Restriction map for
pF1KB7988
(Circular) (Six-base) MAPSORT of: pF1KB7988.seq Check: 9986 from: 1 to: 4088
pF1KB7988 4088 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1126 1126
Size: 4088
Acc65I G'GTAC_C
Cuts at: 1104 1104
Size: 4088
AclI AA'CG_TT
Cuts at: 3928 3928
Size: 4088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1913 2345 3092 3360 1913
Size: 432 747 268 2641
Fragments arranged by size:
2641 747 432 268
AflIII A'CryG_T
Cuts at: 608 1010 1440 2544 3521 608
Size: 402 430 1104 977 1175
Fragments arranged by size:
1175 1104 977 430 402
AgeI A'CCGG_T
Cuts at: 2476 2476
Size: 4088
AleI CACnn'nnGTG
Cuts at: 301 301
Size: 4088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 649 649
Size: 4088
AlwNI CAG_nnn'CTG
Cuts at: 813 1393 2960 813
Size: 580 1567 1941
Fragments arranged by size:
1941 1567 580
ApaI G_GGCC'C
Cuts at: 365 365
Size: 4088
ApaLI G'TGCA_C
Cuts at: 2858 2858
Size: 4088
ApoI r'AATT_y
Cuts at: 1092 3441 3697 1092
Size: 2349 256 1483
Fragments arranged by size:
2349 1483 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4088
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
AvaI C'yCGr_G
Cuts at: 220 283 358 545 1108 220
Size: 63 75 187 563 3200
Fragments arranged by size:
3200 563 187 75 63
BamHI G'GATC_C
Cuts at: 1113 1113
Size: 4088
BanI G'GyrC_C
Cuts at: 243 936 1021 1104 1518 1766 1801 243
Size: 693 85 83 414 248 35 2530
Fragments arranged by size:
2530 693 414 248 85 83 35
BanII G_rGCy'C
Cuts at: 214 310 365 1102 2132 214
Size: 96 55 737 1030 2170
Fragments arranged by size:
2170 1030 737 96 55
BbeI G_GCGC'C
Cuts at: 247 1522 1770 247
Size: 1275 248 2565
Fragments arranged by size:
2565 1275 248
BbsI GAAGACnn'nnnn_
Cuts at: 604 604
Size: 4088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1011 1045 90
Size: 34 887 34 3133
Fragments arranged by size:
3133 887 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1986 2747 4088 1986
Size: 761 1341 1986
Fragments arranged by size:
1986 1341 761
BclI T'GATC_A
Cuts at: 1608 1608
Size: 4088
BglI GCCn_nnn'nGGC
Cuts at: 325 392 325
Size: 67 4021
BglII A'GATC_T
Cuts at: 1603 3640 1603
Size: 2037 2051
BlpI GC'TnA_GC
Cuts at: 1200 1200
Size: 4088
Bme1580I G_kGCm'C
Cuts at: 365 941 1024 1713 1806 2862 365
Size: 576 83 689 93 1056 1591
Fragments arranged by size:
1591 1056 689 576 93 83
BmgBI CAC'GTC
Cuts at: 1011 1011
Size: 4088
BmrI ACTGGGnnnn_n'
Cuts at: 253 813 1300 1381 1716 3860 253
Size: 560 487 81 335 2144 481
Fragments arranged by size:
2144 560 487 481 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 4088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 2143 2635 2933 1264
Size: 879 492 298 2419
Fragments arranged by size:
2419 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3792 3792
Size: 4088
BsaAI yAC'GTr
Cuts at: 1441 1441
Size: 4088
BsaBI GATnn'nnATC
Cuts at: 6 1602 3639 6
Size: 1596 2037 455
Fragments arranged by size:
2037 1596 455
BsaHI Gr'CG_yC
Cuts at: 134 244 1519 1767 2469 3965 134
Size: 110 1275 248 702 1496 257
Fragments arranged by size:
1496 1275 702 257 248 110
BsaWI w'CCGG_w
Cuts at: 428 970 1277 1798 2476 2750 2897 3308 428
Size: 542 307 521 678 274 147 411 1208
Fragments arranged by size:
1208 678 542 521 411 307 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 483 526 483
Size: 43 4045
BseYI C'CCAG_C
Cuts at: 198 382 731 783 958 980 1513 2271 2848
Size: 184 349 52 175 22 533 758 577
Cuts at: 2848 3344 198
Size: 496 942
Fragments arranged by size:
942 758 577 533 496 349 184 175
52 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 843 571
Size: 272 3816
BsiEI CG_ry'CG
Cuts at: 104 743 836 1630 1676 2884 104
Size: 639 93 794 46 1208 1308
Fragments arranged by size:
1308 1208 794 639 93 46
BsiHKAI G_wGCw'C
Cuts at: 709 1061 1102 1880 2070 2862 709
Size: 352 41 778 190 792 1935
Fragments arranged by size:
1935 792 778 352 190 41
BsmBI CGTCTCn'nnnn_
Cuts at: 972 972
Size: 4088
Bsp1286I G_dGCh'C
Cuts at: 214 310 365 709 941 1024 1061 1102 1713
Size: 96 55 344 232 83 37 41 611
Cuts at: 1713 1806 1880 2070 2132 2862 214
Size: 93 74 190 62 730 1440
Fragments arranged by size:
1440 730 611 344 232 190 96 93
83 74 62 55 41 37
BspEI T'CCGG_A
Cuts at: 3308 3308
Size: 4088
BspHI T'CATG_A
Cuts at: 3264 3264
Size: 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 543 1138 1352 1654 2035 543
Size: 595 214 302 381 2596
Fragments arranged by size:
2596 595 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2380 2434 3918 4083 50
Size: 2330 54 1484 165 55
Fragments arranged by size:
2330 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2000 2000
Size: 4088
BsrFI r'CCGG_y
Cuts at: 391 777 835 2086 2267 2476 391
Size: 386 58 1251 181 209 2003
Fragments arranged by size:
2003 1251 386 209 181 58
BssSI C'ACGA_G
Cuts at: 2359 2717 2359
Size: 358 3730
BstAPI GCAn_nnn'nTGC
Cuts at: 1465 1465
Size: 4088
BstBI TT'CG_AA
Cuts at: 2449 2449
Size: 4088
BstXI CCAn_nnnn'nTGG
Cuts at: 855 873 855
Size: 18 4070
BstYI r'GATC_y
Cuts at: 1 1113 1580 1603 1938 2184 3196 3282 3294
Size: 1112 467 23 335 246 1012 86 12
Cuts at: 3294 3640 1
Size: 346 449
Fragments arranged by size:
1112 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 689 800 854 689
Size: 111 54 3923
Fragments arranged by size:
3923 111 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1014 2038 2219 2382 1014
Size: 1024 181 163 2720
Fragments arranged by size:
2720 1024 181 163
BtsI GCAGTG_nn'
Cuts at: 520 628 1331 520
Size: 108 703 3277
Fragments arranged by size:
3277 703 108
DraI TTT'AAA
Cuts at: 1087 1087
Size: 4088
DrdI GACnn_nn'nnGTC
Cuts at: 1794 2652 1794
Size: 858 3230
EaeI y'GGCC_r
Cuts at: 393 740 833 1673 1847 2238 2265 3610 393
Size: 347 93 840 174 391 27 1345 871
Fragments arranged by size:
1345 871 840 391 347 174 93 27
EagI C'GGCC_G
Cuts at: 740 833 1673 740
Size: 93 840 3155
Fragments arranged by size:
3155 840 93
EarI CTCTTCn'nnn_
Cuts at: 2111 2321 2111
Size: 210 3878
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 830 1030 2296 2606 2752 3898 830
Size: 200 1266 310 146 1146 1020
Fragments arranged by size:
1266 1146 1020 310 200 146
EcoICRI GAG'CTC
Cuts at: 1100 1100
Size: 4088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1913 2345 3092 3360 1913
Size: 432 747 268 2641
Fragments arranged by size:
2641 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 237 362 1227 237
Size: 125 865 3098
Fragments arranged by size:
3098 865 125
EcoRI G'AATT_C
Cuts at: 1092 1092
Size: 4088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 4088
FspI TGC'GCA
Cuts at: 1869 1869
Size: 4088
HaeII r_GCGC'y
Cuts at: 247 338 426 784 1054 1522 1770 2792 3771
Size: 91 88 358 270 468 248 1022 979
Cuts at: 3771 247
Size: 564
Fragments arranged by size:
1022 979 564 468 358 270 248 91
88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 539 571 1930 1960 1962 1992 539
Size: 32 1359 30 2 30 2635
Fragments arranged by size:
2635 1359 32 30 30 2
HincII GTy'rAC
Cuts at: 1127 1127
Size: 4088
KasI G'GCGC_C
Cuts at: 243 1518 1766 243
Size: 1275 248 2565
Fragments arranged by size:
2565 1275 248
KpnI G_GTAC'C
Cuts at: 1108 1108
Size: 4088
MluI A'CGCG_T
Cuts at: 3521 3521
Size: 4088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 239 443 890 908 2759 2943 239
Size: 204 447 18 1851 184 1384
Fragments arranged by size:
1851 1384 447 204 184 18
MscI TGG'CCA
Cuts at: 1849 1849
Size: 4088
MslI CAynn'nnrTG
Cuts at: 301 502 2077 2204 301
Size: 201 1575 127 2185
Fragments arranged by size:
2185 1575 201 127
MspA1I CmG'CkG
Cuts at: 679 731 765 1199 1513 1873 2886 3131 679
Size: 52 34 434 314 360 1013 245 1636
Fragments arranged by size:
1636 1013 434 360 314 245 52 34
NaeI GCC'GGC
Cuts at: 393 779 2269 393
Size: 386 1490 2212
Fragments arranged by size:
2212 1490 386
NarI GG'CG_CC
Cuts at: 244 1519 1767 244
Size: 1275 248 2565
Fragments arranged by size:
2565 1275 248
NcoI C'CATG_G
Cuts at: 689 800 854 689
Size: 111 54 3923
Fragments arranged by size:
3923 111 54
NgoMIV G'CCGG_C
Cuts at: 391 777 2267 391
Size: 386 1490 2212
Fragments arranged by size:
2212 1490 386
NotI GC'GGCC_GC
Cuts at: 740 740
Size: 4088
NspI r_CATG'y
Cuts at: 1141 1641 2548 1141
Size: 500 907 2681
Fragments arranged by size:
2681 907 500
PasI CC'CwG_GG
Cuts at: 366 366
Size: 4088
PciI A'CATG_T
Cuts at: 2544 2544
Size: 4088
PfoI T'CCnGG_A
Cuts at: 128 128
Size: 4088
PmeI GTTT'AAAC
Cuts at: 1087 1087
Size: 4088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 4056
PpuMI rG'GwC_Cy
Cuts at: 237 237
Size: 4088
PspOMI G'GGCC_C
Cuts at: 361 361
Size: 4088
PstI C_TGCA'G
Cuts at: 902 991 1135 1820 902
Size: 89 144 685 3170
Fragments arranged by size:
3170 685 144 89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4088
PvuII CAG'CTG
Cuts at: 765 1513 1873 765
Size: 748 360 2980
Fragments arranged by size:
2980 748 360
RsrII CG'GwC_CG
Cuts at: 838 2283 838
Size: 1445 2643
SacI G_AGCT'C
Cuts at: 1102 1102
Size: 4088
SalI G'TCGA_C
Cuts at: 1125 1125
Size: 4088
SapI GCTCTTCn'nnn_
Cuts at: 2111 2321 2111
Size: 210 3878
SbfI CC_TGCA'GG
Cuts at: 1135 1135
Size: 4088
ScaI AGT'ACT
Cuts at: 3605 3605
Size: 4088
SfcI C'TryA_G
Cuts at: 33 898 987 1131 1816 2809 3000 33
Size: 865 89 144 685 993 191 1121
Fragments arranged by size:
1121 993 865 685 191 144 89
SfoI GGC'GCC
Cuts at: 245 1520 1768 245
Size: 1275 248 2565
Fragments arranged by size:
2565 1275 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
SgrAI Cr'CCGG_yG
Cuts at: 777 777
Size: 4088
SmaI CCC'GGG
Cuts at: 222 285 360 1110 222
Size: 63 75 750 3200
Fragments arranged by size:
3200 750 75 63
SmlI C'TyrA_G
Cuts at: 1243 2158 2650 2912 1243
Size: 915 492 262 2419
Fragments arranged by size:
2419 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4088
SphI G_CATG'C
Cuts at: 1141 1641 1141
Size: 500 3588
SrfI GCCC'GGGC
Cuts at: 360 360
Size: 4088
StuI AGG'CCT
Cuts at: 647 647
Size: 4088
StyI C'CwwG_G
Cuts at: 689 800 854 1222 1576 689
Size: 111 54 368 354 3201
Fragments arranged by size:
3201 368 354 111 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 553 1671 1804 2263 2472 553
Size: 1118 133 459 209 2169
Fragments arranged by size:
2169 1118 459 209 133
TatI w'GTAC_w
Cuts at: 3603 3603
Size: 4088
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 4056
Tth111I GACn'n_nGTC
Cuts at: 540 1885 540
Size: 1345 2743
XbaI T'CTAG_A
Cuts at: 1119 1119
Size: 4088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1583 1583
Size: 4088
XmaI C'CCGG_G
Cuts at: 220 283 358 1108 220
Size: 63 75 750 3200
Fragments arranged by size:
3200 750 75 63
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI BpmI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SspI SwaI XhoI XmnI ZraI