragments arranged by size:
1926 1237 184
MscI TGG'CCA
Cuts at: 1108 1108
Size: 3347
MslI CAynn'nnrTG
Cuts at: 1336 1463 1336
Size: 127 3220
MspA1I CmG'CkG
Cuts at: 458 772 1132 2145 2390 458
Size: 314 360 1013 245 1415
Fragments arranged by size:
1415 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1528 1528
Size: 3347
NarI GG'CG_CC
Cuts at: 218 778 1026 218
Size: 560 248 2539
Fragments arranged by size:
2539 560 248
NgoMIV G'CCGG_C
Cuts at: 1526 1526
Size: 3347
NotI GC'GGCC_GC
Cuts at: 235 235
Size: 3347
NspI r_CATG'y
Cuts at: 400 900 1807 400
Size: 500 907 1940
Fragments arranged by size:
1940 907 500
PciI A'CATG_T
Cuts at: 1803 1803
Size: 3347
PmeI GTTT'AAAC
Cuts at: 346 346
Size: 3347
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2486 2518 2486
Size: 32 3315
PstI C_TGCA'G
Cuts at: 197 394 1079 197
Size: 197 685 2465
Fragments arranged by size:
2465 685 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3347
PvuII CAG'CTG
Cuts at: 772 1132 772
Size: 360 2987
RsrII CG'GwC_CG
Cuts at: 1542 1542
Size: 3347
SacI G_AGCT'C
Cuts at: 361 361
Size: 3347
SalI G'TCGA_C
Cuts at: 384 384
Size: 3347
SapI GCTCTTCn'nnn_
Cuts at: 1370 1580 1370
Size: 210 3137
SbfI CC_TGCA'GG
Cuts at: 197 394 197
Size: 197 3150
ScaI AGT'ACT
Cuts at: 2864 2864
Size: 3347
SfcI C'TryA_G
Cuts at: 33 193 390 1075 2068 2259 33
Size: 160 197 685 993 191 1121
Fragments arranged by size:
1121 993 685 197 191 160
SfiI GGCCn_nnn'nGGCC
Cuts at: 257 257
Size: 3347
SfoI GGC'GCC
Cuts at: 219 779 1027 219
Size: 560 248 2539
Fragments arranged by size:
2539 560 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3347
SmaI CCC'GGG
Cuts at: 369 369
Size: 3347
SmlI C'TyrA_G
Cuts at: 502 1417 1909 2171 502
Size: 915 492 262 1678
Fragments arranged by size:
1678 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3347
SphI G_CATG'C
Cuts at: 400 900 400
Size: 500 2847
StyI C'CwwG_G
Cuts at: 481 835 481
Size: 354 2993
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 930 1063 1522 1731 334
Size: 596 133 459 209 1950
Fragments arranged by size:
1950 596 459 209 133
TatI w'GTAC_w
Cuts at: 2862 2862
Size: 3347
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 3315
Tth111I GACn'n_nGTC
Cuts at: 1144 1144
Size: 3347
XbaI T'CTAG_A
Cuts at: 378 378
Size: 3347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 842 842
Size: 3347
XmaI C'CCGG_G
Cuts at: 367 367
Size: 3347
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NotI NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI