Restriction map for
pF1KB8102
(Circular) (Six-base) MAPSORT of: pF1KB8102.seq Check: 2905 from: 1 to: 4364
pF1KB8102 4364 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1402 1402
Size: 4364
Acc65I G'GTAC_C
Cuts at: 1380 1380
Size: 4364
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 2189 2621 3368 3636 642
Size: 1547 432 747 268 1370
Fragments arranged by size:
1547 1370 747 432 268
AfeI AGC'GCT
Cuts at: 1088 1088
Size: 4364
AflIII A'CryG_T
Cuts at: 433 1716 2820 3797 433
Size: 1283 1104 977 1000
Fragments arranged by size:
1283 1104 1000 977
AgeI A'CCGG_T
Cuts at: 2752 2752
Size: 4364
AleI CACnn'nnGTG
Cuts at: 325 859 1139 325
Size: 534 280 3550
Fragments arranged by size:
3550 534 280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 835 835
Size: 4364
AlwNI CAG_nnn'CTG
Cuts at: 344 1669 3236 344
Size: 1325 1567 1472
Fragments arranged by size:
1567 1472 1325
ApaLI G'TGCA_C
Cuts at: 3134 3134
Size: 4364
ApoI r'AATT_y
Cuts at: 1368 3717 3973 1368
Size: 2349 256 1759
Fragments arranged by size:
2349 1759 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4364
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 1384 1384
Size: 4364
BamHI G'GATC_C
Cuts at: 1389 1389
Size: 4364
BanI G'GyrC_C
Cuts at: 580 784 916 952 1276 1380 1794 2042 2077
Size: 204 132 36 324 104 414 248 35
Cuts at: 2077 580
Size: 2867
Fragments arranged by size:
2867 414 324 248 204 132 104 36
35
BanII G_rGCy'C
Cuts at: 505 757 1378 2408 505
Size: 252 621 1030 2461
Fragments arranged by size:
2461 1030 621 252
BbeI G_GCGC'C
Cuts at: 920 1798 2046 920
Size: 878 248 3238
Fragments arranged by size:
3238 878 248
BciVI GTATCCnnnnn_n'
Cuts at: 2262 3023 4364 2262
Size: 761 1341 2262
Fragments arranged by size:
2262 1341 761
BclI T'GATC_A
Cuts at: 1884 1884
Size: 4364
BfrBI ATG'CAT
Cuts at: 177 177
Size: 4364
BglI GCCn_nnn'nGGC
Cuts at: 112 1214 112
Size: 1102 3262
BglII A'GATC_T
Cuts at: 1879 3916 1879
Size: 2037 2327
BlpI GC'TnA_GC
Cuts at: 1476 1476
Size: 4364
Bme1580I G_kGCm'C
Cuts at: 749 955 1281 1989 2082 3138 749
Size: 206 326 708 93 1056 1975
Fragments arranged by size:
1975 1056 708 326 206 93
BmgBI CAC'GTC
Cuts at: 1137 1137
Size: 4364
BmrI ACTGGGnnnn_n'
Cuts at: 365 1576 1657 1992 4136 365
Size: 1211 81 335 2144 593
Fragments arranged by size:
2144 1211 593 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 4364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 955
Size: 4364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 2419 2911 3209 1540
Size: 879 492 298 2695
Fragments arranged by size:
2695 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1043 4068 1043
Size: 3025 1339
BsaAI yAC'GTr
Cuts at: 1717 1717
Size: 4364
BsaBI GATnn'nnATC
Cuts at: 6 1261 1878 3915 6
Size: 1255 617 2037 455
Fragments arranged by size:
2037 1255 617 455
BsaHI Gr'CG_yC
Cuts at: 917 1043 1795 2043 2745 4241 917
Size: 126 752 248 702 1496 1040
Fragments arranged by size:
1496 1040 752 702 248 126
BsaWI w'CCGG_w
Cuts at: 1553 2074 2752 3026 3173 3584 1553
Size: 521 678 274 147 411 2333
Fragments arranged by size:
2333 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 325 355 325
Size: 30 4334
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1144 1240 1347 1144
Size: 96 107 4161
Fragments arranged by size:
4161 107 96
BseYI C'CCAG_C
Cuts at: 724 1789 2547 3124 3620 724
Size: 1065 758 577 496 1468
Fragments arranged by size:
1468 1065 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 925
Size: 4364
BsiEI CG_ry'CG
Cuts at: 104 1906 1952 3160 104
Size: 1802 46 1208 1308
Fragments arranged by size:
1802 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 332 505 1136 1378 2156 2346 3138 332
Size: 173 631 242 778 190 792 1558
Fragments arranged by size:
1558 792 778 631 242 190 173
BsmI GAATG_Cn'
Cuts at: 990 990
Size: 4364
Bsp1286I G_dGCh'C
Cuts at: 332 505 749 757 955 1136 1281 1378 1989
Size: 173 244 8 198 181 145 97 611
Cuts at: 1989 2082 2156 2346 2408 3138 332
Size: 93 74 190 62 730 1558
Fragments arranged by size:
1558 730 611 244 198 190 181 173
145 97 93 74 62 8
BspEI T'CCGG_A
Cuts at: 3584 3584
Size: 4364
BspHI T'CATG_A
Cuts at: 452 3540 452
Size: 3088 1276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 314 805 1414 1628 1930 2311 314
Size: 491 609 214 302 381 2367
Fragments arranged by size:
2367 609 491 381 302 214
BsrBI CCG'CTC
Cuts at: 50 782 2656 2710 4194 4359 50
Size: 732 1874 54 1484 165 55
Fragments arranged by size:
1874 1484 732 165 55 54
BsrDI GCAATG_nn'
Cuts at: 200 2276 200
Size: 2076 2288
BsrFI r'CCGG_y
Cuts at: 998 2362 2543 2752 998
Size: 1364 181 209 2610
Fragments arranged by size:
2610 1364 209 181
BssSI C'ACGA_G
Cuts at: 376 420 2635 2993 376
Size: 44 2215 358 1747
Fragments arranged by size:
2215 1747 358 44
BstAPI GCAn_nnn'nTGC
Cuts at: 1741 1741
Size: 4364
BstBI TT'CG_AA
Cuts at: 2725 2725
Size: 4364
BstXI CCAn_nnnn'nTGG
Cuts at: 1269 1269
Size: 4364
BstYI r'GATC_y
Cuts at: 1 1389 1856 1879 2214 2460 3472 3558 3570
Size: 1388 467 23 335 246 1012 86 12
Cuts at: 3570 3916 1
Size: 346 449
Fragments arranged by size:
1388 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4364
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1248 1293 2314 2495 2658 1248
Size: 45 1021 181 163 2954
Fragments arranged by size:
2954 1021 181 163 45
BtsI GCAGTG_nn'
Cuts at: 1607 1607
Size: 4364
DraI TTT'AAA
Cuts at: 1062 1363 1062
Size: 301 4063
DraIII CAC_nnn'GTG
Cuts at: 679 927 679
Size: 248 4116
DrdI GACnn_nn'nnGTC
Cuts at: 2070 2928 2070
Size: 858 3506
EaeI y'GGCC_r
Cuts at: 236 806 899 1206 1949 2123 2514 2541 3886
Size: 570 93 307 743 174 391 27 1345
Cuts at: 3886 236
Size: 714
Fragments arranged by size:
1345 743 714 570 391 307 174 93
27
EagI C'GGCC_G
Cuts at: 1949 1949
Size: 4364
EarI CTCTTCn'nnn_
Cuts at: 387 973 1324 2387 2597 387
Size: 586 351 1063 210 2154
Fragments arranged by size:
2154 1063 586 351 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2572 2882 3028 4174 2572
Size: 310 146 1146 2762
Fragments arranged by size:
2762 1146 310 146
EcoICRI GAG'CTC
Cuts at: 503 1376 503
Size: 873 3491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 955 2189 2621 3368 3636 642
Size: 313 1234 432 747 268 1370
Fragments arranged by size:
1370 1234 747 432 313 268
EcoNI CCTnn'n_nnAGG
Cuts at: 840 840
Size: 4364
EcoO109I rG'GnC_Cy
Cuts at: 794 1503 794
Size: 709 3655
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4364
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4364
HaeII r_GCGC'y
Cuts at: 128 712 920 1090 1798 2046 3068 4047 128
Size: 584 208 170 708 248 1022 979 445
Fragments arranged by size:
1022 979 708 584 445 248 208 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 389 421 2206 2236 2238 2268 295
Size: 32 62 32 1785 30 2 30 2391
Fragments arranged by size:
2391 1785 62 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1403 1403
Size: 4364
KasI G'GCGC_C
Cuts at: 916 1794 2042 916
Size: 878 248 3238
Fragments arranged by size:
3238 878 248
KpnI G_GTAC'C
Cuts at: 1384 1384
Size: 4364
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 671 3035 3219 671
Size: 2364 184 1816
Fragments arranged by size:
2364 1816 184
MscI TGG'CCA
Cuts at: 238 808 1208 2125 238
Size: 570 400 917 2477
Fragments arranged by size:
2477 917 570 400
MslI CAynn'nnrTG
Cuts at: 325 859 1139 1267 2353 2480 325
Size: 534 280 128 1086 127 2209
Fragments arranged by size:
2209 1086 534 280 128 127
MspA1I CmG'CkG
Cuts at: 825 905 1475 1789 2149 3162 3407 825
Size: 80 570 314 360 1013 245 1782
Fragments arranged by size:
1782 1013 570 360 314 245 80
NaeI GCC'GGC
Cuts at: 2545 2545
Size: 4364
NarI GG'CG_CC
Cuts at: 917 1795 2043 917
Size: 878 248 3238
Fragments arranged by size:
3238 878 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4364
NgoMIV G'CCGG_C
Cuts at: 2543 2543
Size: 4364
NsiI A_TGCA'T
Cuts at: 179 179
Size: 4364
NspI r_CATG'y
Cuts at: 1254 1417 1917 2824 1254
Size: 163 500 907 2794
Fragments arranged by size:
2794 907 500 163
PciI A'CATG_T
Cuts at: 2820 2820
Size: 4364
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 4332
PpuMI rG'GwC_Cy
Cuts at: 794 794
Size: 4364
PshAI GACnn'nnGTC
Cuts at: 371 371
Size: 4364
PsiI TTA'TAA
Cuts at: 467 467
Size: 4364
PstI C_TGCA'G
Cuts at: 824 1094 1411 2096 824
Size: 270 317 685 3092
Fragments arranged by size:
3092 685 317 270
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 825 1789 2149 825
Size: 964 360 3040
Fragments arranged by size:
3040 964 360
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 505 1378 505
Size: 873 3491
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4364
SapI GCTCTTCn'nnn_
Cuts at: 973 2387 2597 973
Size: 1414 210 2740
Fragments arranged by size:
2740 1414 210
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4364
ScaI AGT'ACT
Cuts at: 3881 3881
Size: 4364
SexAI A'CCwGG_T
Cuts at: 923 923
Size: 4364
SfcI C'TryA_G
Cuts at: 33 820 1090 1407 2092 3085 3276 33
Size: 787 270 317 685 993 191 1121
Fragments arranged by size:
1121 993 787 685 317 270 191
SfoI GGC'GCC
Cuts at: 918 1796 2044 918
Size: 878 248 3238
Fragments arranged by size:
3238 878 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SmaI CCC'GGG
Cuts at: 1386 1386
Size: 4364
SmlI C'TyrA_G
Cuts at: 1519 2434 2926 3188 1519
Size: 915 492 262 2695
Fragments arranged by size:
2695 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1254 1417 1917 1254
Size: 163 500 3701
Fragments arranged by size:
3701 500 163
StuI AGG'CCT
Cuts at: 693 693
Size: 4364
StyI C'CwwG_G
Cuts at: 107 1498 1852 107
Size: 1391 354 2619
Fragments arranged by size:
2619 1391 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 598 738 1261 1947 2080 2539 2748 598
Size: 140 523 686 133 459 209 2214
Fragments arranged by size:
2214 686 523 459 209 140 133
TatI w'GTAC_w
Cuts at: 770 3879 770
Size: 3109 1255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 473 505 3881 3913 473
Size: 32 3376 32 924
Fragments arranged by size:
3376 924 32 32
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4364
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1859 1859
Size: 4364
XmaI C'CCGG_G
Cuts at: 1384 1384
Size: 4364
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BmtI Bpu10I BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI