Restriction map for
pF1KB7994
(Circular) (Six-base) MAPSORT of: pF1KB7994.seq Check: 6144 from: 1 to: 4454
pF1KB7994 4454 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1492 1492
Size: 4454
Acc65I G'GTAC_C
Cuts at: 584 1470 584
Size: 886 3568
AclI AA'CG_TT
Cuts at: 4294 4294
Size: 4454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 872 2279 2711 3458 3726 865
Size: 7 1407 432 747 268 1593
Fragments arranged by size:
1593 1407 747 432 268 7
AflIII A'CryG_T
Cuts at: 1806 2910 3887 1806
Size: 1104 977 2373
Fragments arranged by size:
2373 1104 977
AgeI A'CCGG_T
Cuts at: 602 682 2842 602
Size: 80 2160 2214
Fragments arranged by size:
2214 2160 80
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1049 1049
Size: 4454
AlwNI CAG_nnn'CTG
Cuts at: 270 901 1151 1200 1759 3326 270
Size: 631 250 49 559 1567 1398
Fragments arranged by size:
1567 1398 631 559 250 49
ApaI G_GGCC'C
Cuts at: 1394 1394
Size: 4454
ApaLI G'TGCA_C
Cuts at: 348 799 3224 348
Size: 451 2425 1578
Fragments arranged by size:
2425 1578 451
ApoI r'AATT_y
Cuts at: 442 856 1458 3807 4063 442
Size: 414 602 2349 256 833
Fragments arranged by size:
2349 833 602 414 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4454
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
AvaI C'yCGr_G
Cuts at: 146 1474 146
Size: 1328 3126
BamHI G'GATC_C
Cuts at: 528 1479 528
Size: 951 3503
BanI G'GyrC_C
Cuts at: 457 584 1213 1470 1884 2132 2167 457
Size: 127 629 257 414 248 35 2744
Fragments arranged by size:
2744 629 414 257 248 127 35
BanII G_rGCy'C
Cuts at: 1394 1468 2498 1394
Size: 74 1030 3350
Fragments arranged by size:
3350 1030 74
BbeI G_GCGC'C
Cuts at: 1888 2136 1888
Size: 248 4206
BciVI GTATCCnnnnn_n'
Cuts at: 2352 3113 4454 2352
Size: 761 1341 2352
Fragments arranged by size:
2352 1341 761
BclI T'GATC_A
Cuts at: 467 1974 467
Size: 1507 2947
BfrBI ATG'CAT
Cuts at: 780 780
Size: 4454
BglI GCCn_nnn'nGGC
Cuts at: 651 651
Size: 4454
BglII A'GATC_T
Cuts at: 1969 4006 1969
Size: 2037 2417
BlpI GC'TnA_GC
Cuts at: 1566 1566
Size: 4454
Bme1580I G_kGCm'C
Cuts at: 264 352 460 803 1394 2079 2172 3228 264
Size: 88 108 343 591 685 93 1056 1490
Fragments arranged by size:
1490 1056 685 591 343 108 93 88
BmrI ACTGGGnnnn_n'
Cuts at: 380 1294 1666 1747 2082 4226 380
Size: 914 372 81 335 2144 608
Fragments arranged by size:
2144 914 608 372 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 4454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 439 960 253
Size: 186 521 3747
Fragments arranged by size:
3747 521 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 2509 3001 3299 1630
Size: 879 492 298 2785
Fragments arranged by size:
2785 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 415 4158 415
Size: 3743 711
BsaAI yAC'GTr
Cuts at: 1807 1807
Size: 4454
BsaBI GATnn'nnATC
Cuts at: 6 469 559 944 1968 4005 6
Size: 463 90 385 1024 2037 455
Fragments arranged by size:
2037 1024 463 455 385 90
BsaHI Gr'CG_yC
Cuts at: 121 1438 1885 2133 2835 4331 121
Size: 1317 447 248 702 1496 244
Fragments arranged by size:
1496 1317 702 447 248 244
BsaWI w'CCGG_w
Cuts at: 602 682 1643 2164 2842 3116 3263 3674 602
Size: 80 961 521 678 274 147 411 1382
Fragments arranged by size:
1382 961 678 521 411 274 147 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 145 175 238 268 592 622 145
Size: 30 63 30 324 30 3977
Fragments arranged by size:
3977 324 63 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 132 327 330 1076 132
Size: 195 3 746 3510
Fragments arranged by size:
3510 746 195 3
BseYI C'CCAG_C
Cuts at: 263 453 1879 2637 3214 3710 263
Size: 190 1426 758 577 496 1007
Fragments arranged by size:
1426 1007 758 577 496 190
BsiEI CG_ry'CG
Cuts at: 104 1996 2042 3250 104
Size: 1892 46 1208 1308
Fragments arranged by size:
1892 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 352 803 1468 2246 2436 3228 352
Size: 451 665 778 190 792 1578
Fragments arranged by size:
1578 792 778 665 451 190
BsmI GAATG_Cn'
Cuts at: 1026 1295 1026
Size: 269 4185
Bsp1286I G_dGCh'C
Cuts at: 264 352 460 803 1394 1468 2079 2172 2246
Size: 88 108 343 591 74 611 93 74
Cuts at: 2246 2436 2498 3228 264
Size: 190 62 730 1490
Fragments arranged by size:
1490 730 611 591 343 190 108 93
88 74 74 62
BspEI T'CCGG_A
Cuts at: 3674 3674
Size: 4454
BspHI T'CATG_A
Cuts at: 3630 3630
Size: 4454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1504 1718 2020 2401 1504
Size: 214 302 381 3557
Fragments arranged by size:
3557 381 302 214
BsrBI CCG'CTC
Cuts at: 50 185 2746 2800 4284 4449 50
Size: 135 2561 54 1484 165 55
Fragments arranged by size:
2561 1484 165 135 55 54
BsrDI GCAATG_nn'
Cuts at: 164 832 1310 2366 164
Size: 668 478 1056 2252
Fragments arranged by size:
2252 1056 668 478
BsrFI r'CCGG_y
Cuts at: 602 682 2452 2633 2842 602
Size: 80 1770 181 209 2214
Fragments arranged by size:
2214 1770 209 181 80
BssSI C'ACGA_G
Cuts at: 2725 3083 2725
Size: 358 4096
BstAPI GCAn_nnn'nTGC
Cuts at: 901 1831 901
Size: 930 3524
BstBI TT'CG_AA
Cuts at: 2815 2815
Size: 4454
BstYI r'GATC_y
Cuts at: 1 528 1479 1946 1969 2304 2550 3562 3648
Size: 527 951 467 23 335 246 1012 86
Cuts at: 3648 3660 4006 1
Size: 12 346 449
Fragments arranged by size:
1012 951 527 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 533 533
Size: 4454
BtgI C'CryG_G
Cuts at: 107 107
Size: 4454
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2404 2585 2748 2404
Size: 181 163 4110
Fragments arranged by size:
4110 181 163
BtsI GCAGTG_nn'
Cuts at: 324 899 1697 324
Size: 575 798 3081
Fragments arranged by size:
3081 798 575
DraI TTT'AAA
Cuts at: 1453 1453
Size: 4454
DrdI GACnn_nn'nnGTC
Cuts at: 2160 3018 2160
Size: 858 3596
EaeI y'GGCC_r
Cuts at: 398 756 1307 2039 2213 2604 2631 3976 398
Size: 358 551 732 174 391 27 1345 876
Fragments arranged by size:
1345 876 732 551 391 358 174 27
EagI C'GGCC_G
Cuts at: 2039 2039
Size: 4454
EarI CTCTTCn'nnn_
Cuts at: 2477 2687 2477
Size: 210 4244
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2662 2972 3118 4264 2662
Size: 310 146 1146 2852
Fragments arranged by size:
2852 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1466 1466
Size: 4454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 439 865 872 960 2279 2711 3458 3726
Size: 186 426 7 88 1319 432 747 268
Cuts at: 3726 253
Size: 981
Fragments arranged by size:
1319 981 747 432 426 268 186 88
7
EcoO109I rG'GnC_Cy
Cuts at: 1390 1391 1593 1390
Size: 1 202 4251
Fragments arranged by size:
4251 202 1
EcoRI G'AATT_C
Cuts at: 1458 1458
Size: 4454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4454
FspI TGC'GCA
Cuts at: 2235 2235
Size: 4454
HaeII r_GCGC'y
Cuts at: 1888 2136 3158 4137 1888
Size: 248 1022 979 2205
Fragments arranged by size:
2205 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 1123 1155 2296 2326 2328 2358 238
Size: 32 853 32 1141 30 2 30 2334
Fragments arranged by size:
2334 1141 853 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1493 1493
Size: 4454
KasI G'GCGC_C
Cuts at: 1884 2132 1884
Size: 248 4206
KpnI G_GTAC'C
Cuts at: 588 1474 588
Size: 886 3568
MluI A'CGCG_T
Cuts at: 3887 3887
Size: 4454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 523 3125 3309 523
Size: 2602 184 1668
Fragments arranged by size:
2602 1668 184
MscI TGG'CCA
Cuts at: 400 758 2215 400
Size: 358 1457 2639
Fragments arranged by size:
2639 1457 358
MslI CAynn'nnrTG
Cuts at: 463 508 1031 2443 2570 463
Size: 45 523 1412 127 2347
Fragments arranged by size:
2347 1412 523 127 45
MspA1I CmG'CkG
Cuts at: 1565 1879 2239 3252 3497 1565
Size: 314 360 1013 245 2522
Fragments arranged by size:
2522 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2635 2635
Size: 4454
NarI GG'CG_CC
Cuts at: 1885 2133 1885
Size: 248 4206
NcoI C'CATG_G
Cuts at: 107 107
Size: 4454
NgoMIV G'CCGG_C
Cuts at: 2633 2633
Size: 4454
NsiI A_TGCA'T
Cuts at: 782 782
Size: 4454
NspI r_CATG'y
Cuts at: 1362 1507 2007 2914 1362
Size: 145 500 907 2902
Fragments arranged by size:
2902 907 500 145
PasI CC'CwG_GG
Cuts at: 270 270
Size: 4454
PciI A'CATG_T
Cuts at: 2910 2910
Size: 4454
PflMI CCAn_nnn'nTGG
Cuts at: 270 453 270
Size: 183 4271
PfoI T'CCnGG_A
Cuts at: 965 965
Size: 4454
PmeI GTTT'AAAC
Cuts at: 1453 1453
Size: 4454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4422
PspOMI G'GGCC_C
Cuts at: 1390 1390
Size: 4454
PstI C_TGCA'G
Cuts at: 415 1501 2186 415
Size: 1086 685 2683
Fragments arranged by size:
2683 1086 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4454
PvuII CAG'CTG
Cuts at: 1879 2239 1879
Size: 360 4094
RsrII CG'GwC_CG
Cuts at: 2649 2649
Size: 4454
SacI G_AGCT'C
Cuts at: 1468 1468
Size: 4454
SalI G'TCGA_C
Cuts at: 1491 1491
Size: 4454
SapI GCTCTTCn'nnn_
Cuts at: 2477 2687 2477
Size: 210 4244
SbfI CC_TGCA'GG
Cuts at: 1501 1501
Size: 4454
ScaI AGT'ACT
Cuts at: 3971 3971
Size: 4454
SfcI C'TryA_G
Cuts at: 33 411 1497 2182 3175 3366 33
Size: 378 1086 685 993 191 1121
Fragments arranged by size:
1121 1086 993 685 378 191
SfoI GGC'GCC
Cuts at: 1886 2134 1886
Size: 248 4206
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
SmaI CCC'GGG
Cuts at: 148 1476 148
Size: 1328 3126
SmlI C'TyrA_G
Cuts at: 1609 2524 3016 3278 1609
Size: 915 492 262 2785
Fragments arranged by size:
2785 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4454
SphI G_CATG'C
Cuts at: 1507 2007 1507
Size: 500 3954
StyI C'CwwG_G
Cuts at: 107 425 793 1588 1942 107
Size: 318 368 795 354 2619
Fragments arranged by size:
2619 795 368 354 318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 1059 1205 1254 2037 2170 2629 2838 262
Size: 797 146 49 783 133 459 209 1878
Fragments arranged by size:
1878 797 783 459 209 146 133 49
TatI w'GTAC_w
Cuts at: 542 3969 542
Size: 3427 1027
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 592 624 1180 1212 3971 4003 592
Size: 32 556 32 2759 32 1043
Fragments arranged by size:
2759 1043 556 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 562 2251 562
Size: 1689 2765
XbaI T'CTAG_A
Cuts at: 1485 1485
Size: 4454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 393 741 1949 393
Size: 348 1208 2898
Fragments arranged by size:
2898 1208 348
XmaI C'CCGG_G
Cuts at: 146 1474 146
Size: 1328 3126
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI