Restriction map for
pF1KB7972
(Circular) (Six-base) MAPSORT of: pF1KB7972.seq Check: 3642 from: 1 to: 4346
pF1KB7972 4346 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1186 1384 1186
Size: 198 4148
Acc65I G'GTAC_C
Cuts at: 1362 1362
Size: 4346
AclI AA'CG_TT
Cuts at: 4186 4186
Size: 4346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 2171 2603 3350 3618 134
Size: 2037 432 747 268 862
Fragments arranged by size:
2037 862 747 432 268
AflIII A'CryG_T
Cuts at: 136 1062 1698 2802 3779 136
Size: 926 636 1104 977 703
Fragments arranged by size:
1104 977 926 703 636
AgeI A'CCGG_T
Cuts at: 2734 2734
Size: 4346
AlwNI CAG_nnn'CTG
Cuts at: 1651 3218 1651
Size: 1567 2779
ApaLI G'TGCA_C
Cuts at: 3116 3116
Size: 4346
ApoI r'AATT_y
Cuts at: 615 796 1300 1350 3699 3955 615
Size: 181 504 50 2349 256 1006
Fragments arranged by size:
2349 1006 504 256 181 50
AseI AT'TA_AT
Cuts at: 20 20
Size: 4346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
AvaI C'yCGr_G
Cuts at: 211 1366 211
Size: 1155 3191
BamHI G'GATC_C
Cuts at: 456 1371 456
Size: 915 3431
BanI G'GyrC_C
Cuts at: 241 1362 1776 2024 2059 241
Size: 1121 414 248 35 2528
Fragments arranged by size:
2528 1121 414 248 35
BanII G_rGCy'C
Cuts at: 1360 2390 1360
Size: 1030 3316
BbeI G_GCGC'C
Cuts at: 245 1780 2028 245
Size: 1535 248 2563
Fragments arranged by size:
2563 1535 248
BbvCI CC'TCA_GC
Cuts at: 295 406 295
Size: 111 4235
BciVI GTATCCnnnnn_n'
Cuts at: 1136 2244 3005 4346 1136
Size: 1108 761 1341 1136
Fragments arranged by size:
1341 1136 1108 761
BclI T'GATC_A
Cuts at: 1866 1866
Size: 4346
BglI GCCn_nnn'nGGC
Cuts at: 128 364 128
Size: 236 4110
BglII A'GATC_T
Cuts at: 1861 3898 1861
Size: 2037 2309
BlpI GC'TnA_GC
Cuts at: 401 1458 401
Size: 1057 3289
Bme1580I G_kGCm'C
Cuts at: 1971 2064 3120 1971
Size: 93 1056 3197
Fragments arranged by size:
3197 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 160 1030 1303 1558 1639 1974 4118 160
Size: 870 273 255 81 335 2144 388
Fragments arranged by size:
2144 870 388 335 273 255 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 4346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 766 409
Size: 357 3989
Bpu10I CC'TnA_GC
Cuts at: 295 406 824 295
Size: 111 418 3817
Fragments arranged by size:
3817 418 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1522 2401 2893 3191 463
Size: 1059 879 492 298 1618
Fragments arranged by size:
1618 1059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4050 4050
Size: 4346
BsaAI yAC'GTr
Cuts at: 137 1699 137
Size: 1562 2784
BsaBI GATnn'nnATC
Cuts at: 6 1860 3897 6
Size: 1854 2037 455
Fragments arranged by size:
2037 1854 455
BsaHI Gr'CG_yC
Cuts at: 242 1777 2025 2727 4223 242
Size: 1535 248 702 1496 365
Fragments arranged by size:
1535 1496 702 365 248
BsaWI w'CCGG_w
Cuts at: 471 1535 2056 2734 3008 3155 3566 471
Size: 1064 521 678 274 147 411 1251
Fragments arranged by size:
1251 1064 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 184 154
Size: 30 4316
BseYI C'CCAG_C
Cuts at: 1771 2529 3106 3602 1771
Size: 758 577 496 2515
Fragments arranged by size:
2515 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 1252
Size: 4346
BsiEI CG_ry'CG
Cuts at: 104 348 1888 1934 3142 104
Size: 244 1540 46 1208 1308
Fragments arranged by size:
1540 1308 1208 244 46
BsiHKAI G_wGCw'C
Cuts at: 830 1360 2138 2328 3120 830
Size: 530 778 190 792 2056
Fragments arranged by size:
2056 792 778 530 190
BsmI GAATG_Cn'
Cuts at: 910 1268 910
Size: 358 3988
Bsp1286I G_dGCh'C
Cuts at: 830 1360 1971 2064 2138 2328 2390 3120 830
Size: 530 611 93 74 190 62 730 2056
Fragments arranged by size:
2056 730 611 530 190 93 74 62
BspEI T'CCGG_A
Cuts at: 471 3566 471
Size: 3095 1251
BspHI T'CATG_A
Cuts at: 1098 3522 1098
Size: 2424 1922
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1396 1610 1912 2293 1396
Size: 214 302 381 3449
Fragments arranged by size:
3449 381 302 214
BsrBI CCG'CTC
Cuts at: 50 316 2638 2692 4176 4341 50
Size: 266 2322 54 1484 165 55
Fragments arranged by size:
2322 1484 266 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2258 2258
Size: 4346
BsrFI r'CCGG_y
Cuts at: 306 2344 2525 2734 306
Size: 2038 181 209 1918
Fragments arranged by size:
2038 1918 209 181
BssHII G'CGCG_C
Cuts at: 225 225
Size: 4346
BssSI C'ACGA_G
Cuts at: 2617 2975 2617
Size: 358 3988
BstAPI GCAn_nnn'nTGC
Cuts at: 1723 1723
Size: 4346
BstBI TT'CG_AA
Cuts at: 2707 2707
Size: 4346
BstXI CCAn_nnnn'nTGG
Cuts at: 375 790 375
Size: 415 3931
BstYI r'GATC_y
Cuts at: 1 372 456 1371 1838 1861 2196 2442 3454
Size: 371 84 915 467 23 335 246 1012
Cuts at: 3454 3540 3552 3898 1
Size: 86 12 346 449
Fragments arranged by size:
1012 915 467 449 371 346 335 246
86 84 23 12
BstZ17I GTA'TAC
Cuts at: 1187 1187
Size: 4346
BtgI C'CryG_G
Cuts at: 107 359 107
Size: 252 4094
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2296 2477 2640 2296
Size: 181 163 4002
Fragments arranged by size:
4002 181 163
BtsI GCAGTG_nn'
Cuts at: 183 1589 183
Size: 1406 2940
DraI TTT'AAA
Cuts at: 1345 1345
Size: 4346
DrdI GACnn_nn'nnGTC
Cuts at: 2052 2910 2052
Size: 858 3488
EaeI y'GGCC_r
Cuts at: 365 1931 2105 2496 2523 3868 365
Size: 1566 174 391 27 1345 843
Fragments arranged by size:
1566 1345 843 391 174 27
EagI C'GGCC_G
Cuts at: 1931 1931
Size: 4346
EarI CTCTTCn'nnn_
Cuts at: 2369 2579 2369
Size: 210 4136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2554 2864 3010 4156 2554
Size: 310 146 1146 2744
Fragments arranged by size:
2744 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1358 1358
Size: 4346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 409 766 2171 2603 3350 3618 134
Size: 275 357 1405 432 747 268 862
Fragments arranged by size:
1405 862 747 432 357 275 268
EcoO109I rG'GnC_Cy
Cuts at: 162 1230 1485 162
Size: 1068 255 3023
Fragments arranged by size:
3023 1068 255
EcoRI G'AATT_C
Cuts at: 796 1300 1350 796
Size: 504 50 3792
Fragments arranged by size:
3792 504 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 661 2365 661
Size: 1704 2642
FspI TGC'GCA
Cuts at: 2127 2127
Size: 4346
HaeII r_GCGC'y
Cuts at: 245 1780 2028 3050 4029 245
Size: 1535 248 1022 979 562
Fragments arranged by size:
1535 1022 979 562 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 571 603 1110 1142 2188 2218 2220
Size: 32 385 32 507 32 1046 30 2
Cuts at: 2220 2250 154
Size: 30 2250
Fragments arranged by size:
2250 1046 507 385 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1385 1385
Size: 4346
KasI G'GCGC_C
Cuts at: 241 1776 2024 241
Size: 1535 248 2563
Fragments arranged by size:
2563 1535 248
KpnI G_GTAC'C
Cuts at: 1366 1366
Size: 4346
MluI A'CGCG_T
Cuts at: 3779 3779
Size: 4346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 368 3017 3201 368
Size: 2649 184 1513
Fragments arranged by size:
2649 1513 184
MscI TGG'CCA
Cuts at: 367 2107 367
Size: 1740 2606
MslI CAynn'nnrTG
Cuts at: 1100 2335 2462 1100
Size: 1235 127 2984
Fragments arranged by size:
2984 1235 127
MspA1I CmG'CkG
Cuts at: 337 361 1457 1771 2131 3144 3389 337
Size: 24 1096 314 360 1013 245 1294
Fragments arranged by size:
1294 1096 1013 360 314 245 24
NaeI GCC'GGC
Cuts at: 308 2527 308
Size: 2219 2127
NarI GG'CG_CC
Cuts at: 242 1777 2025 242
Size: 1535 248 2563
Fragments arranged by size:
2563 1535 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4346
NgoMIV G'CCGG_C
Cuts at: 306 2525 306
Size: 2219 2127
NspI r_CATG'y
Cuts at: 595 1066 1399 1899 2806 595
Size: 471 333 500 907 2135
Fragments arranged by size:
2135 907 500 471 333
PciI A'CATG_T
Cuts at: 1062 2802 1062
Size: 1740 2606
PfoI T'CCnGG_A
Cuts at: 1326 1326
Size: 4346
PmeI GTTT'AAAC
Cuts at: 1345 1345
Size: 4346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 4314
PpuMI rG'GwC_Cy
Cuts at: 162 1230 162
Size: 1068 3278
PsiI TTA'TAA
Cuts at: 884 884
Size: 4346
PstI C_TGCA'G
Cuts at: 385 559 1393 2078 385
Size: 174 834 685 2653
Fragments arranged by size:
2653 834 685 174
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4346
PvuII CAG'CTG
Cuts at: 337 1771 2131 337
Size: 1434 360 2552
Fragments arranged by size:
2552 1434 360
RsrII CG'GwC_CG
Cuts at: 2541 2541
Size: 4346
SacI G_AGCT'C
Cuts at: 1360 1360
Size: 4346
SacII CC_GC'GG
Cuts at: 362 362
Size: 4346
SalI G'TCGA_C
Cuts at: 1383 1383
Size: 4346
SapI GCTCTTCn'nnn_
Cuts at: 2369 2579 2369
Size: 210 4136
SbfI CC_TGCA'GG
Cuts at: 1393 1393
Size: 4346
ScaI AGT'ACT
Cuts at: 3863 3863
Size: 4346
SexAI A'CCwGG_T
Cuts at: 485 485
Size: 4346
SfcI C'TryA_G
Cuts at: 33 381 555 1389 2074 3067 3258 33
Size: 348 174 834 685 993 191 1121
Fragments arranged by size:
1121 993 834 685 348 191 174
SfoI GGC'GCC
Cuts at: 243 1778 2026 243
Size: 1535 248 2563
Fragments arranged by size:
2563 1535 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
SmaI CCC'GGG
Cuts at: 1368 1368
Size: 4346
SmlI C'TyrA_G
Cuts at: 442 1501 2416 2908 3170 442
Size: 1059 915 492 262 1618
Fragments arranged by size:
1618 1059 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4346
SphI G_CATG'C
Cuts at: 1399 1899 1399
Size: 500 3846
StyI C'CwwG_G
Cuts at: 107 1480 1834 107
Size: 1373 354 2619
Fragments arranged by size:
2619 1373 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 139 1188 1929 2062 2521 2730 139
Size: 1049 741 133 459 209 1755
Fragments arranged by size:
1755 1049 741 459 209 133
TatI w'GTAC_w
Cuts at: 3861 3861
Size: 4346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3863 3895 3863
Size: 32 4314
Tth111I GACn'n_nGTC
Cuts at: 166 2143 166
Size: 1977 2369
XbaI T'CTAG_A
Cuts at: 1377 1377
Size: 4346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1841 1841
Size: 4346
XmaI C'CCGG_G
Cuts at: 1366 1366
Size: 4346
XmnI GAAnn'nnTTC
Cuts at: 467 467
Size: 4346
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI
BmtI BseRI BsiWI BsmBI BsrGI BstEII Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PspOMI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI