Restriction map for
pF1KB7967
(Circular) (Six-base) MAPSORT of: pF1KB7967.seq Check: 4219 from: 1 to: 3962
pF1KB7967 3962 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1000 1000
Size: 3962
Acc65I G'GTAC_C
Cuts at: 978 978
Size: 3962
AclI AA'CG_TT
Cuts at: 228 3802 228
Size: 3574 388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1787 2219 2966 3234 364
Size: 1423 432 747 268 1092
Fragments arranged by size:
1423 1092 747 432 268
AflIII A'CryG_T
Cuts at: 1314 2418 3395 1314
Size: 1104 977 1881
Fragments arranged by size:
1881 1104 977
AgeI A'CCGG_T
Cuts at: 707 2350 707
Size: 1643 2319
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 218 250 218
Size: 32 3930
AlwNI CAG_nnn'CTG
Cuts at: 1267 2834 1267
Size: 1567 2395
ApaLI G'TGCA_C
Cuts at: 154 703 2732 154
Size: 549 2029 1384
Fragments arranged by size:
2029 1384 549
ApoI r'AATT_y
Cuts at: 274 966 3315 3571 274
Size: 692 2349 256 665
Fragments arranged by size:
2349 692 665 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3962
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
AvaI C'yCGr_G
Cuts at: 317 384 516 604 982 317
Size: 67 132 88 378 3297
Fragments arranged by size:
3297 378 132 88 67
BamHI G'GATC_C
Cuts at: 987 987
Size: 3962
BanI G'GyrC_C
Cuts at: 710 950 978 1392 1640 1675 710
Size: 240 28 414 248 35 2997
Fragments arranged by size:
2997 414 248 240 35 28
BanII G_rGCy'C
Cuts at: 146 248 532 976 2006 146
Size: 102 284 444 1030 2102
Fragments arranged by size:
2102 1030 444 284 102
BbeI G_GCGC'C
Cuts at: 1396 1644 1396
Size: 248 3714
BciVI GTATCCnnnnn_n'
Cuts at: 1860 2621 3962 1860
Size: 761 1341 1860
Fragments arranged by size:
1860 1341 761
BclI T'GATC_A
Cuts at: 1482 1482
Size: 3962
BglII A'GATC_T
Cuts at: 291 1477 3514 291
Size: 1186 2037 739
Fragments arranged by size:
2037 1186 739
BlpI GC'TnA_GC
Cuts at: 1074 1074
Size: 3962
Bme1580I G_kGCm'C
Cuts at: 158 605 707 1587 1680 2736 158
Size: 447 102 880 93 1056 1384
Fragments arranged by size:
1384 1056 880 447 102 93
BmgBI CAC'GTC
Cuts at: 114 114
Size: 3962
BmrI ACTGGGnnnn_n'
Cuts at: 1174 1255 1590 3734 1174
Size: 81 335 2144 1402
Fragments arranged by size:
2144 1402 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 633 2329 633
Size: 1696 2266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 211 220 347 130
Size: 81 9 127 3745
Fragments arranged by size:
3745 127 81 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 2017 2509 2807 1138
Size: 879 492 298 2293
Fragments arranged by size:
2293 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 154 400 3666 154
Size: 246 3266 450
Fragments arranged by size:
3266 450 246
BsaAI yAC'GTr
Cuts at: 1315 1315
Size: 3962
BsaBI GATnn'nnATC
Cuts at: 6 1476 3513 6
Size: 1470 2037 455
Fragments arranged by size:
2037 1470 455
BsaHI Gr'CG_yC
Cuts at: 1393 1641 2343 3839 1393
Size: 248 702 1496 1516
Fragments arranged by size:
1516 1496 702 248
BsaWI w'CCGG_w
Cuts at: 561 707 1151 1672 2350 2624 2771 3182 561
Size: 146 444 521 678 274 147 411 1341
Fragments arranged by size:
1341 678 521 444 411 274 147 146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 218 248 218
Size: 30 3932
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 522 711 208
Size: 314 189 3459
Fragments arranged by size:
3459 314 189
BseYI C'CCAG_C
Cuts at: 1387 2145 2722 3218 1387
Size: 758 577 496 2131
Fragments arranged by size:
2131 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 922 147
Size: 775 3187
BsiEI CG_ry'CG
Cuts at: 104 1504 1550 2758 104
Size: 1400 46 1208 1308
Fragments arranged by size:
1400 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 119 146 158 532 707 976 1754 1944 2736
Size: 27 12 374 175 269 778 190 792
Cuts at: 2736 119
Size: 1345
Fragments arranged by size:
1345 792 778 374 269 190 175 27
12
BsmBI CGTCTCn'nnnn_
Cuts at: 191 191
Size: 3962
Bsp1286I G_dGCh'C
Cuts at: 119 146 158 248 532 605 707 976 1587
Size: 27 12 90 284 73 102 269 611
Cuts at: 1587 1680 1754 1944 2006 2736 119
Size: 93 74 190 62 730 1345
Fragments arranged by size:
1345 730 611 284 269 190 102 93
90 74 73 62 27 12
BspEI T'CCGG_A
Cuts at: 561 3182 561
Size: 2621 1341
BspHI T'CATG_A
Cuts at: 3138 3138
Size: 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 220 1012 1226 1528 1909 220
Size: 792 214 302 381 2273
Fragments arranged by size:
2273 792 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2254 2308 3792 3957 50
Size: 2204 54 1484 165 55
Fragments arranged by size:
2204 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1874 1874
Size: 3962
BsrFI r'CCGG_y
Cuts at: 163 707 1960 2141 2350 163
Size: 544 1253 181 209 1775
Fragments arranged by size:
1775 1253 544 209 181
BssSI C'ACGA_G
Cuts at: 157 2233 2591 157
Size: 2076 358 1528
Fragments arranged by size:
2076 1528 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1339 1339
Size: 3962
BstBI TT'CG_AA
Cuts at: 2323 2323
Size: 3962
BstYI r'GATC_y
Cuts at: 1 291 987 1454 1477 1812 2058 3070 3156
Size: 290 696 467 23 335 246 1012 86
Cuts at: 3156 3168 3514 1
Size: 12 346 449
Fragments arranged by size:
1012 696 467 449 346 335 290 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 470 107
Size: 363 3599
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 689 1912 2093 2256 689
Size: 1223 181 163 2395
Fragments arranged by size:
2395 1223 181 163
BtsI GCAGTG_nn'
Cuts at: 1205 1205
Size: 3962
DraI TTT'AAA
Cuts at: 888 961 888
Size: 73 3889
DrdI GACnn_nn'nnGTC
Cuts at: 1668 2526 1668
Size: 858 3104
EaeI y'GGCC_r
Cuts at: 473 1547 1721 2112 2139 3484 473
Size: 1074 174 391 27 1345 951
Fragments arranged by size:
1345 1074 951 391 174 27
EagI C'GGCC_G
Cuts at: 1547 1547
Size: 3962
EarI CTCTTCn'nnn_
Cuts at: 940 1985 2195 940
Size: 1045 210 2707
Fragments arranged by size:
2707 1045 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2170 2480 2626 3772 2170
Size: 310 146 1146 2360
Fragments arranged by size:
2360 1146 310 146
EcoICRI GAG'CTC
Cuts at: 144 530 974 144
Size: 386 444 3132
Fragments arranged by size:
3132 444 386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 211 220 347 364 1787 2219 2966 3234
Size: 81 9 127 17 1423 432 747 268
Cuts at: 3234 130
Size: 858
Fragments arranged by size:
1423 858 747 432 268 127 81 17
9
EcoO109I rG'GnC_Cy
Cuts at: 1101 1101
Size: 3962
EcoRI G'AATT_C
Cuts at: 966 966
Size: 3962
EcoRV GAT'ATC
Cuts at: 350 350
Size: 3962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1981 1981
Size: 3962
FspI TGC'GCA
Cuts at: 634 1743 634
Size: 1109 2853
HaeII r_GCGC'y
Cuts at: 181 1396 1644 2666 3645 181
Size: 1215 248 1022 979 498
Fragments arranged by size:
1215 1022 979 498 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1804 1834 1836 1866 1804
Size: 30 2 30 3900
Fragments arranged by size:
3900 30 30 2
HincII GTy'rAC
Cuts at: 848 1001 848
Size: 153 3809
HpaI GTT'AAC
Cuts at: 848 848
Size: 3962
KasI G'GCGC_C
Cuts at: 1392 1640 1392
Size: 248 3714
KpnI G_GTAC'C
Cuts at: 982 982
Size: 3962
MluI A'CGCG_T
Cuts at: 3395 3395
Size: 3962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 512 2633 2817 512
Size: 2121 184 1657
Fragments arranged by size:
2121 1657 184
MscI TGG'CCA
Cuts at: 1723 1723
Size: 3962
MslI CAynn'nnrTG
Cuts at: 112 1951 2078 112
Size: 1839 127 1996
Fragments arranged by size:
1996 1839 127
MspA1I CmG'CkG
Cuts at: 638 771 1073 1387 1747 2760 3005 638
Size: 133 302 314 360 1013 245 1595
Fragments arranged by size:
1595 1013 360 314 302 245 133
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 3962
NarI GG'CG_CC
Cuts at: 1393 1641 1393
Size: 248 3714
NcoI C'CATG_G
Cuts at: 107 107
Size: 3962
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 3962
NspI r_CATG'y
Cuts at: 1015 1515 2422 1015
Size: 500 907 2555
Fragments arranged by size:
2555 907 500
PciI A'CATG_T
Cuts at: 2418 2418
Size: 3962
PfoI T'CCnGG_A
Cuts at: 137 137
Size: 3962
PmeI GTTT'AAAC
Cuts at: 961 961
Size: 3962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 218 250 3101 3133 218
Size: 32 2851 32 1047
Fragments arranged by size:
2851 1047 32 32
PstI C_TGCA'G
Cuts at: 730 1009 1694 730
Size: 279 685 2998
Fragments arranged by size:
2998 685 279
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3962
PvuII CAG'CTG
Cuts at: 1387 1747 1387
Size: 360 3602
RsrII CG'GwC_CG
Cuts at: 2157 2157
Size: 3962
SacI G_AGCT'C
Cuts at: 146 532 976 146
Size: 386 444 3132
Fragments arranged by size:
3132 444 386
SalI G'TCGA_C
Cuts at: 999 999
Size: 3962
SapI GCTCTTCn'nnn_
Cuts at: 1985 2195 1985
Size: 210 3752
SbfI CC_TGCA'GG
Cuts at: 1009 1009
Size: 3962
ScaI AGT'ACT
Cuts at: 199 3479 199
Size: 3280 682
SfcI C'TryA_G
Cuts at: 33 726 1005 1690 2683 2874 33
Size: 693 279 685 993 191 1121
Fragments arranged by size:
1121 993 693 685 279 191
SfoI GGC'GCC
Cuts at: 1394 1642 1394
Size: 248 3714
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
SgrAI Cr'CCGG_yG
Cuts at: 707 707
Size: 3962
SmaI CCC'GGG
Cuts at: 606 984 606
Size: 378 3584
SmlI C'TyrA_G
Cuts at: 1117 2032 2524 2786 1117
Size: 915 492 262 2293
Fragments arranged by size:
2293 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3962
SphI G_CATG'C
Cuts at: 1015 1515 1015
Size: 500 3462
StyI C'CwwG_G
Cuts at: 107 1096 1450 107
Size: 989 354 2619
Fragments arranged by size:
2619 989 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1545 1678 2137 2346 1545
Size: 133 459 209 3161
Fragments arranged by size:
3161 459 209 133
TatI w'GTAC_w
Cuts at: 197 3477 197
Size: 3280 682
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 280 312 642 674 3479 3511 280
Size: 32 330 32 2805 32 731
Fragments arranged by size:
2805 731 330 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1759 1759
Size: 3962
XbaI T'CTAG_A
Cuts at: 993 993
Size: 3962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 309 1457 309
Size: 1148 2814
XmaI C'CCGG_G
Cuts at: 604 982 604
Size: 378 3584
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI