Restriction map for
pF1KB7979
(Circular) (Six-base) MAPSORT of: pF1KB7979.seq Check: 4107 from: 1 to: 4409
pF1KB7979 4409 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 527 1447 527
Size: 920 3489
Acc65I G'GTAC_C
Cuts at: 716 1425 716
Size: 709 3700
AclI AA'CG_TT
Cuts at: 4249 4249
Size: 4409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 2234 2666 3413 3681 151
Size: 2083 432 747 268 879
Fragments arranged by size:
2083 879 747 432 268
AflIII A'CryG_T
Cuts at: 1202 1761 2865 3842 1202
Size: 559 1104 977 1769
Fragments arranged by size:
1769 1104 977 559
AgeI A'CCGG_T
Cuts at: 2797 2797
Size: 4409
AlwNI CAG_nnn'CTG
Cuts at: 1155 1158 1347 1714 3281 1155
Size: 3 189 367 1567 2283
Fragments arranged by size:
2283 1567 367 189 3
ApaLI G'TGCA_C
Cuts at: 3179 3179
Size: 4409
ApoI r'AATT_y
Cuts at: 499 1413 3762 4018 499
Size: 914 2349 256 890
Fragments arranged by size:
2349 914 890 256
AseI AT'TA_AT
Cuts at: 20 733 20
Size: 713 3696
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
AvaI C'yCGr_G
Cuts at: 1429 1429
Size: 4409
BamHI G'GATC_C
Cuts at: 1434 1434
Size: 4409
BanI G'GyrC_C
Cuts at: 716 907 1425 1839 2087 2122 716
Size: 191 518 414 248 35 3003
Fragments arranged by size:
3003 518 414 248 191 35
BanII G_rGCy'C
Cuts at: 1423 2453 1423
Size: 1030 3379
BbeI G_GCGC'C
Cuts at: 1843 2091 1843
Size: 248 4161
BbsI GAAGACnn'nnnn_
Cuts at: 221 221
Size: 4409
BbvCI CC'TCA_GC
Cuts at: 1255 1255
Size: 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 361 395 878 912 361
Size: 34 483 34 3858
Fragments arranged by size:
3858 483 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2307 3068 4409 2307
Size: 761 1341 2307
Fragments arranged by size:
2307 1341 761
BclI T'GATC_A
Cuts at: 960 1929 960
Size: 969 3440
BglI GCCn_nnn'nGGC
Cuts at: 901 1259 901
Size: 358 4051
BglII A'GATC_T
Cuts at: 1924 3961 1924
Size: 2037 2372
BlpI GC'TnA_GC
Cuts at: 1521 1521
Size: 4409
Bme1580I G_kGCm'C
Cuts at: 910 2034 2127 3183 910
Size: 1124 93 1056 2136
Fragments arranged by size:
2136 1124 1056 93
BmgBI CAC'GTC
Cuts at: 1026 1026
Size: 4409
BmrI ACTGGGnnnn_n'
Cuts at: 489 1621 1702 2037 4181 489
Size: 1132 81 335 2144 717
Fragments arranged by size:
2144 1132 717 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1324 463
Size: 861 3548
Bpu10I CC'TnA_GC
Cuts at: 1255 1255
Size: 4409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1585 2464 2956 3254 1585
Size: 879 492 298 2740
Fragments arranged by size:
2740 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4113 4113
Size: 4409
BsaAI yAC'GTr
Cuts at: 174 1762 174
Size: 1588 2821
BsaBI GATnn'nnATC
Cuts at: 6 1923 3960 6
Size: 1917 2037 455
Fragments arranged by size:
2037 1917 455
BsaHI Gr'CG_yC
Cuts at: 839 1840 2088 2790 4286 839
Size: 1001 248 702 1496 962
Fragments arranged by size:
1496 1001 962 702 248
BsaWI w'CCGG_w
Cuts at: 1598 2119 2797 3071 3218 3629 1598
Size: 521 678 274 147 411 2378
Fragments arranged by size:
2378 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 544
Size: 4409
BseYI C'CCAG_C
Cuts at: 427 1834 2592 3169 3665 427
Size: 1407 758 577 496 1171
Fragments arranged by size:
1407 1171 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 337
Size: 4409
BsiEI CG_ry'CG
Cuts at: 104 1263 1951 1997 3205 104
Size: 1159 688 46 1208 1308
Fragments arranged by size:
1308 1208 1159 688 46
BsiHKAI G_wGCw'C
Cuts at: 1423 2201 2391 3183 1423
Size: 778 190 792 2649
Fragments arranged by size:
2649 792 778 190
BsmI GAATG_Cn'
Cuts at: 975 975
Size: 4409
Bsp1286I G_dGCh'C
Cuts at: 910 1423 2034 2127 2201 2391 2453 3183 910
Size: 513 611 93 74 190 62 730 2136
Fragments arranged by size:
2136 730 611 513 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3629 3629
Size: 4409
BspHI T'CATG_A
Cuts at: 3585 3585
Size: 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 498 704 1459 1673 1975 2356 498
Size: 206 755 214 302 381 2551
Fragments arranged by size:
2551 755 381 302 214 206
BsrBI CCG'CTC
Cuts at: 50 2701 2755 4239 4404 50
Size: 2651 54 1484 165 55
Fragments arranged by size:
2651 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2321 2321
Size: 4409
BsrFI r'CCGG_y
Cuts at: 2407 2588 2797 2407
Size: 181 209 4019
Fragments arranged by size:
4019 209 181
BssSI C'ACGA_G
Cuts at: 2680 3038 2680
Size: 358 4051
BstAPI GCAn_nnn'nTGC
Cuts at: 1786 1786
Size: 4409
BstBI TT'CG_AA
Cuts at: 2770 2770
Size: 4409
BstEII G'GTnAC_C
Cuts at: 792 792
Size: 4409
BstXI CCAn_nnnn'nTGG
Cuts at: 933 933
Size: 4409
BstYI r'GATC_y
Cuts at: 1 1434 1901 1924 2259 2505 3517 3603 3615
Size: 1433 467 23 335 246 1012 86 12
Cuts at: 3615 3961 1
Size: 346 449
Fragments arranged by size:
1433 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 528 528
Size: 4409
BtgI C'CryG_G
Cuts at: 107 119 504 639 107
Size: 12 385 135 3877
Fragments arranged by size:
3877 385 135 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2359 2540 2703 2359
Size: 181 163 4065
Fragments arranged by size:
4065 181 163
BtsI GCAGTG_nn'
Cuts at: 200 438 1364 1652 200
Size: 238 926 288 2957
Fragments arranged by size:
2957 926 288 238
ClaI AT'CG_AT
Cuts at: 371 371
Size: 4409
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4409
DrdI GACnn_nn'nnGTC
Cuts at: 595 2115 2973 595
Size: 1520 858 2031
Fragments arranged by size:
2031 1520 858
EaeI y'GGCC_r
Cuts at: 152 726 1260 1994 2168 2559 2586 3931 152
Size: 574 534 734 174 391 27 1345 630
Fragments arranged by size:
1345 734 630 574 534 391 174 27
EagI C'GGCC_G
Cuts at: 1260 1994 1260
Size: 734 3675
EarI CTCTTCn'nnn_
Cuts at: 443 1237 1370 2432 2642 443
Size: 794 133 1062 210 2210
Fragments arranged by size:
2210 1062 794 210 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2617 2927 3073 4219 2617
Size: 310 146 1146 2807
Fragments arranged by size:
2807 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1421 1421
Size: 4409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 463 1324 2234 2666 3413 3681 151
Size: 312 861 910 432 747 268 879
Fragments arranged by size:
910 879 861 747 432 312 268
EcoNI CCTnn'n_nnAGG
Cuts at: 133 133
Size: 4409
EcoO109I rG'GnC_Cy
Cuts at: 1251 1266 1311 1344 1548 1251
Size: 15 45 33 204 4112
Fragments arranged by size:
4112 204 45 33 15
EcoRI G'AATT_C
Cuts at: 499 1413 499
Size: 914 3495
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4409
FspI TGC'GCA
Cuts at: 2190 2190
Size: 4409
HaeII r_GCGC'y
Cuts at: 142 1843 2091 3113 4092 142
Size: 1701 248 1022 979 459
Fragments arranged by size:
1701 1022 979 459 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1113 1145 2251 2281 2283 2313 1113
Size: 32 1106 30 2 30 3209
Fragments arranged by size:
3209 1106 32 30 30 2
HincII GTy'rAC
Cuts at: 600 1448 600
Size: 848 3561
HindIII A'AGCT_T
Cuts at: 1387 1387
Size: 4409
KasI G'GCGC_C
Cuts at: 1839 2087 1839
Size: 248 4161
KpnI G_GTAC'C
Cuts at: 720 1429 720
Size: 709 3700
MluI A'CGCG_T
Cuts at: 3842 3842
Size: 4409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3080 3264 3080
Size: 184 4225
MscI TGG'CCA
Cuts at: 728 2170 728
Size: 1442 2967
MslI CAynn'nnrTG
Cuts at: 669 931 980 2398 2525 669
Size: 262 49 1418 127 2553
Fragments arranged by size:
2553 1418 262 127 49
MspA1I CmG'CkG
Cuts at: 411 496 1155 1259 1520 1834 2194 3207 3452
Size: 85 659 104 261 314 360 1013 245
Cuts at: 3452 411
Size: 1368
Fragments arranged by size:
1368 1013 659 360 314 261 245 104
85
NaeI GCC'GGC
Cuts at: 2590 2590
Size: 4409
NarI GG'CG_CC
Cuts at: 1840 2088 1840
Size: 248 4161
NcoI C'CATG_G
Cuts at: 107 504 107
Size: 397 4012
NgoMIV G'CCGG_C
Cuts at: 2588 2588
Size: 4409
NspI r_CATG'y
Cuts at: 386 1462 1962 2869 386
Size: 1076 500 907 1926
Fragments arranged by size:
1926 1076 907 500
PciI A'CATG_T
Cuts at: 2865 2865
Size: 4409
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4409
PmlI CAC'GTG
Cuts at: 174 174
Size: 4409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 4377
PpuMI rG'GwC_Cy
Cuts at: 1266 1266
Size: 4409
PstI C_TGCA'G
Cuts at: 495 901 1080 1456 2141 495
Size: 406 179 376 685 2763
Fragments arranged by size:
2763 685 406 376 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4409
PvuII CAG'CTG
Cuts at: 411 496 1155 1834 2194 411
Size: 85 659 679 360 2626
Fragments arranged by size:
2626 679 659 360 85
RsrII CG'GwC_CG
Cuts at: 2604 2604
Size: 4409
SacI G_AGCT'C
Cuts at: 1423 1423
Size: 4409
SalI G'TCGA_C
Cuts at: 1446 1446
Size: 4409
SapI GCTCTTCn'nnn_
Cuts at: 1237 2432 2642 1237
Size: 1195 210 3004
Fragments arranged by size:
3004 1195 210
SbfI CC_TGCA'GG
Cuts at: 901 1456 901
Size: 555 3854
ScaI AGT'ACT
Cuts at: 3926 3926
Size: 4409
SfcI C'TryA_G
Cuts at: 33 491 897 1076 1452 2137 3130 3321 33
Size: 458 406 179 376 685 993 191 1121
Fragments arranged by size:
1121 993 685 458 406 376 191 179
SfiI GGCCn_nnn'nGGCC
Cuts at: 1259 1259
Size: 4409
SfoI GGC'GCC
Cuts at: 1841 2089 1841
Size: 248 4161
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
SmaI CCC'GGG
Cuts at: 1431 1431
Size: 4409
SmlI C'TyrA_G
Cuts at: 1564 2479 2971 3233 1564
Size: 915 492 262 2740
Fragments arranged by size:
2740 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4409
SphI G_CATG'C
Cuts at: 386 1462 1962 386
Size: 1076 500 2833
Fragments arranged by size:
2833 1076 500
SspI AAT'ATT
Cuts at: 258 258
Size: 4409
StyI C'CwwG_G
Cuts at: 107 504 1543 1897 107
Size: 397 1039 354 2619
Fragments arranged by size:
2619 1039 397 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1992 2125 2584 2793 1992
Size: 133 459 209 3608
Fragments arranged by size:
3608 459 209 133
TatI w'GTAC_w
Cuts at: 1058 3924 1058
Size: 2866 1543
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 4377
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4409
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 646 1904 646
Size: 1258 3151
XmaI C'CCGG_G
Cuts at: 1429 1429
Size: 4409
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII Bsu36I CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI