Restriction map for
pF1KB7971
(Circular) (Six-base) MAPSORT of: pF1KB7971.seq Check: 5605 from: 1 to: 3983
pF1KB7971 3983 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 252 1021 252
Size: 769 3214
Acc65I G'GTAC_C
Cuts at: 999 999
Size: 3983
AclI AA'CG_TT
Cuts at: 977 3823 977
Size: 2846 1137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1808 2240 2987 3255 1808
Size: 432 747 268 2536
Fragments arranged by size:
2536 747 432 268
AflIII A'CryG_T
Cuts at: 1335 2439 3416 1335
Size: 1104 977 1902
Fragments arranged by size:
1902 1104 977
AgeI A'CCGG_T
Cuts at: 2371 2371
Size: 3983
AleI CACnn'nnGTG
Cuts at: 760 760
Size: 3983
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 842 874 842
Size: 32 3951
AlwNI CAG_nnn'CTG
Cuts at: 507 556 1288 2855 507
Size: 49 732 1567 1635
Fragments arranged by size:
1635 1567 732 49
ApaI G_GGCC'C
Cuts at: 722 722
Size: 3983
ApaLI G'TGCA_C
Cuts at: 2753 2753
Size: 3983
ApoI r'AATT_y
Cuts at: 238 987 3336 3592 238
Size: 749 2349 256 629
Fragments arranged by size:
2349 749 629 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3983
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 199 1003 199
Size: 804 3179
BamHI G'GATC_C
Cuts at: 216 801 1008 216
Size: 585 207 3191
Fragments arranged by size:
3191 585 207
BanI G'GyrC_C
Cuts at: 999 1413 1661 1696 999
Size: 414 248 35 3286
Fragments arranged by size:
3286 414 248 35
BanII G_rGCy'C
Cuts at: 475 722 997 2027 475
Size: 247 275 1030 2431
Fragments arranged by size:
2431 1030 275 247
BbeI G_GCGC'C
Cuts at: 1417 1665 1417
Size: 248 3735
BbsI GAAGACnn'nnnn_
Cuts at: 341 341
Size: 3983
BciVI GTATCCnnnnn_n'
Cuts at: 309 1881 2642 3983 309
Size: 1572 761 1341 309
Fragments arranged by size:
1572 1341 761 309
BclI T'GATC_A
Cuts at: 270 1503 270
Size: 1233 2750
BglII A'GATC_T
Cuts at: 1498 3535 1498
Size: 2037 1946
BlpI GC'TnA_GC
Cuts at: 142 1095 142
Size: 953 3030
Bme1580I G_kGCm'C
Cuts at: 722 1608 1701 2757 722
Size: 886 93 1056 1948
Fragments arranged by size:
1948 1056 886 93
BmrI ACTGGGnnnn_n'
Cuts at: 527 1195 1276 1611 3755 527
Size: 668 81 335 2144 755
Fragments arranged by size:
2144 755 668 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 3983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 722 587
Size: 135 3848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 887 1159 2038 2530 2828 887
Size: 272 879 492 298 2042
Fragments arranged by size:
2042 879 492 298 272
BsaI GGTCTCn'nnnn_
Cuts at: 3687 3687
Size: 3983
BsaAI yAC'GTr
Cuts at: 1336 1336
Size: 3983
BsaBI GATnn'nnATC
Cuts at: 6 1497 3534 6
Size: 1491 2037 455
Fragments arranged by size:
2037 1491 455
BsaHI Gr'CG_yC
Cuts at: 1414 1662 2364 3860 1414
Size: 248 702 1496 1537
Fragments arranged by size:
1537 1496 702 248
BsaWI w'CCGG_w
Cuts at: 179 1172 1693 2371 2645 2792 3203 179
Size: 993 521 678 274 147 411 959
Fragments arranged by size:
993 959 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 161 191 481 511 161
Size: 30 290 30 3633
Fragments arranged by size:
3633 290 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 445 483 445
Size: 38 3945
BseYI C'CCAG_C
Cuts at: 832 1408 2166 2743 3239 832
Size: 576 758 577 496 1576
Fragments arranged by size:
1576 758 577 576 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 391
Size: 3983
BsiEI CG_ry'CG
Cuts at: 104 1525 1571 2779 104
Size: 1421 46 1208 1308
Fragments arranged by size:
1421 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 143 475 997 1775 1965 2757 143
Size: 332 522 778 190 792 1369
Fragments arranged by size:
1369 792 778 522 332 190
BsmI GAATG_Cn'
Cuts at: 401 401
Size: 3983
Bsp1286I G_dGCh'C
Cuts at: 143 475 722 997 1608 1701 1775 1965 2027
Size: 332 247 275 611 93 74 190 62
Cuts at: 2027 2757 143
Size: 730 1369
Fragments arranged by size:
1369 730 611 332 275 247 190 93
74 62
BspEI T'CCGG_A
Cuts at: 179 3203 179
Size: 3024 959
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1033 1247 1549 1930 1033
Size: 214 302 381 3086
Fragments arranged by size:
3086 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2275 2329 3813 3978 50
Size: 2225 54 1484 165 55
Fragments arranged by size:
2225 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 1895
Size: 3983
BsrFI r'CCGG_y
Cuts at: 1981 2162 2371 1981
Size: 181 209 3593
Fragments arranged by size:
3593 209 181
BssSI C'ACGA_G
Cuts at: 2254 2612 2254
Size: 358 3625
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 1360
Size: 3983
BstBI TT'CG_AA
Cuts at: 2344 2344
Size: 3983
BstYI r'GATC_y
Cuts at: 1 216 801 1008 1475 1498 1833 2079 3091
Size: 215 585 207 467 23 335 246 1012
Cuts at: 3091 3177 3189 3535 1
Size: 86 12 346 449
Fragments arranged by size:
1012 585 467 449 346 335 246 215
207 86 23 12
BtgI C'CryG_G
Cuts at: 107 441 107
Size: 334 3649
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1933 2114 2277 1933
Size: 181 163 3639
Fragments arranged by size:
3639 181 163
BtsI GCAGTG_nn'
Cuts at: 385 767 1226 385
Size: 382 459 3142
Fragments arranged by size:
3142 459 382
DraI TTT'AAA
Cuts at: 243 982 243
Size: 739 3244
DrdI GACnn_nn'nnGTC
Cuts at: 1689 2547 1689
Size: 858 3125
EaeI y'GGCC_r
Cuts at: 1568 1742 2133 2160 3505 1568
Size: 174 391 27 1345 2046
Fragments arranged by size:
2046 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1568 1568
Size: 3983
EarI CTCTTCn'nnn_
Cuts at: 887 2006 2216 887
Size: 1119 210 2654
Fragments arranged by size:
2654 1119 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2191 2501 2647 3793 2191
Size: 310 146 1146 2381
Fragments arranged by size:
2381 1146 310 146
EcoICRI GAG'CTC
Cuts at: 473 995 473
Size: 522 3461
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 722 1808 2240 2987 3255 587
Size: 135 1086 432 747 268 1315
Fragments arranged by size:
1315 1086 747 432 268 135
EcoO109I rG'GnC_Cy
Cuts at: 113 1122 113
Size: 1009 2974
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
EcoRV GAT'ATC
Cuts at: 698 698
Size: 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 928 2002 928
Size: 1074 2909
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 1417 1665 2687 3666 1417
Size: 248 1022 979 1734
Fragments arranged by size:
1734 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 161 193 1825 1855 1857 1887 161
Size: 32 1632 30 2 30 2257
Fragments arranged by size:
2257 1632 32 30 30 2
HincII GTy'rAC
Cuts at: 1022 1022
Size: 3983
HindIII A'AGCT_T
Cuts at: 681 681
Size: 3983
KasI G'GCGC_C
Cuts at: 1413 1661 1413
Size: 248 3735
KpnI G_GTAC'C
Cuts at: 1003 1003
Size: 3983
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 137 147 885 2654 2838 137
Size: 10 738 1769 184 1282
Fragments arranged by size:
1769 1282 738 184 10
MscI TGG'CCA
Cuts at: 1744 1744
Size: 3983
MslI CAynn'nnrTG
Cuts at: 760 1972 2099 760
Size: 1212 127 2644
Fragments arranged by size:
2644 1212 127
MspA1I CmG'CkG
Cuts at: 1094 1408 1768 2781 3026 1094
Size: 314 360 1013 245 2051
Fragments arranged by size:
2051 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2164 2164
Size: 3983
NarI GG'CG_CC
Cuts at: 1414 1662 1414
Size: 248 3735
NcoI C'CATG_G
Cuts at: 107 441 107
Size: 334 3649
NgoMIV G'CCGG_C
Cuts at: 2162 2162
Size: 3983
NspI r_CATG'y
Cuts at: 1036 1536 2443 1036
Size: 500 907 2576
Fragments arranged by size:
2576 907 500
PasI CC'CwG_GG
Cuts at: 576 576
Size: 3983
PciI A'CATG_T
Cuts at: 2439 2439
Size: 3983
PflMI CCAn_nnn'nTGG
Cuts at: 442 956 442
Size: 514 3469
PfoI T'CCnGG_A
Cuts at: 308 308
Size: 3983
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 3951
PpuMI rG'GwC_Cy
Cuts at: 113 113
Size: 3983
PspOMI G'GGCC_C
Cuts at: 718 718
Size: 3983
PstI C_TGCA'G
Cuts at: 1030 1715 1030
Size: 685 3298
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3983
PvuII CAG'CTG
Cuts at: 1408 1768 1408
Size: 360 3623
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 475 997 475
Size: 522 3461
SalI G'TCGA_C
Cuts at: 1020 1020
Size: 3983
SapI GCTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
SbfI CC_TGCA'GG
Cuts at: 1030 1030
Size: 3983
ScaI AGT'ACT
Cuts at: 3500 3500
Size: 3983
SfcI C'TryA_G
Cuts at: 33 1026 1711 2704 2895 33
Size: 993 685 993 191 1121
Fragments arranged by size:
1121 993 993 685 191
SfoI GGC'GCC
Cuts at: 1415 1663 1415
Size: 248 3735
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SmaI CCC'GGG
Cuts at: 1005 1005
Size: 3983
SmlI C'TyrA_G
Cuts at: 902 1138 2053 2545 2807 902
Size: 236 915 492 262 2078
Fragments arranged by size:
2078 915 492 262 236
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 1036 1536 1036
Size: 500 3483
StyI C'CwwG_G
Cuts at: 107 441 590 1117 1471 107
Size: 334 149 527 354 2619
Fragments arranged by size:
2619 527 354 334 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1566 1699 2158 2367 1566
Size: 133 459 209 3182
Fragments arranged by size:
3182 459 209 133
TatI w'GTAC_w
Cuts at: 3498 3498
Size: 3983
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 3951
Tth111I GACn'n_nGTC
Cuts at: 781 1780 781
Size: 999 2984
XbaI T'CTAG_A
Cuts at: 1014 1014
Size: 3983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1478 1478
Size: 3983
XmaI C'CCGG_G
Cuts at: 1003 1003
Size: 3983
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI