Restriction map for
pF1KB8101
(Circular) (Six-base) MAPSORT of: pF1KB8101.seq Check: 6904 from: 1 to: 4454
pF1KB8101 4454 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 620 1430 620
Size: 810 3644
AccI GT'mk_AC
Cuts at: 385 437 620 1492 385
Size: 52 183 872 3347
Fragments arranged by size:
3347 872 183 52
Acc65I G'GTAC_C
Cuts at: 1470 1470
Size: 4454
AclI AA'CG_TT
Cuts at: 4294 4294
Size: 4454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2711 3458 3726 2279
Size: 432 747 268 3007
Fragments arranged by size:
3007 747 432 268
AflIII A'CryG_T
Cuts at: 1106 1806 2910 3887 1106
Size: 700 1104 977 1673
Fragments arranged by size:
1673 1104 977 700
AgeI A'CCGG_T
Cuts at: 142 2842 142
Size: 2700 1754
AleI CACnn'nnGTG
Cuts at: 1174 1174
Size: 4454
AlwNI CAG_nnn'CTG
Cuts at: 282 1759 3326 282
Size: 1477 1567 1410
Fragments arranged by size:
1567 1477 1410
ApaI G_GGCC'C
Cuts at: 293 293
Size: 4454
ApaLI G'TGCA_C
Cuts at: 3224 3224
Size: 4454
ApoI r'AATT_y
Cuts at: 343 1458 3807 4063 343
Size: 1115 2349 256 734
Fragments arranged by size:
2349 1115 734 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4454
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
AvaI C'yCGr_G
Cuts at: 1474 1474
Size: 4454
BamHI G'GATC_C
Cuts at: 1479 1479
Size: 4454
BanI G'GyrC_C
Cuts at: 1470 1884 2132 2167 1470
Size: 414 248 35 3757
Fragments arranged by size:
3757 414 248 35
BanII G_rGCy'C
Cuts at: 238 293 775 1232 1274 1468 2498 238
Size: 55 482 457 42 194 1030 2194
Fragments arranged by size:
2194 1030 482 457 194 55 42
BbeI G_GCGC'C
Cuts at: 1888 2136 1888
Size: 248 4206
BciVI GTATCCnnnnn_n'
Cuts at: 1024 2352 3113 4454 1024
Size: 1328 761 1341 1024
Fragments arranged by size:
1341 1328 1024 761
BclI T'GATC_A
Cuts at: 1974 1974
Size: 4454
BfrBI ATG'CAT
Cuts at: 158 158
Size: 4454
BglII A'GATC_T
Cuts at: 452 1969 4006 452
Size: 1517 2037 900
Fragments arranged by size:
2037 1517 900
BlpI GC'TnA_GC
Cuts at: 1442 1566 1442
Size: 124 4330
Bme1580I G_kGCm'C
Cuts at: 293 2079 2172 3228 293
Size: 1786 93 1056 1519
Fragments arranged by size:
1786 1519 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1666 1747 2082 4226 1666
Size: 81 335 2144 1894
Fragments arranged by size:
2144 1894 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 255 2821 255
Size: 2566 1888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 304 1237 274
Size: 30 933 3491
Fragments arranged by size:
3491 933 30
Bpu10I CC'TnA_GC
Cuts at: 1018 1018
Size: 4454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1630 2509 3001 3299 736
Size: 894 879 492 298 1891
Fragments arranged by size:
1891 894 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 151 712 1174 4158 151
Size: 561 462 2984 447
Fragments arranged by size:
2984 561 462 447
BsaAI yAC'GTr
Cuts at: 1107 1807 1107
Size: 700 3754
BsaBI GATnn'nnATC
Cuts at: 6 1968 4005 6
Size: 1962 2037 455
Fragments arranged by size:
2037 1962 455
BsaHI Gr'CG_yC
Cuts at: 617 1046 1427 1885 2133 2835 4331 617
Size: 429 381 458 248 702 1496 740
Fragments arranged by size:
1496 740 702 458 429 381 248
BsaWI w'CCGG_w
Cuts at: 142 209 623 1643 2164 2842 3116 3263 3674
Size: 67 414 1020 521 678 274 147 411
Cuts at: 3674 142
Size: 922
Fragments arranged by size:
1020 922 678 521 414 411 274 147
67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 247 277 1036 1066 247
Size: 30 759 30 3635
Fragments arranged by size:
3635 759 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 228 271 228
Size: 43 4411
BseYI C'CCAG_C
Cuts at: 135 958 1879 2637 3214 3710 135
Size: 823 921 758 577 496 879
Fragments arranged by size:
921 879 823 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 184
Size: 4454
BsiEI CG_ry'CG
Cuts at: 104 1996 2042 3250 104
Size: 1892 46 1208 1308
Fragments arranged by size:
1892 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 238 1468 2246 2436 3228 238
Size: 1230 778 190 792 1464
Fragments arranged by size:
1464 1230 792 778 190
BsmI GAATG_Cn'
Cuts at: 160 160
Size: 4454
Bsp1286I G_dGCh'C
Cuts at: 238 293 775 1232 1274 1468 2079 2172 2246
Size: 55 482 457 42 194 611 93 74
Cuts at: 2246 2436 2498 3228 238
Size: 190 62 730 1464
Fragments arranged by size:
1464 730 611 482 457 194 190 93
74 62 55 42
BspEI T'CCGG_A
Cuts at: 3674 3674
Size: 4454
BspHI T'CATG_A
Cuts at: 3630 3630
Size: 4454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1354 1437 1504 1718 2020 2401 1354
Size: 83 67 214 302 381 3407
Fragments arranged by size:
3407 381 302 214 83 67
BsrBI CCG'CTC
Cuts at: 50 789 2746 2800 4284 4449 50
Size: 739 1957 54 1484 165 55
Fragments arranged by size:
1957 1484 739 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2366 2366
Size: 4454
BsrFI r'CCGG_y
Cuts at: 142 1209 2452 2633 2842 142
Size: 1067 1243 181 209 1754
Fragments arranged by size:
1754 1243 1067 209 181
BsrGI T'GTAC_A
Cuts at: 740 740
Size: 4454
BssSI C'ACGA_G
Cuts at: 2725 3083 2725
Size: 358 4096
BstAPI GCAn_nnn'nTGC
Cuts at: 1831 1831
Size: 4454
BstBI TT'CG_AA
Cuts at: 2815 2815
Size: 4454
BstXI CCAn_nnnn'nTGG
Cuts at: 501 501
Size: 4454
BstYI r'GATC_y
Cuts at: 1 452 1479 1946 1969 2304 2550 3562 3648
Size: 451 1027 467 23 335 246 1012 86
Cuts at: 3648 3660 4006 1
Size: 12 346 449
Fragments arranged by size:
1027 1012 467 451 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 386 386
Size: 4454
BtgI C'CryG_G
Cuts at: 107 1184 107
Size: 1077 3377
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2404 2585 2748 2404
Size: 181 163 4110
Fragments arranged by size:
4110 181 163
BtsI GCAGTG_nn'
Cuts at: 1697 1697
Size: 4454
ClaI AT'CG_AT
Cuts at: 653 653
Size: 4454
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1317 1352 1317
Size: 35 4419
DraI TTT'AAA
Cuts at: 1453 1453
Size: 4454
DraIII CAC_nnn'GTG
Cuts at: 1134 1134
Size: 4454
DrdI GACnn_nn'nnGTC
Cuts at: 2160 3018 2160
Size: 858 3596
EaeI y'GGCC_r
Cuts at: 663 990 2039 2213 2604 2631 3976 663
Size: 327 1049 174 391 27 1345 1141
Fragments arranged by size:
1345 1141 1049 391 327 174 27
EagI C'GGCC_G
Cuts at: 2039 2039
Size: 4454
EarI CTCTTCn'nnn_
Cuts at: 2477 2687 2477
Size: 210 4244
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2662 2972 3118 4264 2662
Size: 310 146 1146 2852
Fragments arranged by size:
2852 1146 310 146
EcoICRI GAG'CTC
Cuts at: 236 1466 236
Size: 1230 3224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 304 1237 2279 2711 3458 3726 274
Size: 30 933 1042 432 747 268 1002
Fragments arranged by size:
1042 1002 933 747 432 268 30
EcoO109I rG'GnC_Cy
Cuts at: 289 290 1593 289
Size: 1 1303 3150
Fragments arranged by size:
3150 1303 1
EcoRI G'AATT_C
Cuts at: 1458 1458
Size: 4454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4454
FspI TGC'GCA
Cuts at: 2235 2235
Size: 4454
HaeII r_GCGC'y
Cuts at: 525 679 1888 2136 3158 4137 525
Size: 154 1209 248 1022 979 842
Fragments arranged by size:
1209 1022 979 842 248 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1036 1068 2296 2326 2328 2358 1036
Size: 32 1228 30 2 30 3132
Fragments arranged by size:
3132 1228 32 30 30 2
HincII GTy'rAC
Cuts at: 330 591 726 1086 1493 330
Size: 261 135 360 407 3291
Fragments arranged by size:
3291 407 360 261 135
KasI G'GCGC_C
Cuts at: 1884 2132 1884
Size: 248 4206
KpnI G_GTAC'C
Cuts at: 1474 1474
Size: 4454
MluI A'CGCG_T
Cuts at: 3887 3887
Size: 4454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1205 3125 3309 1205
Size: 1920 184 2350
Fragments arranged by size:
2350 1920 184
MscI TGG'CCA
Cuts at: 665 992 2215 665
Size: 327 1223 2904
Fragments arranged by size:
2904 1223 327
MslI CAynn'nnrTG
Cuts at: 178 883 1105 1174 2443 2570 178
Size: 705 222 69 1269 127 2062
Fragments arranged by size:
2062 1269 705 222 127 69
MspA1I CmG'CkG
Cuts at: 366 935 945 1565 1879 2239 3252 3497 366
Size: 569 10 620 314 360 1013 245 1323
Fragments arranged by size:
1323 1013 620 569 360 314 245 10
NaeI GCC'GGC
Cuts at: 1211 2635 1211
Size: 1424 3030
NarI GG'CG_CC
Cuts at: 1885 2133 1885
Size: 248 4206
NcoI C'CATG_G
Cuts at: 107 107
Size: 4454
NgoMIV G'CCGG_C
Cuts at: 1209 2633 1209
Size: 1424 3030
NsiI A_TGCA'T
Cuts at: 160 160
Size: 4454
NspI r_CATG'y
Cuts at: 510 612 1507 2007 2914 510
Size: 102 895 500 907 2050
Fragments arranged by size:
2050 907 895 500 102
PasI CC'CwG_GG
Cuts at: 813 1143 813
Size: 330 4124
PciI A'CATG_T
Cuts at: 2910 2910
Size: 4454
PflMI CCAn_nnn'nTGG
Cuts at: 672 672
Size: 4454
PfoI T'CCnGG_A
Cuts at: 968 968
Size: 4454
PmeI GTTT'AAAC
Cuts at: 1453 1453
Size: 4454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4422
PspOMI G'GGCC_C
Cuts at: 289 289
Size: 4454
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 205 237 205
Size: 32 4422
PstI C_TGCA'G
Cuts at: 1501 2186 1501
Size: 685 3769
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4454
PvuII CAG'CTG
Cuts at: 366 945 1879 2239 366
Size: 579 934 360 2581
Fragments arranged by size:
2581 934 579 360
RsrII CG'GwC_CG
Cuts at: 972 2649 972
Size: 1677 2777
SacI G_AGCT'C
Cuts at: 238 1468 238
Size: 1230 3224
SalI G'TCGA_C
Cuts at: 1491 1491
Size: 4454
SapI GCTCTTCn'nnn_
Cuts at: 2477 2687 2477
Size: 210 4244
SbfI CC_TGCA'GG
Cuts at: 1501 1501
Size: 4454
ScaI AGT'ACT
Cuts at: 3971 3971
Size: 4454
SexAI A'CCwGG_T
Cuts at: 1079 1079
Size: 4454
SfcI C'TryA_G
Cuts at: 33 1497 2182 3175 3366 33
Size: 1464 685 993 191 1121
Fragments arranged by size:
1464 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1886 2134 1886
Size: 248 4206
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
SmaI CCC'GGG
Cuts at: 1476 1476
Size: 4454
SmlI C'TyrA_G
Cuts at: 715 1609 2524 3016 3278 715
Size: 894 915 492 262 1891
Fragments arranged by size:
1891 915 894 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4454
SphI G_CATG'C
Cuts at: 612 1507 2007 612
Size: 895 500 3059
Fragments arranged by size:
3059 895 500
StuI AGG'CCT
Cuts at: 484 484
Size: 4454
StyI C'CwwG_G
Cuts at: 107 485 1588 1942 107
Size: 378 1103 354 2619
Fragments arranged by size:
2619 1103 378 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2037 2170 2629 2838 2037
Size: 133 459 209 3653
Fragments arranged by size:
3653 459 209 133
TatI w'GTAC_w
Cuts at: 740 1151 3969 740
Size: 411 2818 1225
Fragments arranged by size:
2818 1225 411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3971 4003 3971
Size: 32 4422
Tth111I GACn'n_nGTC
Cuts at: 2251 2251
Size: 4454
XbaI T'CTAG_A
Cuts at: 1485 1485
Size: 4454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 249 348 1173 1949 249
Size: 99 825 776 2754
Fragments arranged by size:
2754 825 776 99
XmaI C'CCGG_G
Cuts at: 1474 1474
Size: 4454
ZraI GAC'GTC
Cuts at: 618 1428 618
Size: 810 3644
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BglI BmgBI BmtI BsiWI
BsmBI BssHII BstEII Bsu36I EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PmlI PpuMI PshAI PsiI PspXI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI